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Thierry Baron - One of the best experts on this subject based on the ideXlab platform.
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T: Possible case of maternal transmission of Feline Spongiform Encephalopathy in a captive cheetah. PLoS One 2009
2016Co-Authors: Anna Bencsik, Sabine O.s. Debeer, Thierry Petit, Thierry BaronAbstract:Feline Spongiform Encephalopathy (FSE) is considered to be related to bovine Spongiform Encephalopathy (BSE) and has been reported in domestic cats as well as in captive wild cats including cheetahs, first in the United Kingdom (UK) and then in other European countries. In France, several cases were described in cheetahs either imported from UK or born in France. Here we report details of two other FSE cases in captive cheetah including a 2nd case of FSE in a cheetah born in France, most likely due to maternal transmission. Complete prion protein immunohistochemical study on both brains and peripheral organs showed the close likeness between the two cases. In addition, transmission studies to the TgOvPrP4 mouse line were also performed, for comparison with the transmission of cattle BSE. The TgOvPrP4 mouse brains infected with cattle BSE and cheetah FSE revealed similar vacuolar lesion profiles, PrPd brain mapping with occurrence of typical florid plaques. Collectively, these data indicate that they harbor the same strain of agent as the cattle BSE agent. This new observation may have some impact on our knowledge of vertical transmission of BSE agent-linked TSEs such as in housecat FSE, or vCJD
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Is the presence of abnormal prion protein in the renal glomeruli of Feline species presenting with FSE authentic
BMC veterinary research, 2010Co-Authors: Stéphane Lezmi, Thierry Baron, Anna BencsikAbstract:In a recent paper written by Hilbe et al (BMC vet res, 2009), the nature and specificity of the prion protein deposition in the kidney of Feline species affected with Feline Spongiform Encephalopathy (FSE) were clearly considered doubtful. This article was brought to our attention because we published several years ago an immunodetection of abnormal prion protein in the kidney of a cheetah affected with FSE. At this time we were convinced of its specificity but without having all the possibilities to demonstrate it. As previously published by another group, the presence of abnormal prion protein in some renal glomeruli in domestic cats affected with FSE is indeed generally considered as doubtful mainly because of low intensity detected in this organ and because control kidneys from safe animals present also a weak prion immunolabelling. Here we come back on these studies and thought it would be helpful to relay our last data to the readers of BMC Vet res for future reference on this subject.
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Possible Case of Maternal Transmission of Feline Spongiform Encephalopathy in a Captive Cheetah
PloS one, 2009Co-Authors: Anna Bencsik, Sabine O.s. Debeer, Thierry Petit, Thierry BaronAbstract:Feline Spongiform Encephalopathy (FSE) is considered to be related to bovine Spongiform Encephalopathy (BSE) and has been reported in domestic cats as well as in captive wild cats including cheetahs, first in the United Kingdom (UK) and then in other European countries. In France, several cases were described in cheetahs either imported from UK or born in France. Here we report details of two other FSE cases in captive cheetah including a 2nd case of FSE in a cheetah born in France, most likely due to maternal transmission. Complete prion protein immunohistochemical study on both brains and peripheral organs showed the close likeness between the two cases. In addition, transmission studies to the TgOvPrP4 mouse line were also performed, for comparison with the transmission of cattle BSE. The TgOvPrP4 mouse brains infected with cattle BSE and cheetah FSE revealed similar vacuolar lesion profiles, PrPd brain mapping with occurrence of typical florid plaques. Collectively, these data indicate that they harbor the same strain of agent as the cattle BSE agent. This new observation may have some impact on our knowledge of vertical transmission of BSE agent-linked TSEs such as in housecat FSE, or vCJD.
