The Experts below are selected from a list of 1839 Experts worldwide ranked by ideXlab platform
Ben Adler - One of the best experts on this subject based on the ideXlab platform.
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Screening of 71 P. multocida proteins for protective efficacy in a Fowl Cholera infection model and characterization of the protective antigen PlpE
PloS one, 2012Co-Authors: Tamas Zsolt Hatfaludi, Keith Al-hasani, Lan Gong, John D. Boyce, Mark Ford, Ian W Wilkie, Noelene Sheila Quinsey, Michelle A. Dunstone, David E. Hoke, Ben AdlerAbstract:Background: There is a strong need for a recombinant subunit vaccine against Fowl Cholera. We used a reverse vaccinology approach to identify putative secreted or cell surface associated P. multocida proteins that may represent potential vaccine candidate antigens. Principal Findings: A high-throughput cloning and expression protocol was used to express and purify 71 recombinant proteins for vaccine trials. Of the 71 proteins tested, only one, PlpE in denatured insoluble form, protected chickens against Fowl Cholera challenge. PlpE also elicited comparable levels of protection in mice. PlpE was localized by immunofluorescence to the bacterial cell surface, consistent with its ability to elicit a protective immune response. To explore the role of PlpE during infection and immunity, a plpE mutant was generated. The plpE mutant strain retained full virulence for mice. Conclusion: These studies show that PlpE is a surface exposed protein and was the only protein of 71 tested that was able to elicit a protective immune response. However, PlpE is not an essential virulence factor. This is the first report of a denatured recombinant protein stimulating protection against Fowl Cholera.
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Vaccination against Fowl Cholera with acapsular Pasteurella multocida A:1.
Vaccine, 2005Co-Authors: Jing Yeng Chung, John D. Boyce, Ian W Wilkie, Ben AdlerAbstract:We have previously constructed an acapsular Pasteurella multocida X-73 (serogroup A) mutant strain which was attenuated in virulence for chickens (Chung JY, Wilkie IW, Boyce JD, Townsend KM, Frost AJ, Ghodussi M, Adler B. Role of capsule in the pathogenesis of Fowl Cholera caused by Pasteurella multocida serogroup A. Infect. Immun. 2001;69:2487-2492). In this study, we have assessed the ability of this acapsular strain (PBA930) to induce protection against wild-type challenge in mice and the natural host chickens. Intramuscular administration of PBA930 to mice stimulated significant protection against X-73 and the heterologous strain P-1059 (A:3), but not against challenge with P-1662 (A:4). No protection was observed when PBA930 was introduced by the intraperitoneal or subcutaneous routes in mice. Significantly, the acapsular strain PBA930 was able to induce protection against challenge with wild type X-73 in chickens.
Ian W Wilkie - One of the best experts on this subject based on the ideXlab platform.
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Screening of 71 P. multocida proteins for protective efficacy in a Fowl Cholera infection model and characterization of the protective antigen PlpE
PloS one, 2012Co-Authors: Tamas Zsolt Hatfaludi, Keith Al-hasani, Lan Gong, John D. Boyce, Mark Ford, Ian W Wilkie, Noelene Sheila Quinsey, Michelle A. Dunstone, David E. Hoke, Ben AdlerAbstract:Background: There is a strong need for a recombinant subunit vaccine against Fowl Cholera. We used a reverse vaccinology approach to identify putative secreted or cell surface associated P. multocida proteins that may represent potential vaccine candidate antigens. Principal Findings: A high-throughput cloning and expression protocol was used to express and purify 71 recombinant proteins for vaccine trials. Of the 71 proteins tested, only one, PlpE in denatured insoluble form, protected chickens against Fowl Cholera challenge. PlpE also elicited comparable levels of protection in mice. PlpE was localized by immunofluorescence to the bacterial cell surface, consistent with its ability to elicit a protective immune response. To explore the role of PlpE during infection and immunity, a plpE mutant was generated. The plpE mutant strain retained full virulence for mice. Conclusion: These studies show that PlpE is a surface exposed protein and was the only protein of 71 tested that was able to elicit a protective immune response. However, PlpE is not an essential virulence factor. This is the first report of a denatured recombinant protein stimulating protection against Fowl Cholera.
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Vaccination against Fowl Cholera with acapsular Pasteurella multocida A:1.
Vaccine, 2005Co-Authors: Jing Yeng Chung, John D. Boyce, Ian W Wilkie, Ben AdlerAbstract:We have previously constructed an acapsular Pasteurella multocida X-73 (serogroup A) mutant strain which was attenuated in virulence for chickens (Chung JY, Wilkie IW, Boyce JD, Townsend KM, Frost AJ, Ghodussi M, Adler B. Role of capsule in the pathogenesis of Fowl Cholera caused by Pasteurella multocida serogroup A. Infect. Immun. 2001;69:2487-2492). In this study, we have assessed the ability of this acapsular strain (PBA930) to induce protection against wild-type challenge in mice and the natural host chickens. Intramuscular administration of PBA930 to mice stimulated significant protection against X-73 and the heterologous strain P-1059 (A:3), but not against challenge with P-1662 (A:4). No protection was observed when PBA930 was introduced by the intraperitoneal or subcutaneous routes in mice. Significantly, the acapsular strain PBA930 was able to induce protection against challenge with wild type X-73 in chickens.
John D. Boyce - One of the best experts on this subject based on the ideXlab platform.
