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Engin Ulukaya - One of the best experts on this subject based on the ideXlab platform.
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parmelia sulcata taylor and usnea Filipendula stirt induce apoptosis like cell death and dna damage in cancer cells
Cell Proliferation, 2014Co-Authors: Ferda Ari, Seyhan Oran, Nazlihan Aztopal, S. Bozdemir, Serap Celikler, Sule Ozturk, Engin UlukayaAbstract:Objectives: Successful cancer treatments still require more compounds to be isolated from natural sources. Thus, we have investigated anti-proliferative/apoptotic effects of methanolic extracts of lichen species Parmelia sulcata Taylor and Usnea Filipendula Stirt on human lung cancer (A549, PC3), liver cancer (Hep3B) and rat glioma (C6) cells. Materials and methods: Anti-proliferative effects were monitored by MTT and adenosine triphosphate viability assays, while genotoxic activity was studied using the comet assay. Additionally, cell death mode and apoptosis assays (fluorescence staining, caspase-cleaved cytokeratin 18, caspase-3 activity and PARP cleavage) were performed. Results: Extracts produced anti-population growth effects in a dose-dependent manner (1.56–100 lg/ ml) by inducing apoptosis-like cell death. This resulted in the lines having the presence of pyknotic cell nuclei. In addition, significant increase in genetic damage in the cell lines was seen, indicating that DNA damage may have been responsible for apoptotic cell death. Conclusion: In this study, methanolic extracts of Parmelia sulcata and Usnea Filipendula induced apoptosis-like cell death by causing DNA damage, to cancer cells.
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Parmelia sulcata Taylor and Usnea Filipendula Stirt induce apoptosis‐like cell death and DNA damage in cancer cells
Cell proliferation, 2014Co-Authors: Ferda Ari, Seyhan Oran, Nazlihan Aztopal, S. Bozdemir, Serap Celikler, Sule Ozturk, Engin UlukayaAbstract:Objectives: Successful cancer treatments still require more compounds to be isolated from natural sources. Thus, we have investigated anti-proliferative/apoptotic effects of methanolic extracts of lichen species Parmelia sulcata Taylor and Usnea Filipendula Stirt on human lung cancer (A549, PC3), liver cancer (Hep3B) and rat glioma (C6) cells. Materials and methods: Anti-proliferative effects were monitored by MTT and adenosine triphosphate viability assays, while genotoxic activity was studied using the comet assay. Additionally, cell death mode and apoptosis assays (fluorescence staining, caspase-cleaved cytokeratin 18, caspase-3 activity and PARP cleavage) were performed. Results: Extracts produced anti-population growth effects in a dose-dependent manner (1.56–100 lg/ ml) by inducing apoptosis-like cell death. This resulted in the lines having the presence of pyknotic cell nuclei. In addition, significant increase in genetic damage in the cell lines was seen, indicating that DNA damage may have been responsible for apoptotic cell death. Conclusion: In this study, methanolic extracts of Parmelia sulcata and Usnea Filipendula induced apoptosis-like cell death by causing DNA damage, to cancer cells.
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Genotoxic, cytotoxic, and apoptotic effects of crude extract of Usnea Filipendula Stirt. in vitro
TURKISH JOURNAL OF BIOLOGY, 2014Co-Authors: Serap Çelikler Kasimoğullari, Seyhan Oran, Ferda Ari, Engin UlukayaAbstract:Lichens are recently receiving great attention because they have potential anticancer activity. Therefore, in this study, genotoxic and antigrowth properties of lichen species Usnea Filipendula Stirt. were tested against human breast cancer cell lines (MCF-7 and MDA-MB-231). Antigrowth effect was assayed by the MTT and ATP viability assays. Cell death modes (apoptosis/necrosis) were evaluated morphologically (fluorescence staining) and biochemically (caspase-cleaved cytokeratin 18, caspase-3 activity, and poly-(ADP-ribose) polymerase (PARP) cleavage). Genotoxic activity of U. Filipendula was determined by using micronucleus, chromosomal aberration, and comet assays in human lymphocyte culture. U. Filipendula inhibited growth in a dose-dependent manner and induced apoptosis by cleavage of PARP and induction of active caspase-3. It also showed genotoxic activity in doses (125 and 250 µg/mL) higher than that required for apoptosis. These results suggest that U. Filipendula may induce apoptotic cell death at lower doses, while it may be genotoxic at higher doses.
Ferda Ari - One of the best experts on this subject based on the ideXlab platform.
