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Even Heir - One of the best experts on this subject based on the ideXlab platform.
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The persistence of Salmonella following desiccation under Feed Processing environmental conditions: a subject of relevance.
Letters in Applied Microbiology, 2014Co-Authors: Olivier Habimana, Trond Moretro, Lene K Vestby, Live L Nesse, Even Heir, Kristin Berg, Solveig LangsrudAbstract:UNLABELLED Although Salmonella persistence has been predominantly linked to biofilm formation, the physiological state of Salmonella should also be considered as a possible pathway for persistence and survival in the Feed industry. Hence, the purpose of this study was to assess the extent of viability of Salmonella cells through long-term desiccation periods under conditions typically found in Feed Processing environments, and whether these same cells could resuscitate and cause salmonellosis in vivo. We showed that upon desiccation, Salmonella Agona, a representative Feed industry isolate and Salmonella Typhimurium ATCC 14028, a laboratory strain, were induced into a nonculturable state at 35 and 85% relative humidity conditions, at defined temperatures of 30 and 12°C, respectively. Although the reduction in culturable cells was more than 6 log10 , metabolic activity was found in more than 1% of the population. Desiccation-induced nonculturable Salm. Typhimurium could not be revived and were nonvirulent in a mouse model following infection through oral gavage. These results suggest that the specific conditions for reviving nonculturable Salmonella after long periods of desiccation are yet to be fully identified. The need for mapping key factors involved in the persistence of Salmonella would help better detect it and improve Feed safety measures. SIGNIFICANCE AND IMPACT OF THE STUDY While Salmonella has been shown to persist for years in Feed Processing environments, it is still unknown how temperature and humidity affect the persistence of Salmonella cells over time in terms of their metabolic states and cultivability. Here, we show that long-term exposure to Feed Processing environmental conditions induces Salmonella into a nonculturable state even though about 1% of the population remains metabolically active. This has significant implications when monitoring Salmonella from the environment which could yield false-negative results using conventional pre-enrichment detection methods.
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Micro ecosystems from Feed industry surfaces: a survival and biofilm study of Salmonellaversus host resident flora strains
BMC Veterinary Research, 2010Co-Authors: Olivier Habimana, Trond Moretro, Lene K Vestby, Live L Nesse, Solveig Langsrud, Even HeirAbstract:Background The presence of Salmonella enterica serovars in Feed ingredients, products and Processing facilities is a well recognized problem worldwide. In Norwegian Feed factories, strict control measures are implemented to avoid establishment and spreading of Salmonella throughout the Processing chain. There is limited knowledge on the presence and survival of the resident microflora in Feed production plants. Information on interactions between Salmonella and other bacteria in Feed production plants and how they affect survival and biofilm formation of Salmonella is also limited. The aim of this study was to identify resident microbiota found in Feed production environments, and to compare the survival of resident flora strains and Salmonella to stress factors typically found in Feed Processing environments. Moreover, the role of dominant resident flora strains in the biofilm development of Salmonella was determined. Results Surface microflora characterization from two Feed productions plants, by means of 16 S rDNA sequencing, revealed a wide diversity of bacteria. Survival, disinfection and biofilm formation experiments were conducted on selected dominant resident flora strains and Salmonella . Results showed higher survival properties by resident flora isolates for desiccation, and disinfection compared to Salmonella isolates. Dual-species biofilms favored Salmonella growth compared to Salmonella in mono-species biofilms, with biovolume increases of 2.8-fold and 3.2-fold in the presence of Staphylococcus and Pseudomonas , respectively. Conclusions These results offer an overview of the microflora composition found in Feed industry Processing environments, their survival under relevant stresses and their potential effect on biofilm formation in the presence of Salmonella . Eliminating the establishment of resident flora isolates in Feed industry surfaces is therefore of interest for impeding conditions for Salmonella colonization and growth on Feed industry surfaces. In-depth investigations are still needed to determine whether resident flora has a definite role in the persistence of Salmonella in Feed Processing environments.
