The Experts below are selected from a list of 14577 Experts worldwide ranked by ideXlab platform
Chris A Liacouras - One of the best experts on this subject based on the ideXlab platform.
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Food Allergen triggers are increased in children with the tslp risk allele and eosinophilic esophagitis
Clinical and translational gastroenterology, 2018Co-Authors: Lisa M Fahey, Prasanna M Chandramouleeswaran, Shaobo Guan, Alain Benitez, Glenn T Furuta, Seema S Aceves, Meilun Wang, Chris A LiacourasAbstract:OBJECTIVES TSLP has been shown to be associated with eosinophilic esophagitis (EoE). Specifically, children with EoE often have the nucleotides AA or AG instead of GG at the single nucleotide polymorphism position RS3806932. Presently, the phenotypic characteristics in EoE children with the TSLP EoE risk allele are unknown. METHODS A retrospective analysis was performed of all children with EoE who had TSLP genotyping at The Children's Hospital of Philadelphia from 2008-2014. EoE Food Allergen triggers, presence of atopic features, IgE mediated Food allergy and skin prick testing results were reviewed. The number and type of EoE Food Allergen triggers were compared with genotype using chi-square analysis. Primary cell cultures from EoE patients with or without the risk allele were stimulated with ovalbumin and TSLP secretion was measured by ELISA. RESULTS Fifty three of 309 patients were found to have no copies of the TSLP risk allele, whereas 256 patients were found to have one or more copies of the risk allele. There was an increase in the number of patients with three or more EoE Food Allergens among those who were either homozygous or heterozygous for the risk allele compared to those without the risk allele (P < 0.0001). This was independent of their atopic background. Primary cultures from patients homozygous for the risk allele had greater TSLP secretion than those isolated from heterozygous patients. CONCLUSIONS The TSLP risk allele is associated with having multiple EoE Food Allergen triggers. This novel EoE genotypic-phenotypic correlation may guide future treatment for those with the TSLP risk allele.
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Food Allergen triggers are increased in children with the TSLP risk allele and eosinophilic esophagitis
Clinical and Translational Gastroenterology, 2018Co-Authors: Lisa M Fahey, Prasanna M Chandramouleeswaran, Shaobo Guan, Glenn T Furuta, Seema S Aceves, Meilun Wang, Chris A Liacouras, Alain J. Benitez, Amanda B. Muir, Patrick Ma SleimanAbstract:Objectives TSLP has been shown to be associated with eosinophilic esophagitis (EoE). Specifically, children with EoE often have the nucleotides AA or AG instead of GG at the single nucleotide polymorphism position RS3806932. Presently, the phenotypic characteristics in EoE children with the TSLP EoE risk allele are unknown. Methods A retrospective analysis was performed of all children with EoE who had TSLP genotyping at The Children’s Hospital of Philadelphia from 2008–2014. EoE Food Allergen triggers, presence of atopic features, IgE mediated Food allergy and skin prick testing results were reviewed. The number and type of EoE Food Allergen triggers were compared with genotype using chi-square analysis. Primary cell cultures from EoE patients with or without the risk allele were stimulated with ovalbumin and TSLP secretion was measured by ELISA. Results Fifty three of 309 patients were found to have no copies of the TSLP risk allele, whereas 256 patients were found to have one or more copies of the risk allele. There was an increase in the number of patients with three or more EoE Food Allergens among those who were either homozygous or heterozygous for the risk allele compared to those without the risk allele ( P
Lisa M Fahey - One of the best experts on this subject based on the ideXlab platform.
