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B Ollivier - One of the best experts on this subject based on the ideXlab platform.

  • biocorrosion of carbon steel alloys by an hydrogenotrophic sulfate reducing bacterium desulfovibrio capillatus isolated from a mexican oil Field separator
    Corrosion Science, 2006
    Co-Authors: E Miranda, M.j. Cano, M Bethencourt, F J Botana, J M Sanchezamaya, Alfonso Corzo, Garcia J De Lomas, M L Fardeau, B Ollivier
    Abstract:

    Abstract The hydrogenotrophic sulfate-reducing bacterium (SRB) Desulfovibrio capillatus (DSM14982T) was isolated from an oil Field Separator.with serious corrosion problems; this is the study of its role in the corrosion of carbon steels under anaerobic conditions. Immersion tests with two steel alloys, St-35.8 (typical carbon steel employed in European naval industry), and API-5XL52 (weathering alloy steel employed in Mexican oil industries) were performed. Total exposure was 45 days and different concentrations of thiosulfate as electron acceptor for bacterial growth were employed. The samples immersed in media with SRB undergo fast activation and numerous active sites form on the surface. Microscopic observations were made by environmental scanning electron microscopy (ESEM). Weight loss and electrochemical testing included open circuit potential (Ecorr), polarization resistance (Rp), electrochemical impedance spectroscopy (EIS) and electrochemical noise (EN) were measured with and without bacteria in the culture medium in order to determine corrosion rates and mechanisms. All electrochemical techniques have shown that after the end of the exponential phase the corrosion activity notably increased due to the high concentration of bacterial metabolites. Finally, the corrosion behavior of API-5XL52 was worse than St-35.8.

  • desulfovibrio capillatus sp nov a novel sulfate reducing bacterium isolated from an oil Field Separator.located in the gulf of mexico
    Anaerobe, 2003
    Co-Authors: Elizabeth Mirandatello, M L Fardeau, Luis Fernandez, Florina Ramirez, Jean Luc Cayol, Pierre Thomas, Jean Louis Garcia, B Ollivier
    Abstract:

    Abstract A new spirilloid sulfate-reducing bacterium designated strain MET2T (T=type strain), was isolated from a Mexican oil Field Separator. Electron microscopy revealed a Gram-negative cell wall consisting of a 150 nm thick undulating outer membrane. Strain MET2T appeared singly or in long chains and was actively motile with a corkscrew-like motion. The isolate grew optimally at 40°C, pH 7.4 and 3% NaCl in a medium containing lactate, thiosulfate and yeast extract. Sulfate, sulfite, thiosulfate, and elemental sulfur served as electron acceptors but not nitrate or fumarate. Lactate, pyruvate and H2 (with acetate as carbon source) were used as electron donors. Pyruvate was fermented. Desulfoviridin and cyt c were present. The G+C content of the DNA was 58.7 mol%. Phylogenetic analysis based on 16S rDNA sequencing showed that strain MET2T was a member of the genus Desulfovibrio with “D. gracilis” and D. longus being its closest relatives (similarities of 98.3% and 97.1%, respectively). However, DNA–DNA hybridization studies indicated poor homologies (values

M L Fardeau - One of the best experts on this subject based on the ideXlab platform.

  • biocorrosion of carbon steel alloys by an hydrogenotrophic sulfate reducing bacterium desulfovibrio capillatus isolated from a mexican oil Field separator
    Corrosion Science, 2006
    Co-Authors: E Miranda, M.j. Cano, M Bethencourt, F J Botana, J M Sanchezamaya, Alfonso Corzo, Garcia J De Lomas, M L Fardeau, B Ollivier
    Abstract:

    Abstract The hydrogenotrophic sulfate-reducing bacterium (SRB) Desulfovibrio capillatus (DSM14982T) was isolated from an oil Field Separator.with serious corrosion problems; this is the study of its role in the corrosion of carbon steels under anaerobic conditions. Immersion tests with two steel alloys, St-35.8 (typical carbon steel employed in European naval industry), and API-5XL52 (weathering alloy steel employed in Mexican oil industries) were performed. Total exposure was 45 days and different concentrations of thiosulfate as electron acceptor for bacterial growth were employed. The samples immersed in media with SRB undergo fast activation and numerous active sites form on the surface. Microscopic observations were made by environmental scanning electron microscopy (ESEM). Weight loss and electrochemical testing included open circuit potential (Ecorr), polarization resistance (Rp), electrochemical impedance spectroscopy (EIS) and electrochemical noise (EN) were measured with and without bacteria in the culture medium in order to determine corrosion rates and mechanisms. All electrochemical techniques have shown that after the end of the exponential phase the corrosion activity notably increased due to the high concentration of bacterial metabolites. Finally, the corrosion behavior of API-5XL52 was worse than St-35.8.