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Usnea Filipendula Induces Apoptosis in Human Colon Cancer Cell Lines
Indian Journal of Pharmaceutical Sciences, 2018Co-Authors: C. Disoma, Seyhan Oran, Merve Erkisa, I. Aloglou, E. Ulkaya, Ferda AriAbstract:Lichens are complex organisms living in a symbiotic relationship with fungi and algae have recently received special interest in cancer research. The cytotoxic activities of Usnea Filipendula Stirt. lichen extract was investigated on colon cancer cell lines, HCT-15 and HT-29. Sulforhodamine B and ATP cell viability tests were used to monitor cytotoxic activity. The mode of cell death (apoptosis/necrosis) was determined using caspase-cleaved cytokeratin 18 (M30), caspase-3/7 activity and fluorescence staining techniques that included, Annexin-V, Hoechst 33342 and propidium iodide. Usnea Filipendula showed dose and time-dependent antiproliferative effect in HCT-15 and HT-29 cells. The IC50 values in HCT-15 and HT-29 cells were 17.92 and 41.87 µg/ml, respectively. The extract induced apoptosis in both cell lines especially in HCT-15 cells in which caspase-3/7 activity was increased. Usnea Filipendula was cytotoxic to colon cancer HCT-15 and HT-29 cell lines by inducing early or late apoptosis as evidenced by translocation of phosphatidylserine, pyknotic nuclei and nuclear condensation. Further studies would help to understand the full potential of Usnea Filipendula as a novel anticancer therapy.
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parmelia sulcata taylor and usnea Filipendula stirt induce apoptosis like cell death and dna damage in cancer cells
Cell Proliferation, 2014Co-Authors: Ferda Ari, Seyhan Oran, Nazlihan Aztopal, S. Bozdemir, Serap Celikler, Sule Ozturk, Engin UlukayaAbstract:Objectives: Successful cancer treatments still require more compounds to be isolated from natural sources. Thus, we have investigated anti-proliferative/apoptotic effects of methanolic extracts of lichen species Parmelia sulcata Taylor and Usnea Filipendula Stirt on human lung cancer (A549, PC3), liver cancer (Hep3B) and rat glioma (C6) cells. Materials and methods: Anti-proliferative effects were monitored by MTT and adenosine triphosphate viability assays, while genotoxic activity was studied using the comet assay. Additionally, cell death mode and apoptosis assays (fluorescence staining, caspase-cleaved cytokeratin 18, caspase-3 activity and PARP cleavage) were performed. Results: Extracts produced anti-population growth effects in a dose-dependent manner (1.56–100 lg/ ml) by inducing apoptosis-like cell death. This resulted in the lines having the presence of pyknotic cell nuclei. In addition, significant increase in genetic damage in the cell lines was seen, indicating that DNA damage may have been responsible for apoptotic cell death. Conclusion: In this study, methanolic extracts of Parmelia sulcata and Usnea Filipendula induced apoptosis-like cell death by causing DNA damage, to cancer cells.
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Parmelia sulcata Taylor and Usnea Filipendula Stirt induce apoptosis‐like cell death and DNA damage in cancer cells
Cell proliferation, 2014Co-Authors: Ferda Ari, Seyhan Oran, Nazlihan Aztopal, S. Bozdemir, Serap Celikler, Sule Ozturk, Engin UlukayaAbstract:Objectives: Successful cancer treatments still require more compounds to be isolated from natural sources. Thus, we have investigated anti-proliferative/apoptotic effects of methanolic extracts of lichen species Parmelia sulcata Taylor and Usnea Filipendula Stirt on human lung cancer (A549, PC3), liver cancer (Hep3B) and rat glioma (C6) cells. Materials and methods: Anti-proliferative effects were monitored by MTT and adenosine triphosphate viability assays, while genotoxic activity was studied using the comet assay. Additionally, cell death mode and apoptosis assays (fluorescence staining, caspase-cleaved cytokeratin 18, caspase-3 activity and PARP cleavage) were performed. Results: Extracts produced anti-population growth effects in a dose-dependent manner (1.56–100 lg/ ml) by inducing apoptosis-like cell death. This resulted in the lines having the presence of pyknotic cell nuclei. In addition, significant increase in genetic damage in the cell lines was seen, indicating that DNA damage may have been responsible for apoptotic cell death. Conclusion: In this study, methanolic extracts of Parmelia sulcata and Usnea Filipendula induced apoptosis-like cell death by causing DNA damage, to cancer cells.
