The Experts below are selected from a list of 63 Experts worldwide ranked by ideXlab platform
Seiji Matsuda - One of the best experts on this subject based on the ideXlab platform.
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Expression patterns in alternative splicing forms of prosaposin mRNA in the rat Facial Nerve Nucleus after Facial Nerve transection
Neuroscience research, 2007Co-Authors: Jie Chen, Shouichiro Saito, Naoto Kobayashi, Kohji Sato, Katsumi Mominoki, Akira Sano, Tetsuya Shimokawa, Kyojy Miyawaki, Takehiro Terashita, Seiji MatsudaAbstract:Prosaposin acts as a neurotrophic factor, in addition to its role as the precursor protein for saposins A, B, C, and D, which are activators for specific sphingolipid hydrolases in lysosomes. In rats, the prosaposin gene generates two alternative splicing forms of mRNA: Pro+9 containing a 9-base insertion and Pro+0 without. The expression of these mRNAs changes after brain injury. We examined the expression patterns of the alternative splicing forms of prosaposin mRNA in the rat Facial Nerve Nucleus for 52 days following Facial Nerve transection. Pro+0 mRNA increased within 3 days of transection, peaked after 5-10 days, and remained significantly elevated for 21 days. In contrast, the expression of Pro+9 mRNA was constant throughout the regenerative period. Prosaposin mRNA expression increased not only in Facial motoneurons, but also in microglia during Facial Nerve regeneration. Our findings indicate that the saposin B domain of prosaposin, which is the domain affected by alternative splicing, plays an important role in both neurons and microglia during neuroregeneration.
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Changes in expression of prosaposin in the rat Facial Nerve Nucleus after Facial Nerve transection.
Neuroscience research, 2005Co-Authors: Kana Unuma, Hiroyuki Wakisaka, Shouichiro Saito, Jie Chen, Naoto Kobayashi, Kohji Sato, Kyoko Saito, Katsumi Mominoki, Akira Sano, Seiji MatsudaAbstract:Prosaposin is the precursor of saposins A, B, C and D, which are activators of sphingolipid hydrolases. In addition, unprocessed prosaposin functions as a neurotrophic factor in the central and peripheral nervous systems by acting to prevent neuronal apoptosis, to elongate neurites and to facilitate myelination. In this study, the expression pattern of prosaposin in the Facial Nerve Nucleus after Facial Nerve transection was examined by immunohistochemistry and in situ hybridization. Prosaposin immunoreactivity in the neurons on the operated side Facial Nerve Nucleus showed a biphasic pattern: it was significantly increased on day 3 after transection, decreased dramatically on day 7, started to increase gradually on day 14 and reached another peak on day 21 after transection. Significant increases in the levels of prosaposin mRNA were identified in the neurons on the operated side, suggesting that prosaposin was synthesized vigorously by the neurons themselves in the case of Facial Nerve transection. The diverse changes in prosaposin immunoreactivity during the process of Facial Nerve regeneration may reflect the diverse neurotrophic activities of prosaposin in Facial motoneurons.
Jie Chen - One of the best experts on this subject based on the ideXlab platform.
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Expression patterns in alternative splicing forms of prosaposin mRNA in the rat Facial Nerve Nucleus after Facial Nerve transection
Neuroscience research, 2007Co-Authors: Jie Chen, Shouichiro Saito, Naoto Kobayashi, Kohji Sato, Katsumi Mominoki, Akira Sano, Tetsuya Shimokawa, Kyojy Miyawaki, Takehiro Terashita, Seiji MatsudaAbstract:Prosaposin acts as a neurotrophic factor, in addition to its role as the precursor protein for saposins A, B, C, and D, which are activators for specific sphingolipid hydrolases in lysosomes. In rats, the prosaposin gene generates two alternative splicing forms of mRNA: Pro+9 containing a 9-base insertion and Pro+0 without. The expression of these mRNAs changes after brain injury. We examined the expression patterns of the alternative splicing forms of prosaposin mRNA in the rat Facial Nerve Nucleus for 52 days following Facial Nerve transection. Pro+0 mRNA increased within 3 days of transection, peaked after 5-10 days, and remained significantly elevated for 21 days. In contrast, the expression of Pro+9 mRNA was constant throughout the regenerative period. Prosaposin mRNA expression increased not only in Facial motoneurons, but also in microglia during Facial Nerve regeneration. Our findings indicate that the saposin B domain of prosaposin, which is the domain affected by alternative splicing, plays an important role in both neurons and microglia during neuroregeneration.
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Changes in expression of prosaposin in the rat Facial Nerve Nucleus after Facial Nerve transection.
