The Experts below are selected from a list of 72 Experts worldwide ranked by ideXlab platform

Stephen A Douglas - One of the best experts on this subject based on the ideXlab platform.

  • human urotensin ii as a novel cardiovascular target heart of the matter or simply a Fishy tail
    Current Opinion in Pharmacology, 2003
    Co-Authors: Stephen A Douglas
    Abstract:

    Abstract Urotensin-II (U-II), originally identified as a Fish neuroPeptide, exerts a broad spectrum of biological actions in mammals: responses that influence cardiorenal, pulmonary (bronchoconstriction), central nervous system (locomotion) and endocrine (thyroid-stimulating hormone, prolactin and insulin secretion) function. Because the U-II isoPeptide family is highly conserved across species, both amongst invertebrates and vertebrates, it has been inferred that U-II and its G-protein-coupled receptor, UT, play a seminal role in the physiological regulation of major mammalian organ systems, most notably within the cardiovasculature. However, despite the evolutionary conservation of U-II, the (patho)physiological significance of this ‘somatostatin-like’ Peptide remains ambiguous. Can the identification of a Fish Peptide as a ligand for an ‘orphan’ mammalian G-protein-coupled receptor really tell us something about human physiology? Emerging preclinical and clinical data suggest that it might.

  • Human urotensin-II as a novel cardiovascular target: 'heart' of the matter or simply a Fishy 'tail'?
    Current opinion in pharmacology, 2003
    Co-Authors: Stephen A Douglas
    Abstract:

    Abstract Urotensin-II (U-II), originally identified as a Fish neuroPeptide, exerts a broad spectrum of biological actions in mammals: responses that influence cardiorenal, pulmonary (bronchoconstriction), central nervous system (locomotion) and endocrine (thyroid-stimulating hormone, prolactin and insulin secretion) function. Because the U-II isoPeptide family is highly conserved across species, both amongst invertebrates and vertebrates, it has been inferred that U-II and its G-protein-coupled receptor, UT, play a seminal role in the physiological regulation of major mammalian organ systems, most notably within the cardiovasculature. However, despite the evolutionary conservation of U-II, the (patho)physiological significance of this ‘somatostatin-like’ Peptide remains ambiguous. Can the identification of a Fish Peptide as a ligand for an ‘orphan’ mammalian G-protein-coupled receptor really tell us something about human physiology? Emerging preclinical and clinical data suggest that it might.

Gerhard Haase - One of the best experts on this subject based on the ideXlab platform.

  • Rapid detection of Streptococcus agalactiae from swabs by Peptide nucleic acid fluorescence in situ hybridization.
    Journal of Medical Microbiology, 2009
    Co-Authors: Heidrun Peltroche-llacsahuanga, Mark J. Fiandaca, Sabine Von Oy, Rudolf Lütticken, Gerhard Haase
    Abstract:

    The applicability of the PNA Fish (Peptide nucleic acid fluorescence in situ hybridization) method for detection of Streptococcus agalactiae [group B streptococci (GBS)] from swab samples was evaluated. Three swab-sample-processing protocols with different time-to-result (TTR) values were compared: (i) direct smearing of fresh swabs onto microscope slides (n=153, TTR 2.5 h), (ii) further extraction and concentration of cells from these same swabs (n=153, TTR 2.7 h), and (iii) short-term LIM broth enrichment culture incubation (7 h, 37 °C) of fresh swabs (n=120, TTR 9.5 h). The sensitivity, specificity, positive predictive value and negative predictive value for GBS PNA Fish for sample processing procedures, with TTR values of 2.5, 2.7 and 9.5 h, were 68, 100, 100 and 95 %; 91, 100, 100 and 98 %; and 100, 100, 100 and 100 %; respectively. Improved test results were achieved by subjecting swabs to an extraction procedure or abbreviated LIM broth enrichment culture incubation prior to performing GBS PNA Fish.

  • Rapid detection of Streptococcus agalactiae from swabs by Peptide nucleic acid fluorescence in situ hybridization.
    Journal of medical microbiology, 2009
    Co-Authors: Heidrun Peltroche-llacsahuanga, Mark J. Fiandaca, Sabine Von Oy, Rudolf Lütticken, Gerhard Haase
    Abstract:

    The applicability of the PNA Fish (Peptide nucleic acid fluorescence in situ hybridization) method for detection of Streptococcus agalactiae [group B streptococci (GBS)] from swab samples was evaluated. Three swab-sample-processing protocols with different time-to-result (TTR) values were compared: (i) direct smearing of fresh swabs onto microscope slides (n=153, TTR 2.5 h), (ii) further extraction and concentration of cells from these same swabs (n=153, TTR 2.7 h), and (iii) short-term LIM broth enrichment culture incubation (7 h, 37 degrees C) of fresh swabs (n=120, TTR 9.5 h). The sensitivity, specificity, positive predictive value and negative predictive value for GBS PNA Fish for sample processing procedures, with TTR values of 2.5, 2.7 and 9.5 h, were 68, 100, 100 and 95 %; 91, 100, 100 and 98 %; and 100, 100, 100 and 100 %; respectively. Improved test results were achieved by subjecting swabs to an extraction procedure or abbreviated LIM broth enrichment culture incubation prior to performing GBS PNA Fish.