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Food Allergen triggers are increased in children with the tslp risk allele and eosinophilic esophagitis
Clinical and translational gastroenterology, 2018Co-Authors: Lisa M Fahey, Prasanna M Chandramouleeswaran, Shaobo Guan, Alain Benitez, Glenn T Furuta, Seema S Aceves, Meilun Wang, Chris A LiacourasAbstract:OBJECTIVES TSLP has been shown to be associated with eosinophilic esophagitis (EoE). Specifically, children with EoE often have the nucleotides AA or AG instead of GG at the single nucleotide polymorphism position RS3806932. Presently, the phenotypic characteristics in EoE children with the TSLP EoE risk allele are unknown. METHODS A retrospective analysis was performed of all children with EoE who had TSLP genotyping at The Children's Hospital of Philadelphia from 2008-2014. EoE Food Allergen triggers, presence of atopic features, IgE mediated Food allergy and skin prick testing results were reviewed. The number and type of EoE Food Allergen triggers were compared with genotype using chi-square analysis. Primary cell cultures from EoE patients with or without the risk allele were stimulated with ovalbumin and TSLP secretion was measured by ELISA. RESULTS Fifty three of 309 patients were found to have no copies of the TSLP risk allele, whereas 256 patients were found to have one or more copies of the risk allele. There was an increase in the number of patients with three or more EoE Food Allergens among those who were either homozygous or heterozygous for the risk allele compared to those without the risk allele (P < 0.0001). This was independent of their atopic background. Primary cultures from patients homozygous for the risk allele had greater TSLP secretion than those isolated from heterozygous patients. CONCLUSIONS The TSLP risk allele is associated with having multiple EoE Food Allergen triggers. This novel EoE genotypic-phenotypic correlation may guide future treatment for those with the TSLP risk allele.
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Food Allergen triggers are increased in children with the TSLP risk allele and eosinophilic esophagitis
Clinical and Translational Gastroenterology, 2018Co-Authors: Lisa M Fahey, Prasanna M Chandramouleeswaran, Shaobo Guan, Glenn T Furuta, Seema S Aceves, Meilun Wang, Chris A Liacouras, Alain J. Benitez, Amanda B. Muir, Patrick Ma SleimanAbstract:Objectives TSLP has been shown to be associated with eosinophilic esophagitis (EoE). Specifically, children with EoE often have the nucleotides AA or AG instead of GG at the single nucleotide polymorphism position RS3806932. Presently, the phenotypic characteristics in EoE children with the TSLP EoE risk allele are unknown. Methods A retrospective analysis was performed of all children with EoE who had TSLP genotyping at The Children’s Hospital of Philadelphia from 2008–2014. EoE Food Allergen triggers, presence of atopic features, IgE mediated Food allergy and skin prick testing results were reviewed. The number and type of EoE Food Allergen triggers were compared with genotype using chi-square analysis. Primary cell cultures from EoE patients with or without the risk allele were stimulated with ovalbumin and TSLP secretion was measured by ELISA. Results Fifty three of 309 patients were found to have no copies of the TSLP risk allele, whereas 256 patients were found to have one or more copies of the risk allele. There was an increase in the number of patients with three or more EoE Food Allergens among those who were either homozygous or heterozygous for the risk allele compared to those without the risk allele ( P
Michel W F Nielen - One of the best experts on this subject based on the ideXlab platform.
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Consumer-friendly Food Allergen detection: moving towards smartphone-based immunoassays
Analytical and Bioanalytical Chemistry, 2018Co-Authors: Georgina M. S. Ross, Monique G. E. G. Bremer, Michel W F NielenAbstract:In this critical review, we provide a comprehensive overview of immunochemical Food Allergen assays and detectors in the context of their user-friendliness, through their connection to smartphones. Smartphone-based analysis is centered around citizen science, putting analysis into the hands of the consumer. Food allergies represent a significant worldwide health concern and consumers should be able to analyze their Foods, whenever and wherever they are, for Allergen presence. Owing to the need for a scientific background, traditional laboratory-based detection methods are generally unsuitable for the consumer. Therefore, it is important to develop simple, safe, and rapid assays that can be linked with smartphones as detectors to improve user accessibility. Smartphones make excellent detection systems because of their cameras, embedded flash functions, portability, connectivity, and affordability. Therefore, this review has summarized traditional laboratory-based methods for Food Allergen detection such as enzyme-linked-immunosorbent assay, flow cytometry, and surface plasmon resonance, and the potential to modernize these methods by interfacing them with a smartphone readout system, based on the aforementioned smartphone characteristics. This is the first review focusing on smartphone-based Food-Allergen detection methods designed with the intention of being consumer-friendly. Graphical abstractA smartphone-based Food Allergen detection system in three easy steps (1) sample preparation, (2) Allergen detection on a smartphone using antibodies, which then transmits the data wirelessly, (3) analytical results sent straight to smartphone
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consumer friendly Food Allergen detection moving towards smartphone based immunoassays
Analytical and Bioanalytical Chemistry, 2018Co-Authors: Georgina M. S. Ross, Monique Bremer, Michel W F NielenAbstract:In this critical review, we provide a comprehensive overview of immunochemical Food Allergen assays and detectors in the context of their user-friendliness, through their connection to smartphones. Smartphone-based analysis is centered around citizen science, putting analysis into the hands of the consumer. Food allergies represent a significant worldwide health concern and consumers should be able to analyze their Foods, whenever and wherever they are, for Allergen presence. Owing to the need for a scientific background, traditional laboratory-based detection methods are generally unsuitable for the consumer. Therefore, it is important to develop simple, safe, and rapid assays that can be linked with smartphones as detectors to improve user accessibility. Smartphones make excellent detection systems because of their cameras, embedded flash functions, portability, connectivity, and affordability. Therefore, this review has summarized traditional laboratory-based methods for Food Allergen detection such as enzyme-linked-immunosorbent assay, flow cytometry, and surface plasmon resonance, and the potential to modernize these methods by interfacing them with a smartphone readout system, based on the aforementioned smartphone characteristics. This is the first review focusing on smartphone-based Food-Allergen detection methods designed with the intention of being consumer-friendly.
