The Experts below are selected from a list of 78 Experts worldwide ranked by ideXlab platform
Ken Ichiro Hata - One of the best experts on this subject based on the ideXlab platform.
-
Establishment of a new in vitro test method for evaluation of Eye irritancy using a reconstructed human corneal epithelial model, LabCyte CORNEA-MODEL.
Toxicology in Vitro, 2013Co-Authors: Masakazu Katoh, Fumiyasu Hamajima, Takahiro Ogasawara, Ken Ichiro HataAbstract:Abstract Finding in vitro Eye irritation testing alternatives to animal testing such as the Draize Eye test, which uses rabbits, is essential from the standpoint of animal welfare. It has been developed a reconstructed human corneal epithelial model, the LabCyte CORNEA-MODEL, which has a representative corneal epithelium-like structure. Protocol optimization (pre-validation study) was examined in order to establish a new alternative method for Eye irritancy evaluation with this model. From the results of the optimization experiments, the application periods for chemicals were set at 1 min for liquid chemicals or 24 h for solid chemicals, and the post-exposure incubation periods were set at 24 h for liquids or zero for solids. If the viability was less than 50%, the chemical was judged to be an Eye Irritant. Sixty-one chemicals were applied in the optimized protocol using the LabCyte CORNEA-MODEL and these results were evaluated in correlation with in vivo results. The predictions of the optimized LabCyte CORNEA-MODEL Eye irritation test methods were highly correlated with in vivo Eye irritation (sensitivity 100%, specificity 80.0%, and accuracy 91.8%). These results suggest that the LabCyte CORNEA-MODEL Eye irritation test could be useful as an alternative method to the Draize Eye test.
-
Establishment of a new in vitro test method for evaluation of Eye irritancy using a reconstructed human corneal epithelial model, LabCyte CORNEA-MODEL
Toxicology in Vitro, 2013Co-Authors: Masakazu Katoh, Fumiyasu Hamajima, Takahiro Ogasawara, Ken Ichiro HataAbstract:Finding in vitro Eye irritation testing alternatives to animal testing such as the Draize Eye test, which uses rabbits, is essential from the standpoint of animal welfare. It has been developed a reconstructed human corneal epithelial model, the LabCyte CORNEA-MODEL, which has a representative corneal epithelium-like structure. Protocol optimization (pre-validation study) was examined in order to establish a new alternative method for Eye irritancy evaluation with this model. From the results of the optimization experiments, the application periods for chemicals were set at 1. min for liquid chemicals or 24. h for solid chemicals, and the post-exposure incubation periods were set at 24. h for liquids or zero for solids. If the viability was less than 50%, the chemical was judged to be an Eye Irritant. Sixty-one chemicals were applied in the optimized protocol using the LabCyte CORNEA-MODEL and these results were evaluated in correlation with in vivo results. The predictions of the optimized LabCyte CORNEA-MODEL Eye irritation test methods were highly correlated with in vivo Eye irritation (sensitivity 100%, specificity 80.0%, and accuracy 91.8%). These results suggest that the LabCyte CORNEA-MODEL Eye irritation test could be useful as an alternative method to the Draize Eye test. © 2013 Elsevier Ltd.
Masakazu Katoh - One of the best experts on this subject based on the ideXlab platform.
-
Establishment of a new in vitro test method for evaluation of Eye irritancy using a reconstructed human corneal epithelial model, LabCyte CORNEA-MODEL.
Toxicology in Vitro, 2013Co-Authors: Masakazu Katoh, Fumiyasu Hamajima, Takahiro Ogasawara, Ken Ichiro HataAbstract:Abstract Finding in vitro Eye irritation testing alternatives to animal testing such as the Draize Eye test, which uses rabbits, is essential from the standpoint of animal welfare. It has been developed a reconstructed human corneal epithelial model, the LabCyte CORNEA-MODEL, which has a representative corneal epithelium-like structure. Protocol optimization (pre-validation study) was examined in order to establish a new alternative method for Eye irritancy evaluation with this model. From the results of the optimization experiments, the application periods for chemicals were set at 1 min for liquid chemicals or 24 h for solid chemicals, and the post-exposure incubation periods were set at 24 h for liquids or zero for solids. If the viability was less than 50%, the chemical was judged to be an Eye Irritant. Sixty-one chemicals were applied in the optimized protocol using the LabCyte CORNEA-MODEL and these results were evaluated in correlation with in vivo results. The predictions of the optimized LabCyte CORNEA-MODEL Eye irritation test methods were highly correlated with in vivo Eye irritation (sensitivity 100%, specificity 80.0%, and accuracy 91.8%). These results suggest that the LabCyte CORNEA-MODEL Eye irritation test could be useful as an alternative method to the Draize Eye test.
