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Dewa Judhistira Guntara - One of the best experts on this subject based on the ideXlab platform.
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KONSENTRASI HAMBAT DAN BUNUH MINIMUM FORMULA HIDROGEL EKSTRAK DAUN TIN (Ficus Carica) TERHADAP PERTUMBUHAN STAPHYLOCOCCUS AUREUS
Fakultas Kedokteran Gigi Universitas Mahasaraswati Denpasar, 2020Co-Authors: Dewa Judhistira GuntaraAbstract:Staphylococcus aureus merupakan patogen yang dapat menyebabkan berbagai infeksi sekunder seperti infeksi pada kulit dan jaringan lunak di rongga mulut, dilaporkan bahwa S. aureus memiliki peran dalam kegagalan implan gigi, menginfeksi perawatan saluran akar dan juga dapat berkolonisasi pada mukosa pengguna gigi tiruan. Penanganan akibat infeksi S. aureus biasanya menggunakan antibiotik, tetapi penggunaan antibiotik secara berlebihan akan menyebabkan perubahan ekologi dan menimbulkan resistensi di rongga mulut. Daun Tin (Ficus Carica) yang diekstrak dibuat dalam sedian hidrogel dan dapat dijadikan obat karena memiliki kandungan senyawa aktif seperti alkaloid, flavonoid, kumarin, saponin, polifenol dan tanin yang dapat menghambat dan membunuh bakteri dan berfungsi sebagai antibakteri. Tujuan penelitian ini untuk mengetahui Konsentrasi Hambat Minimum (KHM) dan Konsentrasi Bunuh Minimum (KBM) formula hidrogel ekstrak daun Tin (Ficus Carica) terhadap pertumbuhan Staphylococcus aureus. Daun Tin (Ficus Carica) diekstraksi dengan metode maserasi, selanjutnya dilakukan uji konsentrasi hambat dan bunuh minimum dengan metode dilusi. Berdasarkan hasil uji Kruskal-Wallis diperoleh nilai P = 0,63 (P>0,05) yang menunjukan tidak terdapat perbedaan yang bermakna antara kelompok perlakuan terhadap pertumbuhan S. aureus. Hasil perlakuan menunjukan nilai tertinggi sampai terendah yaitu kontrol negatif, 5%, 25%, 50%, 100% dan kontrol positif. Kesimpulan penelitian ini didapatkan konsentrasi formula hidrogel ekstrak daun Tin (Ficus Carica) dalam menghambat S. aureus adalah pada konsentrasi 5% dan Konsentrasi Bunuh Minimum formula hidrogel ekstrak daun Tin (Ficus Carica) pada ekstrak murni 100%.Banda Ace
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KONSENTRASI HAMBAT DAN BUNUH MINIMUM FORMULA HIDROGEL EKSTRAK DAUN TIN (Ficus Carica) TERHADAP PERTUMBUHAN STAPHYLOCOCCUS AUREUS
Fakultas Kedokteran Gigi Universitas Mahasaraswati Denpasar, 2020Co-Authors: Dewa Judhistira GuntaraAbstract:Staphylococcus aureus merupakan patogen yang dapat menyebabkan berbagai infeksi sekunder seperti infeksi pada kulit dan jaringan lunak di rongga mulut, dilaporkan bahwa S. aureus memiliki peran dalam kegagalan implan gigi, menginfeksi perawatan saluran akar dan juga dapat berkolonisasi pada mukosa pengguna gigi tiruan. Penanganan akibat infeksi S. aureus biasanya menggunakan antibiotik, tetapi penggunaan antibiotik secara berlebihan akan menyebabkan perubahan ekologi dan menimbulkan resistensi di rongga mulut. Daun Tin (Ficus Carica) yang diekstrak dibuat dalam sedian hidrogel dan dapat dijadikan obat karena memiliki kandungan senyawa aktif seperti alkaloid, flavonoid, kumarin, saponin, polifenol dan tanin yang dapat menghambat dan membunuh bakteri dan berfungsi sebagai antibakteri. Tujuan penelitian ini untuk mengetahui Konsentrasi Hambat Minimum (KHM) dan Konsentrasi Bunuh Minimum (KBM) formula hidrogel ekstrak daun Tin (Ficus Carica) terhadap pertumbuhan Staphylococcus aureus. Daun Tin (Ficus Carica) diekstraksi dengan metode maserasi, selanjutnya dilakukan uji konsentrasi hambat dan bunuh minimum dengan metode dilusi. Berdasarkan hasil uji Kruskal-Wallis diperoleh nilai P = 0,63 (P>0,05) yang menunjukan tidak terdapat perbedaan yang bermakna antara kelompok perlakuan terhadap pertumbuhan S. aureus. Hasil perlakuan menunjukan nilai tertinggi sampai terendah yaitu kontrol negatif, 5%, 25%, 50%, 100% dan kontrol positif. Kesimpulan penelitian ini didapatkan konsentrasi formula hidrogel ekstrak daun Tin (Ficus Carica) dalam menghambat S. aureus adalah pada konsentrasi 5%, dan pada konsentrasi 100% tidak menunjukan hasil uji yang sama seperti kontrol positif.Kata Kunci : Staphylococcus aureus, Hidrogel, Daun Tin (Ficus Carica), Konsentrasi Hambat Minimum (KHM) dan Konsentrasi Bunuh Minimum (KBM)Banda Ace
V B Kudachikar - One of the best experts on this subject based on the ideXlab platform.