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micro ecosystems from Feed industry surfaces a survival and biofilm study of salmonella versus host resident flora strains
BMC Veterinary Research, 2010Co-Authors: Olivier Habimana, Trond Moretro, Lene K Vestby, Live L Nesse, Solveig Langsrud, Even HeirAbstract:Background The presence of Salmonella enterica serovars in Feed ingredients, products and Processing facilities is a well recognized problem worldwide. In Norwegian Feed factories, strict control measures are implemented to avoid establishment and spreading of Salmonella throughout the Processing chain. There is limited knowledge on the presence and survival of the resident microflora in Feed production plants. Information on interactions between Salmonella and other bacteria in Feed production plants and how they affect survival and biofilm formation of Salmonella is also limited. The aim of this study was to identify resident microbiota found in Feed production environments, and to compare the survival of resident flora strains and Salmonella to stress factors typically found in Feed Processing environments. Moreover, the role of dominant resident flora strains in the biofilm development of Salmonella was determined.
Olivier Habimana - One of the best experts on this subject based on the ideXlab platform.
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The persistence of Salmonella following desiccation under Feed Processing environmental conditions: a subject of relevance.
Letters in Applied Microbiology, 2014Co-Authors: Olivier Habimana, Trond Moretro, Lene K Vestby, Live L Nesse, Even Heir, Kristin Berg, Solveig LangsrudAbstract:UNLABELLED Although Salmonella persistence has been predominantly linked to biofilm formation, the physiological state of Salmonella should also be considered as a possible pathway for persistence and survival in the Feed industry. Hence, the purpose of this study was to assess the extent of viability of Salmonella cells through long-term desiccation periods under conditions typically found in Feed Processing environments, and whether these same cells could resuscitate and cause salmonellosis in vivo. We showed that upon desiccation, Salmonella Agona, a representative Feed industry isolate and Salmonella Typhimurium ATCC 14028, a laboratory strain, were induced into a nonculturable state at 35 and 85% relative humidity conditions, at defined temperatures of 30 and 12°C, respectively. Although the reduction in culturable cells was more than 6 log10 , metabolic activity was found in more than 1% of the population. Desiccation-induced nonculturable Salm. Typhimurium could not be revived and were nonvirulent in a mouse model following infection through oral gavage. These results suggest that the specific conditions for reviving nonculturable Salmonella after long periods of desiccation are yet to be fully identified. The need for mapping key factors involved in the persistence of Salmonella would help better detect it and improve Feed safety measures. SIGNIFICANCE AND IMPACT OF THE STUDY While Salmonella has been shown to persist for years in Feed Processing environments, it is still unknown how temperature and humidity affect the persistence of Salmonella cells over time in terms of their metabolic states and cultivability. Here, we show that long-term exposure to Feed Processing environmental conditions induces Salmonella into a nonculturable state even though about 1% of the population remains metabolically active. This has significant implications when monitoring Salmonella from the environment which could yield false-negative results using conventional pre-enrichment detection methods.
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Micro ecosystems from Feed industry surfaces: a survival and biofilm study of Salmonellaversus host resident flora strains
BMC Veterinary Research, 2010Co-Authors: Olivier Habimana, Trond Moretro, Lene K Vestby, Live L Nesse, Solveig Langsrud, Even HeirAbstract:Background The presence of Salmonella enterica serovars in Feed ingredients, products and Processing facilities is a well recognized problem worldwide. In Norwegian Feed factories, strict control measures are implemented to avoid establishment and spreading of Salmonella throughout the Processing chain. There is limited knowledge on the presence and survival of the resident microflora in Feed production plants. Information on interactions between Salmonella and other bacteria in Feed production plants and how they affect survival and biofilm formation of Salmonella is also limited. The aim of this study was to identify resident microbiota found in Feed production environments, and to compare the survival of resident flora strains and Salmonella to stress factors typically found in Feed Processing environments. Moreover, the role of dominant resident flora strains in the biofilm development of Salmonella was determined. Results Surface microflora characterization from two Feed productions plants, by means of 16 S rDNA sequencing, revealed a wide diversity of bacteria. Survival, disinfection and biofilm formation experiments were conducted on selected dominant resident flora strains and Salmonella . Results showed higher survival properties by resident flora isolates for desiccation, and disinfection compared to Salmonella isolates. Dual-species biofilms favored Salmonella growth compared to Salmonella in mono-species biofilms, with biovolume increases of 2.8-fold and 3.2-fold in the presence of Staphylococcus and Pseudomonas , respectively. Conclusions These results offer an overview of the microflora composition found in Feed industry Processing environments, their survival under relevant stresses and their potential effect on biofilm formation in the presence of Salmonella . Eliminating the establishment of resident flora isolates in Feed industry surfaces is therefore of interest for impeding conditions for Salmonella colonization and growth on Feed industry surfaces. In-depth investigations are still needed to determine whether resident flora has a definite role in the persistence of Salmonella in Feed Processing environments.
