The Experts below are selected from a list of 1344 Experts worldwide ranked by ideXlab platform
Egidio Barbi - One of the best experts on this subject based on the ideXlab platform.
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normal saline flushes performed once daily maintain peripheral intravenous catheter patency a randomised controlled trial
2015Co-Authors: Silvana Schreiber, Chiara Zanchi, Luca Ronfani, Anna Delise, Alessandra Corbelli, Rosamaria Bortoluzzi, Andrea Taddio, Egidio BarbiAbstract:Objective Recent evidence supports the use of normal saline flushes in place of heparin to maintain the patency of peripheral intravenous locks (IVLs); however, there are no data regarding the recommended flush frequency. Study design This was an open, non-inferiority, randomised controlled trial. Children with IVLs, aged 1–17 years, were randomly assigned to receive saline Flushing every 12 h (group A) or every 24 h (group B). The main outcome was the maintenance of catheter patency. Results Four hundred patients were randomised; 198 subjects were analysed in the 12 h group and 199 in the 24 h group (three patients were lost at follow-up). Occlusion occurred in 15 children (7.6%) in group A versus 9 (4.5%) in group B (p=0.21). The difference in catheter patency was +3.1% in favour of the 24 h group (95% CI −1.6% to 7.7%), showing the non-inferiority of the 24 h Procedure (the non-inferiority margin was set at −4%). Catheter-related complications were not different between the two groups (12.1% in group A vs 9.5% in group B; p=0.42). Conclusions A Flushing Procedure with one flush per day allows maintenance of catheter patency without an increase in catheter-related complications. We propose a simplification of the Flushing Procedure with only one flush per day, thereby reducing costs (materials use and nursing time), labour and unnecessary manipulation of the catheters which can cause distress in younger children and their parents. Trial registration number The study is registered in the international database ClinicalTrial.gov under registration number NCT02221024.
Andy M Scutt - One of the best experts on this subject based on the ideXlab platform.
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centrifugal isolation of bone marrow from bone an improved method for the recovery and quantitation of bone marrow osteoprogenitor cells from rat tibiae and femurae
1999Co-Authors: K Dobson, L Reading, M Haberey, X Marine, Andy M ScuttAbstract:The high variation often observed in the ex vivo fibroblastic-colony forming unit (CFU-f) assay is likely to be due to both biological and experimental variation. To determine whether we could improve experimental methods we developed an alternative method of bone marrow cell (BMC) isolation employing a centrifugation step. The osteogenic capacity of centrifugally isolated BMC was compared to that of BMC that were isolated using the standard ``Flushing'' technique using the CFU-f assay. The centrifugation method was found to be both quick and simple to perform and allowed simultaneous preparation of all samples. Centrifugually isolated BMC gave rise to approximately 100% more cfu-ap and cfu-f in cultures from both tibiae and femurae. The proportion of alkaline phosphatase positive colonies remained the same and colony morphologies were similar for both isolation methods. Histological comparison of the flushed and spun bones showed that after the Flushing Procedure many cells remained in the marrow cavity especially in the trabecular area. In contrast, centrifugation completely emptied the marrow space of all cells except bone lining cells and osteoblasts. Thus the osteogenic capacity of the bone marrow can be expressed as the number of CFU-f per bone instead of the frequency as is the norm. Using these methods to isolate BMC for ex vivo investigations should lead to a reduction in CFU-f number variation due to the isolation method.
Silvana Schreiber - One of the best experts on this subject based on the ideXlab platform.
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normal saline flushes performed once daily maintain peripheral intravenous catheter patency a randomised controlled trial
2015Co-Authors: Silvana Schreiber, Chiara Zanchi, Luca Ronfani, Anna Delise, Alessandra Corbelli, Rosamaria Bortoluzzi, Andrea Taddio, Egidio BarbiAbstract:Objective Recent evidence supports the use of normal saline flushes in place of heparin to maintain the patency of peripheral intravenous locks (IVLs); however, there are no data regarding the recommended flush frequency. Study design This was an open, non-inferiority, randomised controlled trial. Children with IVLs, aged 1–17 years, were randomly assigned to receive saline Flushing every 12 h (group A) or every 24 h (group B). The main outcome was the maintenance of catheter patency. Results Four hundred patients were randomised; 198 subjects were analysed in the 12 h group and 199 in the 24 h group (three patients were lost at follow-up). Occlusion occurred in 15 children (7.6%) in group A versus 9 (4.5%) in group B (p=0.21). The difference in catheter patency was +3.1% in favour of the 24 h group (95% CI −1.6% to 7.7%), showing the non-inferiority of the 24 h Procedure (the non-inferiority margin was set at −4%). Catheter-related complications were not different between the two groups (12.1% in group A vs 9.5% in group B; p=0.42). Conclusions A Flushing Procedure with one flush per day allows maintenance of catheter patency without an increase in catheter-related complications. We propose a simplification of the Flushing Procedure with only one flush per day, thereby reducing costs (materials use and nursing time), labour and unnecessary manipulation of the catheters which can cause distress in younger children and their parents. Trial registration number The study is registered in the international database ClinicalTrial.gov under registration number NCT02221024.