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PET-blot Analysis Contributes to BSE Strain Recognition in C57Bl/6 Mice
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 2006Co-Authors: Stéphane Lezmi, Anna Bencsik, Thierry BaronAbstract:Identification of the strain of agent responsible for bovine Spongiform Encephalopathy (BSE) can be made histologically through the analysis of both distribution and intensity of brain vacuolar lesions after BSE transmission to mouse. Another useful way to distinguish the BSE agent from other prion strains is the study of the distribution of the abnormal prion protein (PrPres). For that purpose, paraffin-embedded tissue blot (PET-blot) method was applied on brains from C57Bl/6 mice infected with cattle BSE, experimental sheep BSE, or Feline Spongiform Encephalopathy (FSE) from a cheetah. PrPres distribution was comparable, whichever of the three BSE agent sources was considered and was distinct from the PrPres distribution in C57Bl/6 mice inoculated with a French scrapie isolate or with a mouse-adapted scrapie strain (C506M3). These data confirm a common origin of infectious agent responsible for the British and French cattle BSE. They also indicate that PET-blot method appears as a precise complementary tool in prion strain studies because it offers easy and quick assessment of the PrPres mapping. Advantages and limits of the PET-blot method are discussed and compared with other established and validated methods of strain typing.
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First case of Feline Spongiform Encephalopathy in a captive cheetah born in France: PrPsc analysis in various tissues revealed unexpected targeting of kidney and adrenal gland
Histochemistry and cell biology, 2003Co-Authors: Stéphane Lezmi, Anna Bencsik, Thierry Petit, Eoin Monks, Thierry BaronAbstract:Feline Spongiform Encephalopathy (FSE), affecting domestic and captive Feline species, is a prion disease considered to be related to bovine Spongiform Encephalopathy. Here we report an immunohistological analysis of the first FSE-affected cheetah born in France. The duration of clinical signs, of which ataxia was the main one, was about 8 weeks. The distribution of abnormal prion protein (PrPsc) was studied by immunohistochemistry within 27 different tissues. Different antibodies were used to visualise abnormal PrP deposits in situ. PrPsc accumulation was detected in the central nervous system (cerebral cortex, cerebellum, brain stem, spinal cord, retina), in peripheral nerves and in lymphoid organs. PrPsc deposits were not observed within the enteric nervous system nor in several other organs, such as pancreas, ovary, liver and muscle. More interestingly, unusual PrPsc deposits were observed within the zona fasciculata/reticularis of the adrenal gland and within some glomeruli of the kidney raising the question of possible PrPsc excretion. The sympathetic innervation of these two organs was visualised and compared to the distribution of PrPsc deposits. Our results suggest the possibility that the infectious agent is spread by both haematogenous and nervous pathways.
Stéphane Lezmi - One of the best experts on this subject based on the ideXlab platform.
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Is the presence of abnormal prion protein in the renal glomeruli of Feline species presenting with FSE authentic
BMC veterinary research, 2010Co-Authors: Stéphane Lezmi, Thierry Baron, Anna BencsikAbstract:In a recent paper written by Hilbe et al (BMC vet res, 2009), the nature and specificity of the prion protein deposition in the kidney of Feline species affected with Feline Spongiform Encephalopathy (FSE) were clearly considered doubtful. This article was brought to our attention because we published several years ago an immunodetection of abnormal prion protein in the kidney of a cheetah affected with FSE. At this time we were convinced of its specificity but without having all the possibilities to demonstrate it. As previously published by another group, the presence of abnormal prion protein in some renal glomeruli in domestic cats affected with FSE is indeed generally considered as doubtful mainly because of low intensity detected in this organ and because control kidneys from safe animals present also a weak prion immunolabelling. Here we come back on these studies and thought it would be helpful to relay our last data to the readers of BMC Vet res for future reference on this subject.
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Is the presence of abnormal prion protein in the renal glomeruli of Feline species presenting with FSE authentic?