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Genotoxic, cytotoxic, and apoptotic effects of crude extract of Usnea Filipendula Stirt. in vitro
TURKISH JOURNAL OF BIOLOGY, 2014Co-Authors: Serap Çelikler Kasimoğullari, Seyhan Oran, Ferda Ari, Engin UlukayaAbstract:Lichens are recently receiving great attention because they have potential anticancer activity. Therefore, in this study, genotoxic and antigrowth properties of lichen species Usnea Filipendula Stirt. were tested against human breast cancer cell lines (MCF-7 and MDA-MB-231). Antigrowth effect was assayed by the MTT and ATP viability assays. Cell death modes (apoptosis/necrosis) were evaluated morphologically (fluorescence staining) and biochemically (caspase-cleaved cytokeratin 18, caspase-3 activity, and poly-(ADP-ribose) polymerase (PARP) cleavage). Genotoxic activity of U. Filipendula was determined by using micronucleus, chromosomal aberration, and comet assays in human lymphocyte culture. U. Filipendula inhibited growth in a dose-dependent manner and induced apoptosis by cleavage of PARP and induction of active caspase-3. It also showed genotoxic activity in doses (125 and 250 µg/mL) higher than that required for apoptosis. These results suggest that U. Filipendula may induce apoptotic cell death at lower doses, while it may be genotoxic at higher doses.
Edemar Roberto Andreatta - One of the best experts on this subject based on the ideXlab platform.
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Brown seaweeds as feed additive for white-leg shrimp: effects on thermal stress resistance, midgut microbiology, and immunology
Journal of Applied Phycology, 2017Co-Authors: Delano Dias Schleder, Juliana Ribeiro Rosa, Ariane Martins Guimarães, Leila Hayashi, Felipe Nascimento Vieira, Walter Quadros Seiffert, Fernanda Ramlov, Marcelo Maraschin, Edemar Roberto AndreattaAbstract:This study evaluated the midgut microbiology, hemato-immunological parameters and the resistance to thermal shock of the white-leg shrimp Litopenaeus vannamei fed with diets containing 0.5, 2, and 4% of the brown seaweeds Undaria pinnatifida and Sargassum Filipendula. Shrimp were reared in clear seawater for 15 days in seven 800-L tanks with 100 shrimp (5.9 ± 0.2 g), under constant aeration and temperature (29 °C), with 80% daily water exchange. Animals were fed four times per day (6% of biomass) during the experimental period. At day 16, bacterial count of midgut and hemato-immunological parameters were assessed before the thermal shock; during thermal shock, shrimp were transferred from seawater at 29 to 12.5 °C for 1 h, and then back to 29 °C, and kept for 48 h to estimate the cumulative mortality. Animals fed with 4% of U. pinnatifida had lower Vibrio spp. quantity in the midgut and showed an overall improvement of evaluated hemato-immunological parameters; however, only phenoloxidase activity was statistically significant. Those fed with 0.5 and 2% of S. Filipendula showed lower cumulative mortality after thermal shock, in comparison with control and those fed with 4% of this species. Increasing levels of U. pinnatifida had negative effects on thermal shock resistance. In conclusion, U. pinnatifida enhances shrimp immunity and specifically inhibit Vibrio spp., while S. Filipendula increases shrimp resistance to thermal variation.
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Thermal resistance of Pacific white shrimp fed Sargassum Filipendula: A MALDI-TOF mass spectrometry approach
Aquaculture, 2017Co-Authors: Delano Dias Schleder, Edemar Roberto Andreatta, Martina Blank, Luiz Guilherme Buglione Peruch, Felipe Do Nascimento Vieira, Leila HayashiAbstract:Abstract Pacific white shrimp, Litopenaeus vannamei, is a tropical species known to be intolerant to cold. In our earlier work, increased thermal shock resistance caused by Sargassum Filipendula raised unanswered questions about the mechanisms responsible for this resistance. Therefore, in the present work, we used MALDI-TOF mass spectrometry (MS) to assess protein and lipid profiles of intact and lysed hemocytes from Pacific white shrimp fed 0.5% of S. Filipendula and submitted to thermal shock. To accomplish this, shrimp (13.0 ± 0.4 g) were reared during 15 days in 800 L tanks (35 shrimps tank− 1), in duplicate, under constant aeration and temperature (28.5 °C), 100% of daily water exchange and feeding four times day− 1. At day 16, animals were submitted to thermal shock by passing from seawater at 28.5 °C to 11.5 °C, where they remained for 1 h, and then returning to 28.5 °C. Mortality was monitored during 24 h. Hemolymph of 10 animals per treatment (control and 0.5% S. Filipendula) was collected before and 15 min after thermal shock for MALDI-TOF MS analyses. Hemocytes were processed in both intact and lysed cells. Samples were mixed (1:1) with 2,5-dihydroxy-benzoic acid matrix for lipid analysis and sinapinic acid for protein. Shrimp fed 0.5% S. Filipendula showed higher signal intensity of phosphatidylcholines with unsaturated fatty acids and lower signal intensity of lysophosphatidylcholines, after thermal shock. For proteins, S. Filipendula increased signals that could be associated with antimicrobial peptides and histone H2A, whereas reduced signals were related to Cytochrome c oxidase subunits. Based on these results, it can be concluded that S. Filipendula increased membrane fluidity and antimicrobial defense, diminished apoptotic signaling, and modulated DNA regulation, lipid and energy metabolism of shrimp hemocytes. These mechanisms could explain, at least in part, the higher thermal shock resistance of these shrimp fed a diet with 0.5% S. Filipendula.
