The Experts below are selected from a list of 102 Experts worldwide ranked by ideXlab platform
Jafar Ai - One of the best experts on this subject based on the ideXlab platform.
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enhancing neuronal growth from human endometrial stem cells derived neuron like cells in three dimensional fibrin gel for nerve tissue engineering
Journal of Biomedical Materials Research Part A, 2014Co-Authors: Mona Navaeinigjeh, Ghasem Amoabedini, Abbas Noroozi, Mahmoud Azami, Mohammad Nabi Asmani, Armin Ai, Somayeh Ebrahimibarough, Hooshang Saberi, Jafar AiAbstract:Nerve tissue engineering (NTE) is one of the most promising methods to restore central nerve systems in human health care. Three-dimensional (3D) distribution and growth of cells within the porous Scaffold composed of nanofibers are of clinical significance for NTE. In this study, an attempt was made to develop and characterize the use of fibrin gel and human endometrial stem cells (hEnSCs)-derived neuron-like cells simultaneously to support cell behavior especially neuron outgrowth. The structural and mechanical characteristics of fibrin gel Scaffold were examined with SEM and rheometer. Also, hEnSCs-derived neuron-like cells were cultured in fibrin gel and were subsequently analyzed with immunofluorescent staining against neuronal markers. In parallel, the survival and growth rates of the cells were determined by MTT assay and neurite extension. At the end, cell–matrix interactions were investigated with SEM and TEM micrographs. Mechanical properties of Fabricated Scaffold were studied and results indicated appropriate choice of material, SEM and TEM showed excellent integration of cells with nanofibers regarding the relation between cells and fibrin gel. Immunofluorescent staining of fibrin gel after 6 days of cell seeding and culture demonstrated well expanded and incorporated network of neurons. In addition, viability, proliferation, and neuronal growth of seeded cells were analyzed at days 1, 3, and 6. Comparing those results with 2D culture of seeded cells showed positive effect of 3D culture. Taken together, the results suggest that fibrin can provide a suitable, three-dimensional Scaffold for neuronal survival and outgrowth for regeneration of the central nervous system. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 2533–2543, 2014.
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Enhancing neuronal growth from human endometrial stem cells derived neuron‐like cells in three‐dimensional fibrin gel for nerve tissue engineering
Journal of Biomedical Materials Research Part A, 2013Co-Authors: Mona Navaei-nigjeh, Somayeh Ebrahimi-barough, Ghasem Amoabedini, Abbas Noroozi, Mahmoud Azami, Mohammad Nabi Asmani, Armin Ai, Hooshang Saberi, Jafar AiAbstract:Nerve tissue engineering (NTE) is one of the most promising methods to restore central nerve systems in human health care. Three-dimensional (3D) distribution and growth of cells within the porous Scaffold composed of nanofibers are of clinical significance for NTE. In this study, an attempt was made to develop and characterize the use of fibrin gel and human endometrial stem cells (hEnSCs)-derived neuron-like cells simultaneously to support cell behavior especially neuron outgrowth. The structural and mechanical characteristics of fibrin gel Scaffold were examined with SEM and rheometer. Also, hEnSCs-derived neuron-like cells were cultured in fibrin gel and were subsequently analyzed with immunofluorescent staining against neuronal markers. In parallel, the survival and growth rates of the cells were determined by MTT assay and neurite extension. At the end, cell–matrix interactions were investigated with SEM and TEM micrographs. Mechanical properties of Fabricated Scaffold were studied and results indicated appropriate choice of material, SEM and TEM showed excellent integration of cells with nanofibers regarding the relation between cells and fibrin gel. Immunofluorescent staining of fibrin gel after 6 days of cell seeding and culture demonstrated well expanded and incorporated network of neurons. In addition, viability, proliferation, and neuronal growth of seeded cells were analyzed at days 1, 3, and 6. Comparing those results with 2D culture of seeded cells showed positive effect of 3D culture. Taken together, the results suggest that fibrin can provide a suitable, three-dimensional Scaffold for neuronal survival and outgrowth for regeneration of the central nervous system. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 2533–2543, 2014.
Mona Navaeinigjeh - One of the best experts on this subject based on the ideXlab platform.