BMC Veterinary Research, 2010Co-Authors: Stéphane Lezmi, Thierry Gm Baron, Anna A BencsikAbstract:In a recent paper written by Hilbe et al (BMC vet res, 2009), the nature and specificity of the prion protein deposition in the kidney of Feline species affected with Feline Spongiform Encephalopathy (FSE) were clearly considered doubtful. This article was brought to our attention because we published several years ago an immunodetection of abnormal prion protein in the kidney of a cheetah affected with FSE. At this time we were convinced of its specificity but without having all the possibilities to demonstrate it. As previously published by another group, the presence of abnormal prion protein in some renal glomeruli in domestic cats affected with FSE is indeed generally considered as doubtful mainly because of low intensity detected in this organ and because control kidneys from safe animals present also a weak prion immunolabelling. Here we come back on these studies and thought it would be helpful to relay our last data to the readers of BMC Vet res for future reference on this subject. Here we come back on our material as it is possible to study and demonstrate the specificity of prion immunodetection using the PET-Blot method (Paraffin Embedded Tissue - Blot). It is admitted that this method allows detecting the Proteinase K (PK) resistant form of the abnormal prion protein (PrPres) without any confusion with unspecific immunoreaction. We re-analysed the kidney tissue versus adrenal gland and brain samples from the same cheetah affected with TSE using this PET-Blot method. The PET-Blot analysis revealed specific PrPres detection within the brain, adrenal gland and some glomeruli of the kidney, with a complete identicalness compared to our previous detection using immunohistochemistry. In conclusion, these new data enable us to confirm with assurance the presence of specific abnormal prion protein in the adrenal gland and in the kidney of the cheetah affected with FSE. It also emphasizes the usefulness for the re-examination of any available tissue blocks with the PET-Blot method as a sensitive complementary tool in case of doubtful PrP IHC results.
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PET-blot Analysis Contributes to BSE Strain Recognition in C57Bl/6 Mice
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 2006Co-Authors: Stéphane Lezmi, Anna Bencsik, Thierry BaronAbstract:Identification of the strain of agent responsible for bovine Spongiform Encephalopathy (BSE) can be made histologically through the analysis of both distribution and intensity of brain vacuolar lesions after BSE transmission to mouse. Another useful way to distinguish the BSE agent from other prion strains is the study of the distribution of the abnormal prion protein (PrPres). For that purpose, paraffin-embedded tissue blot (PET-blot) method was applied on brains from C57Bl/6 mice infected with cattle BSE, experimental sheep BSE, or Feline Spongiform Encephalopathy (FSE) from a cheetah. PrPres distribution was comparable, whichever of the three BSE agent sources was considered and was distinct from the PrPres distribution in C57Bl/6 mice inoculated with a French scrapie isolate or with a mouse-adapted scrapie strain (C506M3). These data confirm a common origin of infectious agent responsible for the British and French cattle BSE. They also indicate that PET-blot method appears as a precise complementary tool in prion strain studies because it offers easy and quick assessment of the PrPres mapping. Advantages and limits of the PET-blot method are discussed and compared with other established and validated methods of strain typing.
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First case of Feline Spongiform Encephalopathy in a captive cheetah born in France: PrPsc analysis in various tissues revealed unexpected targeting of kidney and adrenal gland
Histochemistry and cell biology, 2003Co-Authors: Stéphane Lezmi, Anna Bencsik, Thierry Petit, Eoin Monks, Thierry BaronAbstract:Feline Spongiform Encephalopathy (FSE), affecting domestic and captive Feline species, is a prion disease considered to be related to bovine Spongiform Encephalopathy. Here we report an immunohistological analysis of the first FSE-affected cheetah born in France. The duration of clinical signs, of which ataxia was the main one, was about 8 weeks. The distribution of abnormal prion protein (PrPsc) was studied by immunohistochemistry within 27 different tissues. Different antibodies were used to visualise abnormal PrP deposits in situ. PrPsc accumulation was detected in the central nervous system (cerebral cortex, cerebellum, brain stem, spinal cord, retina), in peripheral nerves and in lymphoid organs. PrPsc deposits were not observed within the enteric nervous system nor in several other organs, such as pancreas, ovary, liver and muscle. More interestingly, unusual PrPsc deposits were observed within the zona fasciculata/reticularis of the adrenal gland and within some glomeruli of the kidney raising the question of possible PrPsc excretion. The sympathetic innervation of these two organs was visualised and compared to the distribution of PrPsc deposits. Our results suggest the possibility that the infectious agent is spread by both haematogenous and nervous pathways.
