The Experts below are selected from a list of 126 Experts worldwide ranked by ideXlab platform
Y F Yuan - One of the best experts on this subject based on the ideXlab platform.
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Foundational Study of subcritical water chromatography
Acta pharmaceutica Sinica, 2000Co-Authors: P Sun, Y F YuanAbstract:AIM To Study the subcritical water chromatographic discipline of compounds with medium and high polarity on the capillary and packed column. METHODS A packed and capillary subcritical water chromatography FID detection system was built up. Six alcohols were well separated on a C 18 column and the influence of temperature on chromatographic behavior of alcohols with different polarity was studied. The influence of temperature and pressure on chromatographic behavior of benzoic acid and gensenoside Rg1 on a SE 54 capillary column were also studied. RESULTS Increasing temperature resulted in the decreasing polarity of mobile phase, shortened retention times of components separated, narrowed peak shape and improved tailing as well. CONCLUSION Temperature programming could be used successfully to separate compounds with various polarity selectively. Thermostable capillary column could enlarge the range of temperature even more.
Chintamani D. Atreya - One of the best experts on this subject based on the ideXlab platform.
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A Foundational Study for Normal F8-Containing Mouse Models for the miRNA Regulation of Hemophilia A: Identification and Analysis of Mouse miRNAs that Downregulate the Murine F8 Gene.
International journal of molecular sciences, 2020Co-Authors: Katarzyna I. Jankowska, Maitreyi Chattopadhyay, Zuben E. Sauna, Chintamani D. AtreyaAbstract:Hemophilia A (HA) is associated with defects in the F8 gene, encoding coagulation factor VIII (FVIII). Our previous studies show that F8-targeting micro RNAs (miRNAs), a group of small RNAs involved in gene regulation, can downregulate F8 expression causing HA in individuals with normal F8-genotypes and increased HA severity in patients with mutations in F8. Understanding the mechanistic underpinnings of human genetic diseases caused or modulated by miRNAs require a small animal model, such as a mouse model. Here, we report a Foundational Study to develop such a model system. We identified the mouse 3′untranslated region (3′UTR) on murine F8-mRNA (muF8-mRNA) that can bind to murine miRNAs. We then selected three miRNAs for evaluation: miR-208a, miR-351 and miR-125a. We first demonstrate that these three miRNAs directly target the 3′UTR of muF8-mRNA and reduce the expression of a reporter gene (luciferase) mRNA fused to the muF8-3′ UTR in mammalian cells. Furthermore, in mouse cells that endogenously express the F8 gene and produce FVIII protein, the ectopic expression of these miRNAs downregulated F8-mRNA and FVIII protein. These results provide proof-of-concept and reagents as a foundation for using a normal F8-containing mouse as a model for the miRNA regulation of normal F8 in causing or aggravating the genetic disease HA.
P Sun - One of the best experts on this subject based on the ideXlab platform.
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Foundational Study of subcritical water chromatography
Acta pharmaceutica Sinica, 2000Co-Authors: P Sun, Y F YuanAbstract:AIM To Study the subcritical water chromatographic discipline of compounds with medium and high polarity on the capillary and packed column. METHODS A packed and capillary subcritical water chromatography FID detection system was built up. Six alcohols were well separated on a C 18 column and the influence of temperature on chromatographic behavior of alcohols with different polarity was studied. The influence of temperature and pressure on chromatographic behavior of benzoic acid and gensenoside Rg1 on a SE 54 capillary column were also studied. RESULTS Increasing temperature resulted in the decreasing polarity of mobile phase, shortened retention times of components separated, narrowed peak shape and improved tailing as well. CONCLUSION Temperature programming could be used successfully to separate compounds with various polarity selectively. Thermostable capillary column could enlarge the range of temperature even more.
Katarzyna I. Jankowska - One of the best experts on this subject based on the ideXlab platform.
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A Foundational Study for Normal F8-Containing Mouse Models for the miRNA Regulation of Hemophilia A: Identification and Analysis of Mouse miRNAs that Downregulate the Murine F8 Gene.
International journal of molecular sciences, 2020Co-Authors: Katarzyna I. Jankowska, Maitreyi Chattopadhyay, Zuben E. Sauna, Chintamani D. AtreyaAbstract:Hemophilia A (HA) is associated with defects in the F8 gene, encoding coagulation factor VIII (FVIII). Our previous studies show that F8-targeting micro RNAs (miRNAs), a group of small RNAs involved in gene regulation, can downregulate F8 expression causing HA in individuals with normal F8-genotypes and increased HA severity in patients with mutations in F8. Understanding the mechanistic underpinnings of human genetic diseases caused or modulated by miRNAs require a small animal model, such as a mouse model. Here, we report a Foundational Study to develop such a model system. We identified the mouse 3′untranslated region (3′UTR) on murine F8-mRNA (muF8-mRNA) that can bind to murine miRNAs. We then selected three miRNAs for evaluation: miR-208a, miR-351 and miR-125a. We first demonstrate that these three miRNAs directly target the 3′UTR of muF8-mRNA and reduce the expression of a reporter gene (luciferase) mRNA fused to the muF8-3′ UTR in mammalian cells. Furthermore, in mouse cells that endogenously express the F8 gene and produce FVIII protein, the ectopic expression of these miRNAs downregulated F8-mRNA and FVIII protein. These results provide proof-of-concept and reagents as a foundation for using a normal F8-containing mouse as a model for the miRNA regulation of normal F8 in causing or aggravating the genetic disease HA.
Zhiyong Qian - One of the best experts on this subject based on the ideXlab platform.
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microneedles based transdermal drug delivery systems a review
Journal of Biomedical Nanotechnology, 2017Co-Authors: Wei Li, Xingli Zhou, Fan Yang, Zhiyong QianAbstract:: A microneedle (MN) is a micron-sized needle with a height of 10-2000 μm and a width of 10-50 μm, which can penetrate through the epidermis layer to dermal tissue directly without pain. Microneedles are widely used in transdermal drug delivery systems (TDDS) because they are efficient, safe, convenient and painless. Morphologically, microneedles are divided into four types: solid microneedles, coated microneedles, dissolving microneedles, and hollow microneedles. Different types of microneedles play different roles in different research fields. Microneedles also have different characteristics and applications depending on the materials they are made from. In recent years, microneedles have frequently been used to deliver drugs, genes, proteins, RNA, and vaccines, and have achieved amazing therapeutic effect. Meanwhile, a variety of nano-carriers combined with microneedle delivery systems highlight the application of microneedles. The materials, types, and applications of the microneedles are summarized in this review. Overall, this review aims to serve as a Foundational Study of microneedles and hopes to promote their clinical application.