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Gary Williamson - One of the best experts on this subject based on the ideXlab platform.

  • Release of Ferulic Acid from wheat bran by a Ferulic Acid esterase (FAE-III) from Aspergillus niger
    Applied microbiology and biotechnology, 1995
    Co-Authors: Craig B. Faulds, Gary Williamson
    Abstract:

    Ferulic Acid was efficiently released from a wheat bran preparation by a Ferulic Acid esterase from Aspergillus niger (FAE-III) when incubated together with a Trichoderma viride xylanase (a maximum of 95% total Ferulic Acid released after 5 h incubation). FAE-III by itself could release Ferulic Acid but at a level almost 24-fold lower than that obtained in the presence of the xylanase (2 U). Release of Ferulic Acid was proportional to the FAE-III concentration between 0.1 U and 1.3 U, but the presence of low levels of xylanase (0.1 U) increased the amount of Ferulic Acid released 6-fold. Total sugar release was not influenced by the action of FAE-III on the wheat bran, but the rate of release of the apparent end-products of xylanase action (xylose and xylobiose) was elevated by the presence of the esterase. The results show that FAE-III and the xylanase act together to break down feruloylated plant cell-wall polysaccharides to give a high yield of Ferulic Acid.

  • Release of Ferulic Acid from plant polysaccharides by Ferulic Acid esterase from Streptomyces olivochromogenes
    Carbohydrate Polymers, 1993
    Co-Authors: Craig B. Faulds, Gary Williamson
    Abstract:

    Abstract Streptomyces olivochromogenes Ferulic Acid esterase released free Ferulic Acid from 0-[5-0-(trans-feruloyl)-α- l -arabinofuranosyl]-(1→3)-0-β-xylopyranosyl-(1→4)- d -xylopyranose (FAXX) prepared from wheat bran. The Km and Vmax of the reaction were 0·24 m m and 0·134 U mg−1, respectively. The enzyme released 0·25% of the covalently bound Ferulic Acid from sugar beet pectin both in the presence and in the absence of pectinase.

K. Madhavan Nampoothiri - One of the best experts on this subject based on the ideXlab platform.

  • Biorefining of wheat bran for the purification of Ferulic Acid
    Biocatalysis and Agricultural Biotechnology, 2018
    Co-Authors: Nishant Gopalan, K. Madhavan Nampoothiri
    Abstract:

    Abstract Feruloyl esterase, a versatile enzyme has been used for extracting an abundant phenolic Acid, viz, Ferulic Acid (FA) from destarched wheat bran. The study probes into the dependency of various parameters including enzyme loading, reaction time, pH and temperature on the extracted amount of Ferulic Acid. Statistical optimization of the process improved the extraction yield by 2.5 times and obtained a maximum of 34.6% of total alkali releasable Ferulic Acid was extracted and when the process is scaled up in a packed column reactor, it was able to extract 32.5% within12 h. The study has also focused on finding a good adsorbent for the purification of Ferulic Acid, which ended up in the use of AMBERLITE XAD4 resin owing to its capacity of binding 49.9 mg FA /g resin and obtained an impressive 99.5% recovery using methanol as an eluent.

J.-f. Thibault - One of the best experts on this subject based on the ideXlab platform.

  • studies on enzymic release of Ferulic Acid from sugar beet pulp
    Lwt - Food Science and Technology, 1994
    Co-Authors: Valérie Micard, Catherine M.g.c. Renard, J.-f. Thibault
    Abstract:

    Abstract Eighteen commercial enzymic preparations have been tested for their ability to release Ferulic Acid from primary cell-walls of sugar-beet pulp. Three release patterns were found: (a) no or little, esterified Ferulic Acid; (b) only esterified Ferulic Acid; or (c) a mixture of free and esterified Ferulic Acid. No enzymic complex released only free Ferulic Acid. Release kinetics were studied on two complexes, 'Hemicellulase REG II' (Gist-Brocades), which released the highest amount of esterified Ferulic Acid, and 'Pektolase LM' (Grindsted), which released one of the highest amounts of free Ferulic Acid with the lowest proportion of esterified Ferulic Acid. 'Pektolase LM' digested sugar-beet pulp rapidly, first releasing esterified Ferulic Acid, which was later deesterified. 'Hemicellulase REG II' showed an initial lag phase followed by fast liberation (between 32 and 48 h of incubation) of esterified Ferulic Acid. Sugar-beet pulp hydrolysates from 'Pektolase LM' and 'Hemicellulase REG II' were fractionated on DEAE Sephacel. The results showed that esterified Ferulic Acid was present either linked to fragments of pectic polysaccharides poor in arabinose (∼40% of esterified Ferulic Acid injected) or to arabinose oligomers (∼60%).

Craig B. Faulds - One of the best experts on this subject based on the ideXlab platform.

  • Release of Ferulic Acid from wheat bran by a Ferulic Acid esterase (FAE-III) from Aspergillus niger
    Applied microbiology and biotechnology, 1995
    Co-Authors: Craig B. Faulds, Gary Williamson
    Abstract:

    Ferulic Acid was efficiently released from a wheat bran preparation by a Ferulic Acid esterase from Aspergillus niger (FAE-III) when incubated together with a Trichoderma viride xylanase (a maximum of 95% total Ferulic Acid released after 5 h incubation). FAE-III by itself could release Ferulic Acid but at a level almost 24-fold lower than that obtained in the presence of the xylanase (2 U). Release of Ferulic Acid was proportional to the FAE-III concentration between 0.1 U and 1.3 U, but the presence of low levels of xylanase (0.1 U) increased the amount of Ferulic Acid released 6-fold. Total sugar release was not influenced by the action of FAE-III on the wheat bran, but the rate of release of the apparent end-products of xylanase action (xylose and xylobiose) was elevated by the presence of the esterase. The results show that FAE-III and the xylanase act together to break down feruloylated plant cell-wall polysaccharides to give a high yield of Ferulic Acid.

  • Release of Ferulic Acid from plant polysaccharides by Ferulic Acid esterase from Streptomyces olivochromogenes
    Carbohydrate Polymers, 1993
    Co-Authors: Craig B. Faulds, Gary Williamson
    Abstract:

    Abstract Streptomyces olivochromogenes Ferulic Acid esterase released free Ferulic Acid from 0-[5-0-(trans-feruloyl)-α- l -arabinofuranosyl]-(1→3)-0-β-xylopyranosyl-(1→4)- d -xylopyranose (FAXX) prepared from wheat bran. The Km and Vmax of the reaction were 0·24 m m and 0·134 U mg−1, respectively. The enzyme released 0·25% of the covalently bound Ferulic Acid from sugar beet pectin both in the presence and in the absence of pectinase.

Ping Xu - One of the best experts on this subject based on the ideXlab platform.

  • enhanced vanillin production from Ferulic Acid using adsorbent resin
    Applied Microbiology and Biotechnology, 2007
    Co-Authors: Lifu Song, Zhaobin Zhang, Zixin Deng, Ping Xu
    Abstract:

    High vanillin productivity was achieved in the batch biotransformation of Ferulic Acid by Streptomyces sp. strain V-1. Due to the toxicity of vanillin and the product inhibition, fed-batch biotransformation with high concentration of Ferulic Acid was unsuccessful. To solve this problem and improve the vanillin yield, a biotransformation strategy using adsorbent resin was investigated. Several macroporous adsorbent resins were chosen to adsorb vanillin in situ during the bioconversion. Resin DM11 was found to be the best, which adsorbed the most vanillin and the least Ferulic Acid. When 8% resin DM11 (wet w/v) was added to the biotransformation system, 45 g l−1 Ferulic Acid could be added continually and 19.2 g l−1 vanillin was obtained within 55 h, which was the highest vanillin yield by bioconversion until now. This yield was remarkable for exceeding the crystallization concentration of vanillin and therefore had far-reaching consequence in its downstream processing.