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First case of Feline Spongiform Encephalopathy in a captive cheetah born in France: PrP^sc analysis in various tissues revealed unexpected targeting of kidney and adrenal gland
Histochemistry and Cell Biology, 2003Co-Authors: Stéphane Lezmi, Anna Bencsik, Thierry Petit, Eoin Monks, Thierry BaronAbstract:Feline Spongiform Encephalopathy (FSE), affecting domestic and captive Feline species, is a prion disease considered to be related to bovine Spongiform Encephalopathy. Here we report an immunohistological analysis of the first FSE-affected cheetah born in France. The duration of clinical signs, of which ataxia was the main one, was about 8 weeks. The distribution of abnormal prion protein (PrP^sc) was studied by immunohistochemistry within 27 different tissues. Different antibodies were used to visualise abnormal PrP deposits in situ. PrP^sc accumulation was detected in the central nervous system (cerebral cortex, cerebellum, brain stem, spinal cord, retina), in peripheral nerves and in lymphoid organs. PrP^sc deposits were not observed within the enteric nervous system nor in several other organs, such as pancreas, ovary, liver and muscle. More interestingly, unusual PrP^sc deposits were observed within the zona fasciculata/reticularis of the adrenal gland and within some glomeruli of the kidney raising the question of possible PrP^sc excretion. The sympathetic innervation of these two organs was visualised and compared to the distribution of PrP^sc deposits. Our results suggest the possibility that the infectious agent is spread by both haematogenous and nervous pathways.
Anna Bencsik - One of the best experts on this subject based on the ideXlab platform.
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T: Possible case of maternal transmission of Feline Spongiform Encephalopathy in a captive cheetah. PLoS One 2009
2016Co-Authors: Anna Bencsik, Sabine O.s. Debeer, Thierry Petit, Thierry BaronAbstract:Feline Spongiform Encephalopathy (FSE) is considered to be related to bovine Spongiform Encephalopathy (BSE) and has been reported in domestic cats as well as in captive wild cats including cheetahs, first in the United Kingdom (UK) and then in other European countries. In France, several cases were described in cheetahs either imported from UK or born in France. Here we report details of two other FSE cases in captive cheetah including a 2nd case of FSE in a cheetah born in France, most likely due to maternal transmission. Complete prion protein immunohistochemical study on both brains and peripheral organs showed the close likeness between the two cases. In addition, transmission studies to the TgOvPrP4 mouse line were also performed, for comparison with the transmission of cattle BSE. The TgOvPrP4 mouse brains infected with cattle BSE and cheetah FSE revealed similar vacuolar lesion profiles, PrPd brain mapping with occurrence of typical florid plaques. Collectively, these data indicate that they harbor the same strain of agent as the cattle BSE agent. This new observation may have some impact on our knowledge of vertical transmission of BSE agent-linked TSEs such as in housecat FSE, or vCJD
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Is the presence of abnormal prion protein in the renal glomeruli of Feline species presenting with FSE authentic
BMC veterinary research, 2010Co-Authors: Stéphane Lezmi, Thierry Baron, Anna BencsikAbstract:In a recent paper written by Hilbe et al (BMC vet res, 2009), the nature and specificity of the prion protein deposition in the kidney of Feline species affected with Feline Spongiform Encephalopathy (FSE) were clearly considered doubtful. This article was brought to our attention because we published several years ago an immunodetection of abnormal prion protein in the kidney of a cheetah affected with FSE. At this time we were convinced of its specificity but without having all the possibilities to demonstrate it. As previously published by another group, the presence of abnormal prion protein in some renal glomeruli in domestic cats affected with FSE is indeed generally considered as doubtful mainly because of low intensity detected in this organ and because control kidneys from safe animals present also a weak prion immunolabelling. Here we come back on these studies and thought it would be helpful to relay our last data to the readers of BMC Vet res for future reference on this subject.