-
Establishment of a new in vitro test method for evaluation of Eye irritancy using a reconstructed human corneal epithelial model, LabCyte CORNEA-MODEL
Toxicology in Vitro, 2013Co-Authors: Masakazu Katoh, Fumiyasu Hamajima, Takahiro Ogasawara, Ken Ichiro HataAbstract:Finding in vitro Eye irritation testing alternatives to animal testing such as the Draize Eye test, which uses rabbits, is essential from the standpoint of animal welfare. It has been developed a reconstructed human corneal epithelial model, the LabCyte CORNEA-MODEL, which has a representative corneal epithelium-like structure. Protocol optimization (pre-validation study) was examined in order to establish a new alternative method for Eye irritancy evaluation with this model. From the results of the optimization experiments, the application periods for chemicals were set at 1. min for liquid chemicals or 24. h for solid chemicals, and the post-exposure incubation periods were set at 24. h for liquids or zero for solids. If the viability was less than 50%, the chemical was judged to be an Eye Irritant. Sixty-one chemicals were applied in the optimized protocol using the LabCyte CORNEA-MODEL and these results were evaluated in correlation with in vivo results. The predictions of the optimized LabCyte CORNEA-MODEL Eye irritation test methods were highly correlated with in vivo Eye irritation (sensitivity 100%, specificity 80.0%, and accuracy 91.8%). These results suggest that the LabCyte CORNEA-MODEL Eye irritation test could be useful as an alternative method to the Draize Eye test. © 2013 Elsevier Ltd.
Pichao Wang - One of the best experts on this subject based on the ideXlab platform.
-
development of a human corneal epithelium model utilizing a collagen vitrigel membrane and the changes of its barrier function induced by exposing Eye Irritant chemicals
Toxicology in Vitro, 2011Co-Authors: Toshiaki Takezawa, Kazunori Nishikawa, Pichao WangAbstract:Abstract The brief TEER (trans-epithelial electrical resistance) assay after exposing chemicals to corneal epithelium in vivo is known as a suitable method for evaluating corneal irritancy and permeability quantitatively and continuously. A collagen vitrigel membrane we previously developed is a thin (about 20 μm thick) and transparent membrane composed of high density collagen fibrils equivalent to connective tissues in vivo, e.g. corneal Bowman’s membrane. To develop such a TEER assay system in vitro utilizing a human corneal epithelial model, HCE-T cells (a human corneal epithelial cell line) were cultured on the collagen vitrigel membrane substratum prepared in a Millicell chamber suitable for TEER measurement. Human corneal epithelium model possessing 5–6 cell layers sufficient for TEER assay was successfully reconstructed on the substratum in the Millicell chamber by culturing the cells in monolayer for 2 days and subsequently in air–liquid interface for 7 days. The exposure of chemicals to the model induced the time-dependent relative changes of TEER in response to the characteristic of each chemical within a few minutes. These results suggest that the TEER assay using the human corneal epithelial model is very useful for an ocular irritancy evaluation as an alternative to the Draize Eye irritation test.
Fumiyasu Hamajima - One of the best experts on this subject based on the ideXlab platform.
-
Establishment of a new in vitro test method for evaluation of Eye irritancy using a reconstructed human corneal epithelial model, LabCyte CORNEA-MODEL.
Toxicology in Vitro, 2013Co-Authors: Masakazu Katoh, Fumiyasu Hamajima, Takahiro Ogasawara, Ken Ichiro HataAbstract:Abstract Finding in vitro Eye irritation testing alternatives to animal testing such as the Draize Eye test, which uses rabbits, is essential from the standpoint of animal welfare. It has been developed a reconstructed human corneal epithelial model, the LabCyte CORNEA-MODEL, which has a representative corneal epithelium-like structure. Protocol optimization (pre-validation study) was examined in order to establish a new alternative method for Eye irritancy evaluation with this model. From the results of the optimization experiments, the application periods for chemicals were set at 1 min for liquid chemicals or 24 h for solid chemicals, and the post-exposure incubation periods were set at 24 h for liquids or zero for solids. If the viability was less than 50%, the chemical was judged to be an Eye Irritant. Sixty-one chemicals were applied in the optimized protocol using the LabCyte CORNEA-MODEL and these results were evaluated in correlation with in vivo results. The predictions of the optimized LabCyte CORNEA-MODEL Eye irritation test methods were highly correlated with in vivo Eye irritation (sensitivity 100%, specificity 80.0%, and accuracy 91.8%). These results suggest that the LabCyte CORNEA-MODEL Eye irritation test could be useful as an alternative method to the Draize Eye test.
