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Susana M. Cardoso - One of the best experts on this subject based on the ideXlab platform.
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Impact of Phlorotannin Extracts from Fucus vesiculosus on Human Gut Microbiota
MDPI AG, 2021Co-Authors: Marcelo D. Catarino, Catarina Marçal, Artur M. S. Silva, Teresa Bonifácio-lopes, Débora Campos, Nuno Mateus, Maria Manuela Pintado, Susana M. CardosoAbstract:Recent studies indicate that plant polyphenols could be pointed as potential prebiotic candidates since they may interact with the gut microbiota, stimulating its growth and the production of metabolites. However, little is known about the fate of brown seaweeds’ phlorotannins during their passage throughout the gastrointestinal tract. This work aimed to evaluate the stability and bioaccessibility of Fucus vesiculosus phlorotannins after being submitted to a simulated digestive process, as well as their possible modulatory effects on gut microbiota and short-chain fatty acids production following a fermentation procedure using fecal inoculates to mimic the conditions of the large intestine. The stability of phlorotannins throughout the gastrointestinal tract was reduced, with a bioaccessibility index between 2 and 14%. Moreover, slight alterations in the growth of certain commensal bacteria were noticed, with Enterococcus spp. being the most enhanced group. Likewise, F. vesiculosus phlorotannins displayed striking capacity to enhance the levels of propionate and butyrate, which are two important short-chain fatty acids known for their role in intestinal homeostasis. In summary, this work provides valuable information regarding the behavior of F. vesiculosus phlorotannins along the gastrointestinal tract, presenting clear evidence that these compounds can positively contribute to the maintenance of a healthy gastrointestinal condition.
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Microwave-Assisted Extraction of Phlorotannins from Fucus vesiculosus.
Marine drugs, 2020Co-Authors: Sónia J Amarante, Catarina Marçal, Artur M. S. Silva, Marcelo D. Catarino, Rita Ferreira, Susana M. CardosoAbstract:Microwave-assisted extraction (MAE) was carried out to maximize the extraction of phlorotannins from Fucus vesiculosus using a hydroethanolic mixture as a solvent, as an alternative to the conventional method with a hydroacetonic mixture. Optimal MAE conditions were set as ethanol concentration of 57% (v/v), temperature of 75 °C, and time of 5 min, which allowed a similar recovery of phlorotannins from the macroalgae compared to the conventional extraction. While the phlorotannins richness of the conventional extract was slightly superior to that of MAE (11.1 ± 1.3 vs. 9.8 ± 1.8 mg PGE/g DWextract), both extracts presented identical phlorotannins constituents, which included, among others, tetrafucol, pentafucol, hexafucol, and heptafucol structures. In addition, MAE showed a moderate capacity to scavenge ABTS•+ (IC50 of 96.0 ± 3.4 µg/mL) and to inhibit the activity of xanthine oxidase (IC50 of 23.1 ± 3.4 µg/mL) and a superior ability to control the activity of the key metabolic enzyme α-glucosidase compared to the pharmaceutical drug acarbose.
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Effect of Oven-Drying on the Recovery of Valuable Compounds from Ulva rigida, Gracilaria sp. and Fucus vesiculosus
MDPI AG, 2019Co-Authors: Andreia F.r. Silva, Helena Abreu, Artur M. S. Silva, Susana M. CardosoAbstract:The effect of oven-drying at 25, 40 and 60 °C was evaluated on three macroalgae of relevance in Europe, namely Ulva rigida, Gracilaria sp. and Fucus vesiculosus, with respect to quality aspects, including their potential to be exploited as a source of valuable compounds. Notably, as compared to freeze-drying, oven-drying at 25 °C promoted the extraction of chlorophylls and carotenoids from U. rigida, as well as those of phycoerythrin and chlorophyll a from Gracilaria sp., while 40 °C favored the recovery of fucoxanthin and pheophytin a from F. vesiculosus. On the other hand, the use of oven-drying had a negative impact on the extraction of phenolic compounds from this alga, also diminishing the antioxidant activity of the resulting extracts. Instead, the impact of oven-drying of raw material on the recovery of specific polysaccharides differed among the macroalgae. While the amounts of ulvans and fucoidans obtained from macroalgae dried at higher temperatures tended to be superior, the recovery of agar was not affected with the drying temperatures applied to Gracilaria sp. The overall results showed that oven-drying might serve as a good alternative to stabilize Ulva rigida, Gracilaria sp. and Fucus vesiculosus, especially if extraction of pigments and polysaccharides is aimed, thought the appropriate temperature applied must be adapted for each macroalgae
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Screening of Ulva rigida, Gracilaria sp., Fucus vesiculosus and Saccharina latissima as Functional Ingredients.