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micro ecosystems from Feed industry surfaces a survival and biofilm study of salmonella versus host resident flora strains
BMC Veterinary Research, 2010Co-Authors: Olivier Habimana, Trond Moretro, Lene K Vestby, Live L Nesse, Solveig Langsrud, Even HeirAbstract:Background The presence of Salmonella enterica serovars in Feed ingredients, products and Processing facilities is a well recognized problem worldwide. In Norwegian Feed factories, strict control measures are implemented to avoid establishment and spreading of Salmonella throughout the Processing chain. There is limited knowledge on the presence and survival of the resident microflora in Feed production plants. Information on interactions between Salmonella and other bacteria in Feed production plants and how they affect survival and biofilm formation of Salmonella is also limited. The aim of this study was to identify resident microbiota found in Feed production environments, and to compare the survival of resident flora strains and Salmonella to stress factors typically found in Feed Processing environments. Moreover, the role of dominant resident flora strains in the biofilm development of Salmonella was determined.
Solveig Langsrud - One of the best experts on this subject based on the ideXlab platform.
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The persistence of Salmonella following desiccation under Feed Processing environmental conditions: a subject of relevance.
Letters in Applied Microbiology, 2014Co-Authors: Olivier Habimana, Trond Moretro, Lene K Vestby, Live L Nesse, Even Heir, Kristin Berg, Solveig LangsrudAbstract:UNLABELLED Although Salmonella persistence has been predominantly linked to biofilm formation, the physiological state of Salmonella should also be considered as a possible pathway for persistence and survival in the Feed industry. Hence, the purpose of this study was to assess the extent of viability of Salmonella cells through long-term desiccation periods under conditions typically found in Feed Processing environments, and whether these same cells could resuscitate and cause salmonellosis in vivo. We showed that upon desiccation, Salmonella Agona, a representative Feed industry isolate and Salmonella Typhimurium ATCC 14028, a laboratory strain, were induced into a nonculturable state at 35 and 85% relative humidity conditions, at defined temperatures of 30 and 12°C, respectively. Although the reduction in culturable cells was more than 6 log10 , metabolic activity was found in more than 1% of the population. Desiccation-induced nonculturable Salm. Typhimurium could not be revived and were nonvirulent in a mouse model following infection through oral gavage. These results suggest that the specific conditions for reviving nonculturable Salmonella after long periods of desiccation are yet to be fully identified. The need for mapping key factors involved in the persistence of Salmonella would help better detect it and improve Feed safety measures. SIGNIFICANCE AND IMPACT OF THE STUDY While Salmonella has been shown to persist for years in Feed Processing environments, it is still unknown how temperature and humidity affect the persistence of Salmonella cells over time in terms of their metabolic states and cultivability. Here, we show that long-term exposure to Feed Processing environmental conditions induces Salmonella into a nonculturable state even though about 1% of the population remains metabolically active. This has significant implications when monitoring Salmonella from the environment which could yield false-negative results using conventional pre-enrichment detection methods.
