The Experts below are selected from a list of 24 Experts worldwide ranked by ideXlab platform
Tsutomu Kouyama - One of the best experts on this subject based on the ideXlab platform.
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direct observations of Freeze Etching processes of ice embedded biomembranes by atomic force microscopy
Micron, 2003Co-Authors: Daisuke Yamamoto, Koji Tani, Toshiaki Gotoh, Tsutomu KouyamaAbstract:We have fabricated a cryogenic atomic force microscope that is designed for structural investigation of Freeze-fractured biological specimens. The apparatus is operated in liquid nitrogen gas at atmospheric pressure. Freeze-fracturing, Freeze-Etching and subsequent imaging are carried out in the same chamber, so that the surface topography of a fractured plane is easily visualized without ice contamination. A controlled superficial sublimation of volatile molecules allows us to obtain three-dimensional views of ultrastructures of biological membranes.
Daisuke Yamamoto - One of the best experts on this subject based on the ideXlab platform.
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direct observations of Freeze Etching processes of ice embedded biomembranes by atomic force microscopy
Micron, 2003Co-Authors: Daisuke Yamamoto, Koji Tani, Toshiaki Gotoh, Tsutomu KouyamaAbstract:We have fabricated a cryogenic atomic force microscope that is designed for structural investigation of Freeze-fractured biological specimens. The apparatus is operated in liquid nitrogen gas at atmospheric pressure. Freeze-fracturing, Freeze-Etching and subsequent imaging are carried out in the same chamber, so that the surface topography of a fractured plane is easily visualized without ice contamination. A controlled superficial sublimation of volatile molecules allows us to obtain three-dimensional views of ultrastructures of biological membranes.
Toshiaki Gotoh - One of the best experts on this subject based on the ideXlab platform.
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direct observations of Freeze Etching processes of ice embedded biomembranes by atomic force microscopy
Micron, 2003Co-Authors: Daisuke Yamamoto, Koji Tani, Toshiaki Gotoh, Tsutomu KouyamaAbstract:We have fabricated a cryogenic atomic force microscope that is designed for structural investigation of Freeze-fractured biological specimens. The apparatus is operated in liquid nitrogen gas at atmospheric pressure. Freeze-fracturing, Freeze-Etching and subsequent imaging are carried out in the same chamber, so that the surface topography of a fractured plane is easily visualized without ice contamination. A controlled superficial sublimation of volatile molecules allows us to obtain three-dimensional views of ultrastructures of biological membranes.
Koji Tani - One of the best experts on this subject based on the ideXlab platform.
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direct observations of Freeze Etching processes of ice embedded biomembranes by atomic force microscopy
Micron, 2003Co-Authors: Daisuke Yamamoto, Koji Tani, Toshiaki Gotoh, Tsutomu KouyamaAbstract:We have fabricated a cryogenic atomic force microscope that is designed for structural investigation of Freeze-fractured biological specimens. The apparatus is operated in liquid nitrogen gas at atmospheric pressure. Freeze-fracturing, Freeze-Etching and subsequent imaging are carried out in the same chamber, so that the surface topography of a fractured plane is easily visualized without ice contamination. A controlled superficial sublimation of volatile molecules allows us to obtain three-dimensional views of ultrastructures of biological membranes.
Zhang Yun-huai - One of the best experts on this subject based on the ideXlab platform.
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In-situ Synthesis of a Non-aqueous Nano-dispersion System by the Modified Freeze-Etching Method and Its Characterization by Freeze-Etching Replication Transmission Electron Microscopy
Journal of Instrumental Analysis, 2006Co-Authors: Xiao Peng, Zhang Yun-huaiAbstract:A method for the in-situ synthesis of non-aqueous nano-dispersion system for specimen preparation using the modified Freeze-Etching method was reported.The particle size,the particle size distribution and aggregation state in the nano-dispersion phase were then characterized by the Freeze-Etching replication transmission electron microscopy(FERTEM).The results showed that the particle size and the particle size distribution characterized by FERTEM were the same as those characterized by the laser scattering method,but the former was more accurate,visual,distinct and steric,and could collect multi-information simultaneously.FERTEM is an ideal method for characterizing the non-aqueous nano-dispersion system.
