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Harry E. Boddé - One of the best experts on this subject based on the ideXlab platform.

  • buccal delivery of Fluorescein Isothiocyanate Dextran 4400 and the peptide drug buserelin with glycodeoxycholate as an absorption enhancer in pigs
    Journal of Controlled Release, 1996
    Co-Authors: A J Hoogstraate, A Pijpers, Leo Van Leengoed, J C Verhoef, Jos H. M. Verheijden, Hans E. Junginger, Harry E. Boddé
    Abstract:

    Delivery of drugs via the buccal mucosa is an alternative for the low oral absorption and inconvenient parenteral administration of hydrophilic macromolecular drugs. Due to the low permeability of the buccal epithelium the use of absorption enhancers is a prerequisite. In this study, buccal delivery of Fluorescein Isothiocyanate-labelled Dextran 4400 (FD4) and the peptide drug buserelin was investigated in vivo, in pigs. The delivery device consisted of an application chamber with a solution of FD4 or buserelin, and was attached to the buccal mucosa for 4 h using an adhesive patch. A randomized cross-over study including intravenous administration and buccal delivery without and with 10 mM sodium glycodeoxycholate (GDC) as absorption enhancer was performed in pigs. After buccal administration steady-state plasma levels were rapidly achieved. Co-administration of 10 mM GDC increased the absolute bioavailability from 1.8 ± 0.5% to 12.7 ± 2.0% for FD4, and from 1.0 ± 0.3% to 5.3 ± 1.1% for buserelin. From the present studies it is concluded that buccal administration is a suitable route of delivery for macromolecules and hydrophilic compounds such as peptide drugs.

  • in vivo buccal delivery of Fluorescein Isothiocyanate Dextran 4400 with glycodeoxycholate as an absorption enhancer in pigs
    Journal of Pharmaceutical Sciences, 1996
    Co-Authors: A J Hoogstraate, A Pijpers, Leo Van Leengoed, J C Verhoef, Jos H. M. Verheijden, Hans E. Junginger, Harry E. Boddé
    Abstract:

    Buccal delivery of Fluorescein Isothiocyanate labeled Dextran 4400 (FD4) was investigated in-vivo in pigs. The delivery device consisted of an application chamber with a solution of FD4 and was adhered to the buccal mucosa for 4 h using an adhesive patch. A randomized crossover study including intravenous administration and buccal delivery without and with 10 mM sodium glycodeoxycholate (GDC) as absorption enhancer was performed in five pigs. After buccal administration, steady state plasma levels were rapidly reached. Coadministration of 10 mM GDC increased the absolute bioavailability of FD4 from 1.8 ± 0.5% to 12.7 ± 2.0%. Since FD4 is a macromolecular and hydrophilic compound such as peptide and protein drugs, buccal delivery would provide an adequate alternative to the parenteral administration of these drugs.

Hans E. Junginger - One of the best experts on this subject based on the ideXlab platform.

  • evaluation of superporous hydrogel sph and sph composite in porcine intestine ex vivo assessment of drug transport morphology effect and mechanical fixation to intestinal wall
    European Journal of Pharmaceutics and Biopharmaceutics, 2002
    Co-Authors: Farid Abedin Dorkoosh, Gerrit Borchard, Morteza Rafieetehrani, Coos J Verhoef, Hans E. Junginger
    Abstract:

    Abstract The objective of this study was to investigate the potential of superporous hydrogel (SPH) and SPH composite (SPHC) polymers to enhance the transport of N -α-benzoyl- l -arginine ethylester (BAEE) and Fluorescein Isothiocyanate-Dextran 4400 (FD4) across porcine intestinal epithelium ex-vivo, and to study any possible morphological damage to the epithelium by applying these polymers. In addition, the ability of these polymers to attach to the gut wall by mechanical pressure was examined by using a specifically designed centrifuge model. The transport of BAEE and FD4 across the intestinal mucosa was enhanced 2- to 3-fold by applying SPHC polymer in comparison to negative control. No significant morphological damage was observed by applying these polymers inside the intestinal lumen. Moreover, the SPH and SPHC polymers were able to attach mechanically to the intestinal wall by swelling and did not move in the intestinal lumen even when a horizontal force of 13 gms −2 was applied. In conclusion, these polymers are appropriate vehicles for enhancing the intestinal absorption of peptide and protein drugs.

