The Experts below are selected from a list of 396 Experts worldwide ranked by ideXlab platform
Antonia Mallardi - One of the best experts on this subject based on the ideXlab platform.
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three immobilized enzymes acting in series in layer by layer assemblies exploiting the trehalase glucose oxidase horseradish peroxidase cascade reactions for the optical determination of trehalose
Sensors and Actuators B-chemical, 2014Co-Authors: Gerardo Palazzo, Giuseppe Colafemmina, Claudia Guzzoni Iudice, Antonia MallardiAbstract:Abstract A new optical biosensor for trehalose determination has been realized immobilizing three glycoenzymes on a transparent support. Trehalase, glucose oxidase and horseradish peroxidase have been alternated with layers of Concanavalin A by a “layer-by-layer” (LbL) deposition. The driving force of this assembly is the biospecific complexation between Concanavalin A and sugar residues in the glycoenzymes. As confirmed by UV–vis spectroscopy, the LbL deposition allowed a high ordinate architecture with high loading of enzymes. After the assembly, the functionality of immobilized enzymes was spectrophotometrically proven, demonstrating also that they can act in series catalyzing cascade reactions. The prepared biosensor was used to optically detect trehalose, giving a LOD of 10 μM and a linear response up to 4 mM, and it showed also good time stability. The trehalose content in a real sample (Eyewash) was successfully determined by the biosensor.
Nan Liang - One of the best experts on this subject based on the ideXlab platform.
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Application of disposable blood transfusion apparatus instead of traditional Eyewash equipment
Chinese Journal of Modern Nursing, 2017Co-Authors: Xiukun Wang, Bing Wang, Tong Gao, Junxia Bai, Shuo Liu, Nan LiangAbstract:Objective To explore the clinical effect of using disposable blood transfusion apparatus as Eyewash equipment. Methods The clinical effect of Eyewash equipment that changed from disposable blood transfusion apparatus was compared with that of traditional Eyewash equipment. Results Compared with the traditional Eyewash equipment, the time of eye wash was decreased from five minutes to two minutes; the number of nurses responsible for washing every day was reduced from four to two; incidence rate of complications related to bacterial endophthalmitis was reduced from 0.10‰ to 0.05 ‰; error rate was dropped from 0.130‰ to 0.024‰; complaint rate was reduced from 0.087‰ to 0.012‰ (P
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application of disposable blood transfusion apparatus instead of traditional Eyewash equipment
Chinese Journal of Modern Nursing, 2017Co-Authors: Xiukun Wang, Bing Wang, Tong Gao, Junxia Bai, Shuo Liu, Nan LiangAbstract:Objective To explore the clinical effect of using disposable blood transfusion apparatus as Eyewash equipment. Methods The clinical effect of Eyewash equipment that changed from disposable blood transfusion apparatus was compared with that of traditional Eyewash equipment. Results Compared with the traditional Eyewash equipment, the time of eye wash was decreased from five minutes to two minutes; the number of nurses responsible for washing every day was reduced from four to two; incidence rate of complications related to bacterial endophthalmitis was reduced from 0.10‰ to 0.05 ‰; error rate was dropped from 0.130‰ to 0.024‰; complaint rate was reduced from 0.087‰ to 0.012‰ (P<0.05) . Conclusions Application of disposable blood transfusion apparatus in eye washing is effect and safe, it can reduce the incidence of complications and improve the satisfaction of patients and medical staff. Key words: Eyewash equipment; Cataract surgery; Complications
Gerardo Palazzo - One of the best experts on this subject based on the ideXlab platform.