Neuroscience research, 2005Co-Authors: Kana Unuma, Hiroyuki Wakisaka, Shouichiro Saito, Jie Chen, Naoto Kobayashi, Kohji Sato, Kyoko Saito, Katsumi Mominoki, Akira Sano, Seiji MatsudaAbstract:Prosaposin is the precursor of saposins A, B, C and D, which are activators of sphingolipid hydrolases. In addition, unprocessed prosaposin functions as a neurotrophic factor in the central and peripheral nervous systems by acting to prevent neuronal apoptosis, to elongate neurites and to facilitate myelination. In this study, the expression pattern of prosaposin in the Facial Nerve Nucleus after Facial Nerve transection was examined by immunohistochemistry and in situ hybridization. Prosaposin immunoreactivity in the neurons on the operated side Facial Nerve Nucleus showed a biphasic pattern: it was significantly increased on day 3 after transection, decreased dramatically on day 7, started to increase gradually on day 14 and reached another peak on day 21 after transection. Significant increases in the levels of prosaposin mRNA were identified in the neurons on the operated side, suggesting that prosaposin was synthesized vigorously by the neurons themselves in the case of Facial Nerve transection. The diverse changes in prosaposin immunoreactivity during the process of Facial Nerve regeneration may reflect the diverse neurotrophic activities of prosaposin in Facial motoneurons.
Naoto Kobayashi - One of the best experts on this subject based on the ideXlab platform.
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Expression patterns in alternative splicing forms of prosaposin mRNA in the rat Facial Nerve Nucleus after Facial Nerve transection
Neuroscience research, 2007Co-Authors: Jie Chen, Shouichiro Saito, Naoto Kobayashi, Kohji Sato, Katsumi Mominoki, Akira Sano, Tetsuya Shimokawa, Kyojy Miyawaki, Takehiro Terashita, Seiji MatsudaAbstract:Prosaposin acts as a neurotrophic factor, in addition to its role as the precursor protein for saposins A, B, C, and D, which are activators for specific sphingolipid hydrolases in lysosomes. In rats, the prosaposin gene generates two alternative splicing forms of mRNA: Pro+9 containing a 9-base insertion and Pro+0 without. The expression of these mRNAs changes after brain injury. We examined the expression patterns of the alternative splicing forms of prosaposin mRNA in the rat Facial Nerve Nucleus for 52 days following Facial Nerve transection. Pro+0 mRNA increased within 3 days of transection, peaked after 5-10 days, and remained significantly elevated for 21 days. In contrast, the expression of Pro+9 mRNA was constant throughout the regenerative period. Prosaposin mRNA expression increased not only in Facial motoneurons, but also in microglia during Facial Nerve regeneration. Our findings indicate that the saposin B domain of prosaposin, which is the domain affected by alternative splicing, plays an important role in both neurons and microglia during neuroregeneration.
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Changes in expression of prosaposin in the rat Facial Nerve Nucleus after Facial Nerve transection.
Neuroscience research, 2005Co-Authors: Kana Unuma, Hiroyuki Wakisaka, Shouichiro Saito, Jie Chen, Naoto Kobayashi, Kohji Sato, Kyoko Saito, Katsumi Mominoki, Akira Sano, Seiji MatsudaAbstract:Prosaposin is the precursor of saposins A, B, C and D, which are activators of sphingolipid hydrolases. In addition, unprocessed prosaposin functions as a neurotrophic factor in the central and peripheral nervous systems by acting to prevent neuronal apoptosis, to elongate neurites and to facilitate myelination. In this study, the expression pattern of prosaposin in the Facial Nerve Nucleus after Facial Nerve transection was examined by immunohistochemistry and in situ hybridization. Prosaposin immunoreactivity in the neurons on the operated side Facial Nerve Nucleus showed a biphasic pattern: it was significantly increased on day 3 after transection, decreased dramatically on day 7, started to increase gradually on day 14 and reached another peak on day 21 after transection. Significant increases in the levels of prosaposin mRNA were identified in the neurons on the operated side, suggesting that prosaposin was synthesized vigorously by the neurons themselves in the case of Facial Nerve transection. The diverse changes in prosaposin immunoreactivity during the process of Facial Nerve regeneration may reflect the diverse neurotrophic activities of prosaposin in Facial motoneurons.
Akira Sano - One of the best experts on this subject based on the ideXlab platform.
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Expression patterns in alternative splicing forms of prosaposin mRNA in the rat Facial Nerve Nucleus after Facial Nerve transection
Neuroscience research, 2007Co-Authors: Jie Chen, Shouichiro Saito, Naoto Kobayashi, Kohji Sato, Katsumi Mominoki, Akira Sano, Tetsuya Shimokawa, Kyojy Miyawaki, Takehiro Terashita, Seiji MatsudaAbstract:Prosaposin acts as a neurotrophic factor, in addition to its role as the precursor protein for saposins A, B, C, and D, which are activators for specific sphingolipid hydrolases in lysosomes. In rats, the prosaposin gene generates two alternative splicing forms of mRNA: Pro+9 containing a 9-base insertion and Pro+0 without. The expression of these mRNAs changes after brain injury. We examined the expression patterns of the alternative splicing forms of prosaposin mRNA in the rat Facial Nerve Nucleus for 52 days following Facial Nerve transection. Pro+0 mRNA increased within 3 days of transection, peaked after 5-10 days, and remained significantly elevated for 21 days. In contrast, the expression of Pro+9 mRNA was constant throughout the regenerative period. Prosaposin mRNA expression increased not only in Facial motoneurons, but also in microglia during Facial Nerve regeneration. Our findings indicate that the saposin B domain of prosaposin, which is the domain affected by alternative splicing, plays an important role in both neurons and microglia during neuroregeneration.