  • buccal delivery of Fluorescein Isothiocyanate Dextran 4400 and the peptide drug buserelin with glycodeoxycholate as an absorption enhancer in pigs
    Journal of Controlled Release, 1996
    Co-Authors: A J Hoogstraate, A Pijpers, Leo Van Leengoed, J C Verhoef, Jos H. M. Verheijden, Hans E. Junginger, Harry E. Boddé
    Abstract:

    Delivery of drugs via the buccal mucosa is an alternative for the low oral absorption and inconvenient parenteral administration of hydrophilic macromolecular drugs. Due to the low permeability of the buccal epithelium the use of absorption enhancers is a prerequisite. In this study, buccal delivery of Fluorescein Isothiocyanate-labelled Dextran 4400 (FD4) and the peptide drug buserelin was investigated in vivo, in pigs. The delivery device consisted of an application chamber with a solution of FD4 or buserelin, and was attached to the buccal mucosa for 4 h using an adhesive patch. A randomized cross-over study including intravenous administration and buccal delivery without and with 10 mM sodium glycodeoxycholate (GDC) as absorption enhancer was performed in pigs. After buccal administration steady-state plasma levels were rapidly achieved. Co-administration of 10 mM GDC increased the absolute bioavailability from 1.8 ± 0.5% to 12.7 ± 2.0% for FD4, and from 1.0 ± 0.3% to 5.3 ± 1.1% for buserelin. From the present studies it is concluded that buccal administration is a suitable route of delivery for macromolecules and hydrophilic compounds such as peptide drugs.

  • in vivo buccal delivery of Fluorescein Isothiocyanate Dextran 4400 with glycodeoxycholate as an absorption enhancer in pigs
    Journal of Pharmaceutical Sciences, 1996
    Co-Authors: A J Hoogstraate, A Pijpers, Leo Van Leengoed, J C Verhoef, Jos H. M. Verheijden, Hans E. Junginger, Harry E. Boddé
    Abstract:

    Buccal delivery of Fluorescein Isothiocyanate labeled Dextran 4400 (FD4) was investigated in-vivo in pigs. The delivery device consisted of an application chamber with a solution of FD4 and was adhered to the buccal mucosa for 4 h using an adhesive patch. A randomized crossover study including intravenous administration and buccal delivery without and with 10 mM sodium glycodeoxycholate (GDC) as absorption enhancer was performed in five pigs. After buccal administration, steady state plasma levels were rapidly reached. Coadministration of 10 mM GDC increased the absolute bioavailability of FD4 from 1.8 ± 0.5% to 12.7 ± 2.0%. Since FD4 is a macromolecular and hydrophilic compound such as peptide and protein drugs, buccal delivery would provide an adequate alternative to the parenteral administration of these drugs.

Qin Wang - One of the best experts on this subject based on the ideXlab platform.

  • ionic strength and ph responsive permeability of soy glycinin microcapsules
    Langmuir, 2018
    Co-Authors: Nannan Chen, Jinglin Zhang, Lei Mei, Qin Wang
    Abstract:

    Recently, hollow protein microcapsules have been made simply by heating the microphase separated soy glycinin microdomains. However, the properties (e.g., mechanical properties and permeability) that relate to the application of these microcapsules are unknown. In this study, the permeability of the soy glycinin microcapsules was investigated by confocal laser scanning microscopy (CLSM), as influenced by ionic strength and pH using Fluorescein Isothiocyanate-Dextran (FITC-Dextran). The glycinin microcapsules kept the integrity between pH 1 and 11.5, swelled when pH was below 3 or above pH 11, dissociated at pH above 11.5 and deswelled slightly at pH 1. When the pH increased above 11, the permeability of the microcapsule significantly increased. Remarkably, when the pH was below the isoelectric point of glycinin (≈pH 5), FITC-Dextran spontaneously accumulated inside the microcapsule with a significantly higher concentration than that in bulk solution, as evidenced by the strong intensity increase of fluore...

  • Ionic Strength and pH Responsive Permeability of Soy Glycinin Microcapsules
    2018
    Co-Authors: Nannan Chen, Jinglin Zhang, Lei Mei, Qin Wang
    Abstract:

    Recently, hollow protein microcapsules have been made simply by heating the microphase separated soy glycinin microdomains. However, the properties (e.g., mechanical properties and permeability) that relate to the application of these microcapsules are unknown. In this study, the permeability of the soy glycinin microcapsules was investigated by confocal laser scanning microscopy (CLSM), as influenced by ionic strength and pH using Fluorescein Isothiocyanate-Dextran (FITC-Dextran). The glycinin microcapsules kept the integrity between pH 1 and 11.5, swelled when pH was below 3 or above pH 11, dissociated at pH above 11.5 and deswelled slightly at pH 1. When the pH increased above 11, the permeability of the microcapsule significantly increased. Remarkably, when the pH was below the isoelectric point of glycinin (≈pH 5), FITC-Dextran spontaneously accumulated inside the microcapsule with a significantly higher concentration than that in bulk solution, as evidenced by the strong intensity increase of fluorescence. This unique feature significantly increased the loading amount of FITC-Dextran. The permeability of microcapsules was also increased by adding salt but less significant than by adjusting pH. The surface of the microcapsules became coarser when the permeability increased, which was revealed by scanning electron microscopy. These results show that soy glycinin has a great potential to be used as a wall material to fabricate hollow microcapsules that could find applications in biomedicine and food industry

A J Hoogstraate - One of the best experts on this subject based on the ideXlab platform.

