The Experts below are selected from a list of 40170 Experts worldwide ranked by ideXlab platform
Robert A. Holt - One of the best experts on this subject based on the ideXlab platform.
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High-throughput sequencing: a Failure Mode Analysis.
BMC genomics, 2005Co-Authors: George S. Yang, Jeffery M. Stott, Duane E. Smailus, Sarah Barber, Miruna Balasundaram, Marco A. Marra, Robert A. HoltAbstract:Basic manufacturing principles are becoming increasingly important in high-throughput sequencing facilities where there is a constant drive to increase quality, increase efficiency, and decrease operating costs. While high-throughput centres report Failure rates typically on the order of 10%, the causes of sporadic sequencing Failures are seldom analyzed in detail and have not, in the past, been formally reported. Here we report the results of a Failure Mode Analysis of our production sequencing facility based on detailed evaluation of 9,216 ESTs generated from two cDNA libraries. Two categories of Failures are described; process-related Failures (Failures due to equipment or sample handling) and template-related Failures (Failures that are revealed by close inspection of electropherograms and are likely due to properties of the template DNA sequence itself). Preventative action based on a detailed understanding of Failure Modes is likely to improve the performance of other production sequencing pipelines.
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High-throughput sequencing: a Failure Mode Analysis
BMC Genomics, 2005Co-Authors: George S. Yang, Jeffery M. Stott, Duane E. Smailus, Miruna Balasundaram, Marco A. Marra, Sarah A Barber, Robert A. HoltAbstract:Background Basic manufacturing principles are becoming increasingly important in high-throughput sequencing facilities where there is a constant drive to increase quality, increase efficiency, and decrease operating costs. While high-throughput centres report Failure rates typically on the order of 10%, the causes of sporadic sequencing Failures are seldom analyzed in detail and have not, in the past, been formally reported. Results Here we report the results of a Failure Mode Analysis of our production sequencing facility based on detailed evaluation of 9,216 ESTs generated from two cDNA libraries. Two categories of Failures are described; process-related Failures (Failures due to equipment or sample handling) and template-related Failures (Failures that are revealed by close inspection of electropherograms and are likely due to properties of the template DNA sequence itself). Conclusions Preventative action based on a detailed understanding of Failure Modes is likely to improve the performance of other production sequencing pipelines.
George S. Yang - One of the best experts on this subject based on the ideXlab platform.
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High-throughput sequencing: a Failure Mode Analysis.
BMC genomics, 2005Co-Authors: George S. Yang, Jeffery M. Stott, Duane E. Smailus, Sarah Barber, Miruna Balasundaram, Marco A. Marra, Robert A. HoltAbstract:Basic manufacturing principles are becoming increasingly important in high-throughput sequencing facilities where there is a constant drive to increase quality, increase efficiency, and decrease operating costs. While high-throughput centres report Failure rates typically on the order of 10%, the causes of sporadic sequencing Failures are seldom analyzed in detail and have not, in the past, been formally reported. Here we report the results of a Failure Mode Analysis of our production sequencing facility based on detailed evaluation of 9,216 ESTs generated from two cDNA libraries. Two categories of Failures are described; process-related Failures (Failures due to equipment or sample handling) and template-related Failures (Failures that are revealed by close inspection of electropherograms and are likely due to properties of the template DNA sequence itself). Preventative action based on a detailed understanding of Failure Modes is likely to improve the performance of other production sequencing pipelines.
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High-throughput sequencing: a Failure Mode Analysis
BMC Genomics, 2005Co-Authors: George S. Yang, Jeffery M. Stott, Duane E. Smailus, Miruna Balasundaram, Marco A. Marra, Sarah A Barber, Robert A. HoltAbstract:Background Basic manufacturing principles are becoming increasingly important in high-throughput sequencing facilities where there is a constant drive to increase quality, increase efficiency, and decrease operating costs. While high-throughput centres report Failure rates typically on the order of 10%, the causes of sporadic sequencing Failures are seldom analyzed in detail and have not, in the past, been formally reported. Results Here we report the results of a Failure Mode Analysis of our production sequencing facility based on detailed evaluation of 9,216 ESTs generated from two cDNA libraries. Two categories of Failures are described; process-related Failures (Failures due to equipment or sample handling) and template-related Failures (Failures that are revealed by close inspection of electropherograms and are likely due to properties of the template DNA sequence itself). Conclusions Preventative action based on a detailed understanding of Failure Modes is likely to improve the performance of other production sequencing pipelines.
Basudev Prasad - One of the best experts on this subject based on the ideXlab platform.