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enhancing neuronal growth from human endometrial stem cells derived neuron like cells in three dimensional fibrin gel for nerve tissue engineering
Journal of Biomedical Materials Research Part A, 2014Co-Authors: Mona Navaeinigjeh, Ghasem Amoabedini, Abbas Noroozi, Mahmoud Azami, Mohammad Nabi Asmani, Armin Ai, Somayeh Ebrahimibarough, Hooshang Saberi, Jafar AiAbstract:Nerve tissue engineering (NTE) is one of the most promising methods to restore central nerve systems in human health care. Three-dimensional (3D) distribution and growth of cells within the porous Scaffold composed of nanofibers are of clinical significance for NTE. In this study, an attempt was made to develop and characterize the use of fibrin gel and human endometrial stem cells (hEnSCs)-derived neuron-like cells simultaneously to support cell behavior especially neuron outgrowth. The structural and mechanical characteristics of fibrin gel Scaffold were examined with SEM and rheometer. Also, hEnSCs-derived neuron-like cells were cultured in fibrin gel and were subsequently analyzed with immunofluorescent staining against neuronal markers. In parallel, the survival and growth rates of the cells were determined by MTT assay and neurite extension. At the end, cell–matrix interactions were investigated with SEM and TEM micrographs. Mechanical properties of Fabricated Scaffold were studied and results indicated appropriate choice of material, SEM and TEM showed excellent integration of cells with nanofibers regarding the relation between cells and fibrin gel. Immunofluorescent staining of fibrin gel after 6 days of cell seeding and culture demonstrated well expanded and incorporated network of neurons. In addition, viability, proliferation, and neuronal growth of seeded cells were analyzed at days 1, 3, and 6. Comparing those results with 2D culture of seeded cells showed positive effect of 3D culture. Taken together, the results suggest that fibrin can provide a suitable, three-dimensional Scaffold for neuronal survival and outgrowth for regeneration of the central nervous system. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 2533–2543, 2014.
Hooshang Saberi - One of the best experts on this subject based on the ideXlab platform.
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enhancing neuronal growth from human endometrial stem cells derived neuron like cells in three dimensional fibrin gel for nerve tissue engineering
Journal of Biomedical Materials Research Part A, 2014Co-Authors: Mona Navaeinigjeh, Ghasem Amoabedini, Abbas Noroozi, Mahmoud Azami, Mohammad Nabi Asmani, Armin Ai, Somayeh Ebrahimibarough, Hooshang Saberi, Jafar AiAbstract:Nerve tissue engineering (NTE) is one of the most promising methods to restore central nerve systems in human health care. Three-dimensional (3D) distribution and growth of cells within the porous Scaffold composed of nanofibers are of clinical significance for NTE. In this study, an attempt was made to develop and characterize the use of fibrin gel and human endometrial stem cells (hEnSCs)-derived neuron-like cells simultaneously to support cell behavior especially neuron outgrowth. The structural and mechanical characteristics of fibrin gel Scaffold were examined with SEM and rheometer. Also, hEnSCs-derived neuron-like cells were cultured in fibrin gel and were subsequently analyzed with immunofluorescent staining against neuronal markers. In parallel, the survival and growth rates of the cells were determined by MTT assay and neurite extension. At the end, cell–matrix interactions were investigated with SEM and TEM micrographs. Mechanical properties of Fabricated Scaffold were studied and results indicated appropriate choice of material, SEM and TEM showed excellent integration of cells with nanofibers regarding the relation between cells and fibrin gel. Immunofluorescent staining of fibrin gel after 6 days of cell seeding and culture demonstrated well expanded and incorporated network of neurons. In addition, viability, proliferation, and neuronal growth of seeded cells were analyzed at days 1, 3, and 6. Comparing those results with 2D culture of seeded cells showed positive effect of 3D culture. Taken together, the results suggest that fibrin can provide a suitable, three-dimensional Scaffold for neuronal survival and outgrowth for regeneration of the central nervous system. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 2533–2543, 2014.