  • buccal delivery of Fluorescein Isothiocyanate Dextran 4400 and the peptide drug buserelin with glycodeoxycholate as an absorption enhancer in pigs
    Journal of Controlled Release, 1996
    Co-Authors: A J Hoogstraate, A Pijpers, Leo Van Leengoed, J C Verhoef, Jos H. M. Verheijden, Hans E. Junginger, Harry E. Boddé
    Abstract:

    Delivery of drugs via the buccal mucosa is an alternative for the low oral absorption and inconvenient parenteral administration of hydrophilic macromolecular drugs. Due to the low permeability of the buccal epithelium the use of absorption enhancers is a prerequisite. In this study, buccal delivery of Fluorescein Isothiocyanate-labelled Dextran 4400 (FD4) and the peptide drug buserelin was investigated in vivo, in pigs. The delivery device consisted of an application chamber with a solution of FD4 or buserelin, and was attached to the buccal mucosa for 4 h using an adhesive patch. A randomized cross-over study including intravenous administration and buccal delivery without and with 10 mM sodium glycodeoxycholate (GDC) as absorption enhancer was performed in pigs. After buccal administration steady-state plasma levels were rapidly achieved. Co-administration of 10 mM GDC increased the absolute bioavailability from 1.8 ± 0.5% to 12.7 ± 2.0% for FD4, and from 1.0 ± 0.3% to 5.3 ± 1.1% for buserelin. From the present studies it is concluded that buccal administration is a suitable route of delivery for macromolecules and hydrophilic compounds such as peptide drugs.

  • in vivo buccal delivery of Fluorescein Isothiocyanate Dextran 4400 with glycodeoxycholate as an absorption enhancer in pigs
    Journal of Pharmaceutical Sciences, 1996
    Co-Authors: A J Hoogstraate, A Pijpers, Leo Van Leengoed, J C Verhoef, Jos H. M. Verheijden, Hans E. Junginger, Harry E. Boddé
    Abstract:

    Buccal delivery of Fluorescein Isothiocyanate labeled Dextran 4400 (FD4) was investigated in-vivo in pigs. The delivery device consisted of an application chamber with a solution of FD4 and was adhered to the buccal mucosa for 4 h using an adhesive patch. A randomized crossover study including intravenous administration and buccal delivery without and with 10 mM sodium glycodeoxycholate (GDC) as absorption enhancer was performed in five pigs. After buccal administration, steady state plasma levels were rapidly reached. Coadministration of 10 mM GDC increased the absolute bioavailability of FD4 from 1.8 ± 0.5% to 12.7 ± 2.0%. Since FD4 is a macromolecular and hydrophilic compound such as peptide and protein drugs, buccal delivery would provide an adequate alternative to the parenteral administration of these drugs.

Juan D Latorre - One of the best experts on this subject based on the ideXlab platform.

  • effect of humic acids on intestinal viscosity leaky gut and ammonia excretion in a 24 hr feed restriction model to induce intestinal permeability in broiler chickens
    Animal Science Journal, 2018
    Co-Authors: Jesus A Magueygonzalez, Xochitl Hernandezvelasco, Mikayla F A Baxter, Ruben Merinoguzman, Guillermo Tellez, Matias A Michel, P A Moore, Bruno Soliscruz, Daniel Hernandezpatlan, Juan D Latorre
    Abstract:

    The purpose of this study was to evaluate the effect of humic acids (HA) on intestinal viscosity, leaky gut and ammonia excretion in a 24 hr feed restriction (FR) model to induce intestinal permeability in chickens. One-day-old male Cobb-Vantress broilers were randomly allocated to one of two groups (n = 25 chickens), with or without 0.2% of isolated HA from worm-compost, and placed in brooder batteries. Chicks had ad libitum access to water and feed for 14 days. Intestinal permeability was induced by 24 hr FR starting at 14 days. At 15 days of age, chickens in both groups were given an appropriate dose of Fluorescein Isothiocyanate Dextran (FITC-d) by oral gavage. Intestine and liver samples were also collected to evaluate viscosity and bacterial translocation (BT), respectively. An increase (p < .05) in intestinal viscosity was observed in the experimental group consuming 0.2% of HA and was confirmed in a published in vitro digestion model that simulates the chemical and physical conditions of the crop, proventriculus and intestine of chickens. Furthermore, the treated group also showed a significant reduction in FITC-d, liver BT and ammonia in the manure. These results suggest that HA have a positive impact in intestinal integrity in chickens.

