The Experts below are selected from a list of 153 Experts worldwide ranked by ideXlab platform
Ulrich J Krull - One of the best experts on this subject based on the ideXlab platform.
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Fluorescence resonance energy transfer (FRET) for DNA biosensors: FRET pairs and Förster distances for various dye-DNA conjugates.
Analytica Chimica Acta, 2006Co-Authors: Melissa Massey, W. Russ Algar, Ulrich J KrullAbstract:Abstract Fluorescence resonance energy transfer (FRET) between the extrinsic dye labels Cyanine 3 (Cy3), Cyanine 5 (Cy5), Carboxytetramethyl Rhodamine (TAMRA), Iowa Black Fluorescence Quencher (IabFQ), and Iowa Black RQ (IabRQ) has been studied. The Forster distances for these FRET-pairs in single- and double-stranded DNA conjugates have been determined. In particular, it should be noted that the quantum yield of the donors Cy3 and TAMRA varies between single- and double-stranded DNA. While this alters the Forster distance for a donor–acceptor pair, this also allows for detection of thermal denaturation events with a single non-intercalating fluorophore. The utility of FRET in the development of nucleic acid biosensor technology is illustrated by using TAMRA and IabRQ as a FRET pair in selectivity experiments. The differential quenching of TAMRA Fluorescence by IabRQ in solution has been used to discriminate between 0 and 3 base pair mismatches at 60 °C for a 19 base sequence. At room temperature, the quenching of TAMRA Fluorescence was not an effective indicator of the degree of base pair mismatch. There appears to be a threshold of duplex stability at room temperature which occurs beyond two base pair mismatches and reverses the observed trend in TAMRA Fluorescence prior to that degree of mismatch. When this experimental system is transferred to a glass surface through covalent coupling and organosilane chemistry, the observed trend in TAMRA Fluorescence at room temperature is similar to that obtained in bulk solution, but without a threshold of duplex stability. In addition to quenching of Fluorescence by FRET, it is believed that several other quenching mechanisms are occurring at the surface.
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Fluorescence resonance energy transfer (FRET) for DNA biosensors: FRET pairs and Förster distances for various dye-DNA conjugates.
Analytica chimica acta, 2006Co-Authors: Melissa Massey, W. Russ Algar, Ulrich J KrullAbstract:Fluorescence resonance energy transfer (FRET) between the extrinsic dye labels Cyanine 3 (Cy3), Cyanine 5 (Cy5), Carboxytetramethyl Rhodamine (TAMRA), Iowa Black Fluorescence Quencher (IabFQ), and Iowa Black RQ (IabRQ) has been studied. The Förster distances for these FRET-pairs in single- and double-stranded DNA conjugates have been determined. In particular, it should be noted that the quantum yield of the donors Cy3 and TAMRA varies between single- and double-stranded DNA. While this alters the Förster distance for a donor-acceptor pair, this also allows for detection of thermal denaturation events with a single non-intercalating fluorophore. The utility of FRET in the development of nucleic acid biosensor technology is illustrated by using TAMRA and IabRQ as a FRET pair in selectivity experiments. The differential quenching of TAMRA Fluorescence by IabRQ in solution has been used to discriminate between 0 and 3 base pair mismatches at 60 degrees C for a 19 base sequence. At room temperature, the quenching of TAMRA Fluorescence was not an effective indicator of the degree of base pair mismatch. There appears to be a threshold of duplex stability at room temperature which occurs beyond two base pair mismatches and reverses the observed trend in TAMRA Fluorescence prior to that degree of mismatch. When this experimental system is transferred to a glass surface through covalent coupling and organosilane chemistry, the observed trend in TAMRA Fluorescence at room temperature is similar to that obtained in bulk solution, but without a threshold of duplex stability. In addition to quenching of Fluorescence by FRET, it is believed that several other quenching mechanisms are occurring at the surface.
Sheryl A Tucker - One of the best experts on this subject based on the ideXlab platform.
