The Experts below are selected from a list of 240 Experts worldwide ranked by ideXlab platform
Ezequiel Goldschmidt - One of the best experts on this subject based on the ideXlab platform.
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the effect of vancomycin powder on human dural Fibroblast Culture and its implications for dural repair during spine surgery
Journal of Neurosurgery, 2016Co-Authors: Monica Loresi, Ezequiel Goldschmidt, Jorge Rasmussen, Joseph D Chabot, Gurpreet S Gandhoke, Emilia Luzzi, Lina Merlotti, Romina Proni, Kojo D HamiltonAbstract:OBJECTIVE Surgical site infections (SSIs) are a major source of morbidity after spinal surgery. Several recent studies have described the finding that applying vancomycin powder to the surgical bed may reduce the incidence of SSI. However, applying vancomycin in high concentrations has been shown in vitro to inhibit osteoblast proliferation and to induce cell death. Vancomycin may have a deleterious effect on dural healing after repair of an intentional or unintentional durotomy. This study was therefore undertaken to assess the effect of different concentrations of vancomycin on a human dura mater cell Culture. METHODS Human dura intended for disposal after decompressive craniectomy was harvested. Explant primary Cultures and subCultures were subsequently performed. Cells were characterized through common staining and immunohistochemistry. A growth curve was performed to assess the effect of different concentrations of vancomycin (40, 400, and 4000 μg/ml) on cell count. The effect of vancomycin on cellul...
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the effect of vancomycin powder on human dural Fibroblast Culture and its implications for dural repair during spine surgery
Journal of Neurosurgery, 2016Co-Authors: Monica Loresi, Ezequiel Goldschmidt, Jorge Rasmussen, Joseph D Chabot, Gurpreet S Gandhoke, Emilia Luzzi, Lina Merlotti, Romina Proni, Kojo D HamiltonAbstract:OBJECTIVE Surgical site infections (SSIs) are a major source of morbidity after spinal surgery. Several recent studies have described the finding that applying vancomycin powder to the surgical bed may reduce the incidence of SSI. However, applying vancomycin in high concentrations has been shown in vitro to inhibit osteoblast proliferation and to induce cell death. Vancomycin may have a deleterious effect on dural healing after repair of an intentional or unintentional durotomy. This study was therefore undertaken to assess the effect of different concentrations of vancomycin on a human dura mater cell Culture. METHODS Human dura intended for disposal after decompressive craniectomy was harvested. Explant primary Cultures and subCultures were subsequently performed. Cells were characterized through common staining and immunohistochemistry. A growth curve was performed to assess the effect of different concentrations of vancomycin (40, 400, and 4000 μg/ml) on cell count. The effect of vancomycin on cellular shape, intercellular arrangement, and viability was also evaluated. RESULTS All dural tissue samples successfully developed into fusiform cells, demonstrating pseudopod projections and spindle formation. The cells demonstrated vimentin positivity and also had typical features of Fibroblasts. When applied to the Cultures, the highest dose of vancomycin induced generalized cell death within 24 hours. The mean (± SD) cell counts for control, 40, 400, and 4000 μg/ml were 38.72 ± 15.93, 36.28 ± 22.87, 19.48 ± 6.53, and 4.07 ± 9.66, respectively (p < 0.0001, ANOVA). Compared with controls, vancomycin-exposed cells histologically demonstrated a smaller cytoplasm and decreased pseudopodia formation resulting in the inhibition of normal spindle intercellular arrangement. CONCLUSIONS When vancomycin powder is applied locally, dural cells are exposed to a concentration several times greater than when delivered systemically. In this in vitro model, vancomycin induced dural cell death, inhibited growth, and altered cellular morphology in a concentration-dependent fashion. Defining a safe vancomycin concentration that is both bactericidal and also does not inhibit normal dural healing is necessary.
Kojo D Hamilton - One of the best experts on this subject based on the ideXlab platform.