  • optimizing Fluorescein Isothiocyanate Dextran measurement as a biomarker in a 24 h feed restriction model to induce gut permeability in broiler chickens
    Frontiers in Veterinary Science, 2017
    Co-Authors: Mikayla F A Baxter, Xochitl Hernandezvelasco, Juan D Latorre, A D Wolfenden, Ruben Merinoguzman, Brittany D Mahaffey, Yichao Yang, K D Teague, L E Graham, L R Bielke
    Abstract:

    Fluorescein Isothiocyanate Dextran (FITC-d) is a 3-5kda marker used to measure tight junction permeability. We have previously shown that intestinal barrier function can be adversely affected by stress, poorly digested diets, or feed restriction, resulting in increased intestinal inflammation-associated permeability. However, further optimization adjustments of the current FITC-d methodology are possible to enhance precision and efficacy of results in future. The objective of the present study was to optimize our current model to obtain a larger difference between control and treated groups, by optimizing the FITC-d measurement as a biomarker in a 24 h feed restriction (FR) model to induce gut permeability in broiler chickens. One in vitro and four in vivo independent experiments were conducted. The results of the present study suggest that by increasing the dose of FITC-d, (8.32 mg/kg versus 4.16 mg/kg); shortening the collection time of blood samples, (1 h versus 2.5 h); using a pool of non-FITC-d serum as a blank, compared to previously used PBS; adding a standard curve to set a limit of detection and modifying the software’s optimal sensitivity value it was possible to obtain more consistent and reliable results.

  • Optimizing Fluorescein Isothiocyanate Dextran Measurement As a Biomarker in a 24-h Feed Restriction Model to Induce Gut Permeability in Broiler Chickens
    Frontiers Media S.A., 2017
    Co-Authors: Guillermo Tellez, Juan D Latorre, Mikayla F A Baxter, Brittany D Mahaffey, Yichao Yang, K D Teague, L E Graham, Ruben Merino-guzman, Amanda D. Wolfenden, Xochitl Hernandez-velasco
    Abstract:

    Fluorescein Isothiocyanate Dextran (FITC-d) is a 3–5 kDa marker used to measure tight junction permeability. We have previously shown that intestinal barrier function can be adversely affected by stress, poorly digested diets, or feed restriction (FR), resulting in increased intestinal inflammation-associated permeability. However, further optimization adjustments of the current FITC-d methodology are possible to enhance precision and efficacy of results in future. The objective of the present study was to optimize our current model to obtain a larger difference between control and treated groups, by optimizing the FITC-d measurement as a biomarker in a 24-h FR model to induce gut permeability in broiler chickens. One in vitro and four in vivo independent experiments were conducted. The results of the present study suggest that by increasing the dose of FITC-d (8.32 versus 4.16 mg/kg); shortening the collection time of blood samples (1 versus 2.5 h); using a pool of non-FITC-d serum as a blank, compared to previously used PBS; adding a standard curve to set a limit of detection and modifying the software’s optimal sensitivity value, it was possible to obtain more consistent and reliable results

  • dose titration of fitc d for optimal measurement of enteric inflammation in broiler chicks
    Poultry Science, 2015
    Co-Authors: E A Vicuna, V A Kuttappan, G Tellez, Xochitl Hernandezvelasco, R Seebergalarza, Juan D Latorre, Olivia B Faulkner, A D Wolfenden, B M Hargis, L R Bielke
    Abstract:

    Abstract Traditionally, antibiotic growth promoters (AGP) have been used in foodstock animals to reduce enteric inflammation and maintain intestinal homeostasis, thus improving growth and performance. Due to increasing restrictions regarding the use of AGP however, precise and high throughput enteric inflammation models and markers to search for effective alternatives are urgently needed. In this paper, oral administration of Fluorescein Isothiocyanate Dextran (FITC-d, 3–5 kDa) and its passage into blood was used as a marker for tight junction permeability. In experiement 1, broilers were assigned to a control group, a group which received 24 h feed restriction (FR), or a group which received Dextran sodium sulfate (DSS) (0.75% in water for 5 d), and each group then underwent an oral gavage of FITC-d 2.5 h before sample collection on d10. FITC-d in serum and intestinal samples (duodenum and ceca) were found to be higher (P