The Experts below are selected from a list of 4395 Experts worldwide ranked by ideXlab platform
Zhihui Cheng - One of the best experts on this subject based on the ideXlab platform.
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map based cloning identification and characterization of the w gene controlling white immature Fruit Color in cucumber cucumis sativus l
Theoretical and Applied Genetics, 2016Co-Authors: Jianqing Jiao, Xinjing Liang, Huanwen Meng, Shuxia Chen, Yuhong Li, Zhihui ChengAbstract:Key message A single-nucleotide insertion resulted in a premature stop codon that is responsible for white immature Fruit Color in cucumber.
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Map-based cloning, identification and characterization of the w gene controlling white immature Fruit Color in cucumber (Cucumis sativus L.)
Theoretical and Applied Genetics, 2016Co-Authors: Hanqiang Liu, Jianqing Jiao, Xinjing Liang, Huanwen Meng, Shuxia Chen, Yuhong Li, Jia Liu, Zhihui ChengAbstract:KEY MESSAGE: A single-nucleotide insertion resulted in a premature stop codon that is responsible for white immature Fruit Color in cucumber. Despite our previous progress in the mapping of the gene controlling white Color in immature cucumber Fruit and the identification of candidate genes, the specific gene that governs chlorophyll metabolism and its regulatory mechanism remains unknown. Here, we generated a mapping population consisting of 9497 F2 plants to delimit the controlling gene to an 8.2-kb physical interval that defines a sole candidate gene, APRR2. Sequencing the full-length DNA and cDNA of APRR2 allowed for identification of an allele, aprr2, encoding a truncated 101-amino acid protein due to a frameshift mutation and a premature stop codon. Gene structure prediction indicated that these 101 residues are located in a domain necessary for the function of the protein. The expression patterns of APRR2 were entirely consistent with the visual changes in green Color intensity during Fruit development. A microscopic observation of the Fruit pericarp revealed fewer chloroplasts and a lower chloroplast chlorophyll storage capacity in Q24 (white) than in Q30 (green). A single-base insertion in the white Color gene w, which leads to a premature stop codon, is hypothesized to have disabled the function of this gene in chlorophyll accumulation and chloroplast development. These findings contribute to basic research and the genetic improvement of Fruit Color.
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fine genetic mapping of the white immature Fruit Color gene w to a 33 0 kb region in cucumber cucumis sativus l
Theoretical and Applied Genetics, 2015Co-Authors: Huanwen Meng, Jianqing Jiao, Xinjing Liang, Shuxia Chen, Yuhong Li, Zhihui ChengAbstract:Key message The white immature Fruit Color genewwas rapidly mapped to a 33.0-kb region to identify a valuable candidate gene that encodes peroxidase.
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Fine genetic mapping of the white immature Fruit Color gene w to a 33.0-kb region in cucumber (Cucumis sativus L.)
Theoretical and Applied Genetics, 2015Co-Authors: Hanqiang Liu, Jianqing Jiao, Xinjing Liang, Huanwen Meng, Shuxia Chen, Yupeng Pan, Yuhong Li, Zhihui ChengAbstract:KEY MESSAGE: The white immature Fruit Color gene w was rapidly mapped to a 33.0-kb region to identify a valuable candidate gene that encodes peroxidase. The skin Color of immature Fruit is a crucial external trait of cucumbers, and white skin is shared by limited numbers of commercial cultivars. Herein, one BC1 population and two F2 segregating populations were constructed using four inbred parental lines (WD3 × B-2-2 and Q30 × Q24) to investigate the inheritance patterns and chromosomal locations of immature Fruit Color genes in cucumbers. Consequently, a single recessive gene, w, was identified that controls white immature Fruit Color. A total of 526 markers, which were derived from published genetic maps, two reference cucumber genomes ("9930" and GY14), and two parents (Q30 and Q24) for which whole-genome sequence information is available, were used to map the target gene w to a 33.0-kb region flanked by two SNP-based markers, ASPCR39262 and ASPCR39229, which are physically located at 39262450 and 39229482 of chromosome 3 ("9930" draft genome assembly), respectively. Gene prediction indicated that four potential genes were located in the target region. One gene that encodes peroxidase is likely to be a valuable candidate gene because quantitative real-time PCR revealed an eightfold difference in its transcriptional level, and several amino acid variations were found when the deduced amino acid sequence was aligned. A co-segregating marker was used synergistically to test its ability to predict the skin Colors of 83 dark green/white germplasms, and the validity of its utility in marker-assisted selection was confirmed. Fine mapping of this locus will assist in cloning the gene and in marker-assisted breeding to develop dark green/white cucumber cultivars.
