The Experts below are selected from a list of 20259 Experts worldwide ranked by ideXlab platform
Li An Wu - One of the best experts on this subject based on the ideXlab platform.
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development and characterization of a mouse Floxed bmp2 osteoblast cell line that retains osteoblast genotype and phenotype
Cell and Tissue Research, 2011Co-Authors: Li An Wu, Kevin J Donly, Stephen E. Harris, Junsheng Feng, Lynn Wang, Yan Dong Mu, Andrew Baker, Mary MacdougallAbstract:Bone morphogenetic protein 2 (Bmp2) is essential for osteoblast differentiation and osteogenesis. Generation of Floxed Bmp2 osteoblast cell lines is a valuable tool for studying the effects of Bmp2 on osteoblast differentiation and its signaling pathways during skeletal metabolism. Due to relatively limited sources of primary osteoblasts, we have developed cell lines that serve as good surrogate models for the study of osteoblast cell differentiation and bone mineralization. In this study, we established and characterized immortalized mouse Floxed Bmp2 osteoblast cell lines. Primary mouse Floxed Bmp2 osteoblasts were transfected with pSV3-neo and clonally selected. These transfected cells were verified by PCR and immunohistochemistry. To determine the genotype and phenotype of the immortalized cells, cell morphology, proliferation, differentiation and mineralization were analyzed. Also, expression of osteoblast-related gene markers including Runx2, Osx, ATF4, Dlx3, bone sialoprotein, dentin matrix protein 1, osteonectin, osteocalcin and osteopontin were examined by quantitative RT-PCR and immunohistochemistry. These results showed that immortalized Floxed Bmp2 osteoblasts had a higher proliferation rate but preserved their genotypic and phenotypic characteristics similar to the primary cells. Thus, we, for the first time, describe the development of immortalized mouse Floxed Bmp2 osteoblast cell lines and present a useful model to study osteoblast biology mediated by BMP2 and its downstream signaling transduction pathways.
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immortalization and characterization of mouse Floxed bmp2 4 osteoblasts
Biochemical and Biophysical Research Communications, 2009Co-Authors: Li An Wu, Guohua Yuan, Guobin Yang, Iris Ortizgonzalez, Wuchen Yang, Mary Macdougall, Kevin J DonlyAbstract:Generation of a Floxed Bmp2/4 osteoblast cell line is a valuable tool for studying the modulatory effects of Bmp2 and Bmp4 on osteoblast differentiation as well as relevant molecular events. In this study, primary Floxed Bmp2/4 mouse osteoblasts were cultured and transfected with simian virus 40 large T-antigen. Transfection was verified by polymerase chain reaction (PCR) and immunohistochemistry. To examine the characteristics of the transfected cells, morphology, proliferation and mineralization were analyzed, expression of cell-specific genes including Runx2, ATF4, Dlx3, Osx, dentin matrix protein 1, bone sialoprotein, osteopontin, osteocalcin, osteonectin and collagen type I was detected. These results show that transfected Floxed Bmp2/4 osteoblasts bypassed senescence with a higher proliferation rate, but retain the genotypic and phenotypic characteristics similar to the primary cells. Thus, we for the first time demonstrate the establishment of an immortalized mouse Floxed Bmp2/4 osteoblast cell line.
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Immortalization and characterization of mouse Floxed Bmp2/4 osteoblasts.
Biochemical and Biophysical Research Communications, 2009Co-Authors: Li An Wu, Guohua Yuan, Guobin Yang, Wuchen Yang, Mary Macdougall, Kevin J Donly, Iris Ortiz-gonzalez, Stephen E. Harris, Shuo ChenAbstract:Generation of a Floxed Bmp2/4 osteoblast cell line is a valuable tool for studying the modulatory effects of Bmp2 and Bmp4 on osteoblast differentiation as well as relevant molecular events. In this study, primary Floxed Bmp2/4 mouse osteoblasts were cultured and transfected with simian virus 40 large T-antigen. Transfection was verified by polymerase chain reaction (PCR) and immunohistochemistry. To examine the characteristics of the transfected cells, morphology, proliferation and mineralization were analyzed, expression of cell-specific genes including Runx2, ATF4, Dlx3, Osx, dentin matrix protein 1, bone sialoprotein, osteopontin, osteocalcin, osteonectin and collagen type I was detected. These results show that transfected Floxed Bmp2/4 osteoblasts bypassed senescence with a higher proliferation rate, but retain the genotypic and phenotypic characteristics similar to the primary cells. Thus, we for the first time demonstrate the establishment of an immortalized mouse Floxed Bmp2/4 osteoblast cell line.
Kevin J Donly - One of the best experts on this subject based on the ideXlab platform.
