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S Chastantmaillard - One of the best experts on this subject based on the ideXlab platform.
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in vitro embryo production efficiency in cattle and its association with oocyte adenosine triphosphate content quantity of mitochondrial dna and mitochondrial dna haplogroup
Biology of Reproduction, 2004Co-Authors: M Tamassia, F Nuttinck, Pascale Maypanloup, Pascal Reynier, Y Heyman, Gilles Charpigny, Miodrag Stojkovic, S Hiendleder, J P Renard, S ChastantmaillardAbstract:Abstract Mitochondria have a broad range of functions that affect reproduction, and structural as well as quantitative variation in mtDNA has been associated with gamete quality and reproductive success. To investigate the mitochondria effect on in vitro embryo production, we collected oocytes by ultrasound-guided Follicular Aspiration from donor cows known to differ in the developmental capacity, measured by the blastocyst formation rate, of their oocytes. To evaluate the potential effects of mtDNA and mitochondrial function on oocyte quality, the donor cows' mtDNA control region was sequenced and, after pairwise comparisons of polymorphisms, animals were grouped into two major haplogroups. The number of mtDNA molecules per oocyte was quantified by real-time PCR, and the adenosine triphosphate (ATP) content was measured in each oocyte to identify variations between haplogroups. Overall, ATP stocks in oocytes of the two haplogroups differed significantly (P < 0.05; means ± SEM) both at the germinal vesicl...
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in vitro embryo production efficiency in cattle and its association with oocyte adenosine triphosphate content quantity of mitochondrial dna and mitochondrial dna haplogroup
Biology of Reproduction, 2004Co-Authors: M Tamassia, F Nuttinck, Pascale Maypanloup, Pascal Reynier, Y Heyman, Gilles Charpigny, Miodrag Stojkovic, S Hiendleder, J P Renard, S ChastantmaillardAbstract:Mitochondria have a broad range of functions that affect reproduction, and structural as well as quantitative variation in mtDNA has been associated with gamete quality and reproductive success. To investigate the mitochondria effect on in vitro embryo production, we collected oocytes by ultrasound-guided Follicular Aspiration from donor cows known to differ in the developmental capacity, measured by the blastocyst formation rate, of their oocytes. To evaluate the potential effects of mtDNA and mitochondrial function on oocyte quality, the donor cows' mtDNA control region was sequenced and, after pairwise comparisons of polymorphisms, animals were grouped into two major haplogroups. The number of mtDNA molecules per oocyte was quantified by real-time PCR, and the adenosine triphosphate (ATP) content was measured in each oocyte to identify variations between haplogroups. Overall, ATP stocks in oocytes of the two haplogroups differed significantly (P < 0.05; means +/- SEM) both at the germinal vesicle and metaphase II stages (2.8 +/- 0.06 pmol vs. 2.6 +/- 0.07 pmol and 2.9 +/- 0.1 pmol vs. 2.3 +/- 0.06 pmol, respectively). The proportion of development to blastocyst was significantly different between haplogroups (22.3 +/- 2.1 % vs. 36.7 +/- 2.9 %). The number of mtDNA molecules per oocyte was highly variable (377 327 +/- 14 104, ranging from 2.0 x 10(3) to 1.2 x 10(6)) but not significantly different between the two haplogroups; significant differences were observed between animals without any apparent relationship to blastocyst production. These data suggest that mitochondria and mtDNA haplogroup affect the developmental capacity of bovine oocytes in vitro.
Neri Laufer - One of the best experts on this subject based on the ideXlab platform.