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calcium chloride extends the keeping quality of fig fruit Ficus Carica l during storage and shelf life
Postharvest Biology and Technology, 2013Co-Authors: P K Irfan, V Vanjakshi, M Keshava N Prakash, R Ravi, V B KudachikarAbstract:Abstract The effects of postharvest application of fruit hardening chemical agents on fig (Ficus Carica L. cv. Poona) fruit were compared with untreated figs during storage. The impact of calcium chloride (4%) was notable in terms of retention of fruit color, texture and increased accumulation of ascorbic acid, compared to untreated control figs. Pretreatment with calcium chloride (4%) was found to be most effective in checking the growth of both mesophilic aerobic bacteria and yeast and molds at low temperature (1 ± 0.5 °C; 95–98% RH) storage and it further delayed ripening and senescence of figs and was beneficial in prolonging the postharvest life twofold. Treated figs without microbial spoilage could be used for short term storage, transportation, distribution and marketing for long distance domestic markets in India.
Cesar A Jara - One of the best experts on this subject based on the ideXlab platform.
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efecto del extracto etanolico de Ficus Carica moraceae sobre la formacion de la larva 2 de ascaris suum y trichuris ovis en condiciones de laboratorio
REVISTA REBIOL, 2016Co-Authors: Karen Siccha Aguilar, Victor Teran, Cesar A JaraAbstract:Las infecciones parasitarias por helmintos causan disminucion en la produccion pecuaria y, ante la aparicion de resistencia antihelmintica al uso de quimicos, surge la necesidad de indagar respecto de la capacidad antiparasitaria de extractos de plantas hasta ahora no utilizados. En la presente investigacion se evaluo el efecto del extracto etanolico de las hojas de Ficus Carica “higo” (Moraceae), sobre la formacion de la larva 2 (L2) de Ascaris suum y Trichuris ovis ; para ello, se obtuvieron huevos fertiles no embrionados del utero de hembras gravidas de ambas especies, las que fueron colocadas en Placas de Petri, aproximadamente mil huevos por placa: en tres de ellas (E1, E2 y E3) con el extracto, a las concentraciones de 4000, 2000 y 1000 ppm y una cuarta (C) con solucion salina fisiologica (el experimento se hizo por duplicado). Las palcas fueron mantenidas a temperatura ambiente (25-26°C) y cada 48 horas se hicieron observaciones microscopicas para verificar la formacion de las L2. A los 20 dias, en C se obtuvo 2.4% de huevos sin L2 de A. suum y 2.3% de T. ovis, y en E1 se observo el mayor porcentaje de huevos sin L2 de ambas especies: 38.8% y 32.7%, respectivamente (p<0.05). Se concluye que el extracto etanolico de F. Carica inhibe la formacion de la larva 2 de A. suum y T. ovis, a los 20 dias post tratamiento, en condiciones de laboratorio. Palabras clave: extracto etanolico, Ficus Carica (Moraceae), Ascaris suum, Trichuris ovis
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efecto del extracto etanolico de Ficus Carica moraceae sobre la formacion de la larva 2 de ascaris suum y trichuris ovis en condiciones de laboratorio effect of the ethanol extract of Ficus Carica moraceae on the formation of ascaris suum and trichur
2015Co-Authors: Karen Siccha Aguilar, Victor Teran, Cesar A JaraAbstract:Parasitic helminth infections cause decrease in livestock production and, before the emergence of resistance to anthelmintic use of chemicals, the need to inquire about the antiparasitic capacity of plant extracts so far unused. In this research the effect of ethanol extract of the leaves of Ficus Carica (Moraceae) on the formation of the larva 2 (L2) of Ascaris suum and Trichuris ovis was assessed; for this purpose, fertile eggs, not embryonated, were obtained the uterus of pregnant females of both species, which were placed in Petri dishes, about thousand eggs per plate: three of which (E1, E2 and E3) with the extract, at the concentrations of 4000, 2000 and 1000 ppm and a fourth (C) with physiological saline (the experiment was done in duplicate). The Plates were maintained at room temperature (25-26 ° C) and every 48 hours microscopic observations were made to verify the formation of L2. At 20 days, 2.4% C without L2 eggs of A. suum and 2.3% of T. ovis was obtained, and E1 the highest percentage of eggs without L2 of both species were observed, 38.8% and 32.7%, respectively (p <0.05). It is concluded that the Ficus Carica-ethanol extract inhibits the formation of A. suum and T. ovis larva 2 at 20 days post treatment, under laboratory conditions.
