The Experts below are selected from a list of 216 Experts worldwide ranked by ideXlab platform

Tapio Mäkelä - One of the best experts on this subject based on the ideXlab platform.

  • development of a novel hapten for radioimmunoassay of the lignan enterolactone in plasma serum total synthesis of trans 5 carboxymethoxyenterolactone and several analogues
    Tetrahedron, 2000
    Co-Authors: Tapio Mäkelä, Jorma Matikainen, Kristiina Wähälä, Tapio Hase
    Abstract:

    Abstract A recently developed method for the analysis of the mammalian lignan, enterolactone 1, is based on time-resolved Fluoroimmunoassay (TR-FIA) using an europium chelate as a label. This RIA utilizes enterolactone derivatives carrying a carboxylic acid appendage for the production of antiserum and tracer. The synthesis of 5-carboxymethoxyenterolactone 6 and analogues 5, 7 and 8 is described, and their suitability for the method are discussed.

  • Development of a Novel Hapten for Radioimmunoassay of the Lignan, Enterolactone in Plasma (Serum). Total Synthesis of (±)-trans-5-Carboxymethoxyenterolactone and Several Analogues
    Tetrahedron, 2000
    Co-Authors: Tapio Mäkelä, Jorma Matikainen, Kristiina Wähälä, Tapio Hase
    Abstract:

    Abstract A recently developed method for the analysis of the mammalian lignan, enterolactone 1, is based on time-resolved Fluoroimmunoassay (TR-FIA) using an europium chelate as a label. This RIA utilizes enterolactone derivatives carrying a carboxylic acid appendage for the production of antiserum and tracer. The synthesis of 5-carboxymethoxyenterolactone 6 and analogues 5, 7 and 8 is described, and their suitability for the method are discussed.

  • time resolved Fluoroimmunoassay for plasma enterolactone
    Analytical Biochemistry, 1998
    Co-Authors: Herman Adlercreutz, Guojie J Wang, Katariina Lusa, Marjo Talme, Tapio Mäkelä, O Lapcik, Richard Hampl, Kristiina Wähälä, Heikki Mikola
    Abstract:

    Abstract We present a method for the determination of the lignan enterolactone in plasma (serum). This compound, produced by intestinal bacteria from matairesinol and secoisolariciresinol in fiber-rich food, is a biomarker related to the intake of a healthy diet. The method is based on time-resolved Fluoroimmunoassay using a europium chelate as a label. After synthesis of 5′-O-carboxymethoxyenterolactone the compound is coupled to bovine serum albumin and then used as antigen in immunization of rabbits. The tracer with the europium chelate is synthesized using the same 5′-derivative of enterolactone. After enzymatic hydrolysis and ether extraction the immunoassay is carried out using the VICTOR 1420 multilabel counter (Wallac Oy, Turku, Finland). No antiserum cross-reactivity with available lignans, isoflavonoids, or flavonoids could be detected. The intraassay and interassay coefficients of variation at different concentrations vary 4.6–6.0 and 5.5–9.9, respectively. The working range of the assay is 1.5–540 nmol/liter. We measured enterolactone in serum/plasma of 224 Finnish subjects: 98.8% of the subjects had values

H. Michael Dosch - One of the best experts on this subject based on the ideXlab platform.

  • Disease-associated anti-bovine serum albumin antibodies in type 1 (insulin-dependent) diabetes mellitus are detected by particle concentration Fluoroimmunoassay, and not by enzyme linked immunoassay.
    Diabetologia, 1992
    Co-Authors: Jesse Karjalainen, Erkki Savilahti, Tapio Saukkonen, H. Michael Dosch
    Abstract:

    We recently developed a particle concentration Fluoroimmunoassay for the measurement of serum antibodies to bovine serum albumin in patients with Type 1 (insulin-dependent) diabetes mellitus. We observed elevated IgG-anti-bovine serum albumin antibodies in 100% of newly-diagnosed diabetic children and in 2.5% of matched control children. Here we compare the Fluoroimmunoassay and the more commonly available enzyme linked immunoassay technique, exchanging coded serum samples from 40 newly-diagnosed diabetic children and 179 control children between two laboratories. Particle concentration Fluoroimmunoassay detected elevated IgG-anti-bovine serum albumin antibodies in all diabetic children, enzyme immunoassay in 25% (p

  • Disease-associated anti-bovine serum albumin antibodies in Type 1 (insulin-dependent) diabetes mellitus are detected by particle concentration Fluoroimmunoassay, and not by enzyme linked immunoassay
    Diabetologia, 1992
    Co-Authors: Jesse Karjalainen, Erkki Savilahti, Tapio Saukkonen, H. Michael Dosch
    Abstract:

    We recently developed a particle concentration Fluoroimmunoassay for the measurement of serum antibodies to bovine serum albumin in patients with Type 1 (insulin-dependent) diabetes mellitus. We observed elevated IgG-anti-bovine serum albumin antibodies in 100% of newly-diagnosed diabetic children and in 2.5% of matched control children. Here we compare the Fluoroimmunoassay and the more commonly available enzyme linked immunoassay technique, exchanging coded serum samples from 40 newly-diagnosed diabetic children and 179 control children between two laboratories. Particle concentration Fluoroimmunoassay detected elevated IgG-anti-bovine serum albumin antibodies in all diabetic children, enzyme immunoassay in 25% (p less than 0.0001). Fluoroimmunoassay detected elevated levels in 2.2% and enzyme immunoassay in 10% of control children (p less than 0.002). Elevated IgA-anti-bovine serum albumin antibodies in patients were slightly more often detected by Fluoroimmunoassay than by enzyme immunoassay, while in control children enzyme immunoassays detected elevated levels three times more often (p less than 0.01). Values measured in either assay showed overall no correlation in either patient (IgG:rs = 0.28; IgA:rs = 0.11) or control sera (IgG:rs = 0.02; IgA:rs = -0.05). Fluoroimmunoassay for IgG was 100% disease-sensitive (enzyme immunoassay: 25%, p less than 0.0001) and more disease-specific (IgG; p less than 0.02). Our findings demonstrate that these assay techniques detected distinct subsets of anti-bovine serum albumin antibodies with little (IgG) or some (IgA) overlap. In Fluoroimmunoassay procedures, antigen:antibody binding occurs within 1-2 min while hours are allowed in an enzyme immunoassay.

Kristiina Wähälä - One of the best experts on this subject based on the ideXlab platform.

  • development of a novel hapten for radioimmunoassay of the lignan enterolactone in plasma serum total synthesis of trans 5 carboxymethoxyenterolactone and several analogues
    Tetrahedron, 2000
    Co-Authors: Tapio Mäkelä, Jorma Matikainen, Kristiina Wähälä, Tapio Hase
    Abstract:

    Abstract A recently developed method for the analysis of the mammalian lignan, enterolactone 1, is based on time-resolved Fluoroimmunoassay (TR-FIA) using an europium chelate as a label. This RIA utilizes enterolactone derivatives carrying a carboxylic acid appendage for the production of antiserum and tracer. The synthesis of 5-carboxymethoxyenterolactone 6 and analogues 5, 7 and 8 is described, and their suitability for the method are discussed.

  • Development of a Novel Hapten for Radioimmunoassay of the Lignan, Enterolactone in Plasma (Serum). Total Synthesis of (±)-trans-5-Carboxymethoxyenterolactone and Several Analogues
    Tetrahedron, 2000
    Co-Authors: Tapio Mäkelä, Jorma Matikainen, Kristiina Wähälä, Tapio Hase
    Abstract:

    Abstract A recently developed method for the analysis of the mammalian lignan, enterolactone 1, is based on time-resolved Fluoroimmunoassay (TR-FIA) using an europium chelate as a label. This RIA utilizes enterolactone derivatives carrying a carboxylic acid appendage for the production of antiserum and tracer. The synthesis of 5-carboxymethoxyenterolactone 6 and analogues 5, 7 and 8 is described, and their suitability for the method are discussed.

  • time resolved Fluoroimmunoassay for plasma enterolactone
    Analytical Biochemistry, 1998
    Co-Authors: Herman Adlercreutz, Guojie J Wang, Katariina Lusa, Marjo Talme, Tapio Mäkelä, O Lapcik, Richard Hampl, Kristiina Wähälä, Heikki Mikola
    Abstract:

    Abstract We present a method for the determination of the lignan enterolactone in plasma (serum). This compound, produced by intestinal bacteria from matairesinol and secoisolariciresinol in fiber-rich food, is a biomarker related to the intake of a healthy diet. The method is based on time-resolved Fluoroimmunoassay using a europium chelate as a label. After synthesis of 5′-O-carboxymethoxyenterolactone the compound is coupled to bovine serum albumin and then used as antigen in immunization of rabbits. The tracer with the europium chelate is synthesized using the same 5′-derivative of enterolactone. After enzymatic hydrolysis and ether extraction the immunoassay is carried out using the VICTOR 1420 multilabel counter (Wallac Oy, Turku, Finland). No antiserum cross-reactivity with available lignans, isoflavonoids, or flavonoids could be detected. The intraassay and interassay coefficients of variation at different concentrations vary 4.6–6.0 and 5.5–9.9, respectively. The working range of the assay is 1.5–540 nmol/liter. We measured enterolactone in serum/plasma of 224 Finnish subjects: 98.8% of the subjects had values

