The Experts below are selected from a list of 57 Experts worldwide ranked by ideXlab platform
Kim D. Thompson - One of the best experts on this subject based on the ideXlab platform.
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Rapid detection and identification of Streptococcus iniae using a monoclonal Antibody-based indirect Fluorescent Antibody Technique
Aquaculture, 2006Co-Authors: Phillip H. Klesius, Joyce J. Evans, Craig A. Shoemaker, Hung-yueh Yeh, Andrew E. Goodwin, Alexandra Adams, Kim D. ThompsonAbstract:Streptococcus iniae is among the major pathogens of a large number of fish species cultured in fresh and marine recirculating and net pen production systems. The traditional plate culture Technique to detect and identify S. iniae is time consuming and may be problematic due to phenotypic variations of S. iniae isolates. The rapid transmission and severe economic impact of this disease necessitates the development of a rapid, reliable, specific, and sensitive Technique to detect and identify S. iniae. We developed an indirect Fluorescent Antibody Technique (IFAT) based on a highly specific mononclonal Antibody for S. iniae. The IFAT was found to be suitable for the detection and identification of S. iniae from experimentally and naturally infected tilapia (Oreochromis niloticus). The olfactory epithelium of the naris of experimentally and naturally infected tilapia was demonstrated to be a reliable, sensitive, and non-lethal sample site for the detection and identification of S. iniae. This IFAT was validated by the traditional plate culture Techniques but found to be considerably less time consuming and problematic. These findings reveal that this IFAT is an improvement in the ability to detect and identify S. iniae in infected and carrier fish.
Siri Sawasdikosol - One of the best experts on this subject based on the ideXlab platform.
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Comparative Study of Fluorescent Antibody Technique for the Diagnosis of Rabies
2014Co-Authors: Siri SawasdikosolAbstract:Specimens sent for rabies diagnosis was simultaneously detected by two methods-the Fluorescent Antibody Technique and mouse inoculation. The total numbers of specimen were 148 of which 90 specimens were found to be positive by Fluorescent Antibody Technique whereas 88 specimens were detected to be positive by mouse inoculation. By calculation Fluorescent Antibody Technique showed 100 percent of sensitivity and percentage of specificity was 96.6
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rabies diagnosis by Fluorescent Antibody Technique and quality control
Bulletin of the Department of Medical Sciences - วารสารกรมวิทยาศาสตร์การแพทย์, 2013Co-Authors: Siri SawasdikosolAbstract:Laboratory diagnosis for Rabies infection is very important. The Technique should be sensitive, specific and reported as soon as possible with reliability. Quality control of the laboratory Technique is really needed in order to assure the diagnosis. Four hundred and seventy dog’s brains were sent to diagnostic laboratories in Bangkok Metropolis and rural areas. The specimens were shipped by mailing, carrying by persons without cold chain and carrying by persons with cold chain systems respectively. Among 370 brain specimens with cold chain system, diagnostic tests were 85% correct. The cold chain system yielded the best outcome. The erratic diagnosis problems in some laboratories were solved by ad hoc supervision of Virus Research Institute, Department of Medical Sciences as a reference laboratory. Compared the diagnostic results of Virus Research Institute, Thai Red Cross and Siriraj Hospital were similar results. Key words: Quality control, Rabies, Cold Chain System.
Phillip H. Klesius - One of the best experts on this subject based on the ideXlab platform.
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Rapid detection and identification of Streptococcus iniae using a monoclonal Antibody-based indirect Fluorescent Antibody Technique
Aquaculture, 2006Co-Authors: Phillip H. Klesius, Joyce J. Evans, Craig A. Shoemaker, Hung-yueh Yeh, Andrew E. Goodwin, Alexandra Adams, Kim D. ThompsonAbstract:Streptococcus iniae is among the major pathogens of a large number of fish species cultured in fresh and marine recirculating and net pen production systems. The traditional plate culture Technique to detect and identify S. iniae is time consuming and may be problematic due to phenotypic variations of S. iniae isolates. The rapid transmission and severe economic impact of this disease necessitates the development of a rapid, reliable, specific, and sensitive Technique to detect and identify S. iniae. We developed an indirect Fluorescent Antibody Technique (IFAT) based on a highly specific mononclonal Antibody for S. iniae. The IFAT was found to be suitable for the detection and identification of S. iniae from experimentally and naturally infected tilapia (Oreochromis niloticus). The olfactory epithelium of the naris of experimentally and naturally infected tilapia was demonstrated to be a reliable, sensitive, and non-lethal sample site for the detection and identification of S. iniae. This IFAT was validated by the traditional plate culture Techniques but found to be considerably less time consuming and problematic. These findings reveal that this IFAT is an improvement in the ability to detect and identify S. iniae in infected and carrier fish.
Andrew E. Goodwin - One of the best experts on this subject based on the ideXlab platform.
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Rapid detection and identification of Streptococcus iniae using a monoclonal Antibody-based indirect Fluorescent Antibody Technique
Aquaculture, 2006Co-Authors: Phillip H. Klesius, Joyce J. Evans, Craig A. Shoemaker, Hung-yueh Yeh, Andrew E. Goodwin, Alexandra Adams, Kim D. ThompsonAbstract:Streptococcus iniae is among the major pathogens of a large number of fish species cultured in fresh and marine recirculating and net pen production systems. The traditional plate culture Technique to detect and identify S. iniae is time consuming and may be problematic due to phenotypic variations of S. iniae isolates. The rapid transmission and severe economic impact of this disease necessitates the development of a rapid, reliable, specific, and sensitive Technique to detect and identify S. iniae. We developed an indirect Fluorescent Antibody Technique (IFAT) based on a highly specific mononclonal Antibody for S. iniae. The IFAT was found to be suitable for the detection and identification of S. iniae from experimentally and naturally infected tilapia (Oreochromis niloticus). The olfactory epithelium of the naris of experimentally and naturally infected tilapia was demonstrated to be a reliable, sensitive, and non-lethal sample site for the detection and identification of S. iniae. This IFAT was validated by the traditional plate culture Techniques but found to be considerably less time consuming and problematic. These findings reveal that this IFAT is an improvement in the ability to detect and identify S. iniae in infected and carrier fish.
Joyce J. Evans - One of the best experts on this subject based on the ideXlab platform.
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Rapid detection and identification of Streptococcus iniae using a monoclonal Antibody-based indirect Fluorescent Antibody Technique
Aquaculture, 2006Co-Authors: Phillip H. Klesius, Joyce J. Evans, Craig A. Shoemaker, Hung-yueh Yeh, Andrew E. Goodwin, Alexandra Adams, Kim D. ThompsonAbstract:Streptococcus iniae is among the major pathogens of a large number of fish species cultured in fresh and marine recirculating and net pen production systems. The traditional plate culture Technique to detect and identify S. iniae is time consuming and may be problematic due to phenotypic variations of S. iniae isolates. The rapid transmission and severe economic impact of this disease necessitates the development of a rapid, reliable, specific, and sensitive Technique to detect and identify S. iniae. We developed an indirect Fluorescent Antibody Technique (IFAT) based on a highly specific mononclonal Antibody for S. iniae. The IFAT was found to be suitable for the detection and identification of S. iniae from experimentally and naturally infected tilapia (Oreochromis niloticus). The olfactory epithelium of the naris of experimentally and naturally infected tilapia was demonstrated to be a reliable, sensitive, and non-lethal sample site for the detection and identification of S. iniae. This IFAT was validated by the traditional plate culture Techniques but found to be considerably less time consuming and problematic. These findings reveal that this IFAT is an improvement in the ability to detect and identify S. iniae in infected and carrier fish.