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Yuuhiko Tanabe - One of the best experts on this subject based on the ideXlab platform.

  • erratum to the Fast Growing strain of hydrocarbon rich green alga botryococcus braunii bot 22 is a vitamin b12 autotroph
    Journal of Applied Phycology, 2014
    Co-Authors: Yuuhiko Tanabe, Motohide Ioki, Makoto Watanabe
    Abstract:

    Botryococcus braunii is one of the most promising microalgal feedstocks for biofuel production because it accumulates a large amount of hydrocarbons within the colony. Several researchers have used vitamin-free media for short-term culture experiments of B. braunii. However, vitamin requirements of B. braunii have never been explicitly described. Therefore, the consequences of long-term vitamin deficiency remain unclear. To investigate the vitamin B12 (cobalamin) requirement of B. braunii, the growth characteristics and oil productivity were compared using an axenic strain B. braunii BOT-22, one of the Fast-Growing Strains, with and without vitamin B12 supplementation. The growth rate, maximum biomass concentration, and lipid content did not differ between cultures with and without vitamin B12 supplements. In addition, an in silico survey of the transcriptomic dataset of B. braunii BOT-22 identified a putative gene fragment coding for the vitamin B12-independent methionine synthetase (metE), which is a hallmark of the vitamin B12 autotrophy in algae. In conclusion, these data suggest that B. braunii BOT-22 is a vitamin B12 autotroph.

  • The Fast-Growing strain of hydrocarbon-rich green alga Botryococcus braunii, BOT-22, is a vitamin B12 autotroph.
    Journal of Applied Phycology, 2013
    Co-Authors: Yuuhiko Tanabe, Motohide Ioki, Makoto M Watanabe
    Abstract:

    Botryococcus braunii is one of the most promising microalgal feedstocks for biofuel production because it accumulates a large amount of hydrocarbons within the colony. Several researchers have used vitamin-free media for short-term culture experiments of B. braunii. However, vitamin requirements of B. braunii have never been explicitly described. Therefore, the consequences of long-term vitamin deficiency remain unclear. To investigate the vitamin B12 (cobalamin) requirement of B. braunii, the growth characteristics and oil productivity were compared using an axenic strain B. braunii BOT-22, one of the Fast-Growing Strains, with and without vitamin B12 supplementation. The growth rate, maximum biomass concentration, and lipid content did not differ between cultures with and without vitamin B12 supplements. In addition, an in silico survey of the transcriptomic dataset of B. braunii BOT-22 identified a putative gene fragment coding for the vitamin B12-independent methionine synthetase (metE), which is a hallmark of the vitamin B12 autotrophy in algae. In conclusion, these data suggest that B. braunii BOT-22 is a vitamin B12 autotroph.

Makoto Watanabe - One of the best experts on this subject based on the ideXlab platform.

  • erratum to the Fast Growing strain of hydrocarbon rich green alga botryococcus braunii bot 22 is a vitamin b12 autotroph
    Journal of Applied Phycology, 2014
    Co-Authors: Yuuhiko Tanabe, Motohide Ioki, Makoto Watanabe
    Abstract:

    Botryococcus braunii is one of the most promising microalgal feedstocks for biofuel production because it accumulates a large amount of hydrocarbons within the colony. Several researchers have used vitamin-free media for short-term culture experiments of B. braunii. However, vitamin requirements of B. braunii have never been explicitly described. Therefore, the consequences of long-term vitamin deficiency remain unclear. To investigate the vitamin B12 (cobalamin) requirement of B. braunii, the growth characteristics and oil productivity were compared using an axenic strain B. braunii BOT-22, one of the Fast-Growing Strains, with and without vitamin B12 supplementation. The growth rate, maximum biomass concentration, and lipid content did not differ between cultures with and without vitamin B12 supplements. In addition, an in silico survey of the transcriptomic dataset of B. braunii BOT-22 identified a putative gene fragment coding for the vitamin B12-independent methionine synthetase (metE), which is a hallmark of the vitamin B12 autotrophy in algae. In conclusion, these data suggest that B. braunii BOT-22 is a vitamin B12 autotroph.

Makoto M Watanabe - One of the best experts on this subject based on the ideXlab platform.

  • The Fast-Growing strain of hydrocarbon-rich green alga Botryococcus braunii, BOT-22, is a vitamin B12 autotroph.
    Journal of Applied Phycology, 2013
    Co-Authors: Yuuhiko Tanabe, Motohide Ioki, Makoto M Watanabe
    Abstract:

    Botryococcus braunii is one of the most promising microalgal feedstocks for biofuel production because it accumulates a large amount of hydrocarbons within the colony. Several researchers have used vitamin-free media for short-term culture experiments of B. braunii. However, vitamin requirements of B. braunii have never been explicitly described. Therefore, the consequences of long-term vitamin deficiency remain unclear. To investigate the vitamin B12 (cobalamin) requirement of B. braunii, the growth characteristics and oil productivity were compared using an axenic strain B. braunii BOT-22, one of the Fast-Growing Strains, with and without vitamin B12 supplementation. The growth rate, maximum biomass concentration, and lipid content did not differ between cultures with and without vitamin B12 supplements. In addition, an in silico survey of the transcriptomic dataset of B. braunii BOT-22 identified a putative gene fragment coding for the vitamin B12-independent methionine synthetase (metE), which is a hallmark of the vitamin B12 autotrophy in algae. In conclusion, these data suggest that B. braunii BOT-22 is a vitamin B12 autotroph.