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Changes in expression of prosaposin in the rat Facial Nerve Nucleus after Facial Nerve transection.
Neuroscience research, 2005Co-Authors: Kana Unuma, Hiroyuki Wakisaka, Shouichiro Saito, Jie Chen, Naoto Kobayashi, Kohji Sato, Kyoko Saito, Katsumi Mominoki, Akira Sano, Seiji MatsudaAbstract:Prosaposin is the precursor of saposins A, B, C and D, which are activators of sphingolipid hydrolases. In addition, unprocessed prosaposin functions as a neurotrophic factor in the central and peripheral nervous systems by acting to prevent neuronal apoptosis, to elongate neurites and to facilitate myelination. In this study, the expression pattern of prosaposin in the Facial Nerve Nucleus after Facial Nerve transection was examined by immunohistochemistry and in situ hybridization. Prosaposin immunoreactivity in the neurons on the operated side Facial Nerve Nucleus showed a biphasic pattern: it was significantly increased on day 3 after transection, decreased dramatically on day 7, started to increase gradually on day 14 and reached another peak on day 21 after transection. Significant increases in the levels of prosaposin mRNA were identified in the neurons on the operated side, suggesting that prosaposin was synthesized vigorously by the neurons themselves in the case of Facial Nerve transection. The diverse changes in prosaposin immunoreactivity during the process of Facial Nerve regeneration may reflect the diverse neurotrophic activities of prosaposin in Facial motoneurons.
Katsumi Mominoki - One of the best experts on this subject based on the ideXlab platform.
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Expression patterns in alternative splicing forms of prosaposin mRNA in the rat Facial Nerve Nucleus after Facial Nerve transection
Neuroscience research, 2007Co-Authors: Jie Chen, Shouichiro Saito, Naoto Kobayashi, Kohji Sato, Katsumi Mominoki, Akira Sano, Tetsuya Shimokawa, Kyojy Miyawaki, Takehiro Terashita, Seiji MatsudaAbstract:Prosaposin acts as a neurotrophic factor, in addition to its role as the precursor protein for saposins A, B, C, and D, which are activators for specific sphingolipid hydrolases in lysosomes. In rats, the prosaposin gene generates two alternative splicing forms of mRNA: Pro+9 containing a 9-base insertion and Pro+0 without. The expression of these mRNAs changes after brain injury. We examined the expression patterns of the alternative splicing forms of prosaposin mRNA in the rat Facial Nerve Nucleus for 52 days following Facial Nerve transection. Pro+0 mRNA increased within 3 days of transection, peaked after 5-10 days, and remained significantly elevated for 21 days. In contrast, the expression of Pro+9 mRNA was constant throughout the regenerative period. Prosaposin mRNA expression increased not only in Facial motoneurons, but also in microglia during Facial Nerve regeneration. Our findings indicate that the saposin B domain of prosaposin, which is the domain affected by alternative splicing, plays an important role in both neurons and microglia during neuroregeneration.
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Changes in expression of prosaposin in the rat Facial Nerve Nucleus after Facial Nerve transection.
Neuroscience research, 2005Co-Authors: Kana Unuma, Hiroyuki Wakisaka, Shouichiro Saito, Jie Chen, Naoto Kobayashi, Kohji Sato, Kyoko Saito, Katsumi Mominoki, Akira Sano, Seiji MatsudaAbstract:Prosaposin is the precursor of saposins A, B, C and D, which are activators of sphingolipid hydrolases. In addition, unprocessed prosaposin functions as a neurotrophic factor in the central and peripheral nervous systems by acting to prevent neuronal apoptosis, to elongate neurites and to facilitate myelination. In this study, the expression pattern of prosaposin in the Facial Nerve Nucleus after Facial Nerve transection was examined by immunohistochemistry and in situ hybridization. Prosaposin immunoreactivity in the neurons on the operated side Facial Nerve Nucleus showed a biphasic pattern: it was significantly increased on day 3 after transection, decreased dramatically on day 7, started to increase gradually on day 14 and reached another peak on day 21 after transection. Significant increases in the levels of prosaposin mRNA were identified in the neurons on the operated side, suggesting that prosaposin was synthesized vigorously by the neurons themselves in the case of Facial Nerve transection. The diverse changes in prosaposin immunoreactivity during the process of Facial Nerve regeneration may reflect the diverse neurotrophic activities of prosaposin in Facial motoneurons.
