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A Goksøyr - One of the best experts on this subject based on the ideXlab platform.
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Fish Model for assessing the in vivo estrogenic potency of the mycotoxin zearalenone and its metabolites.
Science of The Total Environment, 1999Co-Authors: A Arukwe, T Grotmol, T B Haugen, F R Knudsen, A GoksøyrAbstract:The in vivo estrogenic potency of zearalenone (ZEA), a mycotoxin produced by different strains of Fusarium fungi, and its metabolites (α- and β-zearalenol), have been studied in Fish. Estrogenicity was evaluated using an in vitro competitive receptor binding assay and in vivo induction of vitellogenesis and zonagenesis, two estrogen receptor (ER)-mediated responses that are integral aspects of Fish oogenesis. The ER binding affinities of α-zearalenol and ZEA in rainbow trout (Oncorhynchus mykiss) were approximately 1/150 and 1/300 to that of estradiol, respectively. Juvenile salmon (Salmo salar) were exposed to a single intraperitoneal injection of ZEA, α-zearalenol and β-zearalenol (each at 1 and 10 mg/kg) and compared to Fish injected with estradiol-17β (E2; 5 mg/kg) and controls. Using indirect enzyme-linked immunosorbent assay (ELISA) with homologous antibodies, a dose-dependent induction of vitellogenin (Vtg) and eggshell zona radiata proteins (Zr-proteins) were observed 7 days after exposure to ZEA and α-zearalenol. β-Zearalenol did not elevate plasma Vtg levels, but a non-significant elevation of plasma Zr-proteins levels was observed at the highest dose (10 mg/kg). Generally, α-zearalenol and ZEA possess estrogenic potencies that are approximately 50% compared to that of E2, and their order of estrogenic potency (in both in vitro receptor competitive binding and in vivo induction of Vtg and Zr-proteins levels) is: α-zearalenol>ZEA>β-zearalenol. Our results show that blood plasma analysis of Vtg and Zr-proteins levels provides a suitable in vivo Fish Model for assessing the estrogenic potencies of ZEA and its metabolites.
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Fish Model for assessing the in vivo estrogenic potency of the mycotoxin zearalenone and its metabolites.
Science of The Total Environment, 1999Co-Authors: A Arukwe, T Grotmol, T B Haugen, F R Knudsen, A GoksøyrAbstract:The in vivo estrogenic potency of zearalenone (ZEA), a mycotoxin produced by different strains of Fusarium fungi, and its metabolites (alpha- and beta-zearalenol), have been studied in Fish. Estrogenicity was evaluated using an in vitro competitive receptor binding assay and in vivo induction of vitellogenesis and zonagenesis, two estrogen receptor (ER)-mediated responses that are integral aspects of Fish oogenesis. The ER binding affinities of alpha-zearalenol and ZEA in rainbow trout (Oncorhynchus mykiss) were approximately 1/150 and 1/300 to that of estradiol, respectively. Juvenile salmon (Salmo salar) were exposed to a single intraperitoneal injection of ZEA, alpha-zearalenol and beta-zearalenol (each at 1 and 10 mg/kg) and compared to Fish injected with estradiol-17 beta (E2; 5 mg/kg) and controls. Using indirect enzyme-linked immunosorbent assay (ELISA) with homologous antibodies, a dose-dependent induction of vitellogenin (Vtg) and eggshell zona radiata proteins (Zr-proteins) were observed 7 days after exposure to ZEA and alpha-zearalenol. beta-Zearalenol did not elevate plasma Vtg levels, but a non-significant elevation of plasma Zr-proteins levels was observed at the highest dose (10 mg/kg). Generally, alpha-zearalenol and ZEA possess estrogenic potencies that are approximately 50% compared to that of E2, and their order of estrogenic potency (in both in vitro receptor competitive binding and in vivo induction of Vtg and Zr-proteins levels) is: alpha-zearalenol > ZEA > beta-zearalenol. Our results show that blood plasma analysis of Vtg and Zr-proteins levels provides a suitable in vivo Fish Model for assessing the estrogenic potencies of ZEA and its metabolites.
Wei Weng - One of the best experts on this subject based on the ideXlab platform.
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A small-Fish Model for behavioral-toxicological screening of new antimalarial drugs: a comparison between erythro- and threo-mefloquine.
