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Detlev Suckau - One of the best experts on this subject based on the ideXlab platform.
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top down de novo protein sequencing of a 13 6 kda camelid single heavy chain antibody by matrix assisted laser desorption ionization Time of Flight Time of Flight mass spectrometry
Analytical Chemistry, 2010Co-Authors: Anja Resemann, Dirk Wunderlich, Ulrich Rothbauer, Bettina Warscheid, Heinrich Leonhardt, Jens Fuchser, Katja Kuhlmann, Detlev SuckauAbstract:The primary structure of a 13.6 kDa single heavy chain camelid antibody (VHH) was determined by matrix-assisted laser desorption ionization-Time-of-Flight/Time-of-Flight (MALDI-TOF/TOF) top-down sequence analysis. The majority of the sequence was obtained by mass spectrometric de novo sequencing, with the N-terminal 14 amino acid residues being determined using T3-sequencing and database interrogation. The determined sequence was confirmed by liquid chromatography−tandem mass spectrometry (LC−MS/MS) analysis of a tryptic digest, which also provided high-energy collisionally induced dissociation (CID) data permitting the clear assignment of 3 of the 14 isobaric Leu/Ile residues. Five of the 11 Leu/Ile ambiguities could be resolved by homology comparisons with known VHH sequences. The monoisotopic molecular weight of the VHH was determined by ultrahigh-resolution orthogonal electrospray (ESI)-TOF analysis and found to be 13 610.6066 Da, in excellent agreement with the established sequence. To our knowledge,...
Giorgio Montaudo - One of the best experts on this subject based on the ideXlab platform.
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sequence determination in aliphatic poly ester amide s by matrix assisted laser desorption ionization Time of Flight and Time of Flight Time of Flight tandem mass spectrometry
Rapid Communications in Mass Spectrometry, 2005Co-Authors: Paola Rizzarelli, Concetto Puglisi, Giorgio MontaudoAbstract:Poly(ester amide)s from dimethyl sebacate or sebacic acid and 2-aminoethanol or 4-amino-1-butanol were characterized by post-source decay matrix-assisted laser desorption/ionization Time-of-Flight (PSD-MALDI-TOF) and Time-of-Flight/Time-of-Flight tandem mass spectrometry (MALDI-TOF/TOF-MS/MS). Sodiated oligomers were selected as precursor ions for dissociation studies. PSD analysis was performed on dimethyl sebacate, dicarboxylic, carboxylic and amino alcohol, and diamino alcohol terminated oligomers. PSD-MALDI-TOF mass spectra yielded information on the fragmentation mechanisms of the poly(ester amide) chains, showing that the main cleavages proceed through a β-hydrogen transfer rearrangement. MALDI-TOF/TOF-MS/MS provided structural information concerning ester/amide sequences in the polymer chains. As expected, together with the ions appearing in the PSD-MALDI mass spectrum, several new abundant fragment ions in the low-mass range are present in MALDI-TOF/TOF-MS/MS spectra. These new product ions proved to be diagnostic and made it possible to establish the presence of random sequences of ester and amide bonds in the poly(ester amide)s samples. Copyright © 2005 John Wiley & Sons, Ltd.
Robert E Mandrell - One of the best experts on this subject based on the ideXlab platform.
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rapid identification of protein biomarkers of escherichia coli o157 h7 by matrix assisted laser desorption ionization Time of Flight Time of Flight mass spectrometry and top down proteomics
Analytical Chemistry, 2010Co-Authors: Clifton K Fagerquist, Brandon R Garbus, William G Miller, Katherine E Williams, Emma Yee, Anna H Bates, Siobhan Boyle, Leslie A Harden, Michael B Cooley, Robert E MandrellAbstract:Six protein biomarkers from two strains of Escherichia coli O157:H7 and one non-O157:H7, nonpathogenic strain of E. coli have been identified by matrix-assisted laser desorption ionization Time-of-Flight−Time-of-Flight tandem mass spectrometry (MALDI-TOF−TOF-MS/MS) and top-down proteomics. Proteins were extracted from bacterial cell lysates, ionized by MALDI, and analyzed by MS/MS. Protein biomarker ions were identified from their sequence-specific fragment ions by comparison to a database of in silico fragment ions derived from bacterial protein sequences. Web-based software, developed in-house, was used to rapidly compare the mass-to-charge (m/z) of MS/MS fragment ions to the m/z of in silico fragment ions derived from hundreds of bacterial protein sequences. A peak matching algorithm and a p-value algorithm were used to independently score and rank identifications on the basis of the number of MS/MS-in silico matches. The six proteins identified were the acid stress chaperone-like proteins, HdeA and Hd...