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the effect of vancomycin powder on human dural Fibroblast Culture and its implications for dural repair during spine surgery
Journal of Neurosurgery, 2016Co-Authors: Monica Loresi, Ezequiel Goldschmidt, Jorge Rasmussen, Joseph D Chabot, Gurpreet S Gandhoke, Emilia Luzzi, Lina Merlotti, Romina Proni, Kojo D HamiltonAbstract:OBJECTIVE Surgical site infections (SSIs) are a major source of morbidity after spinal surgery. Several recent studies have described the finding that applying vancomycin powder to the surgical bed may reduce the incidence of SSI. However, applying vancomycin in high concentrations has been shown in vitro to inhibit osteoblast proliferation and to induce cell death. Vancomycin may have a deleterious effect on dural healing after repair of an intentional or unintentional durotomy. This study was therefore undertaken to assess the effect of different concentrations of vancomycin on a human dura mater cell Culture. METHODS Human dura intended for disposal after decompressive craniectomy was harvested. Explant primary Cultures and subCultures were subsequently performed. Cells were characterized through common staining and immunohistochemistry. A growth curve was performed to assess the effect of different concentrations of vancomycin (40, 400, and 4000 μg/ml) on cell count. The effect of vancomycin on cellul...
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the effect of vancomycin powder on human dural Fibroblast Culture and its implications for dural repair during spine surgery
Journal of Neurosurgery, 2016Co-Authors: Monica Loresi, Ezequiel Goldschmidt, Jorge Rasmussen, Joseph D Chabot, Gurpreet S Gandhoke, Emilia Luzzi, Lina Merlotti, Romina Proni, Kojo D HamiltonAbstract:OBJECTIVE Surgical site infections (SSIs) are a major source of morbidity after spinal surgery. Several recent studies have described the finding that applying vancomycin powder to the surgical bed may reduce the incidence of SSI. However, applying vancomycin in high concentrations has been shown in vitro to inhibit osteoblast proliferation and to induce cell death. Vancomycin may have a deleterious effect on dural healing after repair of an intentional or unintentional durotomy. This study was therefore undertaken to assess the effect of different concentrations of vancomycin on a human dura mater cell Culture. METHODS Human dura intended for disposal after decompressive craniectomy was harvested. Explant primary Cultures and subCultures were subsequently performed. Cells were characterized through common staining and immunohistochemistry. A growth curve was performed to assess the effect of different concentrations of vancomycin (40, 400, and 4000 μg/ml) on cell count. The effect of vancomycin on cellular shape, intercellular arrangement, and viability was also evaluated. RESULTS All dural tissue samples successfully developed into fusiform cells, demonstrating pseudopod projections and spindle formation. The cells demonstrated vimentin positivity and also had typical features of Fibroblasts. When applied to the Cultures, the highest dose of vancomycin induced generalized cell death within 24 hours. The mean (± SD) cell counts for control, 40, 400, and 4000 μg/ml were 38.72 ± 15.93, 36.28 ± 22.87, 19.48 ± 6.53, and 4.07 ± 9.66, respectively (p < 0.0001, ANOVA). Compared with controls, vancomycin-exposed cells histologically demonstrated a smaller cytoplasm and decreased pseudopodia formation resulting in the inhibition of normal spindle intercellular arrangement. CONCLUSIONS When vancomycin powder is applied locally, dural cells are exposed to a concentration several times greater than when delivered systemically. In this in vitro model, vancomycin induced dural cell death, inhibited growth, and altered cellular morphology in a concentration-dependent fashion. Defining a safe vancomycin concentration that is both bactericidal and also does not inhibit normal dural healing is necessary.
Emmett Pinney - One of the best experts on this subject based on the ideXlab platform.