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Enhancing neuronal growth from human endometrial stem cells derived neuron‐like cells in three‐dimensional fibrin gel for nerve tissue engineering
Journal of Biomedical Materials Research Part A, 2013Co-Authors: Mona Navaei-nigjeh, Somayeh Ebrahimi-barough, Ghasem Amoabedini, Abbas Noroozi, Mahmoud Azami, Mohammad Nabi Asmani, Armin Ai, Hooshang Saberi, Jafar AiAbstract:Nerve tissue engineering (NTE) is one of the most promising methods to restore central nerve systems in human health care. Three-dimensional (3D) distribution and growth of cells within the porous Scaffold composed of nanofibers are of clinical significance for NTE. In this study, an attempt was made to develop and characterize the use of fibrin gel and human endometrial stem cells (hEnSCs)-derived neuron-like cells simultaneously to support cell behavior especially neuron outgrowth. The structural and mechanical characteristics of fibrin gel Scaffold were examined with SEM and rheometer. Also, hEnSCs-derived neuron-like cells were cultured in fibrin gel and were subsequently analyzed with immunofluorescent staining against neuronal markers. In parallel, the survival and growth rates of the cells were determined by MTT assay and neurite extension. At the end, cell–matrix interactions were investigated with SEM and TEM micrographs. Mechanical properties of Fabricated Scaffold were studied and results indicated appropriate choice of material, SEM and TEM showed excellent integration of cells with nanofibers regarding the relation between cells and fibrin gel. Immunofluorescent staining of fibrin gel after 6 days of cell seeding and culture demonstrated well expanded and incorporated network of neurons. In addition, viability, proliferation, and neuronal growth of seeded cells were analyzed at days 1, 3, and 6. Comparing those results with 2D culture of seeded cells showed positive effect of 3D culture. Taken together, the results suggest that fibrin can provide a suitable, three-dimensional Scaffold for neuronal survival and outgrowth for regeneration of the central nervous system. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 2533–2543, 2014.
Armin Ai - One of the best experts on this subject based on the ideXlab platform.
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enhancing neuronal growth from human endometrial stem cells derived neuron like cells in three dimensional fibrin gel for nerve tissue engineering
Journal of Biomedical Materials Research Part A, 2014Co-Authors: Mona Navaeinigjeh, Ghasem Amoabedini, Abbas Noroozi, Mahmoud Azami, Mohammad Nabi Asmani, Armin Ai, Somayeh Ebrahimibarough, Hooshang Saberi, Jafar AiAbstract:Nerve tissue engineering (NTE) is one of the most promising methods to restore central nerve systems in human health care. Three-dimensional (3D) distribution and growth of cells within the porous Scaffold composed of nanofibers are of clinical significance for NTE. In this study, an attempt was made to develop and characterize the use of fibrin gel and human endometrial stem cells (hEnSCs)-derived neuron-like cells simultaneously to support cell behavior especially neuron outgrowth. The structural and mechanical characteristics of fibrin gel Scaffold were examined with SEM and rheometer. Also, hEnSCs-derived neuron-like cells were cultured in fibrin gel and were subsequently analyzed with immunofluorescent staining against neuronal markers. In parallel, the survival and growth rates of the cells were determined by MTT assay and neurite extension. At the end, cell–matrix interactions were investigated with SEM and TEM micrographs. Mechanical properties of Fabricated Scaffold were studied and results indicated appropriate choice of material, SEM and TEM showed excellent integration of cells with nanofibers regarding the relation between cells and fibrin gel. Immunofluorescent staining of fibrin gel after 6 days of cell seeding and culture demonstrated well expanded and incorporated network of neurons. In addition, viability, proliferation, and neuronal growth of seeded cells were analyzed at days 1, 3, and 6. Comparing those results with 2D culture of seeded cells showed positive effect of 3D culture. Taken together, the results suggest that fibrin can provide a suitable, three-dimensional Scaffold for neuronal survival and outgrowth for regeneration of the central nervous system. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 2533–2543, 2014.
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Enhancing neuronal growth from human endometrial stem cells derived neuron‐like cells in three‐dimensional fibrin gel for nerve tissue engineering
Journal of Biomedical Materials Research Part A, 2013Co-Authors: Mona Navaei-nigjeh, Somayeh Ebrahimi-barough, Ghasem Amoabedini, Abbas Noroozi, Mahmoud Azami, Mohammad Nabi Asmani, Armin Ai, Hooshang Saberi, Jafar AiAbstract:Nerve tissue engineering (NTE) is one of the most promising methods to restore central nerve systems in human health care. Three-dimensional (3D) distribution and growth of cells within the porous Scaffold composed of nanofibers are of clinical significance for NTE. In this study, an attempt was made to develop and characterize the use of fibrin gel and human endometrial stem cells (hEnSCs)-derived neuron-like cells simultaneously to support cell behavior especially neuron outgrowth. The structural and mechanical characteristics of fibrin gel Scaffold were examined with SEM and rheometer. Also, hEnSCs-derived neuron-like cells were cultured in fibrin gel and were subsequently analyzed with immunofluorescent staining against neuronal markers. In parallel, the survival and growth rates of the cells were determined by MTT assay and neurite extension. At the end, cell–matrix interactions were investigated with SEM and TEM micrographs. Mechanical properties of Fabricated Scaffold were studied and results indicated appropriate choice of material, SEM and TEM showed excellent integration of cells with nanofibers regarding the relation between cells and fibrin gel. Immunofluorescent staining of fibrin gel after 6 days of cell seeding and culture demonstrated well expanded and incorporated network of neurons. In addition, viability, proliferation, and neuronal growth of seeded cells were analyzed at days 1, 3, and 6. Comparing those results with 2D culture of seeded cells showed positive effect of 3D culture. Taken together, the results suggest that fibrin can provide a suitable, three-dimensional Scaffold for neuronal survival and outgrowth for regeneration of the central nervous system. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 2533–2543, 2014.