Jianqing Jiao - One of the best experts on this subject based on the ideXlab platform.
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map based cloning identification and characterization of the w gene controlling white immature Fruit Color in cucumber cucumis sativus l
Theoretical and Applied Genetics, 2016Co-Authors: Jianqing Jiao, Xinjing Liang, Huanwen Meng, Shuxia Chen, Yuhong Li, Zhihui ChengAbstract:Key message A single-nucleotide insertion resulted in a premature stop codon that is responsible for white immature Fruit Color in cucumber.
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Map-based cloning, identification and characterization of the w gene controlling white immature Fruit Color in cucumber (Cucumis sativus L.)
Theoretical and Applied Genetics, 2016Co-Authors: Hanqiang Liu, Jianqing Jiao, Xinjing Liang, Huanwen Meng, Shuxia Chen, Yuhong Li, Jia Liu, Zhihui ChengAbstract:KEY MESSAGE: A single-nucleotide insertion resulted in a premature stop codon that is responsible for white immature Fruit Color in cucumber. Despite our previous progress in the mapping of the gene controlling white Color in immature cucumber Fruit and the identification of candidate genes, the specific gene that governs chlorophyll metabolism and its regulatory mechanism remains unknown. Here, we generated a mapping population consisting of 9497 F2 plants to delimit the controlling gene to an 8.2-kb physical interval that defines a sole candidate gene, APRR2. Sequencing the full-length DNA and cDNA of APRR2 allowed for identification of an allele, aprr2, encoding a truncated 101-amino acid protein due to a frameshift mutation and a premature stop codon. Gene structure prediction indicated that these 101 residues are located in a domain necessary for the function of the protein. The expression patterns of APRR2 were entirely consistent with the visual changes in green Color intensity during Fruit development. A microscopic observation of the Fruit pericarp revealed fewer chloroplasts and a lower chloroplast chlorophyll storage capacity in Q24 (white) than in Q30 (green). A single-base insertion in the white Color gene w, which leads to a premature stop codon, is hypothesized to have disabled the function of this gene in chlorophyll accumulation and chloroplast development. These findings contribute to basic research and the genetic improvement of Fruit Color.
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fine genetic mapping of the white immature Fruit Color gene w to a 33 0 kb region in cucumber cucumis sativus l
Theoretical and Applied Genetics, 2015Co-Authors: Huanwen Meng, Jianqing Jiao, Xinjing Liang, Shuxia Chen, Yuhong Li, Zhihui ChengAbstract:Key message The white immature Fruit Color genewwas rapidly mapped to a 33.0-kb region to identify a valuable candidate gene that encodes peroxidase.
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Fine genetic mapping of the white immature Fruit Color gene w to a 33.0-kb region in cucumber (Cucumis sativus L.)
Theoretical and Applied Genetics, 2015Co-Authors: Hanqiang Liu, Jianqing Jiao, Xinjing Liang, Huanwen Meng, Shuxia Chen, Yupeng Pan, Yuhong Li, Zhihui ChengAbstract:KEY MESSAGE: The white immature Fruit Color gene w was rapidly mapped to a 33.0-kb region to identify a valuable candidate gene that encodes peroxidase. The skin Color of immature Fruit is a crucial external trait of cucumbers, and white skin is shared by limited numbers of commercial cultivars. Herein, one BC1 population and two F2 segregating populations were constructed using four inbred parental lines (WD3 × B-2-2 and Q30 × Q24) to investigate the inheritance patterns and chromosomal locations of immature Fruit Color genes in cucumbers. Consequently, a single recessive gene, w, was identified that controls white immature Fruit Color. A total of 526 markers, which were derived from published genetic maps, two reference cucumber genomes ("9930" and GY14), and two parents (Q30 and Q24) for which whole-genome sequence information is available, were used to map the target gene w to a 33.0-kb region flanked by two SNP-based markers, ASPCR39262 and ASPCR39229, which are physically located at 39262450 and 39229482 of chromosome 3 ("9930" draft genome assembly), respectively. Gene prediction indicated that four potential genes were located in the target region. One gene that encodes peroxidase is likely to be a valuable candidate gene because quantitative real-time PCR revealed an eightfold difference in its transcriptional level, and several amino acid variations were found when the deduced amino acid sequence was aligned. A co-segregating marker was used synergistically to test its ability to predict the skin Colors of 83 dark green/white germplasms, and the validity of its utility in marker-assisted selection was confirmed. Fine mapping of this locus will assist in cloning the gene and in marker-assisted breeding to develop dark green/white cucumber cultivars.
