The Experts below are selected from a list of 87456 Experts worldwide ranked by ideXlab platform

Attila Nagy - One of the best experts on this subject based on the ideXlab platform.

Keir C. Neuman - One of the best experts on this subject based on the ideXlab platform.

Andre Marziali - One of the best experts on this subject based on the ideXlab platform.

  • single molecule bonds characterized by solid state nanopore Force Spectroscopy
    ACS Nano, 2009
    Co-Authors: Vincent Tabardcossa, Matthew Wiggin, Dhruti Trivedi, Nahid N Jetha, Jason R Dwyer, Andre Marziali
    Abstract:

    Weak molecular interactions drive processes at the core of living systems, such as enzyme−substrate interactions, receptor−ligand binding, and nucleic acid replication. Single-molecule Force Spectroscopy is a remarkable tool for revealing molecular scale energy landscapes of noncovalent bonds, by exerting a mechanical Force directly on an individual molecular complex and tracking its survival as a function of time and applied Force. In principle, Force Spectroscopy methods can also be used for highly specific molecular recognition assays, by directly characterizing the strength of bonds between probe and target molecules. However, complexity and low throughput of conventional Force Spectroscopy techniques render such biosensing applications impractical. Here we demonstrate a straightforward single-molecule approach, suitable for both biophysical studies and molecular recognition assays, in which a ∼3 nm silicon nitride nanopore is used to determine the bond lifetime spectrum of the biotin−neutravidin comp...

  • Nanopore Force Spectroscopy on DNA duplexes.
    Methods in molecular biology (Clifton N.J.), 2009
    Co-Authors: Nahid N Jetha, Matthew Wiggin, Andre Marziali
    Abstract:

    Force Spectroscopy can be applied using nanopores to study charged molecules such as nucleic acids. This technique can be used to study the binding energy of a DNA duplex by threading an anchored single-stranded DNA (ssDNA) probe molecule through a nanopore (having a diameter large enough to accommodate only a single strand) and allowing target DNA on the backside of the pore to hybridize to the probe. Electric potential can be used to apply a Force to the charged ssDNA in a direction tending to translocate the duplex through the pore. If the pore is only large enough to accept ssDNA, the duplex must dissociate for the probe to escape the pore. The dissociation time of the duplex can therefore be measured under applied Force, and (provided that enough dissociation events have been recorded) a characteristic time scale for dissociation can be determined. In this chapter, we present a detailed protocol for performing nanopore Force Spectroscopy on DNA duplexes using one or more alpha-hemolysin nanopores. We present the details of the measurement of the duplex survival probability under Force, and show that dissociation time scales for duplexes that are perfectly complimentary differ by greater than approximately two orders of magnitude from those containing a single sequence mismatch, offering opportunities for sequence detection.

  • Multi-Nanopore Force Spectroscopy for DNA Analysis
    Biophysical journal, 2006
    Co-Authors: Carolina Tropini, Andre Marziali
    Abstract:

    The need for low-cost DNA sequence detection in clinical applications is driving development of new technologies. We demonstrate a method for detection of mutations in a DNA sequence purely by electronic means, and without need for fluorescent labeling. Our method uses an array of nanopores to perform synchronized single-molecule Force Spectroscopy measurements over many molecules in parallel, yielding detailed information on the kinetics of hundreds of molecule dissociations in a single measurement.

Daniel J. Müller - One of the best experts on this subject based on the ideXlab platform.

  • Single-Molecule Force Spectroscopy of Transmembrane β-Barrel Proteins.
    Annual review of analytical chemistry (Palo Alto Calif.), 2018
    Co-Authors: Johannes Thoma, K. Tanuj Sapra, Daniel J. Müller
    Abstract:

    Single-molecule Force Spectroscopy (SMFS) has been widely applied to study the mechanical unfolding and folding of transmembrane proteins. Here, we review the recent progress in characterizing bact...

  • Quantifying cellular adhesion to extracellular matrix components by single-cell Force Spectroscopy
    Nature Protocols, 2010
    Co-Authors: Jens Friedrichs, Jonne Helenius, Daniel J. Müller
    Abstract:

    Quantifying cellular adhesion to extracellular matrix components by single-cell Force Spectroscopy

  • single cell Force Spectroscopy
    Journal of Cell Science, 2008
    Co-Authors: Jonne Helenius, Hermann E. Gaub, Carlphilipp Heisenberg, Daniel J. Müller
    Abstract:

    The controlled adhesion of cells to each other and to the extracellular matrix is crucial for tissue development and maintenance. Numerous assays have been developed to quantify cell adhesion. Among these, the use of atomic Force microscopy (AFM) for single-cell Force Spectroscopy (SCFS) has recently been established. This assay permits the adhesion of living cells to be studied in near-physiological conditions. This implementation of AFM allows unrivaled spatial and temporal control of cells, as well as highly quantitative Force actuation and Force measurement that is sufficiently sensitive to characterize the interaction of single molecules. Therefore, not only overall cell adhesion but also the properties of single adhesion-receptor–ligand interactions can be studied. Here we describe current implementations and applications of SCFS, as well as potential pitfalls, and outline how developments will provide insight into the Forces, energetics and kinetics of celladhesion processes.

Lawrence A. Bottomley - One of the best experts on this subject based on the ideXlab platform.

  • Plastic tip arrays for Force Spectroscopy.
    Analytical Chemistry, 2004
    Co-Authors: Peter T. Lillehei, Mark A. Poggi, Brian J. Polk, And J. Anthony Smith, Lawrence A. Bottomley
    Abstract:

    The mechanical stability and viability of molecules investigated with the atomic Force microscope (AFM) continue to be limiting factors in the duration of Force Spectroscopy measurements. In an eff...

  • Plastic tip arrays for Force Spectroscopy.
    Analytical chemistry, 2004
    Co-Authors: Peter T. Lillehei, Mark A. Poggi, Brian J. Polk, And J. Anthony Smith, Lawrence A. Bottomley
    Abstract:

    The mechanical stability and viability of molecules investigated with the atomic Force microscope (AFM) continue to be limiting factors in the duration of Force Spectroscopy measurements. In an effort to circumvent this problem, we have fabricated an all-plastic array of over 30 000 tips with dimensions similar to common AFM probes using silicon micromolding techniques. This approach enables rapid fabrication of tip arrays with improved properties, as compared to tip arrays made entirely of silicon.