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Gary W Miller - One of the best experts on this subject based on the ideXlab platform.

  • human suction blister Fluid Composition determined using high resolution metabolomics
    Analytical Chemistry, 2018
    Co-Authors: Megan M Niedzwiecki, Pradnya Samant, Douglas I Walker, Vilinh Tran, Dean P Jones, Mark R Prausnitz, Gary W Miller
    Abstract:

    Interstitial Fluid (ISF) surrounds the cells and tissues of the body. Since ISF has molecular components similar to plasma, as well as compounds produced locally in tissues, it may be a valuable source of biomarkers for diagnostics and monitoring. However, there has not been a comprehensive study to determine the metabolite Composition of ISF and to compare it to plasma. In this study, the metabolome of suction blister Fluid (SBF), which largely consists of ISF, collected from 10 human volunteers was analyzed using untargeted high-resolution metabolomics (HRM). A wide range of metabolites were detected in SBF, including amino acids, lipids, nucleotides, and compounds of exogenous origin. Various systemic and skin-derived metabolite biomarkers were elevated or found uniquely in SBF, and many other metabolites of clinical and physiological significance were well correlated between SBF and plasma. In sum, using untargeted HRM profiling, this study shows that SBF can be a valuable source of information about ...

  • Human Suction Blister Fluid Composition Determined Using High-Resolution Metabolomics
    2018
    Co-Authors: Megan M. Niedzwiecki, Pradnya Samant, Douglas I Walker, Vilinh Tran, Dean P Jones, Mark R Prausnitz, Gary W Miller
    Abstract:

    Interstitial Fluid (ISF) surrounds the cells and tissues of the body. Since ISF has molecular components similar to plasma, as well as compounds produced locally in tissues, it may be a valuable source of biomarkers for diagnostics and monitoring. However, there has not been a comprehensive study to determine the metabolite Composition of ISF and to compare it to plasma. In this study, the metabolome of suction blister Fluid (SBF), which largely consists of ISF, collected from 10 human volunteers was analyzed using untargeted high-resolution metabolomics (HRM). A wide range of metabolites were detected in SBF, including amino acids, lipids, nucleotides, and compounds of exogenous origin. Various systemic and skin-derived metabolite biomarkers were elevated or found uniquely in SBF, and many other metabolites of clinical and physiological significance were well correlated between SBF and plasma. In sum, using untargeted HRM profiling, this study shows that SBF can be a valuable source of information about metabolites relevant to human health

James R Kirkpatrick - One of the best experts on this subject based on the ideXlab platform.

  • role of cations in the methane carbon dioxide partitioning in nano and mesopores of illite using constant reservoir Composition molecular dynamics simulation
    The Journal of Physical Chemistry, 2020
    Co-Authors: Narasimhan Loganathan, Ozgur A Yazaydin, Geoffrey M Bowers, Brice F Ngouanawakou, Andrey G Kalinichev, James R Kirkpatrick
    Abstract:

    We performed constant reservoir Composition molecular dynamics (CRC-MD) simulations at 323 K and 124 bar to quantitatively study the partitioning of Fluid species between the nano- and mesopores of clay and a bulk reservoir containing an equimolar mixture of CO₂ and CH₄. The results show that the basal (001) and protonated edge (010) surfaces of illite both demonstrate a strong preference for CO₂ over CH₄ adsorption; that the (001) surfaces show a stronger preference for CO₂ than the (010) surfaces, especially with K⁺ as the exchangeable cation; and that the structuring of the near-surface CO₂ by K⁺ is stronger than that by Na⁺. The protonated (010) surfaces have a somewhat greater preference for CH₄, with the concentration near them close to that in the bulk Fluid. The effects of the surfaces on the Fluid Composition extend to approximately 2.0 nm from them, with the Fluid Composition at the center of the pore becoming essentially the same as the bulk Composition at a pore thickness of ∼5.7 nm. The preference of nano- and mesopores bounded by clay minerals for CO₂ over CH₄ suggests that injection of CO₂ into tight reservoirs is likely to displace CH₄ into larger pores, thus enhancing its production.