Huanwen Meng - One of the best experts on this subject based on the ideXlab platform.
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map based cloning identification and characterization of the w gene controlling white immature Fruit Color in cucumber cucumis sativus l
Theoretical and Applied Genetics, 2016Co-Authors: Jianqing Jiao, Xinjing Liang, Huanwen Meng, Shuxia Chen, Yuhong Li, Zhihui ChengAbstract:Key message A single-nucleotide insertion resulted in a premature stop codon that is responsible for white immature Fruit Color in cucumber.
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Map-based cloning, identification and characterization of the w gene controlling white immature Fruit Color in cucumber (Cucumis sativus L.)
Theoretical and Applied Genetics, 2016Co-Authors: Hanqiang Liu, Jianqing Jiao, Xinjing Liang, Huanwen Meng, Shuxia Chen, Yuhong Li, Jia Liu, Zhihui ChengAbstract:KEY MESSAGE: A single-nucleotide insertion resulted in a premature stop codon that is responsible for white immature Fruit Color in cucumber. Despite our previous progress in the mapping of the gene controlling white Color in immature cucumber Fruit and the identification of candidate genes, the specific gene that governs chlorophyll metabolism and its regulatory mechanism remains unknown. Here, we generated a mapping population consisting of 9497 F2 plants to delimit the controlling gene to an 8.2-kb physical interval that defines a sole candidate gene, APRR2. Sequencing the full-length DNA and cDNA of APRR2 allowed for identification of an allele, aprr2, encoding a truncated 101-amino acid protein due to a frameshift mutation and a premature stop codon. Gene structure prediction indicated that these 101 residues are located in a domain necessary for the function of the protein. The expression patterns of APRR2 were entirely consistent with the visual changes in green Color intensity during Fruit development. A microscopic observation of the Fruit pericarp revealed fewer chloroplasts and a lower chloroplast chlorophyll storage capacity in Q24 (white) than in Q30 (green). A single-base insertion in the white Color gene w, which leads to a premature stop codon, is hypothesized to have disabled the function of this gene in chlorophyll accumulation and chloroplast development. These findings contribute to basic research and the genetic improvement of Fruit Color.
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fine genetic mapping of the white immature Fruit Color gene w to a 33 0 kb region in cucumber cucumis sativus l
Theoretical and Applied Genetics, 2015Co-Authors: Huanwen Meng, Jianqing Jiao, Xinjing Liang, Shuxia Chen, Yuhong Li, Zhihui ChengAbstract:Key message The white immature Fruit Color genewwas rapidly mapped to a 33.0-kb region to identify a valuable candidate gene that encodes peroxidase.
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Fine genetic mapping of the white immature Fruit Color gene w to a 33.0-kb region in cucumber (Cucumis sativus L.)
Theoretical and Applied Genetics, 2015Co-Authors: Hanqiang Liu, Jianqing Jiao, Xinjing Liang, Huanwen Meng, Shuxia Chen, Yupeng Pan, Yuhong Li, Zhihui ChengAbstract:KEY MESSAGE: The white immature Fruit Color gene w was rapidly mapped to a 33.0-kb region to identify a valuable candidate gene that encodes peroxidase. The skin Color of immature Fruit is a crucial external trait of cucumbers, and white skin is shared by limited numbers of commercial cultivars. Herein, one BC1 population and two F2 segregating populations were constructed using four inbred parental lines (WD3 × B-2-2 and Q30 × Q24) to investigate the inheritance patterns and chromosomal locations of immature Fruit Color genes in cucumbers. Consequently, a single recessive gene, w, was identified that controls white immature Fruit Color. A total of 526 markers, which were derived from published genetic maps, two reference cucumber genomes ("9930" and GY14), and two parents (Q30 and Q24) for which whole-genome sequence information is available, were used to map the target gene w to a 33.0-kb region flanked by two SNP-based markers, ASPCR39262 and ASPCR39229, which are physically located at 39262450 and 39229482 of chromosome 3 ("9930" draft genome assembly), respectively. Gene prediction indicated that four potential genes were located in the target region. One gene that encodes peroxidase is likely to be a valuable candidate gene because quantitative real-time PCR revealed an eightfold difference in its transcriptional level, and several amino acid variations were found when the deduced amino acid sequence was aligned. A co-segregating marker was used synergistically to test its ability to predict the skin Colors of 83 dark green/white germplasms, and the validity of its utility in marker-assisted selection was confirmed. Fine mapping of this locus will assist in cloning the gene and in marker-assisted breeding to develop dark green/white cucumber cultivars.