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ovarian hyperstimulation syndrome in novel reproductive technologies prevention and treatment
Fertility and Sterility, 1992Co-Authors: Daniel Navot, Neri Laufer, P A BerghAbstract:Objective To overview the world literature on ovarian hyperstimulation syndrome (OHSS) and modes of prevention and treatment of OHSS. Study Selection All the pertinent literature on OHSS, its prevention, and strategies for treatment were reviewed. Prevention Key to prevention is proper identification of the population at risk, which includes women with either the hormonal or the morphological signs of polycystic ovarian disease, high serum estradiol (E 2 ) before human chorionic gonadotropin (hCG) administration (E 2 > 4,000 pg/mL), multiple Follicular response (>35), younger age, and lean habitus. When a high risk situation is recognized, ovulatory dose of hCG may be reduced, avoided (with cycle cancellation), or substituted by gonadotropin-releasing hormone or its agonist. Luteal support with hCG is to be bypassed. To minimize risk of OHSS, endogenous pregnancy-drived hCG may be eluded by judicious cryopreservation of all embryos. Last, Follicular Aspiration will allow higher levels of E 2 and larger number of follicles to be matured with lesser risk of OHSS than conventional ovulation induction without Follicular Aspiration. Treatment In-house for the severe and intensive care for the critical form. Meticulous fluid and electrolyte balance using both crystalloids and colloids (albumin) until hemoconcentration abates. Paracentesis is indicated for tight ascites, deteriorating kidney functions, and symptomatic relief. Diuretics may be prudently used once hemodilution is achieved. Dopamine drip may be used as a renal rescue, whereas heparin is indicated for thromboembolic phenomena and surgery reserved for abdominal catastrophies. Therapeutic interruption of an early gestation may be lifesaving when all other measures have failed. Conclusions Although severe and critical OHSS may not be completely avoided, early recognition of high-risk factors, judicious prevention schemes, and treatment strategies should reduce the complications and long-term sequelae of this iatrogenic syndrome.
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the correlation between interleukin 2 and soluble interleukin 2 receptors to oestradiol progesterone and testosterone levels in periovulatory follicles of in vitro fertilization patients
Human Reproduction, 1992Co-Authors: Vivian Barak, Nathan Mordel, Gershom Zajicek, Ina Kalichman, Abraham J Treves, Neri LauferAbstract:The present study was performed to evaluate the correlation between Follicular fluid levels of interleukin 2 (IL-2) and IL-2 soluble receptor (sIL-2R), oestradiol, progesterone and testosterone levels, oocyte fertilization, embryo quality and pregnancy rates. Twenty-eight patients with a pure tubal factor and undergoing in-vitro fertilization and embryo transfer were randomly chosen and treated with gonadotrophin releasing hormone agonist (GnRHa) in the midluteal phase (long protocol) coupled with Follicular phase administration of human menopausal gonadotrophin. Transvaginal Follicular Aspiration was performed 36 h after human chorionic gonadotrophin administration, followed 48 h later by embryo transfer. One hundred and twenty-three Follicular fluids were sampled. The mean Follicular fluid levels (+/- SD) were 2.30 +/- 0.80 fmol for IL-2, 458.2 +/- 236.0 units/ml for sIL-2R, 28.5 +/- 58.1 ng/ml for oestradiol, 2360.5 +/- 2846 ng/ml for progesterone and 7.22 +/- 7.08 ng/ml for testosterone. There was a significant (P less than 0.01) correlation between IL-2 and testosterone levels. No correlation was found between the lymphokines and serum oestradiol, Follicular fluid progesterone, oocyte fertilization, embryo quality and pregnancy. It may be concluded that significant concentrations of IL-2 and sIL-2R exist in Follicular fluid. Wide variations in Follicular IL-2 and sIL-2R concentrations of different follicles were found in the same patients.
M Tamassia - One of the best experts on this subject based on the ideXlab platform.
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in vitro embryo production efficiency in cattle and its association with oocyte adenosine triphosphate content quantity of mitochondrial dna and mitochondrial dna haplogroup
Biology of Reproduction, 2004Co-Authors: M Tamassia, F Nuttinck, Pascale Maypanloup, Pascal Reynier, Y Heyman, Gilles Charpigny, Miodrag Stojkovic, S Hiendleder, J P Renard, S ChastantmaillardAbstract:Abstract Mitochondria have a broad range of functions that affect reproduction, and structural as well as quantitative variation in mtDNA has been associated with gamete quality and reproductive success. To investigate the mitochondria effect on in vitro embryo production, we collected oocytes by ultrasound-guided Follicular Aspiration from donor cows known to differ in the developmental capacity, measured by the blastocyst formation rate, of their oocytes. To evaluate the potential effects of mtDNA and mitochondrial function on oocyte quality, the donor cows' mtDNA control region was sequenced and, after pairwise comparisons of polymorphisms, animals were grouped into two major haplogroups. The number of mtDNA molecules per oocyte was quantified by real-time PCR, and the adenosine triphosphate (ATP) content was measured in each oocyte to identify variations between haplogroups. Overall, ATP stocks in oocytes of the two haplogroups differed significantly (P < 0.05; means ± SEM) both at the germinal vesicl...
