Formylation

14,000,000 Leading Edge Experts on the ideXlab platform

Scan Science and Technology

Contact Leading Edge Experts & Companies

Scan Science and Technology

Contact Leading Edge Experts & Companies

The Experts below are selected from a list of 2607 Experts worldwide ranked by ideXlab platform

Carlos T Moraes - One of the best experts on this subject based on the ideXlab platform.

  • mitochondrial methionyl n Formylation affects steady state levels of oxidative phosphorylation complexes and their organization into supercomplexes
    Journal of Biological Chemistry, 2018
    Co-Authors: Tania Arguello, Caroline Kohrer, Uttam L Rajbhandary, Carlos T Moraes
    Abstract:

    N-Formylation of the Met-tRNAMet by the nuclearly encoded mitochondrial methionyl-tRNA formyltransferase (MTFMT) has been found to be a key determinant of protein synthesis initiation in mitochondria. In humans, mutations in the MTFMT gene result in Leigh syndrome, a progressive and severe neurometabolic disorder. However, the absolute requirement of Formylation of Met-tRNAMet for protein synthesis in mammalian mitochondria is still debated. Here, we generated a Mtfmt-KO mouse fibroblast cell line and demonstrated that N-Formylation of the first methionine via fMet-tRNAMet by MTFMT is not an absolute requirement for initiation of protein synthesis. However, it differentially affected the efficiency of synthesis of mtDNA-coded polypeptides. Lack of methionine N-Formylation did not compromise the stability of these individual subunits but had a marked effect on the assembly and stability of the OXPHOS complexes I and IV and on their supercomplexes. In summary, N-Formylation is not essential for mitochondrial protein synthesis but is critical for efficient synthesis of several mitochondrially encoded peptides and for OXPHOS complex stability and assembly into supercomplexes.

Krishnacharya G Akamanchi - One of the best experts on this subject based on the ideXlab platform.

Tania Arguello - One of the best experts on this subject based on the ideXlab platform.

  • mitochondrial methionyl n Formylation affects steady state levels of oxidative phosphorylation complexes and their organization into supercomplexes
    Journal of Biological Chemistry, 2018
    Co-Authors: Tania Arguello, Caroline Kohrer, Uttam L Rajbhandary, Carlos T Moraes
    Abstract:

    N-Formylation of the Met-tRNAMet by the nuclearly encoded mitochondrial methionyl-tRNA formyltransferase (MTFMT) has been found to be a key determinant of protein synthesis initiation in mitochondria. In humans, mutations in the MTFMT gene result in Leigh syndrome, a progressive and severe neurometabolic disorder. However, the absolute requirement of Formylation of Met-tRNAMet for protein synthesis in mammalian mitochondria is still debated. Here, we generated a Mtfmt-KO mouse fibroblast cell line and demonstrated that N-Formylation of the first methionine via fMet-tRNAMet by MTFMT is not an absolute requirement for initiation of protein synthesis. However, it differentially affected the efficiency of synthesis of mtDNA-coded polypeptides. Lack of methionine N-Formylation did not compromise the stability of these individual subunits but had a marked effect on the assembly and stability of the OXPHOS complexes I and IV and on their supercomplexes. In summary, N-Formylation is not essential for mitochondrial protein synthesis but is critical for efficient synthesis of several mitochondrially encoded peptides and for OXPHOS complex stability and assembly into supercomplexes.

Ravinder Singh Kuntal - One of the best experts on this subject based on the ideXlab platform.

  • comparative study on feed formulation software a short review
    International Journal of Research, 2017
    Co-Authors: Vishal Patil, R Gupta, D Rajendran, Ravinder Singh Kuntal
    Abstract:

    The paper highlightsthe different feed formulation software’s for least cost formulation of livestock and animals, where the comparison of techniques is donebased on different aspectslike data collection, software used, price, scope and limitations. Feed formulation requires large amount of data about the composition of feeds, environment conditions,and availability of feeds.Development of Software’sfor feed formulations is changing with change in computer.  With advancement of technology, the feed formulation can be calculated for ‘n’ number of feed stuff but still Linear Programming is used as base for mathematical  modelling, where uses of non-linear programming is very less. Maximum number of feed formulation software’s is developed to select the feeds for formulation within budget of the farmers or dairy industry.

Sagar P Pathare - One of the best experts on this subject based on the ideXlab platform.