Motohide Ioki - One of the best experts on this subject based on the ideXlab platform.

  • erratum to the Fast Growing strain of hydrocarbon rich green alga botryococcus braunii bot 22 is a vitamin b12 autotroph
    Journal of Applied Phycology, 2014
    Co-Authors: Yuuhiko Tanabe, Motohide Ioki, Makoto Watanabe
    Abstract:

    Botryococcus braunii is one of the most promising microalgal feedstocks for biofuel production because it accumulates a large amount of hydrocarbons within the colony. Several researchers have used vitamin-free media for short-term culture experiments of B. braunii. However, vitamin requirements of B. braunii have never been explicitly described. Therefore, the consequences of long-term vitamin deficiency remain unclear. To investigate the vitamin B12 (cobalamin) requirement of B. braunii, the growth characteristics and oil productivity were compared using an axenic strain B. braunii BOT-22, one of the Fast-Growing Strains, with and without vitamin B12 supplementation. The growth rate, maximum biomass concentration, and lipid content did not differ between cultures with and without vitamin B12 supplements. In addition, an in silico survey of the transcriptomic dataset of B. braunii BOT-22 identified a putative gene fragment coding for the vitamin B12-independent methionine synthetase (metE), which is a hallmark of the vitamin B12 autotrophy in algae. In conclusion, these data suggest that B. braunii BOT-22 is a vitamin B12 autotroph.

  • The Fast-Growing strain of hydrocarbon-rich green alga Botryococcus braunii, BOT-22, is a vitamin B12 autotroph.
    Journal of Applied Phycology, 2013
    Co-Authors: Yuuhiko Tanabe, Motohide Ioki, Makoto M Watanabe
    Abstract:

    Botryococcus braunii is one of the most promising microalgal feedstocks for biofuel production because it accumulates a large amount of hydrocarbons within the colony. Several researchers have used vitamin-free media for short-term culture experiments of B. braunii. However, vitamin requirements of B. braunii have never been explicitly described. Therefore, the consequences of long-term vitamin deficiency remain unclear. To investigate the vitamin B12 (cobalamin) requirement of B. braunii, the growth characteristics and oil productivity were compared using an axenic strain B. braunii BOT-22, one of the Fast-Growing Strains, with and without vitamin B12 supplementation. The growth rate, maximum biomass concentration, and lipid content did not differ between cultures with and without vitamin B12 supplements. In addition, an in silico survey of the transcriptomic dataset of B. braunii BOT-22 identified a putative gene fragment coding for the vitamin B12-independent methionine synthetase (metE), which is a hallmark of the vitamin B12 autotrophy in algae. In conclusion, these data suggest that B. braunii BOT-22 is a vitamin B12 autotroph.

Hugues De Verdal - One of the best experts on this subject based on the ideXlab platform.

  • Mitochondrial Genetic Diversity among Farmed Stocks of Oreochromis spp. (Perciformes, Cichlidae) in Madagascar
    Diversity, 2021
    Co-Authors: Nicolas Hubert, Elodie Pepey, Jean-michel Mortillaro, Dirk Steinke, Diana Edithe Andria-mananjara, Hugues De Verdal
    Abstract:

    The Fast development of aquaculture over the past decades has made it the main source of fish protein and led to its integration into the global food system. Mostly originating from inland production systems, aquaculture has emerged as strategy to decrease malnutrition in low-income countries. The Nile tilapia (Oreochromis niloticus) was introduced to Madagascar in the 1950s, and is now produced nationally at various scales. Aquaculture mostly relies on fry harvested from wild populations and grow-out in ponds for decades. It has recently been diversified by the introduction of several Fast-Growing Strains. Little is known how local genetic diversity compares to recently introduced Strains, although high and comparable levels of genetic diversity have previously been observed for both wild populations and local stocks. Our study compares DNA barcode genetic diversity among eight farms and several Strains belonging to three species sampled. DNA-based lineage delimitation methods were applied and resulted in the detection of six well differentiated and highly divergent lineages. A comparison of DNA barcode records to sequences on the Barcode of Life Data System (BOLD) helped to trace the origin of several of them. Both haplotype and nucleotide diversity indices highlight high levels of mitochondrial genetic diversity, with several local Strains displaying higher diversity than recently introduced Strains. This allows for multiple options to maintain high levels of genetic diversity in broodstock and provides more options for selective breeding programs.