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micellar liquid chromatography of polycyclic aromatic hydrocarbons alkylpyridinium chloride as mobile phase modifier and selective Fluorescence Quencher
IEEE Journal of Solid-state Circuits, 2003Co-Authors: Chunfeng Mao, Kelly E Mcgill, Sheryl A TuckerAbstract:The dual role of alkylpyridinium chlorides, cetylpyridinium chloride (CPC) and dodecylpyridinium chloride (DDPC), as micellar mobile phase modifiers and selective Fluorescence quenching agents of polycyclic aromatic hydrocarbons (PAHs), in micellar liquid chromatographic separation of PAHs is reported. The replacement of 0.037 M cetyltrimethylammonium chloride in the aqueous mobile phase with CPC/DDPC Quencher greatly simplifies the observed Fluorescence-detected chromatograms, facilitating PAH identification. The resulting chromatograms are similar to those obtained from the conventional approach - pyridinium chloride Quencher in an acetonitrile mobile phase. To quantify the quenching, the (F 0 /F- 1) values from the Stern-Volmer equation are calculated from the chromatograms and compared. The feasibility of using CPC or DDPC as a dual reagent under isocratic and gradient conditions is shown.
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high performance liquid chromatographic separation of polycyclic aromatic hydrocarbons using pyridinium chloride as a selective Fluorescence Quencher to aid detection
Journal of Chromatography A, 2002Co-Authors: Chunfeng Mao, Sheryl A TuckerAbstract:The first use of pyridinium chloride (PC), as a selective Fluorescence quenching agent of alternant polycyclic aromatic hydrocarbons (PAHs), under HPLC separation conditions is reported. PC was found to be superior to nitromethane, the only reported PAH selective Quencher used in HPLC. The mobile phase addition of 0.03 M PC greatly simplifies the observed Fluorescence-detected chromatograms for complex PAH mixtures, facilitating PAH identification. Stern–Volmer quenching constants (Ksv) for PAHs were calculated from the chromatograms obtained under isocratic and gradient conditions and found to be similar. The Ksv values were shown to be useful in establishing peak purity.
Melissa Massey - One of the best experts on this subject based on the ideXlab platform.
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Fluorescence resonance energy transfer (FRET) for DNA biosensors: FRET pairs and Förster distances for various dye-DNA conjugates.
Analytica Chimica Acta, 2006Co-Authors: Melissa Massey, W. Russ Algar, Ulrich J KrullAbstract:Abstract Fluorescence resonance energy transfer (FRET) between the extrinsic dye labels Cyanine 3 (Cy3), Cyanine 5 (Cy5), Carboxytetramethyl Rhodamine (TAMRA), Iowa Black Fluorescence Quencher (IabFQ), and Iowa Black RQ (IabRQ) has been studied. The Forster distances for these FRET-pairs in single- and double-stranded DNA conjugates have been determined. In particular, it should be noted that the quantum yield of the donors Cy3 and TAMRA varies between single- and double-stranded DNA. While this alters the Forster distance for a donor–acceptor pair, this also allows for detection of thermal denaturation events with a single non-intercalating fluorophore. The utility of FRET in the development of nucleic acid biosensor technology is illustrated by using TAMRA and IabRQ as a FRET pair in selectivity experiments. The differential quenching of TAMRA Fluorescence by IabRQ in solution has been used to discriminate between 0 and 3 base pair mismatches at 60 °C for a 19 base sequence. At room temperature, the quenching of TAMRA Fluorescence was not an effective indicator of the degree of base pair mismatch. There appears to be a threshold of duplex stability at room temperature which occurs beyond two base pair mismatches and reverses the observed trend in TAMRA Fluorescence prior to that degree of mismatch. When this experimental system is transferred to a glass surface through covalent coupling and organosilane chemistry, the observed trend in TAMRA Fluorescence at room temperature is similar to that obtained in bulk solution, but without a threshold of duplex stability. In addition to quenching of Fluorescence by FRET, it is believed that several other quenching mechanisms are occurring at the surface.