Yuhong Li - One of the best experts on this subject based on the ideXlab platform.
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map based cloning identification and characterization of the w gene controlling white immature Fruit Color in cucumber cucumis sativus l
Theoretical and Applied Genetics, 2016Co-Authors: Jianqing Jiao, Xinjing Liang, Huanwen Meng, Shuxia Chen, Yuhong Li, Zhihui ChengAbstract:Key message A single-nucleotide insertion resulted in a premature stop codon that is responsible for white immature Fruit Color in cucumber.
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Map-based cloning, identification and characterization of the w gene controlling white immature Fruit Color in cucumber (Cucumis sativus L.)
Theoretical and Applied Genetics, 2016Co-Authors: Hanqiang Liu, Jianqing Jiao, Xinjing Liang, Huanwen Meng, Shuxia Chen, Yuhong Li, Jia Liu, Zhihui ChengAbstract:KEY MESSAGE: A single-nucleotide insertion resulted in a premature stop codon that is responsible for white immature Fruit Color in cucumber. Despite our previous progress in the mapping of the gene controlling white Color in immature cucumber Fruit and the identification of candidate genes, the specific gene that governs chlorophyll metabolism and its regulatory mechanism remains unknown. Here, we generated a mapping population consisting of 9497 F2 plants to delimit the controlling gene to an 8.2-kb physical interval that defines a sole candidate gene, APRR2. Sequencing the full-length DNA and cDNA of APRR2 allowed for identification of an allele, aprr2, encoding a truncated 101-amino acid protein due to a frameshift mutation and a premature stop codon. Gene structure prediction indicated that these 101 residues are located in a domain necessary for the function of the protein. The expression patterns of APRR2 were entirely consistent with the visual changes in green Color intensity during Fruit development. A microscopic observation of the Fruit pericarp revealed fewer chloroplasts and a lower chloroplast chlorophyll storage capacity in Q24 (white) than in Q30 (green). A single-base insertion in the white Color gene w, which leads to a premature stop codon, is hypothesized to have disabled the function of this gene in chlorophyll accumulation and chloroplast development. These findings contribute to basic research and the genetic improvement of Fruit Color.
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fine genetic mapping of the white immature Fruit Color gene w to a 33 0 kb region in cucumber cucumis sativus l
Theoretical and Applied Genetics, 2015Co-Authors: Huanwen Meng, Jianqing Jiao, Xinjing Liang, Shuxia Chen, Yuhong Li, Zhihui ChengAbstract:Key message The white immature Fruit Color genewwas rapidly mapped to a 33.0-kb region to identify a valuable candidate gene that encodes peroxidase.
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Fine genetic mapping of the white immature Fruit Color gene w to a 33.0-kb region in cucumber (Cucumis sativus L.)
Theoretical and Applied Genetics, 2015Co-Authors: Hanqiang Liu, Jianqing Jiao, Xinjing Liang, Huanwen Meng, Shuxia Chen, Yupeng Pan, Yuhong Li, Zhihui ChengAbstract:KEY MESSAGE: The white immature Fruit Color gene w was rapidly mapped to a 33.0-kb region to identify a valuable candidate gene that encodes peroxidase. The skin Color of immature Fruit is a crucial external trait of cucumbers, and white skin is shared by limited numbers of commercial cultivars. Herein, one BC1 population and two F2 segregating populations were constructed using four inbred parental lines (WD3 × B-2-2 and Q30 × Q24) to investigate the inheritance patterns and chromosomal locations of immature Fruit Color genes in cucumbers. Consequently, a single recessive gene, w, was identified that controls white immature Fruit Color. A total of 526 markers, which were derived from published genetic maps, two reference cucumber genomes ("9930" and GY14), and two parents (Q30 and Q24) for which whole-genome sequence information is available, were used to map the target gene w to a 33.0-kb region flanked by two SNP-based markers, ASPCR39262 and ASPCR39229, which are physically located at 39262450 and 39229482 of chromosome 3 ("9930" draft genome assembly), respectively. Gene prediction indicated that four potential genes were located in the target region. One gene that encodes peroxidase is likely to be a valuable candidate gene because quantitative real-time PCR revealed an eightfold difference in its transcriptional level, and several amino acid variations were found when the deduced amino acid sequence was aligned. A co-segregating marker was used synergistically to test its ability to predict the skin Colors of 83 dark green/white germplasms, and the validity of its utility in marker-assisted selection was confirmed. Fine mapping of this locus will assist in cloning the gene and in marker-assisted breeding to develop dark green/white cucumber cultivars.