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development and characterization of a mouse Floxed bmp2 osteoblast cell line that retains osteoblast genotype and phenotype
Cell and Tissue Research, 2011Co-Authors: Li An Wu, Kevin J Donly, Stephen E. Harris, Junsheng Feng, Lynn Wang, Yan Dong Mu, Andrew Baker, Mary MacdougallAbstract:Bone morphogenetic protein 2 (Bmp2) is essential for osteoblast differentiation and osteogenesis. Generation of Floxed Bmp2 osteoblast cell lines is a valuable tool for studying the effects of Bmp2 on osteoblast differentiation and its signaling pathways during skeletal metabolism. Due to relatively limited sources of primary osteoblasts, we have developed cell lines that serve as good surrogate models for the study of osteoblast cell differentiation and bone mineralization. In this study, we established and characterized immortalized mouse Floxed Bmp2 osteoblast cell lines. Primary mouse Floxed Bmp2 osteoblasts were transfected with pSV3-neo and clonally selected. These transfected cells were verified by PCR and immunohistochemistry. To determine the genotype and phenotype of the immortalized cells, cell morphology, proliferation, differentiation and mineralization were analyzed. Also, expression of osteoblast-related gene markers including Runx2, Osx, ATF4, Dlx3, bone sialoprotein, dentin matrix protein 1, osteonectin, osteocalcin and osteopontin were examined by quantitative RT-PCR and immunohistochemistry. These results showed that immortalized Floxed Bmp2 osteoblasts had a higher proliferation rate but preserved their genotypic and phenotypic characteristics similar to the primary cells. Thus, we, for the first time, describe the development of immortalized mouse Floxed Bmp2 osteoblast cell lines and present a useful model to study osteoblast biology mediated by BMP2 and its downstream signaling transduction pathways.
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immortalization and characterization of mouse Floxed bmp2 4 osteoblasts
Biochemical and Biophysical Research Communications, 2009Co-Authors: Li An Wu, Guohua Yuan, Guobin Yang, Iris Ortizgonzalez, Wuchen Yang, Mary Macdougall, Kevin J DonlyAbstract:Generation of a Floxed Bmp2/4 osteoblast cell line is a valuable tool for studying the modulatory effects of Bmp2 and Bmp4 on osteoblast differentiation as well as relevant molecular events. In this study, primary Floxed Bmp2/4 mouse osteoblasts were cultured and transfected with simian virus 40 large T-antigen. Transfection was verified by polymerase chain reaction (PCR) and immunohistochemistry. To examine the characteristics of the transfected cells, morphology, proliferation and mineralization were analyzed, expression of cell-specific genes including Runx2, ATF4, Dlx3, Osx, dentin matrix protein 1, bone sialoprotein, osteopontin, osteocalcin, osteonectin and collagen type I was detected. These results show that transfected Floxed Bmp2/4 osteoblasts bypassed senescence with a higher proliferation rate, but retain the genotypic and phenotypic characteristics similar to the primary cells. Thus, we for the first time demonstrate the establishment of an immortalized mouse Floxed Bmp2/4 osteoblast cell line.
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Immortalization and characterization of mouse Floxed Bmp2/4 osteoblasts.
Biochemical and Biophysical Research Communications, 2009Co-Authors: Li An Wu, Guohua Yuan, Guobin Yang, Wuchen Yang, Mary Macdougall, Kevin J Donly, Iris Ortiz-gonzalez, Stephen E. Harris, Shuo ChenAbstract:Generation of a Floxed Bmp2/4 osteoblast cell line is a valuable tool for studying the modulatory effects of Bmp2 and Bmp4 on osteoblast differentiation as well as relevant molecular events. In this study, primary Floxed Bmp2/4 mouse osteoblasts were cultured and transfected with simian virus 40 large T-antigen. Transfection was verified by polymerase chain reaction (PCR) and immunohistochemistry. To examine the characteristics of the transfected cells, morphology, proliferation and mineralization were analyzed, expression of cell-specific genes including Runx2, ATF4, Dlx3, Osx, dentin matrix protein 1, bone sialoprotein, osteopontin, osteocalcin, osteonectin and collagen type I was detected. These results show that transfected Floxed Bmp2/4 osteoblasts bypassed senescence with a higher proliferation rate, but retain the genotypic and phenotypic characteristics similar to the primary cells. Thus, we for the first time demonstrate the establishment of an immortalized mouse Floxed Bmp2/4 osteoblast cell line.
Mary Macdougall - One of the best experts on this subject based on the ideXlab platform.