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Three-Dimensional Fibroblast Culture Implant for the Treatment of Diabetic Foot Ulcers: Metabolic Activity and Therapeutic Range
Tissue Engineering, 1998Co-Authors: Jonathan Mansbridge, Ruth Patch, Kent Symons, Emmett PinneyAbstract:Dermagraft is three-dimensional, allogeneic, human neonatal dermal Fibroblast Culture grown on a degradable scaffold and cryopreserved. Clinical trials for treatment of diabetic foot ulcers showed optimal healing within a therapeutic range of metabolic activity, determined by 3[4,5-dimethylthiazol-2-yl]2,5-diphenyltetrazolium bromide (MTT) reduction. Actions of Dermagraft in wound repair include colonization by cells and provision of growth factors and cytokines, both activities dependent on living cells. Cells in the cryopreserved Culture showed 60% viability by dye exclusion and, when isolated, were able to proliferate in monolayer Culture. Protein synthesis by Dermagraft was inhibited 70–98% by cryopreservation, but, if within the therapeutic range, recovered to 45–85% of the prefreeze value over 48 h. Subtherapeutic Dermagraft showed variable, low recovery. Expression of mRNA for vascular endothelial growth factor (VEGF), platelet–derived growth factor A chain, and insulin–like growth factor–1 was red...
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three dimensional Fibroblast Culture implant for the treatment of diabetic foot ulcers metabolic activity and therapeutic range
Tissue Engineering, 1998Co-Authors: Jonathan Mansbridge, Ruth Patch, Kent Symons, Kang Liu, Emmett PinneyAbstract:Dermagraft is three-dimensional, allogeneic, human neonatal dermal Fibroblast Culture grown on a degradable scaffold and cryopreserved. Clinical trials for treatment of diabetic foot ulcers showed optimal healing within a therapeutic range of metabolic activity, determined by 3[4,5-dimethylthiazol-2-yl]2,5-diphenyltetrazolium bromide (MTT) reduction. Actions of Dermagraft in wound repair include colonization by cells and provision of growth factors and cytokines, both activities dependent on living cells. Cells in the cryopreserved Culture showed 60% viability by dye exclusion and, when isolated, were able to proliferate in monolayer Culture. Protein synthesis by Dermagraft was inhibited 70-98% by cryopreservation, but, if within the therapeutic range, recovered to 45-85% of the prefreeze value over 48 h. Subtherapeutic Dermagraft showed variable, low recovery. Expression of mRNA for vascular endothelial growth factor (VEGF), platelet-derived growth factor A chain, and insulin-like growth factor-1 was reduced >83% in subtherapeutic compared with therapeutic Dermagraft. Granulocyte colony-stimulating factor and VEGF protein secretion, determined by enzyme-linked immunosorbent assay (ELISA), and angiogenic activity also depended on therapeutic range. VEGF secretion dropped sharply with MTT reductase in subtherapeutic tissue. The data demonstrate the critical dependence of the therapeutic properties of this living dermal implant on recovery of protein synthesis, growth factor expression, and angiogenesis, determined by metabolic activity.
Jonathan Mansbridge - One of the best experts on this subject based on the ideXlab platform.
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Three-Dimensional Fibroblast Culture Implant for the Treatment of Diabetic Foot Ulcers: Metabolic Activity and Therapeutic Range
Tissue Engineering, 1998Co-Authors: Jonathan Mansbridge, Ruth Patch, Kent Symons, Emmett PinneyAbstract:Dermagraft is three-dimensional, allogeneic, human neonatal dermal Fibroblast Culture grown on a degradable scaffold and cryopreserved. Clinical trials for treatment of diabetic foot ulcers showed optimal healing within a therapeutic range of metabolic activity, determined by 3[4,5-dimethylthiazol-2-yl]2,5-diphenyltetrazolium bromide (MTT) reduction. Actions of Dermagraft in wound repair include colonization by cells and provision of growth factors and cytokines, both activities dependent on living cells. Cells in the cryopreserved Culture showed 60% viability by dye exclusion and, when isolated, were able to proliferate in monolayer Culture. Protein synthesis by Dermagraft was inhibited 70–98% by cryopreservation, but, if within the therapeutic range, recovered to 45–85% of the prefreeze value over 48 h. Subtherapeutic Dermagraft showed variable, low recovery. Expression of mRNA for vascular endothelial growth factor (VEGF), platelet–derived growth factor A chain, and insulin–like growth factor–1 was red...