P K Irfan - One of the best experts on this subject based on the ideXlab platform.
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calcium chloride extends the keeping quality of fig fruit Ficus Carica l during storage and shelf life
Postharvest Biology and Technology, 2013Co-Authors: P K Irfan, V Vanjakshi, M Keshava N Prakash, R Ravi, V B KudachikarAbstract:Abstract The effects of postharvest application of fruit hardening chemical agents on fig (Ficus Carica L. cv. Poona) fruit were compared with untreated figs during storage. The impact of calcium chloride (4%) was notable in terms of retention of fruit color, texture and increased accumulation of ascorbic acid, compared to untreated control figs. Pretreatment with calcium chloride (4%) was found to be most effective in checking the growth of both mesophilic aerobic bacteria and yeast and molds at low temperature (1 ± 0.5 °C; 95–98% RH) storage and it further delayed ripening and senescence of figs and was beneficial in prolonging the postharvest life twofold. Treated figs without microbial spoilage could be used for short term storage, transportation, distribution and marketing for long distance domestic markets in India.
Karen Siccha Aguilar - One of the best experts on this subject based on the ideXlab platform.
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efecto del extracto etanolico de Ficus Carica moraceae sobre la formacion de la larva 2 de ascaris suum y trichuris ovis en condiciones de laboratorio
REVISTA REBIOL, 2016Co-Authors: Karen Siccha Aguilar, Victor Teran, Cesar A JaraAbstract:Las infecciones parasitarias por helmintos causan disminucion en la produccion pecuaria y, ante la aparicion de resistencia antihelmintica al uso de quimicos, surge la necesidad de indagar respecto de la capacidad antiparasitaria de extractos de plantas hasta ahora no utilizados. En la presente investigacion se evaluo el efecto del extracto etanolico de las hojas de Ficus Carica “higo” (Moraceae), sobre la formacion de la larva 2 (L2) de Ascaris suum y Trichuris ovis ; para ello, se obtuvieron huevos fertiles no embrionados del utero de hembras gravidas de ambas especies, las que fueron colocadas en Placas de Petri, aproximadamente mil huevos por placa: en tres de ellas (E1, E2 y E3) con el extracto, a las concentraciones de 4000, 2000 y 1000 ppm y una cuarta (C) con solucion salina fisiologica (el experimento se hizo por duplicado). Las palcas fueron mantenidas a temperatura ambiente (25-26°C) y cada 48 horas se hicieron observaciones microscopicas para verificar la formacion de las L2. A los 20 dias, en C se obtuvo 2.4% de huevos sin L2 de A. suum y 2.3% de T. ovis, y en E1 se observo el mayor porcentaje de huevos sin L2 de ambas especies: 38.8% y 32.7%, respectivamente (p<0.05). Se concluye que el extracto etanolico de F. Carica inhibe la formacion de la larva 2 de A. suum y T. ovis, a los 20 dias post tratamiento, en condiciones de laboratorio. Palabras clave: extracto etanolico, Ficus Carica (Moraceae), Ascaris suum, Trichuris ovis
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efecto del extracto etanolico de Ficus Carica moraceae sobre la formacion de la larva 2 de ascaris suum y trichuris ovis en condiciones de laboratorio effect of the ethanol extract of Ficus Carica moraceae on the formation of ascaris suum and trichur
2015Co-Authors: Karen Siccha Aguilar, Victor Teran, Cesar A JaraAbstract:Parasitic helminth infections cause decrease in livestock production and, before the emergence of resistance to anthelmintic use of chemicals, the need to inquire about the antiparasitic capacity of plant extracts so far unused. In this research the effect of ethanol extract of the leaves of Ficus Carica (Moraceae) on the formation of the larva 2 (L2) of Ascaris suum and Trichuris ovis was assessed; for this purpose, fertile eggs, not embryonated, were obtained the uterus of pregnant females of both species, which were placed in Petri dishes, about thousand eggs per plate: three of which (E1, E2 and E3) with the extract, at the concentrations of 4000, 2000 and 1000 ppm and a fourth (C) with physiological saline (the experiment was done in duplicate). The Plates were maintained at room temperature (25-26 ° C) and every 48 hours microscopic observations were made to verify the formation of L2. At 20 days, 2.4% C without L2 eggs of A. suum and 2.3% of T. ovis was obtained, and E1 the highest percentage of eggs without L2 of both species were observed, 38.8% and 32.7%, respectively (p <0.05). It is concluded that the Ficus Carica-ethanol extract inhibits the formation of A. suum and T. ovis larva 2 at 20 days post treatment, under laboratory conditions.