BMC research notes, 2015Co-Authors: Hans Maaswinkel, Liqun Zhu, Wei WengAbstract:Background New antimalarial drugs need to be developed because over time resistance against the existing drugs develops. Furthermore, some of the drugs have severe side effects. Here we describe a behavioral small-Fish Model for early detection of neurotoxic effects of new drugs. As case example we compare the effects of two mefloquine diastereomers on the behavior of goldFish using an automated 3D tracking system.
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A small-Fish Model for behavioral-toxicological screening of new antimalarial drugs: a comparison between erythro- and threo-mefloquine
BMC Research Notes, 2015Co-Authors: Hans Maaswinkel, Liqun Zhu, Wei WengAbstract:Background New antimalarial drugs need to be developed because over time resistance against the existing drugs develops. Furthermore, some of the drugs have severe side effects. Here we describe a behavioral small-Fish Model for early detection of neurotoxic effects of new drugs. As case example we compare the effects of two mefloquine diastereomers on the behavior of goldFish using an automated 3D tracking system. Findings In a preliminary experiment, the overall toxic effects in terms of motor and respiratory impairments were determined during a 3-hour exposure to the drugs at relatively high doses (21.5 and 43 mgL). In the second experiment, behavioral testing was performed 24 h after a 3.5-h drug exposure to a low dose (14.25 mgL) of either drug. For the two high doses, erythro-mefloquine resulted in severe motor problems and respiratory problems occurred. In goldFish treated with threo-mefloquine, at 43 mgL the motor/respiratory impairments were less severe and at 21.5 mgL no such problems were observed. For the lower dose (14.25 mgL), erythro-mefloquine reduced locomotion. There was also a tendency for increased freezing, and the preference for quadrant two of the observation container was increased. No behavioral effects of threo-mefloquine were found. Conclusions The results demonstrate that in goldFish exposed to the drugs dissolved in the water, threo-mefloquine has less severe toxic effects as compared to erythro-mefloquine. These findings are consistent with other studies and support the usefulness of the small-Fish Model for predicting adverse effects of new antimalarial drugs during the initial phases of drug development.
Antoni Ibarz - One of the best experts on this subject based on the ideXlab platform.
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Chronic Cold Stress Alters the Skin Mucus Interactome in a Temperate Fish Model.
Frontiers in physiology, 2019Co-Authors: Ignasi Sanahuja, Laura Fernández-alacid, Sergio Sánchez-nuño, Borja Ordóñez-grande, Antoni IbarzAbstract:Temperate Fish are particularly sensitive to low temperatures, especially in the northern Mediterranean area, where the cold season decreases Fish-farm production and affects Fish health. Recent studies have suggested that the skin mucus participates in overall Fish defence and welfare, and therefore propose it as a target for non-invasive studies of Fish status. Here, we determine the mucus interactome of differentially expressed proteins in a temperate Fish Model, gilthead sea bream (Sparus aurata), after chronic exposure to low temperatures (seven weeks at 14oC). The differentially expressed proteins were obtained by 2D-PAGE of mucus soluble proteins and further assessed by STRING analyses of the functional interactome based on protein-protein interactions. Complementarily, we determined mucus metabolites, glucose and protein, as well as enzymes involved in innate defence mechanisms such as total protease and esterase. The cold mucus interactome revealed the presence of several subsets of proteins corresponding to Gene Ontology groups. “Response to stress” formed the central core of the cold interactome, with up-regulation of proteins such as heat shock proteins (HSPs) and transferrin; and down-regulation of proteins with metabolic activity. In accordance with the low temperatures, all proteins clustered in the “Single-organism metabolic process” group were down-regulated in response to cold, evidencing depressed skin metabolism. An interactome subset of “Interspecies interaction between species” grouped together several up-regulated mucus proteins that participate in bacterial adhesion, colonization and entry, such as HSP70, lectin-2, ribosomal proteins, and cytokeratin-8, septin and plakins. Furthermore, cold mucus showed lower levels of soluble glucose and no adaptation response in total protease or esterase activity. Using zymography, we detected the up-regulation of metalloprotease-like activity, together with a number of fragments or cleaved keratin forms which may present antimicrobial activity. All these results evidence a partial loss of mucus functionality under chronic exposure to low temperatures which would affect Fish welfare during the natural cold season under farm conditions.