Jose A Rodriguezmarroyo - One of the best experts on this subject based on the ideXlab platform.
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the type of mat contact vs photocell affects vertical jump height estimated from Flight Time
Journal of Strength and Conditioning Research, 2013Co-Authors: Juan Garcialopez, J C Morante, Ana Oguetaalday, Jose A RodriguezmarroyoAbstract:The purposes of this study were to analyze the validity and reliability of 2 photocell mats and to probe the possible influence of the type of mat (contact vs. photocell) on vertical jump height estimated from Flight Time. In 2 separate studies, 89 and 92 physical students performed 3 countermovement jumps that were simultaneously registered by a Force Plate (gold standard method), 2 photocell mats (SportJump System Pro and ErgoJump Plus), and a contact mat (SportJump-v1.0). The first study showed that the 2 photocell mats underestimated the vertical jump height (1.3 ± 0.2 cm and 5.9 ± 5.2 cm, respectively), but only SportJump System Pro showed a high correlation with the Force Plate (r = 0.999 and 0.676, respectively) and good intraday reliability (coefficient of variation = 2.98 and 15.94%, intraclass correlation coefficients = 0.95�0.97 and 0.45�0.57, respectively). The second study demonstrated a strong correlation (r = 0.994) between the 2 technologies (contact vs. photocell mats) with differences in vertical jump height of 2.0 ± 0.8 cm (95% confidence interval = 1.9�2.1 cm), which depended on both Flight Time and subjects' body mass. In conclusion, SportJump System Pro was a valid and reliable device. The new devices to measure vertical jump height from Flight Time should be validated. The type of mat (contact vs. photocell) affected approximately 6% the vertical jump height (approximately 2 cm in this study), which should be considered in further studies. The use of validated photocell mats instead of the contact mats was recommended.
Nicolas Bavaresco - One of the best experts on this subject based on the ideXlab platform.
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the concurrent validity and reliability of a low cost high speed camera based method for measuring the Flight Time of vertical jumps
Journal of Strength and Conditioning Research, 2014Co-Authors: Carlos Balsalobrefernandez, Carlos Ma Tejerogonzalez, Juan Del Campovecino, Nicolas BavarescoAbstract:Flight Time is the most accurate and frequently used variable when assessing the height of vertical jumps. The purpose of this study was to analyze the validity and reliability of an alternative method (i.e., the HSC-Kinovea method) for measuring the Flight Time and height of vertical jumping using a low-cost high-speed Casio Exilim FH-25 camera (HSC). To this end, 25 subjects performed a total of 125 vertical jumps on an infrared (IR) platform while simultaneously being recorded with a HSC at 240 fps. Subsequently, 2 observers with no experience in video analysis analyzed the 125 videos independently using the open-license Kinovea 0.8.15 software. The Flight Times obtained were then converted into vertical jump heights, and the intraclass correlation coefficient (ICC), Bland-Altman plot, and Pearson correlation coefficient were calculated for those variables. The results showed a perfect correlation agreement (ICC = 1, p < 0.0001) between both observers' measurements of Flight Time and jump height and a highly reliable agreement (ICC = 0.997, p < 0.0001) between the observers' measurements of Flight Time and jump height using the HSC-Kinovea method and those obtained using the IR system, thus explaining 99.5% (p < 0.0001) of the differences (shared variance) obtained using the IR platform. As a result, besides requiring no previous experience in the use of this technology, the HSC-Kinovea method can be considered to provide similarly valid and reliable measurements of Flight Time and vertical jump height as more expensive equipment (i.e., IR). As such, coaches from many sports could use the HSC-Kinovea method to measure the Flight Time and height of their athlete's vertical jumps.