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Possible Case of Maternal Transmission of Feline Spongiform Encephalopathy in a Captive Cheetah
PloS one, 2009Co-Authors: Anna Bencsik, Sabine O.s. Debeer, Thierry Petit, Thierry BaronAbstract:Feline Spongiform Encephalopathy (FSE) is considered to be related to bovine Spongiform Encephalopathy (BSE) and has been reported in domestic cats as well as in captive wild cats including cheetahs, first in the United Kingdom (UK) and then in other European countries. In France, several cases were described in cheetahs either imported from UK or born in France. Here we report details of two other FSE cases in captive cheetah including a 2nd case of FSE in a cheetah born in France, most likely due to maternal transmission. Complete prion protein immunohistochemical study on both brains and peripheral organs showed the close likeness between the two cases. In addition, transmission studies to the TgOvPrP4 mouse line were also performed, for comparison with the transmission of cattle BSE. The TgOvPrP4 mouse brains infected with cattle BSE and cheetah FSE revealed similar vacuolar lesion profiles, PrPd brain mapping with occurrence of typical florid plaques. Collectively, these data indicate that they harbor the same strain of agent as the cattle BSE agent. This new observation may have some impact on our knowledge of vertical transmission of BSE agent-linked TSEs such as in housecat FSE, or vCJD.
-
PET-blot Analysis Contributes to BSE Strain Recognition in C57Bl/6 Mice
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 2006Co-Authors: Stéphane Lezmi, Anna Bencsik, Thierry BaronAbstract:Identification of the strain of agent responsible for bovine Spongiform Encephalopathy (BSE) can be made histologically through the analysis of both distribution and intensity of brain vacuolar lesions after BSE transmission to mouse. Another useful way to distinguish the BSE agent from other prion strains is the study of the distribution of the abnormal prion protein (PrPres). For that purpose, paraffin-embedded tissue blot (PET-blot) method was applied on brains from C57Bl/6 mice infected with cattle BSE, experimental sheep BSE, or Feline Spongiform Encephalopathy (FSE) from a cheetah. PrPres distribution was comparable, whichever of the three BSE agent sources was considered and was distinct from the PrPres distribution in C57Bl/6 mice inoculated with a French scrapie isolate or with a mouse-adapted scrapie strain (C506M3). These data confirm a common origin of infectious agent responsible for the British and French cattle BSE. They also indicate that PET-blot method appears as a precise complementary tool in prion strain studies because it offers easy and quick assessment of the PrPres mapping. Advantages and limits of the PET-blot method are discussed and compared with other established and validated methods of strain typing.
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First case of Feline Spongiform Encephalopathy in a captive cheetah born in France: PrPsc analysis in various tissues revealed unexpected targeting of kidney and adrenal gland
Histochemistry and cell biology, 2003Co-Authors: Stéphane Lezmi, Anna Bencsik, Thierry Petit, Eoin Monks, Thierry BaronAbstract:Feline Spongiform Encephalopathy (FSE), affecting domestic and captive Feline species, is a prion disease considered to be related to bovine Spongiform Encephalopathy. Here we report an immunohistological analysis of the first FSE-affected cheetah born in France. The duration of clinical signs, of which ataxia was the main one, was about 8 weeks. The distribution of abnormal prion protein (PrPsc) was studied by immunohistochemistry within 27 different tissues. Different antibodies were used to visualise abnormal PrP deposits in situ. PrPsc accumulation was detected in the central nervous system (cerebral cortex, cerebellum, brain stem, spinal cord, retina), in peripheral nerves and in lymphoid organs. PrPsc deposits were not observed within the enteric nervous system nor in several other organs, such as pancreas, ovary, liver and muscle. More interestingly, unusual PrPsc deposits were observed within the zona fasciculata/reticularis of the adrenal gland and within some glomeruli of the kidney raising the question of possible PrPsc excretion. The sympathetic innervation of these two organs was visualised and compared to the distribution of PrPsc deposits. Our results suggest the possibility that the infectious agent is spread by both haematogenous and nervous pathways.
Thierry Petit - One of the best experts on this subject based on the ideXlab platform.