Delano Dias Schleder - One of the best experts on this subject based on the ideXlab platform.
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Brown seaweeds as feed additive for white-leg shrimp: effects on thermal stress resistance, midgut microbiology, and immunology
Journal of Applied Phycology, 2017Co-Authors: Delano Dias Schleder, Juliana Ribeiro Rosa, Ariane Martins Guimarães, Leila Hayashi, Felipe Nascimento Vieira, Walter Quadros Seiffert, Fernanda Ramlov, Marcelo Maraschin, Edemar Roberto AndreattaAbstract:This study evaluated the midgut microbiology, hemato-immunological parameters and the resistance to thermal shock of the white-leg shrimp Litopenaeus vannamei fed with diets containing 0.5, 2, and 4% of the brown seaweeds Undaria pinnatifida and Sargassum Filipendula. Shrimp were reared in clear seawater for 15 days in seven 800-L tanks with 100 shrimp (5.9 ± 0.2 g), under constant aeration and temperature (29 °C), with 80% daily water exchange. Animals were fed four times per day (6% of biomass) during the experimental period. At day 16, bacterial count of midgut and hemato-immunological parameters were assessed before the thermal shock; during thermal shock, shrimp were transferred from seawater at 29 to 12.5 °C for 1 h, and then back to 29 °C, and kept for 48 h to estimate the cumulative mortality. Animals fed with 4% of U. pinnatifida had lower Vibrio spp. quantity in the midgut and showed an overall improvement of evaluated hemato-immunological parameters; however, only phenoloxidase activity was statistically significant. Those fed with 0.5 and 2% of S. Filipendula showed lower cumulative mortality after thermal shock, in comparison with control and those fed with 4% of this species. Increasing levels of U. pinnatifida had negative effects on thermal shock resistance. In conclusion, U. pinnatifida enhances shrimp immunity and specifically inhibit Vibrio spp., while S. Filipendula increases shrimp resistance to thermal variation.
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Thermal resistance of Pacific white shrimp fed Sargassum Filipendula: A MALDI-TOF mass spectrometry approach
Aquaculture, 2017Co-Authors: Delano Dias Schleder, Edemar Roberto Andreatta, Martina Blank, Luiz Guilherme Buglione Peruch, Felipe Do Nascimento Vieira, Leila HayashiAbstract:Abstract Pacific white shrimp, Litopenaeus vannamei, is a tropical species known to be intolerant to cold. In our earlier work, increased thermal shock resistance caused by Sargassum Filipendula raised unanswered questions about the mechanisms responsible for this resistance. Therefore, in the present work, we used MALDI-TOF mass spectrometry (MS) to assess protein and lipid profiles of intact and lysed hemocytes from Pacific white shrimp fed 0.5% of S. Filipendula and submitted to thermal shock. To accomplish this, shrimp (13.0 ± 0.4 g) were reared during 15 days in 800 L tanks (35 shrimps tank− 1), in duplicate, under constant aeration and temperature (28.5 °C), 100% of daily water exchange and feeding four times day− 1. At day 16, animals were submitted to thermal shock by passing from seawater at 28.5 °C to 11.5 °C, where they remained for 1 h, and then returning to 28.5 °C. Mortality was monitored during 24 h. Hemolymph of 10 animals per treatment (control and 0.5% S. Filipendula) was collected before and 15 min after thermal shock for MALDI-TOF MS analyses. Hemocytes were processed in both intact and lysed cells. Samples were mixed (1:1) with 2,5-dihydroxy-benzoic acid matrix for lipid analysis and sinapinic acid for protein. Shrimp fed 0.5% S. Filipendula showed higher signal intensity of phosphatidylcholines with unsaturated fatty acids and lower signal intensity of lysophosphatidylcholines, after thermal shock. For proteins, S. Filipendula increased signals that could be associated with antimicrobial peptides and histone H2A, whereas reduced signals were related to Cytochrome c oxidase subunits. Based on these results, it can be concluded that S. Filipendula increased membrane fluidity and antimicrobial defense, diminished apoptotic signaling, and modulated DNA regulation, lipid and energy metabolism of shrimp hemocytes. These mechanisms could explain, at least in part, the higher thermal shock resistance of these shrimp fed a diet with 0.5% S. Filipendula.