  • desulfovibrio capillatus sp nov a novel sulfate reducing bacterium isolated from an oil Field Separator.located in the gulf of mexico
    Anaerobe, 2003
    Co-Authors: Elizabeth Mirandatello, M L Fardeau, Luis Fernandez, Florina Ramirez, Jean Luc Cayol, Pierre Thomas, Jean Louis Garcia, B Ollivier
    Abstract:

    Abstract A new spirilloid sulfate-reducing bacterium designated strain MET2T (T=type strain), was isolated from a Mexican oil Field Separator. Electron microscopy revealed a Gram-negative cell wall consisting of a 150 nm thick undulating outer membrane. Strain MET2T appeared singly or in long chains and was actively motile with a corkscrew-like motion. The isolate grew optimally at 40°C, pH 7.4 and 3% NaCl in a medium containing lactate, thiosulfate and yeast extract. Sulfate, sulfite, thiosulfate, and elemental sulfur served as electron acceptors but not nitrate or fumarate. Lactate, pyruvate and H2 (with acetate as carbon source) were used as electron donors. Pyruvate was fermented. Desulfoviridin and cyt c were present. The G+C content of the DNA was 58.7 mol%. Phylogenetic analysis based on 16S rDNA sequencing showed that strain MET2T was a member of the genus Desulfovibrio with “D. gracilis” and D. longus being its closest relatives (similarities of 98.3% and 97.1%, respectively). However, DNA–DNA hybridization studies indicated poor homologies (values

Elizabeth Mirandatello - One of the best experts on this subject based on the ideXlab platform.

  • desulfovibrio capillatus sp nov a novel sulfate reducing bacterium isolated from an oil Field Separator.located in the gulf of mexico
    Anaerobe, 2003
    Co-Authors: Elizabeth Mirandatello, M L Fardeau, Luis Fernandez, Florina Ramirez, Jean Luc Cayol, Pierre Thomas, Jean Louis Garcia, B Ollivier
    Abstract:

    Abstract A new spirilloid sulfate-reducing bacterium designated strain MET2T (T=type strain), was isolated from a Mexican oil Field Separator. Electron microscopy revealed a Gram-negative cell wall consisting of a 150 nm thick undulating outer membrane. Strain MET2T appeared singly or in long chains and was actively motile with a corkscrew-like motion. The isolate grew optimally at 40°C, pH 7.4 and 3% NaCl in a medium containing lactate, thiosulfate and yeast extract. Sulfate, sulfite, thiosulfate, and elemental sulfur served as electron acceptors but not nitrate or fumarate. Lactate, pyruvate and H2 (with acetate as carbon source) were used as electron donors. Pyruvate was fermented. Desulfoviridin and cyt c were present. The G+C content of the DNA was 58.7 mol%. Phylogenetic analysis based on 16S rDNA sequencing showed that strain MET2T was a member of the genus Desulfovibrio with “D. gracilis” and D. longus being its closest relatives (similarities of 98.3% and 97.1%, respectively). However, DNA–DNA hybridization studies indicated poor homologies (values

Jean Louis Garcia - One of the best experts on this subject based on the ideXlab platform.

  • desulfovibrio capillatus sp nov a novel sulfate reducing bacterium isolated from an oil Field Separator.located in the gulf of mexico
    Anaerobe, 2003
    Co-Authors: Elizabeth Mirandatello, M L Fardeau, Luis Fernandez, Florina Ramirez, Jean Luc Cayol, Pierre Thomas, Jean Louis Garcia, B Ollivier
    Abstract:

    Abstract A new spirilloid sulfate-reducing bacterium designated strain MET2T (T=type strain), was isolated from a Mexican oil Field Separator. Electron microscopy revealed a Gram-negative cell wall consisting of a 150 nm thick undulating outer membrane. Strain MET2T appeared singly or in long chains and was actively motile with a corkscrew-like motion. The isolate grew optimally at 40°C, pH 7.4 and 3% NaCl in a medium containing lactate, thiosulfate and yeast extract. Sulfate, sulfite, thiosulfate, and elemental sulfur served as electron acceptors but not nitrate or fumarate. Lactate, pyruvate and H2 (with acetate as carbon source) were used as electron donors. Pyruvate was fermented. Desulfoviridin and cyt c were present. The G+C content of the DNA was 58.7 mol%. Phylogenetic analysis based on 16S rDNA sequencing showed that strain MET2T was a member of the genus Desulfovibrio with “D. gracilis” and D. longus being its closest relatives (similarities of 98.3% and 97.1%, respectively). However, DNA–DNA hybridization studies indicated poor homologies (values

Pierre Thomas - One of the best experts on this subject based on the ideXlab platform.

  • desulfovibrio capillatus sp nov a novel sulfate reducing bacterium isolated from an oil Field Separator.located in the gulf of mexico
    Anaerobe, 2003
    Co-Authors: Elizabeth Mirandatello, M L Fardeau, Luis Fernandez, Florina Ramirez, Jean Luc Cayol, Pierre Thomas, Jean Louis Garcia, B Ollivier
    Abstract:

    Abstract A new spirilloid sulfate-reducing bacterium designated strain MET2T (T=type strain), was isolated from a Mexican oil Field Separator. Electron microscopy revealed a Gram-negative cell wall consisting of a 150 nm thick undulating outer membrane. Strain MET2T appeared singly or in long chains and was actively motile with a corkscrew-like motion. The isolate grew optimally at 40°C, pH 7.4 and 3% NaCl in a medium containing lactate, thiosulfate and yeast extract. Sulfate, sulfite, thiosulfate, and elemental sulfur served as electron acceptors but not nitrate or fumarate. Lactate, pyruvate and H2 (with acetate as carbon source) were used as electron donors. Pyruvate was fermented. Desulfoviridin and cyt c were present. The G+C content of the DNA was 58.7 mol%. Phylogenetic analysis based on 16S rDNA sequencing showed that strain MET2T was a member of the genus Desulfovibrio with “D. gracilis” and D. longus being its closest relatives (similarities of 98.3% and 97.1%, respectively). However, DNA–DNA hybridization studies indicated poor homologies (values