  • time resolved Fluoroimmunoassay for plasma enterolactone
    Analytical Biochemistry, 1998
    Co-Authors: Herman Adlercreutz, Guojie J Wang, Katariina Lusa, Marjo Talme, O Lapcik, Richard Hampl, Kristiina Wähälä, Taru Makela, Heikki Mikola
    Abstract:

    We present a method for the determination of the lignan enterolactone in plasma (serum). This compound, produced by intestinal bacteria from matairesinol and secoisolariciresinol in fiber-rich food, is a biomarker related to the intake of a healthy diet. The method is based on time-resolved Fluoroimmunoassay using a europium chelate as a label. After synthesis of 5′-O-carboxymethoxyenterolactone the compound is coupled to bovine serum albumin and then used as antigen in immunization of rabbits. The tracer with the europium chelate is synthesized using the same 5′-derivative of enterolactone. After enzymatic hydrolysis and ether extraction the immunoassay is carried out using the VICTOR 1420 multilabel counter (Wallac Oy, Turku, Finland). No antiserum cross-reactivity with available lignans, isoflavonoids, or flavonoids could be detected. The intraassay and interassay coefficients of variation at different concentrations vary 4.6–6.0 and 5.5–9.9, respectively. The working range of the assay is 1.5–540 nmol/liter. We measured enterolactone in serum/plasma of 224 Finnish subjects: 98.8% of the subjects had values <100 nmol/liter, 38.0% had 20–39.9 nmol/liter, and 34.4% had <20 nmol/liter.

Tapio Hase - One of the best experts on this subject based on the ideXlab platform.

Jesse Karjalainen - One of the best experts on this subject based on the ideXlab platform.

  • Disease-associated anti-bovine serum albumin antibodies in type 1 (insulin-dependent) diabetes mellitus are detected by particle concentration Fluoroimmunoassay, and not by enzyme linked immunoassay.
    Diabetologia, 1992
    Co-Authors: Jesse Karjalainen, Erkki Savilahti, Tapio Saukkonen, H. Michael Dosch
    Abstract:

    We recently developed a particle concentration Fluoroimmunoassay for the measurement of serum antibodies to bovine serum albumin in patients with Type 1 (insulin-dependent) diabetes mellitus. We observed elevated IgG-anti-bovine serum albumin antibodies in 100% of newly-diagnosed diabetic children and in 2.5% of matched control children. Here we compare the Fluoroimmunoassay and the more commonly available enzyme linked immunoassay technique, exchanging coded serum samples from 40 newly-diagnosed diabetic children and 179 control children between two laboratories. Particle concentration Fluoroimmunoassay detected elevated IgG-anti-bovine serum albumin antibodies in all diabetic children, enzyme immunoassay in 25% (p

  • Disease-associated anti-bovine serum albumin antibodies in Type 1 (insulin-dependent) diabetes mellitus are detected by particle concentration Fluoroimmunoassay, and not by enzyme linked immunoassay
    Diabetologia, 1992
    Co-Authors: Jesse Karjalainen, Erkki Savilahti, Tapio Saukkonen, H. Michael Dosch
    Abstract:

    We recently developed a particle concentration Fluoroimmunoassay for the measurement of serum antibodies to bovine serum albumin in patients with Type 1 (insulin-dependent) diabetes mellitus. We observed elevated IgG-anti-bovine serum albumin antibodies in 100% of newly-diagnosed diabetic children and in 2.5% of matched control children. Here we compare the Fluoroimmunoassay and the more commonly available enzyme linked immunoassay technique, exchanging coded serum samples from 40 newly-diagnosed diabetic children and 179 control children between two laboratories. Particle concentration Fluoroimmunoassay detected elevated IgG-anti-bovine serum albumin antibodies in all diabetic children, enzyme immunoassay in 25% (p less than 0.0001). Fluoroimmunoassay detected elevated levels in 2.2% and enzyme immunoassay in 10% of control children (p less than 0.002). Elevated IgA-anti-bovine serum albumin antibodies in patients were slightly more often detected by Fluoroimmunoassay than by enzyme immunoassay, while in control children enzyme immunoassays detected elevated levels three times more often (p less than 0.01). Values measured in either assay showed overall no correlation in either patient (IgG:rs = 0.28; IgA:rs = 0.11) or control sera (IgG:rs = 0.02; IgA:rs = -0.05). Fluoroimmunoassay for IgG was 100% disease-sensitive (enzyme immunoassay: 25%, p less than 0.0001) and more disease-specific (IgG; p less than 0.02). Our findings demonstrate that these assay techniques detected distinct subsets of anti-bovine serum albumin antibodies with little (IgG) or some (IgA) overlap. In Fluoroimmunoassay procedures, antigen:antibody binding occurs within 1-2 min while hours are allowed in an enzyme immunoassay.