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development and characterization of a mouse Floxed bmp2 osteoblast cell line that retains osteoblast genotype and phenotype
Cell and Tissue Research, 2011Co-Authors: Li An Wu, Kevin J Donly, Stephen E. Harris, Junsheng Feng, Lynn Wang, Yan Dong Mu, Andrew Baker, Mary MacdougallAbstract:Bone morphogenetic protein 2 (Bmp2) is essential for osteoblast differentiation and osteogenesis. Generation of Floxed Bmp2 osteoblast cell lines is a valuable tool for studying the effects of Bmp2 on osteoblast differentiation and its signaling pathways during skeletal metabolism. Due to relatively limited sources of primary osteoblasts, we have developed cell lines that serve as good surrogate models for the study of osteoblast cell differentiation and bone mineralization. In this study, we established and characterized immortalized mouse Floxed Bmp2 osteoblast cell lines. Primary mouse Floxed Bmp2 osteoblasts were transfected with pSV3-neo and clonally selected. These transfected cells were verified by PCR and immunohistochemistry. To determine the genotype and phenotype of the immortalized cells, cell morphology, proliferation, differentiation and mineralization were analyzed. Also, expression of osteoblast-related gene markers including Runx2, Osx, ATF4, Dlx3, bone sialoprotein, dentin matrix protein 1, osteonectin, osteocalcin and osteopontin were examined by quantitative RT-PCR and immunohistochemistry. These results showed that immortalized Floxed Bmp2 osteoblasts had a higher proliferation rate but preserved their genotypic and phenotypic characteristics similar to the primary cells. Thus, we, for the first time, describe the development of immortalized mouse Floxed Bmp2 osteoblast cell lines and present a useful model to study osteoblast biology mediated by BMP2 and its downstream signaling transduction pathways.
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immortalization and characterization of mouse Floxed bmp2 4 osteoblasts
Biochemical and Biophysical Research Communications, 2009Co-Authors: Li An Wu, Guohua Yuan, Guobin Yang, Iris Ortizgonzalez, Wuchen Yang, Mary Macdougall, Kevin J DonlyAbstract:Generation of a Floxed Bmp2/4 osteoblast cell line is a valuable tool for studying the modulatory effects of Bmp2 and Bmp4 on osteoblast differentiation as well as relevant molecular events. In this study, primary Floxed Bmp2/4 mouse osteoblasts were cultured and transfected with simian virus 40 large T-antigen. Transfection was verified by polymerase chain reaction (PCR) and immunohistochemistry. To examine the characteristics of the transfected cells, morphology, proliferation and mineralization were analyzed, expression of cell-specific genes including Runx2, ATF4, Dlx3, Osx, dentin matrix protein 1, bone sialoprotein, osteopontin, osteocalcin, osteonectin and collagen type I was detected. These results show that transfected Floxed Bmp2/4 osteoblasts bypassed senescence with a higher proliferation rate, but retain the genotypic and phenotypic characteristics similar to the primary cells. Thus, we for the first time demonstrate the establishment of an immortalized mouse Floxed Bmp2/4 osteoblast cell line.
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Immortalization and characterization of mouse Floxed Bmp2/4 osteoblasts.
Biochemical and Biophysical Research Communications, 2009Co-Authors: Li An Wu, Guohua Yuan, Guobin Yang, Wuchen Yang, Mary Macdougall, Kevin J Donly, Iris Ortiz-gonzalez, Stephen E. Harris, Shuo ChenAbstract:Generation of a Floxed Bmp2/4 osteoblast cell line is a valuable tool for studying the modulatory effects of Bmp2 and Bmp4 on osteoblast differentiation as well as relevant molecular events. In this study, primary Floxed Bmp2/4 mouse osteoblasts were cultured and transfected with simian virus 40 large T-antigen. Transfection was verified by polymerase chain reaction (PCR) and immunohistochemistry. To examine the characteristics of the transfected cells, morphology, proliferation and mineralization were analyzed, expression of cell-specific genes including Runx2, ATF4, Dlx3, Osx, dentin matrix protein 1, bone sialoprotein, osteopontin, osteocalcin, osteonectin and collagen type I was detected. These results show that transfected Floxed Bmp2/4 osteoblasts bypassed senescence with a higher proliferation rate, but retain the genotypic and phenotypic characteristics similar to the primary cells. Thus, we for the first time demonstrate the establishment of an immortalized mouse Floxed Bmp2/4 osteoblast cell line.
Guobin Yang - One of the best experts on this subject based on the ideXlab platform.