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Micro ecosystems from Feed industry surfaces: a survival and biofilm study of Salmonellaversus host resident flora strains
BMC Veterinary Research, 2010Co-Authors: Olivier Habimana, Trond Moretro, Lene K Vestby, Live L Nesse, Solveig Langsrud, Even HeirAbstract:Background The presence of Salmonella enterica serovars in Feed ingredients, products and Processing facilities is a well recognized problem worldwide. In Norwegian Feed factories, strict control measures are implemented to avoid establishment and spreading of Salmonella throughout the Processing chain. There is limited knowledge on the presence and survival of the resident microflora in Feed production plants. Information on interactions between Salmonella and other bacteria in Feed production plants and how they affect survival and biofilm formation of Salmonella is also limited. The aim of this study was to identify resident microbiota found in Feed production environments, and to compare the survival of resident flora strains and Salmonella to stress factors typically found in Feed Processing environments. Moreover, the role of dominant resident flora strains in the biofilm development of Salmonella was determined. Results Surface microflora characterization from two Feed productions plants, by means of 16 S rDNA sequencing, revealed a wide diversity of bacteria. Survival, disinfection and biofilm formation experiments were conducted on selected dominant resident flora strains and Salmonella . Results showed higher survival properties by resident flora isolates for desiccation, and disinfection compared to Salmonella isolates. Dual-species biofilms favored Salmonella growth compared to Salmonella in mono-species biofilms, with biovolume increases of 2.8-fold and 3.2-fold in the presence of Staphylococcus and Pseudomonas , respectively. Conclusions These results offer an overview of the microflora composition found in Feed industry Processing environments, their survival under relevant stresses and their potential effect on biofilm formation in the presence of Salmonella . Eliminating the establishment of resident flora isolates in Feed industry surfaces is therefore of interest for impeding conditions for Salmonella colonization and growth on Feed industry surfaces. In-depth investigations are still needed to determine whether resident flora has a definite role in the persistence of Salmonella in Feed Processing environments.
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micro ecosystems from Feed industry surfaces a survival and biofilm study of salmonella versus host resident flora strains
BMC Veterinary Research, 2010Co-Authors: Olivier Habimana, Trond Moretro, Lene K Vestby, Live L Nesse, Solveig Langsrud, Even HeirAbstract:Background The presence of Salmonella enterica serovars in Feed ingredients, products and Processing facilities is a well recognized problem worldwide. In Norwegian Feed factories, strict control measures are implemented to avoid establishment and spreading of Salmonella throughout the Processing chain. There is limited knowledge on the presence and survival of the resident microflora in Feed production plants. Information on interactions between Salmonella and other bacteria in Feed production plants and how they affect survival and biofilm formation of Salmonella is also limited. The aim of this study was to identify resident microbiota found in Feed production environments, and to compare the survival of resident flora strains and Salmonella to stress factors typically found in Feed Processing environments. Moreover, the role of dominant resident flora strains in the biofilm development of Salmonella was determined.
Trond Moretro - One of the best experts on this subject based on the ideXlab platform.
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The persistence of Salmonella following desiccation under Feed Processing environmental conditions: a subject of relevance.
Letters in Applied Microbiology, 2014Co-Authors: Olivier Habimana, Trond Moretro, Lene K Vestby, Live L Nesse, Even Heir, Kristin Berg, Solveig LangsrudAbstract:UNLABELLED Although Salmonella persistence has been predominantly linked to biofilm formation, the physiological state of Salmonella should also be considered as a possible pathway for persistence and survival in the Feed industry. Hence, the purpose of this study was to assess the extent of viability of Salmonella cells through long-term desiccation periods under conditions typically found in Feed Processing environments, and whether these same cells could resuscitate and cause salmonellosis in vivo. We showed that upon desiccation, Salmonella Agona, a representative Feed industry isolate and Salmonella Typhimurium ATCC 14028, a laboratory strain, were induced into a nonculturable state at 35 and 85% relative humidity conditions, at defined temperatures of 30 and 12°C, respectively. Although the reduction in culturable cells was more than 6 log10 , metabolic activity was found in more than 1% of the population. Desiccation-induced nonculturable Salm. Typhimurium could not be revived and were nonvirulent in a mouse model following infection through oral gavage. These results suggest that the specific conditions for reviving nonculturable Salmonella after long periods of desiccation are yet to be fully identified. The need for mapping key factors involved in the persistence of Salmonella would help better detect it and improve Feed safety measures. SIGNIFICANCE AND IMPACT OF THE STUDY While Salmonella has been shown to persist for years in Feed Processing environments, it is still unknown how temperature and humidity affect the persistence of Salmonella cells over time in terms of their metabolic states and cultivability. Here, we show that long-term exposure to Feed Processing environmental conditions induces Salmonella into a nonculturable state even though about 1% of the population remains metabolically active. This has significant implications when monitoring Salmonella from the environment which could yield false-negative results using conventional pre-enrichment detection methods.