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in vitro embryo production efficiency in cattle and its association with oocyte adenosine triphosphate content quantity of mitochondrial dna and mitochondrial dna haplogroup
Biology of Reproduction, 2004Co-Authors: M Tamassia, F Nuttinck, Pascale Maypanloup, Pascal Reynier, Y Heyman, Gilles Charpigny, Miodrag Stojkovic, S Hiendleder, J P Renard, S ChastantmaillardAbstract:Mitochondria have a broad range of functions that affect reproduction, and structural as well as quantitative variation in mtDNA has been associated with gamete quality and reproductive success. To investigate the mitochondria effect on in vitro embryo production, we collected oocytes by ultrasound-guided Follicular Aspiration from donor cows known to differ in the developmental capacity, measured by the blastocyst formation rate, of their oocytes. To evaluate the potential effects of mtDNA and mitochondrial function on oocyte quality, the donor cows' mtDNA control region was sequenced and, after pairwise comparisons of polymorphisms, animals were grouped into two major haplogroups. The number of mtDNA molecules per oocyte was quantified by real-time PCR, and the adenosine triphosphate (ATP) content was measured in each oocyte to identify variations between haplogroups. Overall, ATP stocks in oocytes of the two haplogroups differed significantly (P < 0.05; means +/- SEM) both at the germinal vesicle and metaphase II stages (2.8 +/- 0.06 pmol vs. 2.6 +/- 0.07 pmol and 2.9 +/- 0.1 pmol vs. 2.3 +/- 0.06 pmol, respectively). The proportion of development to blastocyst was significantly different between haplogroups (22.3 +/- 2.1 % vs. 36.7 +/- 2.9 %). The number of mtDNA molecules per oocyte was highly variable (377 327 +/- 14 104, ranging from 2.0 x 10(3) to 1.2 x 10(6)) but not significantly different between the two haplogroups; significant differences were observed between animals without any apparent relationship to blastocyst production. These data suggest that mitochondria and mtDNA haplogroup affect the developmental capacity of bovine oocytes in vitro.
S Hiendleder - One of the best experts on this subject based on the ideXlab platform.
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in vitro embryo production efficiency in cattle and its association with oocyte adenosine triphosphate content quantity of mitochondrial dna and mitochondrial dna haplogroup
Biology of Reproduction, 2004Co-Authors: M Tamassia, F Nuttinck, Pascale Maypanloup, Pascal Reynier, Y Heyman, Gilles Charpigny, Miodrag Stojkovic, S Hiendleder, J P Renard, S ChastantmaillardAbstract:Abstract Mitochondria have a broad range of functions that affect reproduction, and structural as well as quantitative variation in mtDNA has been associated with gamete quality and reproductive success. To investigate the mitochondria effect on in vitro embryo production, we collected oocytes by ultrasound-guided Follicular Aspiration from donor cows known to differ in the developmental capacity, measured by the blastocyst formation rate, of their oocytes. To evaluate the potential effects of mtDNA and mitochondrial function on oocyte quality, the donor cows' mtDNA control region was sequenced and, after pairwise comparisons of polymorphisms, animals were grouped into two major haplogroups. The number of mtDNA molecules per oocyte was quantified by real-time PCR, and the adenosine triphosphate (ATP) content was measured in each oocyte to identify variations between haplogroups. Overall, ATP stocks in oocytes of the two haplogroups differed significantly (P < 0.05; means ± SEM) both at the germinal vesicl...
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in vitro embryo production efficiency in cattle and its association with oocyte adenosine triphosphate content quantity of mitochondrial dna and mitochondrial dna haplogroup
Biology of Reproduction, 2004Co-Authors: M Tamassia, F Nuttinck, Pascale Maypanloup, Pascal Reynier, Y Heyman, Gilles Charpigny, Miodrag Stojkovic, S Hiendleder, J P Renard, S ChastantmaillardAbstract:Mitochondria have a broad range of functions that affect reproduction, and structural as well as quantitative variation in mtDNA has been associated with gamete quality and reproductive success. To investigate the mitochondria effect on in vitro embryo production, we collected oocytes by ultrasound-guided Follicular Aspiration from donor cows known to differ in the developmental capacity, measured by the blastocyst formation rate, of their oocytes. To evaluate the potential effects of mtDNA and mitochondrial function on oocyte quality, the donor cows' mtDNA control region was sequenced and, after pairwise comparisons of polymorphisms, animals were grouped into two major haplogroups. The number of mtDNA molecules per oocyte was quantified by real-time PCR, and the adenosine triphosphate (ATP) content was measured in each oocyte to identify variations between haplogroups. Overall, ATP stocks in oocytes of the two haplogroups differed significantly (P < 0.05; means +/- SEM) both at the germinal vesicle and metaphase II stages (2.8 +/- 0.06 pmol vs. 2.6 +/- 0.07 pmol and 2.9 +/- 0.1 pmol vs. 2.3 +/- 0.06 pmol, respectively). The proportion of development to blastocyst was significantly different between haplogroups (22.3 +/- 2.1 % vs. 36.7 +/- 2.9 %). The number of mtDNA molecules per oocyte was highly variable (377 327 +/- 14 104, ranging from 2.0 x 10(3) to 1.2 x 10(6)) but not significantly different between the two haplogroups; significant differences were observed between animals without any apparent relationship to blastocyst production. These data suggest that mitochondria and mtDNA haplogroup affect the developmental capacity of bovine oocytes in vitro.