Heidrun Peltroche-llacsahuanga - One of the best experts on this subject based on the ideXlab platform.

  • Rapid detection of Streptococcus agalactiae from swabs by Peptide nucleic acid fluorescence in situ hybridization.
    Journal of Medical Microbiology, 2009
    Co-Authors: Heidrun Peltroche-llacsahuanga, Mark J. Fiandaca, Sabine Von Oy, Rudolf Lütticken, Gerhard Haase
    Abstract:

    The applicability of the PNA Fish (Peptide nucleic acid fluorescence in situ hybridization) method for detection of Streptococcus agalactiae [group B streptococci (GBS)] from swab samples was evaluated. Three swab-sample-processing protocols with different time-to-result (TTR) values were compared: (i) direct smearing of fresh swabs onto microscope slides (n=153, TTR 2.5 h), (ii) further extraction and concentration of cells from these same swabs (n=153, TTR 2.7 h), and (iii) short-term LIM broth enrichment culture incubation (7 h, 37 °C) of fresh swabs (n=120, TTR 9.5 h). The sensitivity, specificity, positive predictive value and negative predictive value for GBS PNA Fish for sample processing procedures, with TTR values of 2.5, 2.7 and 9.5 h, were 68, 100, 100 and 95 %; 91, 100, 100 and 98 %; and 100, 100, 100 and 100 %; respectively. Improved test results were achieved by subjecting swabs to an extraction procedure or abbreviated LIM broth enrichment culture incubation prior to performing GBS PNA Fish.

  • Rapid detection of Streptococcus agalactiae from swabs by Peptide nucleic acid fluorescence in situ hybridization.
    Journal of medical microbiology, 2009
    Co-Authors: Heidrun Peltroche-llacsahuanga, Mark J. Fiandaca, Sabine Von Oy, Rudolf Lütticken, Gerhard Haase
    Abstract:

    The applicability of the PNA Fish (Peptide nucleic acid fluorescence in situ hybridization) method for detection of Streptococcus agalactiae [group B streptococci (GBS)] from swab samples was evaluated. Three swab-sample-processing protocols with different time-to-result (TTR) values were compared: (i) direct smearing of fresh swabs onto microscope slides (n=153, TTR 2.5 h), (ii) further extraction and concentration of cells from these same swabs (n=153, TTR 2.7 h), and (iii) short-term LIM broth enrichment culture incubation (7 h, 37 degrees C) of fresh swabs (n=120, TTR 9.5 h). The sensitivity, specificity, positive predictive value and negative predictive value for GBS PNA Fish for sample processing procedures, with TTR values of 2.5, 2.7 and 9.5 h, were 68, 100, 100 and 95 %; 91, 100, 100 and 98 %; and 100, 100, 100 and 100 %; respectively. Improved test results were achieved by subjecting swabs to an extraction procedure or abbreviated LIM broth enrichment culture incubation prior to performing GBS PNA Fish.

Mark J. Fiandaca - One of the best experts on this subject based on the ideXlab platform.

  • Rapid detection of Streptococcus agalactiae from swabs by Peptide nucleic acid fluorescence in situ hybridization.
    Journal of Medical Microbiology, 2009
    Co-Authors: Heidrun Peltroche-llacsahuanga, Mark J. Fiandaca, Sabine Von Oy, Rudolf Lütticken, Gerhard Haase
    Abstract:

    The applicability of the PNA Fish (Peptide nucleic acid fluorescence in situ hybridization) method for detection of Streptococcus agalactiae [group B streptococci (GBS)] from swab samples was evaluated. Three swab-sample-processing protocols with different time-to-result (TTR) values were compared: (i) direct smearing of fresh swabs onto microscope slides (n=153, TTR 2.5 h), (ii) further extraction and concentration of cells from these same swabs (n=153, TTR 2.7 h), and (iii) short-term LIM broth enrichment culture incubation (7 h, 37 °C) of fresh swabs (n=120, TTR 9.5 h). The sensitivity, specificity, positive predictive value and negative predictive value for GBS PNA Fish for sample processing procedures, with TTR values of 2.5, 2.7 and 9.5 h, were 68, 100, 100 and 95 %; 91, 100, 100 and 98 %; and 100, 100, 100 and 100 %; respectively. Improved test results were achieved by subjecting swabs to an extraction procedure or abbreviated LIM broth enrichment culture incubation prior to performing GBS PNA Fish.