Mahmoud Azami - One of the best experts on this subject based on the ideXlab platform.
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enhancing neuronal growth from human endometrial stem cells derived neuron like cells in three dimensional fibrin gel for nerve tissue engineering
Journal of Biomedical Materials Research Part A, 2014Co-Authors: Mona Navaeinigjeh, Ghasem Amoabedini, Abbas Noroozi, Mahmoud Azami, Mohammad Nabi Asmani, Armin Ai, Somayeh Ebrahimibarough, Hooshang Saberi, Jafar AiAbstract:Nerve tissue engineering (NTE) is one of the most promising methods to restore central nerve systems in human health care. Three-dimensional (3D) distribution and growth of cells within the porous Scaffold composed of nanofibers are of clinical significance for NTE. In this study, an attempt was made to develop and characterize the use of fibrin gel and human endometrial stem cells (hEnSCs)-derived neuron-like cells simultaneously to support cell behavior especially neuron outgrowth. The structural and mechanical characteristics of fibrin gel Scaffold were examined with SEM and rheometer. Also, hEnSCs-derived neuron-like cells were cultured in fibrin gel and were subsequently analyzed with immunofluorescent staining against neuronal markers. In parallel, the survival and growth rates of the cells were determined by MTT assay and neurite extension. At the end, cell–matrix interactions were investigated with SEM and TEM micrographs. Mechanical properties of Fabricated Scaffold were studied and results indicated appropriate choice of material, SEM and TEM showed excellent integration of cells with nanofibers regarding the relation between cells and fibrin gel. Immunofluorescent staining of fibrin gel after 6 days of cell seeding and culture demonstrated well expanded and incorporated network of neurons. In addition, viability, proliferation, and neuronal growth of seeded cells were analyzed at days 1, 3, and 6. Comparing those results with 2D culture of seeded cells showed positive effect of 3D culture. Taken together, the results suggest that fibrin can provide a suitable, three-dimensional Scaffold for neuronal survival and outgrowth for regeneration of the central nervous system. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 2533–2543, 2014.
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Enhancing neuronal growth from human endometrial stem cells derived neuron‐like cells in three‐dimensional fibrin gel for nerve tissue engineering
Journal of Biomedical Materials Research Part A, 2013Co-Authors: Mona Navaei-nigjeh, Somayeh Ebrahimi-barough, Ghasem Amoabedini, Abbas Noroozi, Mahmoud Azami, Mohammad Nabi Asmani, Armin Ai, Hooshang Saberi, Jafar AiAbstract:Nerve tissue engineering (NTE) is one of the most promising methods to restore central nerve systems in human health care. Three-dimensional (3D) distribution and growth of cells within the porous Scaffold composed of nanofibers are of clinical significance for NTE. In this study, an attempt was made to develop and characterize the use of fibrin gel and human endometrial stem cells (hEnSCs)-derived neuron-like cells simultaneously to support cell behavior especially neuron outgrowth. The structural and mechanical characteristics of fibrin gel Scaffold were examined with SEM and rheometer. Also, hEnSCs-derived neuron-like cells were cultured in fibrin gel and were subsequently analyzed with immunofluorescent staining against neuronal markers. In parallel, the survival and growth rates of the cells were determined by MTT assay and neurite extension. At the end, cell–matrix interactions were investigated with SEM and TEM micrographs. Mechanical properties of Fabricated Scaffold were studied and results indicated appropriate choice of material, SEM and TEM showed excellent integration of cells with nanofibers regarding the relation between cells and fibrin gel. Immunofluorescent staining of fibrin gel after 6 days of cell seeding and culture demonstrated well expanded and incorporated network of neurons. In addition, viability, proliferation, and neuronal growth of seeded cells were analyzed at days 1, 3, and 6. Comparing those results with 2D culture of seeded cells showed positive effect of 3D culture. Taken together, the results suggest that fibrin can provide a suitable, three-dimensional Scaffold for neuronal survival and outgrowth for regeneration of the central nervous system. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 2533–2543, 2014.