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three immobilized enzymes acting in series in layer by layer assemblies exploiting the trehalase glucose oxidase horseradish peroxidase cascade reactions for the optical determination of trehalose
Sensors and Actuators B-chemical, 2014Co-Authors: Gerardo Palazzo, Giuseppe Colafemmina, Claudia Guzzoni Iudice, Antonia MallardiAbstract:Abstract A new optical biosensor for trehalose determination has been realized immobilizing three glycoenzymes on a transparent support. Trehalase, glucose oxidase and horseradish peroxidase have been alternated with layers of Concanavalin A by a “layer-by-layer” (LbL) deposition. The driving force of this assembly is the biospecific complexation between Concanavalin A and sugar residues in the glycoenzymes. As confirmed by UV–vis spectroscopy, the LbL deposition allowed a high ordinate architecture with high loading of enzymes. After the assembly, the functionality of immobilized enzymes was spectrophotometrically proven, demonstrating also that they can act in series catalyzing cascade reactions. The prepared biosensor was used to optically detect trehalose, giving a LOD of 10 μM and a linear response up to 4 mM, and it showed also good time stability. The trehalose content in a real sample (Eyewash) was successfully determined by the biosensor.
Xiukun Wang - One of the best experts on this subject based on the ideXlab platform.
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Application of disposable blood transfusion apparatus instead of traditional Eyewash equipment
Chinese Journal of Modern Nursing, 2017Co-Authors: Xiukun Wang, Bing Wang, Tong Gao, Junxia Bai, Shuo Liu, Nan LiangAbstract:Objective To explore the clinical effect of using disposable blood transfusion apparatus as Eyewash equipment. Methods The clinical effect of Eyewash equipment that changed from disposable blood transfusion apparatus was compared with that of traditional Eyewash equipment. Results Compared with the traditional Eyewash equipment, the time of eye wash was decreased from five minutes to two minutes; the number of nurses responsible for washing every day was reduced from four to two; incidence rate of complications related to bacterial endophthalmitis was reduced from 0.10‰ to 0.05 ‰; error rate was dropped from 0.130‰ to 0.024‰; complaint rate was reduced from 0.087‰ to 0.012‰ (P
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application of disposable blood transfusion apparatus instead of traditional Eyewash equipment
Chinese Journal of Modern Nursing, 2017Co-Authors: Xiukun Wang, Bing Wang, Tong Gao, Junxia Bai, Shuo Liu, Nan LiangAbstract:Objective To explore the clinical effect of using disposable blood transfusion apparatus as Eyewash equipment. Methods The clinical effect of Eyewash equipment that changed from disposable blood transfusion apparatus was compared with that of traditional Eyewash equipment. Results Compared with the traditional Eyewash equipment, the time of eye wash was decreased from five minutes to two minutes; the number of nurses responsible for washing every day was reduced from four to two; incidence rate of complications related to bacterial endophthalmitis was reduced from 0.10‰ to 0.05 ‰; error rate was dropped from 0.130‰ to 0.024‰; complaint rate was reduced from 0.087‰ to 0.012‰ (P<0.05) . Conclusions Application of disposable blood transfusion apparatus in eye washing is effect and safe, it can reduce the incidence of complications and improve the satisfaction of patients and medical staff. Key words: Eyewash equipment; Cataract surgery; Complications
Claudia Guzzoni Iudice - One of the best experts on this subject based on the ideXlab platform.
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three immobilized enzymes acting in series in layer by layer assemblies exploiting the trehalase glucose oxidase horseradish peroxidase cascade reactions for the optical determination of trehalose
Sensors and Actuators B-chemical, 2014Co-Authors: Gerardo Palazzo, Giuseppe Colafemmina, Claudia Guzzoni Iudice, Antonia MallardiAbstract:Abstract A new optical biosensor for trehalose determination has been realized immobilizing three glycoenzymes on a transparent support. Trehalase, glucose oxidase and horseradish peroxidase have been alternated with layers of Concanavalin A by a “layer-by-layer” (LbL) deposition. The driving force of this assembly is the biospecific complexation between Concanavalin A and sugar residues in the glycoenzymes. As confirmed by UV–vis spectroscopy, the LbL deposition allowed a high ordinate architecture with high loading of enzymes. After the assembly, the functionality of immobilized enzymes was spectrophotometrically proven, demonstrating also that they can act in series catalyzing cascade reactions. The prepared biosensor was used to optically detect trehalose, giving a LOD of 10 μM and a linear response up to 4 mM, and it showed also good time stability. The trehalose content in a real sample (Eyewash) was successfully determined by the biosensor.