  • Rapid detection of Streptococcus agalactiae from swabs by Peptide nucleic acid fluorescence in situ hybridization.
    Journal of medical microbiology, 2009
    Co-Authors: Heidrun Peltroche-llacsahuanga, Mark J. Fiandaca, Sabine Von Oy, Rudolf Lütticken, Gerhard Haase
    Abstract:

    The applicability of the PNA Fish (Peptide nucleic acid fluorescence in situ hybridization) method for detection of Streptococcus agalactiae [group B streptococci (GBS)] from swab samples was evaluated. Three swab-sample-processing protocols with different time-to-result (TTR) values were compared: (i) direct smearing of fresh swabs onto microscope slides (n=153, TTR 2.5 h), (ii) further extraction and concentration of cells from these same swabs (n=153, TTR 2.7 h), and (iii) short-term LIM broth enrichment culture incubation (7 h, 37 degrees C) of fresh swabs (n=120, TTR 9.5 h). The sensitivity, specificity, positive predictive value and negative predictive value for GBS PNA Fish for sample processing procedures, with TTR values of 2.5, 2.7 and 9.5 h, were 68, 100, 100 and 95 %; 91, 100, 100 and 98 %; and 100, 100, 100 and 100 %; respectively. Improved test results were achieved by subjecting swabs to an extraction procedure or abbreviated LIM broth enrichment culture incubation prior to performing GBS PNA Fish.

Sabine Von Oy - One of the best experts on this subject based on the ideXlab platform.

  • Rapid detection of Streptococcus agalactiae from swabs by Peptide nucleic acid fluorescence in situ hybridization.
    Journal of Medical Microbiology, 2009
    Co-Authors: Heidrun Peltroche-llacsahuanga, Mark J. Fiandaca, Sabine Von Oy, Rudolf Lütticken, Gerhard Haase
    Abstract:

    The applicability of the PNA Fish (Peptide nucleic acid fluorescence in situ hybridization) method for detection of Streptococcus agalactiae [group B streptococci (GBS)] from swab samples was evaluated. Three swab-sample-processing protocols with different time-to-result (TTR) values were compared: (i) direct smearing of fresh swabs onto microscope slides (n=153, TTR 2.5 h), (ii) further extraction and concentration of cells from these same swabs (n=153, TTR 2.7 h), and (iii) short-term LIM broth enrichment culture incubation (7 h, 37 °C) of fresh swabs (n=120, TTR 9.5 h). The sensitivity, specificity, positive predictive value and negative predictive value for GBS PNA Fish for sample processing procedures, with TTR values of 2.5, 2.7 and 9.5 h, were 68, 100, 100 and 95 %; 91, 100, 100 and 98 %; and 100, 100, 100 and 100 %; respectively. Improved test results were achieved by subjecting swabs to an extraction procedure or abbreviated LIM broth enrichment culture incubation prior to performing GBS PNA Fish.

  • Rapid detection of Streptococcus agalactiae from swabs by Peptide nucleic acid fluorescence in situ hybridization.
    Journal of medical microbiology, 2009
    Co-Authors: Heidrun Peltroche-llacsahuanga, Mark J. Fiandaca, Sabine Von Oy, Rudolf Lütticken, Gerhard Haase
    Abstract:

    The applicability of the PNA Fish (Peptide nucleic acid fluorescence in situ hybridization) method for detection of Streptococcus agalactiae [group B streptococci (GBS)] from swab samples was evaluated. Three swab-sample-processing protocols with different time-to-result (TTR) values were compared: (i) direct smearing of fresh swabs onto microscope slides (n=153, TTR 2.5 h), (ii) further extraction and concentration of cells from these same swabs (n=153, TTR 2.7 h), and (iii) short-term LIM broth enrichment culture incubation (7 h, 37 degrees C) of fresh swabs (n=120, TTR 9.5 h). The sensitivity, specificity, positive predictive value and negative predictive value for GBS PNA Fish for sample processing procedures, with TTR values of 2.5, 2.7 and 9.5 h, were 68, 100, 100 and 95 %; 91, 100, 100 and 98 %; and 100, 100, 100 and 100 %; respectively. Improved test results were achieved by subjecting swabs to an extraction procedure or abbreviated LIM broth enrichment culture incubation prior to performing GBS PNA Fish.