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Screening of 71 P. multocida proteins for protective efficacy in a Fowl Cholera infection model and characterization of the protective antigen PlpE
PloS one, 2012Co-Authors: Tamas Zsolt Hatfaludi, Keith Al-hasani, Lan Gong, John D. Boyce, Mark Ford, Ian W Wilkie, Noelene Sheila Quinsey, Michelle A. Dunstone, David E. Hoke, Ben AdlerAbstract:Background: There is a strong need for a recombinant subunit vaccine against Fowl Cholera. We used a reverse vaccinology approach to identify putative secreted or cell surface associated P. multocida proteins that may represent potential vaccine candidate antigens. Principal Findings: A high-throughput cloning and expression protocol was used to express and purify 71 recombinant proteins for vaccine trials. Of the 71 proteins tested, only one, PlpE in denatured insoluble form, protected chickens against Fowl Cholera challenge. PlpE also elicited comparable levels of protection in mice. PlpE was localized by immunofluorescence to the bacterial cell surface, consistent with its ability to elicit a protective immune response. To explore the role of PlpE during infection and immunity, a plpE mutant was generated. The plpE mutant strain retained full virulence for mice. Conclusion: These studies show that PlpE is a surface exposed protein and was the only protein of 71 tested that was able to elicit a protective immune response. However, PlpE is not an essential virulence factor. This is the first report of a denatured recombinant protein stimulating protection against Fowl Cholera.
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Vaccination against Fowl Cholera with acapsular Pasteurella multocida A:1.
Vaccine, 2005Co-Authors: Jing Yeng Chung, John D. Boyce, Ian W Wilkie, Ben AdlerAbstract:We have previously constructed an acapsular Pasteurella multocida X-73 (serogroup A) mutant strain which was attenuated in virulence for chickens (Chung JY, Wilkie IW, Boyce JD, Townsend KM, Frost AJ, Ghodussi M, Adler B. Role of capsule in the pathogenesis of Fowl Cholera caused by Pasteurella multocida serogroup A. Infect. Immun. 2001;69:2487-2492). In this study, we have assessed the ability of this acapsular strain (PBA930) to induce protection against wild-type challenge in mice and the natural host chickens. Intramuscular administration of PBA930 to mice stimulated significant protection against X-73 and the heterologous strain P-1059 (A:3), but not against challenge with P-1662 (A:4). No protection was observed when PBA930 was introduced by the intraperitoneal or subcutaneous routes in mice. Significantly, the acapsular strain PBA930 was able to induce protection against challenge with wild type X-73 in chickens.
Tamas Zsolt Hatfaludi - One of the best experts on this subject based on the ideXlab platform.
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Screening of 71 P. multocida proteins for protective efficacy in a Fowl Cholera infection model and characterization of the protective antigen PlpE
PloS one, 2012Co-Authors: Tamas Zsolt Hatfaludi, Keith Al-hasani, Lan Gong, John D. Boyce, Mark Ford, Ian W Wilkie, Noelene Sheila Quinsey, Michelle A. Dunstone, David E. Hoke, Ben AdlerAbstract:Background: There is a strong need for a recombinant subunit vaccine against Fowl Cholera. We used a reverse vaccinology approach to identify putative secreted or cell surface associated P. multocida proteins that may represent potential vaccine candidate antigens. Principal Findings: A high-throughput cloning and expression protocol was used to express and purify 71 recombinant proteins for vaccine trials. Of the 71 proteins tested, only one, PlpE in denatured insoluble form, protected chickens against Fowl Cholera challenge. PlpE also elicited comparable levels of protection in mice. PlpE was localized by immunofluorescence to the bacterial cell surface, consistent with its ability to elicit a protective immune response. To explore the role of PlpE during infection and immunity, a plpE mutant was generated. The plpE mutant strain retained full virulence for mice. Conclusion: These studies show that PlpE is a surface exposed protein and was the only protein of 71 tested that was able to elicit a protective immune response. However, PlpE is not an essential virulence factor. This is the first report of a denatured recombinant protein stimulating protection against Fowl Cholera.
David E. Hoke - One of the best experts on this subject based on the ideXlab platform.
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Screening of 71 P. multocida proteins for protective efficacy in a Fowl Cholera infection model and characterization of the protective antigen PlpE
PloS one, 2012Co-Authors: Tamas Zsolt Hatfaludi, Keith Al-hasani, Lan Gong, John D. Boyce, Mark Ford, Ian W Wilkie, Noelene Sheila Quinsey, Michelle A. Dunstone, David E. Hoke, Ben AdlerAbstract:Background: There is a strong need for a recombinant subunit vaccine against Fowl Cholera. We used a reverse vaccinology approach to identify putative secreted or cell surface associated P. multocida proteins that may represent potential vaccine candidate antigens. Principal Findings: A high-throughput cloning and expression protocol was used to express and purify 71 recombinant proteins for vaccine trials. Of the 71 proteins tested, only one, PlpE in denatured insoluble form, protected chickens against Fowl Cholera challenge. PlpE also elicited comparable levels of protection in mice. PlpE was localized by immunofluorescence to the bacterial cell surface, consistent with its ability to elicit a protective immune response. To explore the role of PlpE during infection and immunity, a plpE mutant was generated. The plpE mutant strain retained full virulence for mice. Conclusion: These studies show that PlpE is a surface exposed protein and was the only protein of 71 tested that was able to elicit a protective immune response. However, PlpE is not an essential virulence factor. This is the first report of a denatured recombinant protein stimulating protection against Fowl Cholera.