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Failure Mode Analysis of PV Modules in Different Climatic Conditions
IEEE Journal of Photovoltaics, 2021Co-Authors: Birinchi Bora, O.s. Sastry, Rajesh Kumar, Rajiv Dubey, Shashwata Chattopadhyay, Juzer Vasi, Som Mondal, Basudev PrasadAbstract:Understanding the reliability of photovoltaic (PV) modules under field conditions is one of the primary areas to ensure prolonged operation. This article reported a study on assessing PV modules' reliability under different outdoor environments, using procedures available in the literature. We identified dominant Failure Modes of PV modules under different climatic conditions of India. Risk priority number (RPN) of different Failure Modes are estimated by analyzing the consequences of defects in performance and safety. Modules deployed under cold and sunny, hot and dry, warm and humid, and composite climates of India have more varieties of Failure Modes than those in cold and cloudy and Moderate climatic conditions. The frequency of occurrences of benign or cosmetic defects of PV modules varies under different climatic zones. The biggest concerns in old PV systems were hot spots, internal circuitry discoloration, backsheet problems, and grounding wires' corrosion. In the hot zones, primary safety issues were frame grounding corrosion, backsheet problems, hot spots, and nonhot zones were backsheet burn marks, backsheet peeling, and grounding wire corrosion. The defects observed in different climatic zones were analyzed in terms of its possible defect generation route also.
Marco A. Marra - One of the best experts on this subject based on the ideXlab platform.
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High-throughput sequencing: a Failure Mode Analysis.
BMC genomics, 2005Co-Authors: George S. Yang, Jeffery M. Stott, Duane E. Smailus, Sarah Barber, Miruna Balasundaram, Marco A. Marra, Robert A. HoltAbstract:Basic manufacturing principles are becoming increasingly important in high-throughput sequencing facilities where there is a constant drive to increase quality, increase efficiency, and decrease operating costs. While high-throughput centres report Failure rates typically on the order of 10%, the causes of sporadic sequencing Failures are seldom analyzed in detail and have not, in the past, been formally reported. Here we report the results of a Failure Mode Analysis of our production sequencing facility based on detailed evaluation of 9,216 ESTs generated from two cDNA libraries. Two categories of Failures are described; process-related Failures (Failures due to equipment or sample handling) and template-related Failures (Failures that are revealed by close inspection of electropherograms and are likely due to properties of the template DNA sequence itself). Preventative action based on a detailed understanding of Failure Modes is likely to improve the performance of other production sequencing pipelines.
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High-throughput sequencing: a Failure Mode Analysis
BMC Genomics, 2005Co-Authors: George S. Yang, Jeffery M. Stott, Duane E. Smailus, Miruna Balasundaram, Marco A. Marra, Sarah A Barber, Robert A. HoltAbstract:Background Basic manufacturing principles are becoming increasingly important in high-throughput sequencing facilities where there is a constant drive to increase quality, increase efficiency, and decrease operating costs. While high-throughput centres report Failure rates typically on the order of 10%, the causes of sporadic sequencing Failures are seldom analyzed in detail and have not, in the past, been formally reported. Results Here we report the results of a Failure Mode Analysis of our production sequencing facility based on detailed evaluation of 9,216 ESTs generated from two cDNA libraries. Two categories of Failures are described; process-related Failures (Failures due to equipment or sample handling) and template-related Failures (Failures that are revealed by close inspection of electropherograms and are likely due to properties of the template DNA sequence itself). Conclusions Preventative action based on a detailed understanding of Failure Modes is likely to improve the performance of other production sequencing pipelines.
Jeffery M. Stott - One of the best experts on this subject based on the ideXlab platform.
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High-throughput sequencing: a Failure Mode Analysis.
BMC genomics, 2005Co-Authors: George S. Yang, Jeffery M. Stott, Duane E. Smailus, Sarah Barber, Miruna Balasundaram, Marco A. Marra, Robert A. HoltAbstract:Basic manufacturing principles are becoming increasingly important in high-throughput sequencing facilities where there is a constant drive to increase quality, increase efficiency, and decrease operating costs. While high-throughput centres report Failure rates typically on the order of 10%, the causes of sporadic sequencing Failures are seldom analyzed in detail and have not, in the past, been formally reported. Here we report the results of a Failure Mode Analysis of our production sequencing facility based on detailed evaluation of 9,216 ESTs generated from two cDNA libraries. Two categories of Failures are described; process-related Failures (Failures due to equipment or sample handling) and template-related Failures (Failures that are revealed by close inspection of electropherograms and are likely due to properties of the template DNA sequence itself). Preventative action based on a detailed understanding of Failure Modes is likely to improve the performance of other production sequencing pipelines.
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High-throughput sequencing: a Failure Mode Analysis
BMC Genomics, 2005Co-Authors: George S. Yang, Jeffery M. Stott, Duane E. Smailus, Miruna Balasundaram, Marco A. Marra, Sarah A Barber, Robert A. HoltAbstract:Background Basic manufacturing principles are becoming increasingly important in high-throughput sequencing facilities where there is a constant drive to increase quality, increase efficiency, and decrease operating costs. While high-throughput centres report Failure rates typically on the order of 10%, the causes of sporadic sequencing Failures are seldom analyzed in detail and have not, in the past, been formally reported. Results Here we report the results of a Failure Mode Analysis of our production sequencing facility based on detailed evaluation of 9,216 ESTs generated from two cDNA libraries. Two categories of Failures are described; process-related Failures (Failures due to equipment or sample handling) and template-related Failures (Failures that are revealed by close inspection of electropherograms and are likely due to properties of the template DNA sequence itself). Conclusions Preventative action based on a detailed understanding of Failure Modes is likely to improve the performance of other production sequencing pipelines.