Xinjing Liang - One of the best experts on this subject based on the ideXlab platform.
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map based cloning identification and characterization of the w gene controlling white immature Fruit Color in cucumber cucumis sativus l
Theoretical and Applied Genetics, 2016Co-Authors: Jianqing Jiao, Xinjing Liang, Huanwen Meng, Shuxia Chen, Yuhong Li, Zhihui ChengAbstract:Key message A single-nucleotide insertion resulted in a premature stop codon that is responsible for white immature Fruit Color in cucumber.
-
Map-based cloning, identification and characterization of the w gene controlling white immature Fruit Color in cucumber (Cucumis sativus L.)
Theoretical and Applied Genetics, 2016Co-Authors: Hanqiang Liu, Jianqing Jiao, Xinjing Liang, Huanwen Meng, Shuxia Chen, Yuhong Li, Jia Liu, Zhihui ChengAbstract:KEY MESSAGE: A single-nucleotide insertion resulted in a premature stop codon that is responsible for white immature Fruit Color in cucumber. Despite our previous progress in the mapping of the gene controlling white Color in immature cucumber Fruit and the identification of candidate genes, the specific gene that governs chlorophyll metabolism and its regulatory mechanism remains unknown. Here, we generated a mapping population consisting of 9497 F2 plants to delimit the controlling gene to an 8.2-kb physical interval that defines a sole candidate gene, APRR2. Sequencing the full-length DNA and cDNA of APRR2 allowed for identification of an allele, aprr2, encoding a truncated 101-amino acid protein due to a frameshift mutation and a premature stop codon. Gene structure prediction indicated that these 101 residues are located in a domain necessary for the function of the protein. The expression patterns of APRR2 were entirely consistent with the visual changes in green Color intensity during Fruit development. A microscopic observation of the Fruit pericarp revealed fewer chloroplasts and a lower chloroplast chlorophyll storage capacity in Q24 (white) than in Q30 (green). A single-base insertion in the white Color gene w, which leads to a premature stop codon, is hypothesized to have disabled the function of this gene in chlorophyll accumulation and chloroplast development. These findings contribute to basic research and the genetic improvement of Fruit Color.
-
fine genetic mapping of the white immature Fruit Color gene w to a 33 0 kb region in cucumber cucumis sativus l
Theoretical and Applied Genetics, 2015Co-Authors: Huanwen Meng, Jianqing Jiao, Xinjing Liang, Shuxia Chen, Yuhong Li, Zhihui ChengAbstract:Key message The white immature Fruit Color genewwas rapidly mapped to a 33.0-kb region to identify a valuable candidate gene that encodes peroxidase.
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Fine genetic mapping of the white immature Fruit Color gene w to a 33.0-kb region in cucumber (Cucumis sativus L.)
Theoretical and Applied Genetics, 2015Co-Authors: Hanqiang Liu, Jianqing Jiao, Xinjing Liang, Huanwen Meng, Shuxia Chen, Yupeng Pan, Yuhong Li, Zhihui ChengAbstract:KEY MESSAGE: The white immature Fruit Color gene w was rapidly mapped to a 33.0-kb region to identify a valuable candidate gene that encodes peroxidase. The skin Color of immature Fruit is a crucial external trait of cucumbers, and white skin is shared by limited numbers of commercial cultivars. Herein, one BC1 population and two F2 segregating populations were constructed using four inbred parental lines (WD3 × B-2-2 and Q30 × Q24) to investigate the inheritance patterns and chromosomal locations of immature Fruit Color genes in cucumbers. Consequently, a single recessive gene, w, was identified that controls white immature Fruit Color. A total of 526 markers, which were derived from published genetic maps, two reference cucumber genomes ("9930" and GY14), and two parents (Q30 and Q24) for which whole-genome sequence information is available, were used to map the target gene w to a 33.0-kb region flanked by two SNP-based markers, ASPCR39262 and ASPCR39229, which are physically located at 39262450 and 39229482 of chromosome 3 ("9930" draft genome assembly), respectively. Gene prediction indicated that four potential genes were located in the target region. One gene that encodes peroxidase is likely to be a valuable candidate gene because quantitative real-time PCR revealed an eightfold difference in its transcriptional level, and several amino acid variations were found when the deduced amino acid sequence was aligned. A co-segregating marker was used synergistically to test its ability to predict the skin Colors of 83 dark green/white germplasms, and the validity of its utility in marker-assisted selection was confirmed. Fine mapping of this locus will assist in cloning the gene and in marker-assisted breeding to develop dark green/white cucumber cultivars.