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Fluorescence resonance energy transfer (FRET) for DNA biosensors: FRET pairs and Förster distances for various dye-DNA conjugates.
Analytica chimica acta, 2006Co-Authors: Melissa Massey, W. Russ Algar, Ulrich J KrullAbstract:Fluorescence resonance energy transfer (FRET) between the extrinsic dye labels Cyanine 3 (Cy3), Cyanine 5 (Cy5), Carboxytetramethyl Rhodamine (TAMRA), Iowa Black Fluorescence Quencher (IabFQ), and Iowa Black RQ (IabRQ) has been studied. The Förster distances for these FRET-pairs in single- and double-stranded DNA conjugates have been determined. In particular, it should be noted that the quantum yield of the donors Cy3 and TAMRA varies between single- and double-stranded DNA. While this alters the Förster distance for a donor-acceptor pair, this also allows for detection of thermal denaturation events with a single non-intercalating fluorophore. The utility of FRET in the development of nucleic acid biosensor technology is illustrated by using TAMRA and IabRQ as a FRET pair in selectivity experiments. The differential quenching of TAMRA Fluorescence by IabRQ in solution has been used to discriminate between 0 and 3 base pair mismatches at 60 degrees C for a 19 base sequence. At room temperature, the quenching of TAMRA Fluorescence was not an effective indicator of the degree of base pair mismatch. There appears to be a threshold of duplex stability at room temperature which occurs beyond two base pair mismatches and reverses the observed trend in TAMRA Fluorescence prior to that degree of mismatch. When this experimental system is transferred to a glass surface through covalent coupling and organosilane chemistry, the observed trend in TAMRA Fluorescence at room temperature is similar to that obtained in bulk solution, but without a threshold of duplex stability. In addition to quenching of Fluorescence by FRET, it is believed that several other quenching mechanisms are occurring at the surface.
Chunfeng Mao - One of the best experts on this subject based on the ideXlab platform.
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micellar liquid chromatography of polycyclic aromatic hydrocarbons alkylpyridinium chloride as mobile phase modifier and selective Fluorescence Quencher
IEEE Journal of Solid-state Circuits, 2003Co-Authors: Chunfeng Mao, Kelly E Mcgill, Sheryl A TuckerAbstract:The dual role of alkylpyridinium chlorides, cetylpyridinium chloride (CPC) and dodecylpyridinium chloride (DDPC), as micellar mobile phase modifiers and selective Fluorescence quenching agents of polycyclic aromatic hydrocarbons (PAHs), in micellar liquid chromatographic separation of PAHs is reported. The replacement of 0.037 M cetyltrimethylammonium chloride in the aqueous mobile phase with CPC/DDPC Quencher greatly simplifies the observed Fluorescence-detected chromatograms, facilitating PAH identification. The resulting chromatograms are similar to those obtained from the conventional approach - pyridinium chloride Quencher in an acetonitrile mobile phase. To quantify the quenching, the (F 0 /F- 1) values from the Stern-Volmer equation are calculated from the chromatograms and compared. The feasibility of using CPC or DDPC as a dual reagent under isocratic and gradient conditions is shown.
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high performance liquid chromatographic separation of polycyclic aromatic hydrocarbons using pyridinium chloride as a selective Fluorescence Quencher to aid detection
Journal of Chromatography A, 2002Co-Authors: Chunfeng Mao, Sheryl A TuckerAbstract:The first use of pyridinium chloride (PC), as a selective Fluorescence quenching agent of alternant polycyclic aromatic hydrocarbons (PAHs), under HPLC separation conditions is reported. PC was found to be superior to nitromethane, the only reported PAH selective Quencher used in HPLC. The mobile phase addition of 0.03 M PC greatly simplifies the observed Fluorescence-detected chromatograms for complex PAH mixtures, facilitating PAH identification. Stern–Volmer quenching constants (Ksv) for PAHs were calculated from the chromatograms obtained under isocratic and gradient conditions and found to be similar. The Ksv values were shown to be useful in establishing peak purity.