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Chronic Cold Stress Alters the Skin Mucus Interactome in a Temperate Fish Model
Frontiers Media S.A., 2019Co-Authors: Ignasi Sanahuja, Laura Fernández-alacid, Sergio Sánchez-nuño, Borja Ordóñez-grande, Antoni IbarzAbstract:Temperate Fish are particularly sensitive to low temperatures, especially in the northern Mediterranean area, where the cold season decreases Fish-farm production and affects Fish health. Recent studies have suggested that the skin mucus participates in overall Fish defense and welfare, and therefore propose it as a target for non-invasive studies of Fish status. Here, we determine the mucus interactome of differentially expressed proteins in a temperate Fish Model, gilthead sea bream (Sparus aurata), after chronic exposure to low temperatures (7 weeks at 14°C). The differentially expressed proteins were obtained by 2D-PAGE of mucus soluble proteins and further assessed by STRING analyses of the functional interactome based on protein-protein interactions. Complementarily, we determined mucus metabolites, glucose, and protein, as well as enzymes involved in innate defense mechanisms, such as total protease and esterase. The cold mucus interactome revealed the presence of several subsets of proteins corresponding to Gene Ontology groups. “Response to stress” formed the central core of the cold interactome, with up-regulation of proteins, such as heat shock proteins (HSPs) and transferrin; and down-regulation of proteins with metabolic activity. In accordance with the low temperatures, all proteins clustered in the “Single-organism metabolic process” group were down-regulated in response to cold, evidencing depressed skin metabolism. An interactome subset of “Interspecies interaction between species” grouped together several up-regulated mucus proteins that participate in bacterial adhesion, colonization, and entry, such as HSP70, lectin-2, ribosomal proteins, and cytokeratin-8, septin, and plakins. Furthermore, cold mucus showed lower levels of soluble glucose and no adaptation response in total protease or esterase activity. Using zymography, we detected the up-regulation of metalloprotease-like activity, together with a number of fragments or cleaved keratin forms which may present antimicrobial activity. All these results evidence a partial loss of mucus functionality under chronic exposure to low temperatures which would affect Fish welfare during the natural cold season under farm conditions
A Arukwe - One of the best experts on this subject based on the ideXlab platform.
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Fish Model for assessing the in vivo estrogenic potency of the mycotoxin zearalenone and its metabolites.
Science of The Total Environment, 1999Co-Authors: A Arukwe, T Grotmol, T B Haugen, F R Knudsen, A GoksøyrAbstract:The in vivo estrogenic potency of zearalenone (ZEA), a mycotoxin produced by different strains of Fusarium fungi, and its metabolites (α- and β-zearalenol), have been studied in Fish. Estrogenicity was evaluated using an in vitro competitive receptor binding assay and in vivo induction of vitellogenesis and zonagenesis, two estrogen receptor (ER)-mediated responses that are integral aspects of Fish oogenesis. The ER binding affinities of α-zearalenol and ZEA in rainbow trout (Oncorhynchus mykiss) were approximately 1/150 and 1/300 to that of estradiol, respectively. Juvenile salmon (Salmo salar) were exposed to a single intraperitoneal injection of ZEA, α-zearalenol and β-zearalenol (each at 1 and 10 mg/kg) and compared to Fish injected with estradiol-17β (E2; 5 mg/kg) and controls. Using indirect enzyme-linked immunosorbent assay (ELISA) with homologous antibodies, a dose-dependent induction of vitellogenin (Vtg) and eggshell zona radiata proteins (Zr-proteins) were observed 7 days after exposure to ZEA and α-zearalenol. β-Zearalenol did not elevate plasma Vtg levels, but a non-significant elevation of plasma Zr-proteins levels was observed at the highest dose (10 mg/kg). Generally, α-zearalenol and ZEA possess estrogenic potencies that are approximately 50% compared to that of E2, and their order of estrogenic potency (in both in vitro receptor competitive binding and in vivo induction of Vtg and Zr-proteins levels) is: α-zearalenol>ZEA>β-zearalenol. Our results show that blood plasma analysis of Vtg and Zr-proteins levels provides a suitable in vivo Fish Model for assessing the estrogenic potencies of ZEA and its metabolites.
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Fish Model for assessing the in vivo estrogenic potency of the mycotoxin zearalenone and its metabolites.