Chiara Zanchi - One of the best experts on this subject based on the ideXlab platform.
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normal saline flushes performed once daily maintain peripheral intravenous catheter patency a randomised controlled trial
2015Co-Authors: Silvana Schreiber, Chiara Zanchi, Luca Ronfani, Anna Delise, Alessandra Corbelli, Rosamaria Bortoluzzi, Andrea Taddio, Egidio BarbiAbstract:Objective Recent evidence supports the use of normal saline flushes in place of heparin to maintain the patency of peripheral intravenous locks (IVLs); however, there are no data regarding the recommended flush frequency. Study design This was an open, non-inferiority, randomised controlled trial. Children with IVLs, aged 1–17 years, were randomly assigned to receive saline Flushing every 12 h (group A) or every 24 h (group B). The main outcome was the maintenance of catheter patency. Results Four hundred patients were randomised; 198 subjects were analysed in the 12 h group and 199 in the 24 h group (three patients were lost at follow-up). Occlusion occurred in 15 children (7.6%) in group A versus 9 (4.5%) in group B (p=0.21). The difference in catheter patency was +3.1% in favour of the 24 h group (95% CI −1.6% to 7.7%), showing the non-inferiority of the 24 h Procedure (the non-inferiority margin was set at −4%). Catheter-related complications were not different between the two groups (12.1% in group A vs 9.5% in group B; p=0.42). Conclusions A Flushing Procedure with one flush per day allows maintenance of catheter patency without an increase in catheter-related complications. We propose a simplification of the Flushing Procedure with only one flush per day, thereby reducing costs (materials use and nursing time), labour and unnecessary manipulation of the catheters which can cause distress in younger children and their parents. Trial registration number The study is registered in the international database ClinicalTrial.gov under registration number NCT02221024.
Luca Ronfani - One of the best experts on this subject based on the ideXlab platform.
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normal saline flushes performed once daily maintain peripheral intravenous catheter patency a randomised controlled trial
2015Co-Authors: Silvana Schreiber, Chiara Zanchi, Luca Ronfani, Anna Delise, Alessandra Corbelli, Rosamaria Bortoluzzi, Andrea Taddio, Egidio BarbiAbstract:Objective Recent evidence supports the use of normal saline flushes in place of heparin to maintain the patency of peripheral intravenous locks (IVLs); however, there are no data regarding the recommended flush frequency. Study design This was an open, non-inferiority, randomised controlled trial. Children with IVLs, aged 1–17 years, were randomly assigned to receive saline Flushing every 12 h (group A) or every 24 h (group B). The main outcome was the maintenance of catheter patency. Results Four hundred patients were randomised; 198 subjects were analysed in the 12 h group and 199 in the 24 h group (three patients were lost at follow-up). Occlusion occurred in 15 children (7.6%) in group A versus 9 (4.5%) in group B (p=0.21). The difference in catheter patency was +3.1% in favour of the 24 h group (95% CI −1.6% to 7.7%), showing the non-inferiority of the 24 h Procedure (the non-inferiority margin was set at −4%). Catheter-related complications were not different between the two groups (12.1% in group A vs 9.5% in group B; p=0.42). Conclusions A Flushing Procedure with one flush per day allows maintenance of catheter patency without an increase in catheter-related complications. We propose a simplification of the Flushing Procedure with only one flush per day, thereby reducing costs (materials use and nursing time), labour and unnecessary manipulation of the catheters which can cause distress in younger children and their parents. Trial registration number The study is registered in the international database ClinicalTrial.gov under registration number NCT02221024.