Patrick Ma Sleiman - One of the best experts on this subject based on the ideXlab platform.
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Food Allergen triggers are increased in children with the TSLP risk allele and eosinophilic esophagitis
Clinical and Translational Gastroenterology, 2018Co-Authors: Lisa M Fahey, Prasanna M Chandramouleeswaran, Shaobo Guan, Glenn T Furuta, Seema S Aceves, Meilun Wang, Chris A Liacouras, Alain J. Benitez, Amanda B. Muir, Patrick Ma SleimanAbstract:Objectives TSLP has been shown to be associated with eosinophilic esophagitis (EoE). Specifically, children with EoE often have the nucleotides AA or AG instead of GG at the single nucleotide polymorphism position RS3806932. Presently, the phenotypic characteristics in EoE children with the TSLP EoE risk allele are unknown. Methods A retrospective analysis was performed of all children with EoE who had TSLP genotyping at The Children’s Hospital of Philadelphia from 2008–2014. EoE Food Allergen triggers, presence of atopic features, IgE mediated Food allergy and skin prick testing results were reviewed. The number and type of EoE Food Allergen triggers were compared with genotype using chi-square analysis. Primary cell cultures from EoE patients with or without the risk allele were stimulated with ovalbumin and TSLP secretion was measured by ELISA. Results Fifty three of 309 patients were found to have no copies of the TSLP risk allele, whereas 256 patients were found to have one or more copies of the risk allele. There was an increase in the number of patients with three or more EoE Food Allergens among those who were either homozygous or heterozygous for the risk allele compared to those without the risk allele ( P
Alain Benitez - One of the best experts on this subject based on the ideXlab platform.
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Food Allergen triggers are increased in children with the tslp risk allele and eosinophilic esophagitis
Clinical and translational gastroenterology, 2018Co-Authors: Lisa M Fahey, Prasanna M Chandramouleeswaran, Shaobo Guan, Alain Benitez, Glenn T Furuta, Seema S Aceves, Meilun Wang, Chris A LiacourasAbstract:OBJECTIVES TSLP has been shown to be associated with eosinophilic esophagitis (EoE). Specifically, children with EoE often have the nucleotides AA or AG instead of GG at the single nucleotide polymorphism position RS3806932. Presently, the phenotypic characteristics in EoE children with the TSLP EoE risk allele are unknown. METHODS A retrospective analysis was performed of all children with EoE who had TSLP genotyping at The Children's Hospital of Philadelphia from 2008-2014. EoE Food Allergen triggers, presence of atopic features, IgE mediated Food allergy and skin prick testing results were reviewed. The number and type of EoE Food Allergen triggers were compared with genotype using chi-square analysis. Primary cell cultures from EoE patients with or without the risk allele were stimulated with ovalbumin and TSLP secretion was measured by ELISA. RESULTS Fifty three of 309 patients were found to have no copies of the TSLP risk allele, whereas 256 patients were found to have one or more copies of the risk allele. There was an increase in the number of patients with three or more EoE Food Allergens among those who were either homozygous or heterozygous for the risk allele compared to those without the risk allele (P < 0.0001). This was independent of their atopic background. Primary cultures from patients homozygous for the risk allele had greater TSLP secretion than those isolated from heterozygous patients. CONCLUSIONS The TSLP risk allele is associated with having multiple EoE Food Allergen triggers. This novel EoE genotypic-phenotypic correlation may guide future treatment for those with the TSLP risk allele.