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immortalization and characterization of mouse Floxed bmp2 4 osteoblasts
Biochemical and Biophysical Research Communications, 2009Co-Authors: Li An Wu, Guohua Yuan, Guobin Yang, Iris Ortizgonzalez, Wuchen Yang, Mary Macdougall, Kevin J DonlyAbstract:Generation of a Floxed Bmp2/4 osteoblast cell line is a valuable tool for studying the modulatory effects of Bmp2 and Bmp4 on osteoblast differentiation as well as relevant molecular events. In this study, primary Floxed Bmp2/4 mouse osteoblasts were cultured and transfected with simian virus 40 large T-antigen. Transfection was verified by polymerase chain reaction (PCR) and immunohistochemistry. To examine the characteristics of the transfected cells, morphology, proliferation and mineralization were analyzed, expression of cell-specific genes including Runx2, ATF4, Dlx3, Osx, dentin matrix protein 1, bone sialoprotein, osteopontin, osteocalcin, osteonectin and collagen type I was detected. These results show that transfected Floxed Bmp2/4 osteoblasts bypassed senescence with a higher proliferation rate, but retain the genotypic and phenotypic characteristics similar to the primary cells. Thus, we for the first time demonstrate the establishment of an immortalized mouse Floxed Bmp2/4 osteoblast cell line.
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Immortalization and characterization of mouse Floxed Bmp2/4 osteoblasts.
Biochemical and Biophysical Research Communications, 2009Co-Authors: Li An Wu, Guohua Yuan, Guobin Yang, Wuchen Yang, Mary Macdougall, Kevin J Donly, Iris Ortiz-gonzalez, Stephen E. Harris, Shuo ChenAbstract:Generation of a Floxed Bmp2/4 osteoblast cell line is a valuable tool for studying the modulatory effects of Bmp2 and Bmp4 on osteoblast differentiation as well as relevant molecular events. In this study, primary Floxed Bmp2/4 mouse osteoblasts were cultured and transfected with simian virus 40 large T-antigen. Transfection was verified by polymerase chain reaction (PCR) and immunohistochemistry. To examine the characteristics of the transfected cells, morphology, proliferation and mineralization were analyzed, expression of cell-specific genes including Runx2, ATF4, Dlx3, Osx, dentin matrix protein 1, bone sialoprotein, osteopontin, osteocalcin, osteonectin and collagen type I was detected. These results show that transfected Floxed Bmp2/4 osteoblasts bypassed senescence with a higher proliferation rate, but retain the genotypic and phenotypic characteristics similar to the primary cells. Thus, we for the first time demonstrate the establishment of an immortalized mouse Floxed Bmp2/4 osteoblast cell line.
Guohua Yuan - One of the best experts on this subject based on the ideXlab platform.
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immortalization and characterization of mouse Floxed bmp2 4 osteoblasts
Biochemical and Biophysical Research Communications, 2009Co-Authors: Li An Wu, Guohua Yuan, Guobin Yang, Iris Ortizgonzalez, Wuchen Yang, Mary Macdougall, Kevin J DonlyAbstract:Generation of a Floxed Bmp2/4 osteoblast cell line is a valuable tool for studying the modulatory effects of Bmp2 and Bmp4 on osteoblast differentiation as well as relevant molecular events. In this study, primary Floxed Bmp2/4 mouse osteoblasts were cultured and transfected with simian virus 40 large T-antigen. Transfection was verified by polymerase chain reaction (PCR) and immunohistochemistry. To examine the characteristics of the transfected cells, morphology, proliferation and mineralization were analyzed, expression of cell-specific genes including Runx2, ATF4, Dlx3, Osx, dentin matrix protein 1, bone sialoprotein, osteopontin, osteocalcin, osteonectin and collagen type I was detected. These results show that transfected Floxed Bmp2/4 osteoblasts bypassed senescence with a higher proliferation rate, but retain the genotypic and phenotypic characteristics similar to the primary cells. Thus, we for the first time demonstrate the establishment of an immortalized mouse Floxed Bmp2/4 osteoblast cell line.
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Immortalization and characterization of mouse Floxed Bmp2/4 osteoblasts.
Biochemical and Biophysical Research Communications, 2009Co-Authors: Li An Wu, Guohua Yuan, Guobin Yang, Wuchen Yang, Mary Macdougall, Kevin J Donly, Iris Ortiz-gonzalez, Stephen E. Harris, Shuo ChenAbstract:Generation of a Floxed Bmp2/4 osteoblast cell line is a valuable tool for studying the modulatory effects of Bmp2 and Bmp4 on osteoblast differentiation as well as relevant molecular events. In this study, primary Floxed Bmp2/4 mouse osteoblasts were cultured and transfected with simian virus 40 large T-antigen. Transfection was verified by polymerase chain reaction (PCR) and immunohistochemistry. To examine the characteristics of the transfected cells, morphology, proliferation and mineralization were analyzed, expression of cell-specific genes including Runx2, ATF4, Dlx3, Osx, dentin matrix protein 1, bone sialoprotein, osteopontin, osteocalcin, osteonectin and collagen type I was detected. These results show that transfected Floxed Bmp2/4 osteoblasts bypassed senescence with a higher proliferation rate, but retain the genotypic and phenotypic characteristics similar to the primary cells. Thus, we for the first time demonstrate the establishment of an immortalized mouse Floxed Bmp2/4 osteoblast cell line.