Leila Hayashi - One of the best experts on this subject based on the ideXlab platform.
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Brown seaweeds as feed additive for white-leg shrimp: effects on thermal stress resistance, midgut microbiology, and immunology
Journal of Applied Phycology, 2017Co-Authors: Delano Dias Schleder, Juliana Ribeiro Rosa, Ariane Martins Guimarães, Leila Hayashi, Felipe Nascimento Vieira, Walter Quadros Seiffert, Fernanda Ramlov, Marcelo Maraschin, Edemar Roberto AndreattaAbstract:This study evaluated the midgut microbiology, hemato-immunological parameters and the resistance to thermal shock of the white-leg shrimp Litopenaeus vannamei fed with diets containing 0.5, 2, and 4% of the brown seaweeds Undaria pinnatifida and Sargassum Filipendula. Shrimp were reared in clear seawater for 15 days in seven 800-L tanks with 100 shrimp (5.9 ± 0.2 g), under constant aeration and temperature (29 °C), with 80% daily water exchange. Animals were fed four times per day (6% of biomass) during the experimental period. At day 16, bacterial count of midgut and hemato-immunological parameters were assessed before the thermal shock; during thermal shock, shrimp were transferred from seawater at 29 to 12.5 °C for 1 h, and then back to 29 °C, and kept for 48 h to estimate the cumulative mortality. Animals fed with 4% of U. pinnatifida had lower Vibrio spp. quantity in the midgut and showed an overall improvement of evaluated hemato-immunological parameters; however, only phenoloxidase activity was statistically significant. Those fed with 0.5 and 2% of S. Filipendula showed lower cumulative mortality after thermal shock, in comparison with control and those fed with 4% of this species. Increasing levels of U. pinnatifida had negative effects on thermal shock resistance. In conclusion, U. pinnatifida enhances shrimp immunity and specifically inhibit Vibrio spp., while S. Filipendula increases shrimp resistance to thermal variation.
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Thermal resistance of Pacific white shrimp fed Sargassum Filipendula: A MALDI-TOF mass spectrometry approach
Aquaculture, 2017Co-Authors: Delano Dias Schleder, Edemar Roberto Andreatta, Martina Blank, Luiz Guilherme Buglione Peruch, Felipe Do Nascimento Vieira, Leila HayashiAbstract:Abstract Pacific white shrimp, Litopenaeus vannamei, is a tropical species known to be intolerant to cold. In our earlier work, increased thermal shock resistance caused by Sargassum Filipendula raised unanswered questions about the mechanisms responsible for this resistance. Therefore, in the present work, we used MALDI-TOF mass spectrometry (MS) to assess protein and lipid profiles of intact and lysed hemocytes from Pacific white shrimp fed 0.5% of S. Filipendula and submitted to thermal shock. To accomplish this, shrimp (13.0 ± 0.4 g) were reared during 15 days in 800 L tanks (35 shrimps tank− 1), in duplicate, under constant aeration and temperature (28.5 °C), 100% of daily water exchange and feeding four times day− 1. At day 16, animals were submitted to thermal shock by passing from seawater at 28.5 °C to 11.5 °C, where they remained for 1 h, and then returning to 28.5 °C. Mortality was monitored during 24 h. Hemolymph of 10 animals per treatment (control and 0.5% S. Filipendula) was collected before and 15 min after thermal shock for MALDI-TOF MS analyses. Hemocytes were processed in both intact and lysed cells. Samples were mixed (1:1) with 2,5-dihydroxy-benzoic acid matrix for lipid analysis and sinapinic acid for protein. Shrimp fed 0.5% S. Filipendula showed higher signal intensity of phosphatidylcholines with unsaturated fatty acids and lower signal intensity of lysophosphatidylcholines, after thermal shock. For proteins, S. Filipendula increased signals that could be associated with antimicrobial peptides and histone H2A, whereas reduced signals were related to Cytochrome c oxidase subunits. Based on these results, it can be concluded that S. Filipendula increased membrane fluidity and antimicrobial defense, diminished apoptotic signaling, and modulated DNA regulation, lipid and energy metabolism of shrimp hemocytes. These mechanisms could explain, at least in part, the higher thermal shock resistance of these shrimp fed a diet with 0.5% S. Filipendula.