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three dimensional Fibroblast Culture implant for the treatment of diabetic foot ulcers metabolic activity and therapeutic range
Tissue Engineering, 1998Co-Authors: Jonathan Mansbridge, Ruth Patch, Kent Symons, Kang Liu, Emmett PinneyAbstract:Dermagraft is three-dimensional, allogeneic, human neonatal dermal Fibroblast Culture grown on a degradable scaffold and cryopreserved. Clinical trials for treatment of diabetic foot ulcers showed optimal healing within a therapeutic range of metabolic activity, determined by 3[4,5-dimethylthiazol-2-yl]2,5-diphenyltetrazolium bromide (MTT) reduction. Actions of Dermagraft in wound repair include colonization by cells and provision of growth factors and cytokines, both activities dependent on living cells. Cells in the cryopreserved Culture showed 60% viability by dye exclusion and, when isolated, were able to proliferate in monolayer Culture. Protein synthesis by Dermagraft was inhibited 70-98% by cryopreservation, but, if within the therapeutic range, recovered to 45-85% of the prefreeze value over 48 h. Subtherapeutic Dermagraft showed variable, low recovery. Expression of mRNA for vascular endothelial growth factor (VEGF), platelet-derived growth factor A chain, and insulin-like growth factor-1 was reduced >83% in subtherapeutic compared with therapeutic Dermagraft. Granulocyte colony-stimulating factor and VEGF protein secretion, determined by enzyme-linked immunosorbent assay (ELISA), and angiogenic activity also depended on therapeutic range. VEGF secretion dropped sharply with MTT reductase in subtherapeutic tissue. The data demonstrate the critical dependence of the therapeutic properties of this living dermal implant on recovery of protein synthesis, growth factor expression, and angiogenesis, determined by metabolic activity.
Anatoliy Goltsev - One of the best experts on this subject based on the ideXlab platform.
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Application of Cryopreserved Fibroblast Culture with Au Nanoparticles to Treat Burns
Nanoscale Research Letters, 2016Co-Authors: Nataliia Volkova, Mariia Yukhta, Olena Pavlovich, Anatoliy GoltsevAbstract:The aim was to investigate a possibility of using the cryopreserved human Culture of Fibroblasts (CrHFC) with gold nanoparticles (AuNPs) to treat experimental burns in rats. The third-degree burns were modeled in white male rats. All the animals with burns were divided into three experimental groups: control group with no wound treatment; group 1 was composed of animals with CrHFC application; and group 2 consisted of those with CrHFC and AuNPs (6 μg/ml) application to a burn surface the next day after the injury. The CrHFC was applied to the methylcellulose gel in a dose of 5 × 10^4 of viable cells per 1 cm^2 of the burn. The animals were removed from the experiment on day 21 after the treatment. The CrHFC use alone and with AuNPs to the surface of burns stimulated the wound healing compared with the control. The effect of using CrHFC was less pronounced compared to the CrHFC application with AuNPs. It was reflected in a slower recovery of burns and moderate lymphocytic infiltration of granulation tissue. Immunofluorescent analysis emphasized that the use of CrHFC with AuNPs accelerated the skin synthetic processes and was helpful in recovering type I and III collagen content on day 21 after therapy. The results were likely related primarily to the unique structure and antimicrobial properties of AuNPs. Our experimental study of the effect of CrHFC with AuNPs application on regenerative processes in burns gives some pre-conditions to the following advanced bio- and nanotechnology developments.
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application of cryopreserved Fibroblast Culture with au nanoparticles to treat burns
Nanoscale Research Letters, 2016Co-Authors: N A Volkova, Mariia Yukhta, Olena Pavlovich, Anatoliy GoltsevAbstract:The aim was to investigate a possibility of using the cryopreserved human Culture of Fibroblasts (CrHFC) with gold nanoparticles (AuNPs) to treat experimental burns in rats.