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T: Possible case of maternal transmission of Feline Spongiform Encephalopathy in a captive cheetah. PLoS One 2009
2016Co-Authors: Anna Bencsik, Sabine O.s. Debeer, Thierry Petit, Thierry BaronAbstract:Feline Spongiform Encephalopathy (FSE) is considered to be related to bovine Spongiform Encephalopathy (BSE) and has been reported in domestic cats as well as in captive wild cats including cheetahs, first in the United Kingdom (UK) and then in other European countries. In France, several cases were described in cheetahs either imported from UK or born in France. Here we report details of two other FSE cases in captive cheetah including a 2nd case of FSE in a cheetah born in France, most likely due to maternal transmission. Complete prion protein immunohistochemical study on both brains and peripheral organs showed the close likeness between the two cases. In addition, transmission studies to the TgOvPrP4 mouse line were also performed, for comparison with the transmission of cattle BSE. The TgOvPrP4 mouse brains infected with cattle BSE and cheetah FSE revealed similar vacuolar lesion profiles, PrPd brain mapping with occurrence of typical florid plaques. Collectively, these data indicate that they harbor the same strain of agent as the cattle BSE agent. This new observation may have some impact on our knowledge of vertical transmission of BSE agent-linked TSEs such as in housecat FSE, or vCJD
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Possible Case of Maternal Transmission of Feline Spongiform Encephalopathy in a Captive Cheetah
PloS one, 2009Co-Authors: Anna Bencsik, Sabine O.s. Debeer, Thierry Petit, Thierry BaronAbstract:Feline Spongiform Encephalopathy (FSE) is considered to be related to bovine Spongiform Encephalopathy (BSE) and has been reported in domestic cats as well as in captive wild cats including cheetahs, first in the United Kingdom (UK) and then in other European countries. In France, several cases were described in cheetahs either imported from UK or born in France. Here we report details of two other FSE cases in captive cheetah including a 2nd case of FSE in a cheetah born in France, most likely due to maternal transmission. Complete prion protein immunohistochemical study on both brains and peripheral organs showed the close likeness between the two cases. In addition, transmission studies to the TgOvPrP4 mouse line were also performed, for comparison with the transmission of cattle BSE. The TgOvPrP4 mouse brains infected with cattle BSE and cheetah FSE revealed similar vacuolar lesion profiles, PrPd brain mapping with occurrence of typical florid plaques. Collectively, these data indicate that they harbor the same strain of agent as the cattle BSE agent. This new observation may have some impact on our knowledge of vertical transmission of BSE agent-linked TSEs such as in housecat FSE, or vCJD.
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First case of Feline Spongiform Encephalopathy in a captive cheetah born in France: PrPsc analysis in various tissues revealed unexpected targeting of kidney and adrenal gland
Histochemistry and cell biology, 2003Co-Authors: Stéphane Lezmi, Anna Bencsik, Thierry Petit, Eoin Monks, Thierry BaronAbstract:Feline Spongiform Encephalopathy (FSE), affecting domestic and captive Feline species, is a prion disease considered to be related to bovine Spongiform Encephalopathy. Here we report an immunohistological analysis of the first FSE-affected cheetah born in France. The duration of clinical signs, of which ataxia was the main one, was about 8 weeks. The distribution of abnormal prion protein (PrPsc) was studied by immunohistochemistry within 27 different tissues. Different antibodies were used to visualise abnormal PrP deposits in situ. PrPsc accumulation was detected in the central nervous system (cerebral cortex, cerebellum, brain stem, spinal cord, retina), in peripheral nerves and in lymphoid organs. PrPsc deposits were not observed within the enteric nervous system nor in several other organs, such as pancreas, ovary, liver and muscle. More interestingly, unusual PrPsc deposits were observed within the zona fasciculata/reticularis of the adrenal gland and within some glomeruli of the kidney raising the question of possible PrPsc excretion. The sympathetic innervation of these two organs was visualised and compared to the distribution of PrPsc deposits. Our results suggest the possibility that the infectious agent is spread by both haematogenous and nervous pathways.