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Micro ecosystems from Feed industry surfaces: a survival and biofilm study of Salmonellaversus host resident flora strains
BMC Veterinary Research, 2010Co-Authors: Olivier Habimana, Trond Moretro, Lene K Vestby, Live L Nesse, Solveig Langsrud, Even HeirAbstract:Background The presence of Salmonella enterica serovars in Feed ingredients, products and Processing facilities is a well recognized problem worldwide. In Norwegian Feed factories, strict control measures are implemented to avoid establishment and spreading of Salmonella throughout the Processing chain. There is limited knowledge on the presence and survival of the resident microflora in Feed production plants. Information on interactions between Salmonella and other bacteria in Feed production plants and how they affect survival and biofilm formation of Salmonella is also limited. The aim of this study was to identify resident microbiota found in Feed production environments, and to compare the survival of resident flora strains and Salmonella to stress factors typically found in Feed Processing environments. Moreover, the role of dominant resident flora strains in the biofilm development of Salmonella was determined. Results Surface microflora characterization from two Feed productions plants, by means of 16 S rDNA sequencing, revealed a wide diversity of bacteria. Survival, disinfection and biofilm formation experiments were conducted on selected dominant resident flora strains and Salmonella . Results showed higher survival properties by resident flora isolates for desiccation, and disinfection compared to Salmonella isolates. Dual-species biofilms favored Salmonella growth compared to Salmonella in mono-species biofilms, with biovolume increases of 2.8-fold and 3.2-fold in the presence of Staphylococcus and Pseudomonas , respectively. Conclusions These results offer an overview of the microflora composition found in Feed industry Processing environments, their survival under relevant stresses and their potential effect on biofilm formation in the presence of Salmonella . Eliminating the establishment of resident flora isolates in Feed industry surfaces is therefore of interest for impeding conditions for Salmonella colonization and growth on Feed industry surfaces. In-depth investigations are still needed to determine whether resident flora has a definite role in the persistence of Salmonella in Feed Processing environments.
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micro ecosystems from Feed industry surfaces a survival and biofilm study of salmonella versus host resident flora strains
BMC Veterinary Research, 2010Co-Authors: Olivier Habimana, Trond Moretro, Lene K Vestby, Live L Nesse, Solveig Langsrud, Even HeirAbstract:Background The presence of Salmonella enterica serovars in Feed ingredients, products and Processing facilities is a well recognized problem worldwide. In Norwegian Feed factories, strict control measures are implemented to avoid establishment and spreading of Salmonella throughout the Processing chain. There is limited knowledge on the presence and survival of the resident microflora in Feed production plants. Information on interactions between Salmonella and other bacteria in Feed production plants and how they affect survival and biofilm formation of Salmonella is also limited. The aim of this study was to identify resident microbiota found in Feed production environments, and to compare the survival of resident flora strains and Salmonella to stress factors typically found in Feed Processing environments. Moreover, the role of dominant resident flora strains in the biofilm development of Salmonella was determined.
Lene K Vestby - One of the best experts on this subject based on the ideXlab platform.
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The persistence of Salmonella following desiccation under Feed Processing environmental conditions: a subject of relevance.