Science of The Total Environment, 1999Co-Authors: A Arukwe, T Grotmol, T B Haugen, F R Knudsen, A GoksøyrAbstract:The in vivo estrogenic potency of zearalenone (ZEA), a mycotoxin produced by different strains of Fusarium fungi, and its metabolites (alpha- and beta-zearalenol), have been studied in Fish. Estrogenicity was evaluated using an in vitro competitive receptor binding assay and in vivo induction of vitellogenesis and zonagenesis, two estrogen receptor (ER)-mediated responses that are integral aspects of Fish oogenesis. The ER binding affinities of alpha-zearalenol and ZEA in rainbow trout (Oncorhynchus mykiss) were approximately 1/150 and 1/300 to that of estradiol, respectively. Juvenile salmon (Salmo salar) were exposed to a single intraperitoneal injection of ZEA, alpha-zearalenol and beta-zearalenol (each at 1 and 10 mg/kg) and compared to Fish injected with estradiol-17 beta (E2; 5 mg/kg) and controls. Using indirect enzyme-linked immunosorbent assay (ELISA) with homologous antibodies, a dose-dependent induction of vitellogenin (Vtg) and eggshell zona radiata proteins (Zr-proteins) were observed 7 days after exposure to ZEA and alpha-zearalenol. beta-Zearalenol did not elevate plasma Vtg levels, but a non-significant elevation of plasma Zr-proteins levels was observed at the highest dose (10 mg/kg). Generally, alpha-zearalenol and ZEA possess estrogenic potencies that are approximately 50% compared to that of E2, and their order of estrogenic potency (in both in vitro receptor competitive binding and in vivo induction of Vtg and Zr-proteins levels) is: alpha-zearalenol > ZEA > beta-zearalenol. Our results show that blood plasma analysis of Vtg and Zr-proteins levels provides a suitable in vivo Fish Model for assessing the estrogenic potencies of ZEA and its metabolites.
Hans Maaswinkel - One of the best experts on this subject based on the ideXlab platform.
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A small-Fish Model for behavioral-toxicological screening of new antimalarial drugs: a comparison between erythro- and threo-mefloquine.
BMC research notes, 2015Co-Authors: Hans Maaswinkel, Liqun Zhu, Wei WengAbstract:Background New antimalarial drugs need to be developed because over time resistance against the existing drugs develops. Furthermore, some of the drugs have severe side effects. Here we describe a behavioral small-Fish Model for early detection of neurotoxic effects of new drugs. As case example we compare the effects of two mefloquine diastereomers on the behavior of goldFish using an automated 3D tracking system.
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A small-Fish Model for behavioral-toxicological screening of new antimalarial drugs: a comparison between erythro- and threo-mefloquine
BMC Research Notes, 2015Co-Authors: Hans Maaswinkel, Liqun Zhu, Wei WengAbstract:Background New antimalarial drugs need to be developed because over time resistance against the existing drugs develops. Furthermore, some of the drugs have severe side effects. Here we describe a behavioral small-Fish Model for early detection of neurotoxic effects of new drugs. As case example we compare the effects of two mefloquine diastereomers on the behavior of goldFish using an automated 3D tracking system. Findings In a preliminary experiment, the overall toxic effects in terms of motor and respiratory impairments were determined during a 3-hour exposure to the drugs at relatively high doses (21.5 and 43 mgL). In the second experiment, behavioral testing was performed 24 h after a 3.5-h drug exposure to a low dose (14.25 mgL) of either drug. For the two high doses, erythro-mefloquine resulted in severe motor problems and respiratory problems occurred. In goldFish treated with threo-mefloquine, at 43 mgL the motor/respiratory impairments were less severe and at 21.5 mgL no such problems were observed. For the lower dose (14.25 mgL), erythro-mefloquine reduced locomotion. There was also a tendency for increased freezing, and the preference for quadrant two of the observation container was increased. No behavioral effects of threo-mefloquine were found. Conclusions The results demonstrate that in goldFish exposed to the drugs dissolved in the water, threo-mefloquine has less severe toxic effects as compared to erythro-mefloquine. These findings are consistent with other studies and support the usefulness of the small-Fish Model for predicting adverse effects of new antimalarial drugs during the initial phases of drug development.