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First case of Feline Spongiform Encephalopathy in a captive cheetah born in France: PrP^sc analysis in various tissues revealed unexpected targeting of kidney and adrenal gland
Histochemistry and Cell Biology, 2003Co-Authors: Stéphane Lezmi, Anna Bencsik, Thierry Petit, Eoin Monks, Thierry BaronAbstract:Feline Spongiform Encephalopathy (FSE), affecting domestic and captive Feline species, is a prion disease considered to be related to bovine Spongiform Encephalopathy. Here we report an immunohistological analysis of the first FSE-affected cheetah born in France. The duration of clinical signs, of which ataxia was the main one, was about 8 weeks. The distribution of abnormal prion protein (PrP^sc) was studied by immunohistochemistry within 27 different tissues. Different antibodies were used to visualise abnormal PrP deposits in situ. PrP^sc accumulation was detected in the central nervous system (cerebral cortex, cerebellum, brain stem, spinal cord, retina), in peripheral nerves and in lymphoid organs. PrP^sc deposits were not observed within the enteric nervous system nor in several other organs, such as pancreas, ovary, liver and muscle. More interestingly, unusual PrP^sc deposits were observed within the zona fasciculata/reticularis of the adrenal gland and within some glomeruli of the kidney raising the question of possible PrP^sc excretion. The sympathetic innervation of these two organs was visualised and compared to the distribution of PrP^sc deposits. Our results suggest the possibility that the infectious agent is spread by both haematogenous and nervous pathways.
Eoin Monks - One of the best experts on this subject based on the ideXlab platform.
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First case of Feline Spongiform Encephalopathy in a captive cheetah born in France: PrPsc analysis in various tissues revealed unexpected targeting of kidney and adrenal gland
Histochemistry and cell biology, 2003Co-Authors: Stéphane Lezmi, Anna Bencsik, Thierry Petit, Eoin Monks, Thierry BaronAbstract:Feline Spongiform Encephalopathy (FSE), affecting domestic and captive Feline species, is a prion disease considered to be related to bovine Spongiform Encephalopathy. Here we report an immunohistological analysis of the first FSE-affected cheetah born in France. The duration of clinical signs, of which ataxia was the main one, was about 8 weeks. The distribution of abnormal prion protein (PrPsc) was studied by immunohistochemistry within 27 different tissues. Different antibodies were used to visualise abnormal PrP deposits in situ. PrPsc accumulation was detected in the central nervous system (cerebral cortex, cerebellum, brain stem, spinal cord, retina), in peripheral nerves and in lymphoid organs. PrPsc deposits were not observed within the enteric nervous system nor in several other organs, such as pancreas, ovary, liver and muscle. More interestingly, unusual PrPsc deposits were observed within the zona fasciculata/reticularis of the adrenal gland and within some glomeruli of the kidney raising the question of possible PrPsc excretion. The sympathetic innervation of these two organs was visualised and compared to the distribution of PrPsc deposits. Our results suggest the possibility that the infectious agent is spread by both haematogenous and nervous pathways.
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First case of Feline Spongiform Encephalopathy in a captive cheetah born in France: PrP^sc analysis in various tissues revealed unexpected targeting of kidney and adrenal gland
Histochemistry and Cell Biology, 2003Co-Authors: Stéphane Lezmi, Anna Bencsik, Thierry Petit, Eoin Monks, Thierry BaronAbstract:Feline Spongiform Encephalopathy (FSE), affecting domestic and captive Feline species, is a prion disease considered to be related to bovine Spongiform Encephalopathy. Here we report an immunohistological analysis of the first FSE-affected cheetah born in France. The duration of clinical signs, of which ataxia was the main one, was about 8 weeks. The distribution of abnormal prion protein (PrP^sc) was studied by immunohistochemistry within 27 different tissues. Different antibodies were used to visualise abnormal PrP deposits in situ. PrP^sc accumulation was detected in the central nervous system (cerebral cortex, cerebellum, brain stem, spinal cord, retina), in peripheral nerves and in lymphoid organs. PrP^sc deposits were not observed within the enteric nervous system nor in several other organs, such as pancreas, ovary, liver and muscle. More interestingly, unusual PrP^sc deposits were observed within the zona fasciculata/reticularis of the adrenal gland and within some glomeruli of the kidney raising the question of possible PrP^sc excretion. The sympathetic innervation of these two organs was visualised and compared to the distribution of PrP^sc deposits. Our results suggest the possibility that the infectious agent is spread by both haematogenous and nervous pathways.