Gilles Charpigny - One of the best experts on this subject based on the ideXlab platform.
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in vitro embryo production efficiency in cattle and its association with oocyte adenosine triphosphate content quantity of mitochondrial dna and mitochondrial dna haplogroup
Biology of Reproduction, 2004Co-Authors: M Tamassia, F Nuttinck, Pascale Maypanloup, Pascal Reynier, Y Heyman, Gilles Charpigny, Miodrag Stojkovic, S Hiendleder, J P Renard, S ChastantmaillardAbstract:Abstract Mitochondria have a broad range of functions that affect reproduction, and structural as well as quantitative variation in mtDNA has been associated with gamete quality and reproductive success. To investigate the mitochondria effect on in vitro embryo production, we collected oocytes by ultrasound-guided Follicular Aspiration from donor cows known to differ in the developmental capacity, measured by the blastocyst formation rate, of their oocytes. To evaluate the potential effects of mtDNA and mitochondrial function on oocyte quality, the donor cows' mtDNA control region was sequenced and, after pairwise comparisons of polymorphisms, animals were grouped into two major haplogroups. The number of mtDNA molecules per oocyte was quantified by real-time PCR, and the adenosine triphosphate (ATP) content was measured in each oocyte to identify variations between haplogroups. Overall, ATP stocks in oocytes of the two haplogroups differed significantly (P < 0.05; means ± SEM) both at the germinal vesicl...
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in vitro embryo production efficiency in cattle and its association with oocyte adenosine triphosphate content quantity of mitochondrial dna and mitochondrial dna haplogroup
Biology of Reproduction, 2004Co-Authors: M Tamassia, F Nuttinck, Pascale Maypanloup, Pascal Reynier, Y Heyman, Gilles Charpigny, Miodrag Stojkovic, S Hiendleder, J P Renard, S ChastantmaillardAbstract:Mitochondria have a broad range of functions that affect reproduction, and structural as well as quantitative variation in mtDNA has been associated with gamete quality and reproductive success. To investigate the mitochondria effect on in vitro embryo production, we collected oocytes by ultrasound-guided Follicular Aspiration from donor cows known to differ in the developmental capacity, measured by the blastocyst formation rate, of their oocytes. To evaluate the potential effects of mtDNA and mitochondrial function on oocyte quality, the donor cows' mtDNA control region was sequenced and, after pairwise comparisons of polymorphisms, animals were grouped into two major haplogroups. The number of mtDNA molecules per oocyte was quantified by real-time PCR, and the adenosine triphosphate (ATP) content was measured in each oocyte to identify variations between haplogroups. Overall, ATP stocks in oocytes of the two haplogroups differed significantly (P < 0.05; means +/- SEM) both at the germinal vesicle and metaphase II stages (2.8 +/- 0.06 pmol vs. 2.6 +/- 0.07 pmol and 2.9 +/- 0.1 pmol vs. 2.3 +/- 0.06 pmol, respectively). The proportion of development to blastocyst was significantly different between haplogroups (22.3 +/- 2.1 % vs. 36.7 +/- 2.9 %). The number of mtDNA molecules per oocyte was highly variable (377 327 +/- 14 104, ranging from 2.0 x 10(3) to 1.2 x 10(6)) but not significantly different between the two haplogroups; significant differences were observed between animals without any apparent relationship to blastocyst production. These data suggest that mitochondria and mtDNA haplogroup affect the developmental capacity of bovine oocytes in vitro.