Letters in Applied Microbiology, 2014Co-Authors: Olivier Habimana, Trond Moretro, Lene K Vestby, Live L Nesse, Even Heir, Kristin Berg, Solveig LangsrudAbstract:UNLABELLED Although Salmonella persistence has been predominantly linked to biofilm formation, the physiological state of Salmonella should also be considered as a possible pathway for persistence and survival in the Feed industry. Hence, the purpose of this study was to assess the extent of viability of Salmonella cells through long-term desiccation periods under conditions typically found in Feed Processing environments, and whether these same cells could resuscitate and cause salmonellosis in vivo. We showed that upon desiccation, Salmonella Agona, a representative Feed industry isolate and Salmonella Typhimurium ATCC 14028, a laboratory strain, were induced into a nonculturable state at 35 and 85% relative humidity conditions, at defined temperatures of 30 and 12°C, respectively. Although the reduction in culturable cells was more than 6 log10 , metabolic activity was found in more than 1% of the population. Desiccation-induced nonculturable Salm. Typhimurium could not be revived and were nonvirulent in a mouse model following infection through oral gavage. These results suggest that the specific conditions for reviving nonculturable Salmonella after long periods of desiccation are yet to be fully identified. The need for mapping key factors involved in the persistence of Salmonella would help better detect it and improve Feed safety measures. SIGNIFICANCE AND IMPACT OF THE STUDY While Salmonella has been shown to persist for years in Feed Processing environments, it is still unknown how temperature and humidity affect the persistence of Salmonella cells over time in terms of their metabolic states and cultivability. Here, we show that long-term exposure to Feed Processing environmental conditions induces Salmonella into a nonculturable state even though about 1% of the population remains metabolically active. This has significant implications when monitoring Salmonella from the environment which could yield false-negative results using conventional pre-enrichment detection methods.
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Micro ecosystems from Feed industry surfaces: a survival and biofilm study of Salmonellaversus host resident flora strains
BMC Veterinary Research, 2010Co-Authors: Olivier Habimana, Trond Moretro, Lene K Vestby, Live L Nesse, Solveig Langsrud, Even HeirAbstract:Background The presence of Salmonella enterica serovars in Feed ingredients, products and Processing facilities is a well recognized problem worldwide. In Norwegian Feed factories, strict control measures are implemented to avoid establishment and spreading of Salmonella throughout the Processing chain. There is limited knowledge on the presence and survival of the resident microflora in Feed production plants. Information on interactions between Salmonella and other bacteria in Feed production plants and how they affect survival and biofilm formation of Salmonella is also limited. The aim of this study was to identify resident microbiota found in Feed production environments, and to compare the survival of resident flora strains and Salmonella to stress factors typically found in Feed Processing environments. Moreover, the role of dominant resident flora strains in the biofilm development of Salmonella was determined. Results Surface microflora characterization from two Feed productions plants, by means of 16 S rDNA sequencing, revealed a wide diversity of bacteria. Survival, disinfection and biofilm formation experiments were conducted on selected dominant resident flora strains and Salmonella . Results showed higher survival properties by resident flora isolates for desiccation, and disinfection compared to Salmonella isolates. Dual-species biofilms favored Salmonella growth compared to Salmonella in mono-species biofilms, with biovolume increases of 2.8-fold and 3.2-fold in the presence of Staphylococcus and Pseudomonas , respectively. Conclusions These results offer an overview of the microflora composition found in Feed industry Processing environments, their survival under relevant stresses and their potential effect on biofilm formation in the presence of Salmonella . Eliminating the establishment of resident flora isolates in Feed industry surfaces is therefore of interest for impeding conditions for Salmonella colonization and growth on Feed industry surfaces. In-depth investigations are still needed to determine whether resident flora has a definite role in the persistence of Salmonella in Feed Processing environments.
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micro ecosystems from Feed industry surfaces a survival and biofilm study of salmonella versus host resident flora strains
BMC Veterinary Research, 2010Co-Authors: Olivier Habimana, Trond Moretro, Lene K Vestby, Live L Nesse, Solveig Langsrud, Even HeirAbstract:Background The presence of Salmonella enterica serovars in Feed ingredients, products and Processing facilities is a well recognized problem worldwide. In Norwegian Feed factories, strict control measures are implemented to avoid establishment and spreading of Salmonella throughout the Processing chain. There is limited knowledge on the presence and survival of the resident microflora in Feed production plants. Information on interactions between Salmonella and other bacteria in Feed production plants and how they affect survival and biofilm formation of Salmonella is also limited. The aim of this study was to identify resident microbiota found in Feed production environments, and to compare the survival of resident flora strains and Salmonella to stress factors typically found in Feed Processing environments. Moreover, the role of dominant resident flora strains in the biofilm development of Salmonella was determined.