-
Establishment of a new in vitro test method for evaluation of Eye irritancy using a reconstructed human corneal epithelial model, LabCyte CORNEA-MODEL
Toxicology in Vitro, 2013Co-Authors: Masakazu Katoh, Fumiyasu Hamajima, Takahiro Ogasawara, Ken Ichiro HataAbstract:Finding in vitro Eye irritation testing alternatives to animal testing such as the Draize Eye test, which uses rabbits, is essential from the standpoint of animal welfare. It has been developed a reconstructed human corneal epithelial model, the LabCyte CORNEA-MODEL, which has a representative corneal epithelium-like structure. Protocol optimization (pre-validation study) was examined in order to establish a new alternative method for Eye irritancy evaluation with this model. From the results of the optimization experiments, the application periods for chemicals were set at 1. min for liquid chemicals or 24. h for solid chemicals, and the post-exposure incubation periods were set at 24. h for liquids or zero for solids. If the viability was less than 50%, the chemical was judged to be an Eye Irritant. Sixty-one chemicals were applied in the optimized protocol using the LabCyte CORNEA-MODEL and these results were evaluated in correlation with in vivo results. The predictions of the optimized LabCyte CORNEA-MODEL Eye irritation test methods were highly correlated with in vivo Eye irritation (sensitivity 100%, specificity 80.0%, and accuracy 91.8%). These results suggest that the LabCyte CORNEA-MODEL Eye irritation test could be useful as an alternative method to the Draize Eye test. © 2013 Elsevier Ltd.
Takahiro Ogasawara - One of the best experts on this subject based on the ideXlab platform.
-
Establishment of a new in vitro test method for evaluation of Eye irritancy using a reconstructed human corneal epithelial model, LabCyte CORNEA-MODEL.
Toxicology in Vitro, 2013Co-Authors: Masakazu Katoh, Fumiyasu Hamajima, Takahiro Ogasawara, Ken Ichiro HataAbstract:Abstract Finding in vitro Eye irritation testing alternatives to animal testing such as the Draize Eye test, which uses rabbits, is essential from the standpoint of animal welfare. It has been developed a reconstructed human corneal epithelial model, the LabCyte CORNEA-MODEL, which has a representative corneal epithelium-like structure. Protocol optimization (pre-validation study) was examined in order to establish a new alternative method for Eye irritancy evaluation with this model. From the results of the optimization experiments, the application periods for chemicals were set at 1 min for liquid chemicals or 24 h for solid chemicals, and the post-exposure incubation periods were set at 24 h for liquids or zero for solids. If the viability was less than 50%, the chemical was judged to be an Eye Irritant. Sixty-one chemicals were applied in the optimized protocol using the LabCyte CORNEA-MODEL and these results were evaluated in correlation with in vivo results. The predictions of the optimized LabCyte CORNEA-MODEL Eye irritation test methods were highly correlated with in vivo Eye irritation (sensitivity 100%, specificity 80.0%, and accuracy 91.8%). These results suggest that the LabCyte CORNEA-MODEL Eye irritation test could be useful as an alternative method to the Draize Eye test.
-
Establishment of a new in vitro test method for evaluation of Eye irritancy using a reconstructed human corneal epithelial model, LabCyte CORNEA-MODEL
Toxicology in Vitro, 2013Co-Authors: Masakazu Katoh, Fumiyasu Hamajima, Takahiro Ogasawara, Ken Ichiro HataAbstract:Finding in vitro Eye irritation testing alternatives to animal testing such as the Draize Eye test, which uses rabbits, is essential from the standpoint of animal welfare. It has been developed a reconstructed human corneal epithelial model, the LabCyte CORNEA-MODEL, which has a representative corneal epithelium-like structure. Protocol optimization (pre-validation study) was examined in order to establish a new alternative method for Eye irritancy evaluation with this model. From the results of the optimization experiments, the application periods for chemicals were set at 1. min for liquid chemicals or 24. h for solid chemicals, and the post-exposure incubation periods were set at 24. h for liquids or zero for solids. If the viability was less than 50%, the chemical was judged to be an Eye Irritant. Sixty-one chemicals were applied in the optimized protocol using the LabCyte CORNEA-MODEL and these results were evaluated in correlation with in vivo results. The predictions of the optimized LabCyte CORNEA-MODEL Eye irritation test methods were highly correlated with in vivo Eye irritation (sensitivity 100%, specificity 80.0%, and accuracy 91.8%). These results suggest that the LabCyte CORNEA-MODEL Eye irritation test could be useful as an alternative method to the Draize Eye test. © 2013 Elsevier Ltd.