W. Russ Algar - One of the best experts on this subject based on the ideXlab platform.
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Fluorescence resonance energy transfer (FRET) for DNA biosensors: FRET pairs and Förster distances for various dye-DNA conjugates.
Analytica Chimica Acta, 2006Co-Authors: Melissa Massey, W. Russ Algar, Ulrich J KrullAbstract:Abstract Fluorescence resonance energy transfer (FRET) between the extrinsic dye labels Cyanine 3 (Cy3), Cyanine 5 (Cy5), Carboxytetramethyl Rhodamine (TAMRA), Iowa Black Fluorescence Quencher (IabFQ), and Iowa Black RQ (IabRQ) has been studied. The Forster distances for these FRET-pairs in single- and double-stranded DNA conjugates have been determined. In particular, it should be noted that the quantum yield of the donors Cy3 and TAMRA varies between single- and double-stranded DNA. While this alters the Forster distance for a donor–acceptor pair, this also allows for detection of thermal denaturation events with a single non-intercalating fluorophore. The utility of FRET in the development of nucleic acid biosensor technology is illustrated by using TAMRA and IabRQ as a FRET pair in selectivity experiments. The differential quenching of TAMRA Fluorescence by IabRQ in solution has been used to discriminate between 0 and 3 base pair mismatches at 60 °C for a 19 base sequence. At room temperature, the quenching of TAMRA Fluorescence was not an effective indicator of the degree of base pair mismatch. There appears to be a threshold of duplex stability at room temperature which occurs beyond two base pair mismatches and reverses the observed trend in TAMRA Fluorescence prior to that degree of mismatch. When this experimental system is transferred to a glass surface through covalent coupling and organosilane chemistry, the observed trend in TAMRA Fluorescence at room temperature is similar to that obtained in bulk solution, but without a threshold of duplex stability. In addition to quenching of Fluorescence by FRET, it is believed that several other quenching mechanisms are occurring at the surface.
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Fluorescence resonance energy transfer (FRET) for DNA biosensors: FRET pairs and Förster distances for various dye-DNA conjugates.
Analytica chimica acta, 2006Co-Authors: Melissa Massey, W. Russ Algar, Ulrich J KrullAbstract:Fluorescence resonance energy transfer (FRET) between the extrinsic dye labels Cyanine 3 (Cy3), Cyanine 5 (Cy5), Carboxytetramethyl Rhodamine (TAMRA), Iowa Black Fluorescence Quencher (IabFQ), and Iowa Black RQ (IabRQ) has been studied. The Förster distances for these FRET-pairs in single- and double-stranded DNA conjugates have been determined. In particular, it should be noted that the quantum yield of the donors Cy3 and TAMRA varies between single- and double-stranded DNA. While this alters the Förster distance for a donor-acceptor pair, this also allows for detection of thermal denaturation events with a single non-intercalating fluorophore. The utility of FRET in the development of nucleic acid biosensor technology is illustrated by using TAMRA and IabRQ as a FRET pair in selectivity experiments. The differential quenching of TAMRA Fluorescence by IabRQ in solution has been used to discriminate between 0 and 3 base pair mismatches at 60 degrees C for a 19 base sequence. At room temperature, the quenching of TAMRA Fluorescence was not an effective indicator of the degree of base pair mismatch. There appears to be a threshold of duplex stability at room temperature which occurs beyond two base pair mismatches and reverses the observed trend in TAMRA Fluorescence prior to that degree of mismatch. When this experimental system is transferred to a glass surface through covalent coupling and organosilane chemistry, the observed trend in TAMRA Fluorescence at room temperature is similar to that obtained in bulk solution, but without a threshold of duplex stability. In addition to quenching of Fluorescence by FRET, it is believed that several other quenching mechanisms are occurring at the surface.