Nigel K H Slater - One of the best experts on this subject based on the ideXlab platform.

  • effect of bore Fluid Composition on microstructure and performance of a microporous hollow fibre membrane as a cation exchange substrate
    Journal of Chromatography A, 2015
    Co-Authors: Radu Alexandru Lazar, Mandal Ipshita, Nigel K H Slater
    Abstract:

    Abstract Micro-capillary film (MCF) membranes are effective platforms for bioseparations and viable alternatives to established packed bed and membrane substrates at the analytical and preparative chromatography scales. Single hollow fibre (HF) MCF membranes with varied microstructures were produced in order to evaluate the effect of the bore Fluid Composition used during hollow fibre extrusion on their structure and performance as cation-exchange adsorbers. Hollow fibres were fabricated from ethylene-vinyl alcohol (EVOH) copolymer through solution extrusion followed by nonsolvent induced phase separation (NIPS) using bore Fluids of differing Composition (100 wt.% N -methyl-2-pyrrolidone (NMP), 100 wt.% glycerol, 100 wt.% water). All HFs displayed highly microporous and mesoporous microstructures, with distinct regions of pore size −1 ) for a flow rate of 1.0 ml min −1 . The NMP–HF–SP module displayed the largest maximum lysozyme binding capacity of all the fibres produced (40.3 mg lysozyme/ml adsorbent volume), a nearly 2-fold increase over the glycerol and 10-fold increase over the water variants at the same sample flow rate. The importance of NMP as a bore Fluid to hollow fibre membrane performance as a result of inner surface porosity was established with a view to applying this parameter for the optimisation of multi-capillary MCF performance in future studies.

Slater, Nigel K. H. - One of the best experts on this subject based on the ideXlab platform.

  • Effect of bore Fluid Composition on microstructure and performance of a microporous hollow fibre membrane as a cation-exchange substrate
    Journal of Chromatography A, 2015
    Co-Authors: Lazar, Radu Alexandru, Mandal Isphita, Slater, Nigel K. H.
    Abstract:

    This is the author accepted manuscript. The final version is available via Elsevier at http://www.sciencedirect.com/science/article/pii/S002196731500432X.Micro-capillary film (MCF) membranes are effective platforms for bioseparations and viable alternatives to established packed bed and membrane substrates at the analytical and preparative chromatography scales. Single hollow fibre (HF) MCF membranes with varied microstructures were produced in order to evaluate the effect of the bore Fluid Composition used during hollow fibre extrusion on their structure and performance as cation-exchange adsorbers. Hollow fibres were fabricated from ethylene-vinyl alcohol (EVOH) copolymer through solution extrusion followed by nonsolvent induced phase separation (NIPS) using bore Fluids of differing Composition (100wt.% N-methyl-2-pyrrolidone (NMP), 100wt.% glycerol, 100wt.% water). All HFs displayed highly microporous and mesoporous microstructures, with distinct regions of pore size

Christopher W Mcintyre - One of the best experts on this subject based on the ideXlab platform.

  • effects of peritoneal dialysis Fluid biocompatibility on baroreflex sensitivity
    Kidney International, 2008
    Co-Authors: S G John, Nicholas M Selby, Christopher W Mcintyre
    Abstract:

    Conventional low biocompatibility peritoneal dialysis (PD) Fluid Composition has been driven by manufacturing expediency and cost limitations. PD is associated with significant acute changes in cardiovascular functional parameters, at least in part influenced by Fluid Composition. Short-term control of blood pressure (BP) is under control of the baroreflex arc. The aim of this study was to investigate the effects of PD Fluid biocompatibility on baroreflex sensitivity (BRS). We studied 10 non-diabetic established continuous ambulatory PD patients, in a randomized crossover trial comparing conventional and biocompatible PD Fluids. Systemic hemodynamics were continuously monitored using digital pulse-wave analysis. Plasma glucose and insulin were assessed during treatment with both 1.36% and 3.86% glucose-containing Fluids. BRS was calculated offline from continuous BP and interbeat interval data. BRS was significantly higher with conventional PD Fluid during both 1.36% ( P P