International journal of molecular sciences, 2018Co-Authors: Rodrigo T Neto, Catarina Marçal, Ana S Queirós, Helena Abreu, Artur M. S. Silva, Susana M. CardosoAbstract:The intent of the present work was to evaluate the potential of four macroalgae prevalent in Europe, namely Ulva rigida, Gracilaria sp., Fucus vesiculosus and Saccharina latissima, for application in functional foods, either in the direct form or as extracts. Accordingly, nutritional composition, the content of phytochemical antioxidants, and the inhibitory ability of key enzymes with impacts on obesity and diabetes (α-glucosidase and pancreatic lipase) or on arterial pressure (angiotensin-I converting enzyme), were evaluated. Overall, protein, lipid, ash and fiber contents of the macroalgae ranged from 9–24% dw, 0.5–3.0% dw, 20–32% dw, and 37–45% dw, respectively, making them good candidates for nutritional supplementation of several foods, particularly due to their mineral and fiber contents. In addition, brown macroalgae, in particular F. vesiculosus, stood out for its superior phenolic content, which was reflected by its high antioxidant ability and inhibition towards α-glucosidase activity (0.032 mg/mL of hydroacetonic extract inhibited 50% of the enzyme activity).
Artur M. S. Silva - One of the best experts on this subject based on the ideXlab platform.
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Impact of Phlorotannin Extracts from Fucus vesiculosus on Human Gut Microbiota
MDPI AG, 2021Co-Authors: Marcelo D. Catarino, Catarina Marçal, Artur M. S. Silva, Teresa Bonifácio-lopes, Débora Campos, Nuno Mateus, Maria Manuela Pintado, Susana M. CardosoAbstract:Recent studies indicate that plant polyphenols could be pointed as potential prebiotic candidates since they may interact with the gut microbiota, stimulating its growth and the production of metabolites. However, little is known about the fate of brown seaweeds’ phlorotannins during their passage throughout the gastrointestinal tract. This work aimed to evaluate the stability and bioaccessibility of Fucus vesiculosus phlorotannins after being submitted to a simulated digestive process, as well as their possible modulatory effects on gut microbiota and short-chain fatty acids production following a fermentation procedure using fecal inoculates to mimic the conditions of the large intestine. The stability of phlorotannins throughout the gastrointestinal tract was reduced, with a bioaccessibility index between 2 and 14%. Moreover, slight alterations in the growth of certain commensal bacteria were noticed, with Enterococcus spp. being the most enhanced group. Likewise, F. vesiculosus phlorotannins displayed striking capacity to enhance the levels of propionate and butyrate, which are two important short-chain fatty acids known for their role in intestinal homeostasis. In summary, this work provides valuable information regarding the behavior of F. vesiculosus phlorotannins along the gastrointestinal tract, presenting clear evidence that these compounds can positively contribute to the maintenance of a healthy gastrointestinal condition.
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Microwave-Assisted Extraction of Phlorotannins from Fucus vesiculosus.
Marine drugs, 2020Co-Authors: Sónia J Amarante, Catarina Marçal, Artur M. S. Silva, Marcelo D. Catarino, Rita Ferreira, Susana M. CardosoAbstract:Microwave-assisted extraction (MAE) was carried out to maximize the extraction of phlorotannins from Fucus vesiculosus using a hydroethanolic mixture as a solvent, as an alternative to the conventional method with a hydroacetonic mixture. Optimal MAE conditions were set as ethanol concentration of 57% (v/v), temperature of 75 °C, and time of 5 min, which allowed a similar recovery of phlorotannins from the macroalgae compared to the conventional extraction. While the phlorotannins richness of the conventional extract was slightly superior to that of MAE (11.1 ± 1.3 vs. 9.8 ± 1.8 mg PGE/g DWextract), both extracts presented identical phlorotannins constituents, which included, among others, tetrafucol, pentafucol, hexafucol, and heptafucol structures. In addition, MAE showed a moderate capacity to scavenge ABTS•+ (IC50 of 96.0 ± 3.4 µg/mL) and to inhibit the activity of xanthine oxidase (IC50 of 23.1 ± 3.4 µg/mL) and a superior ability to control the activity of the key metabolic enzyme α-glucosidase compared to the pharmaceutical drug acarbose.
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Effect of Oven-Drying on the Recovery of Valuable Compounds from Ulva rigida, Gracilaria sp. and Fucus vesiculosus
MDPI AG, 2019Co-Authors: Andreia F.r. Silva, Helena Abreu, Artur M. S. Silva, Susana M. CardosoAbstract:The effect of oven-drying at 25, 40 and 60 °C was evaluated on three macroalgae of relevance in Europe, namely Ulva rigida, Gracilaria sp. and Fucus vesiculosus, with respect to quality aspects, including their potential to be exploited as a source of valuable compounds. Notably, as compared to freeze-drying, oven-drying at 25 °C promoted the extraction of chlorophylls and carotenoids from U. rigida, as well as those of phycoerythrin and chlorophyll a from Gracilaria sp., while 40 °C favored the recovery of fucoxanthin and pheophytin a from F. vesiculosus. On the other hand, the use of oven-drying had a negative impact on the extraction of phenolic compounds from this alga, also diminishing the antioxidant activity of the resulting extracts. Instead, the impact of oven-drying of raw material on the recovery of specific polysaccharides differed among the macroalgae. While the amounts of ulvans and fucoidans obtained from macroalgae dried at higher temperatures tended to be superior, the recovery of agar was not affected with the drying temperatures applied to Gracilaria sp. The overall results showed that oven-drying might serve as a good alternative to stabilize Ulva rigida, Gracilaria sp. and Fucus vesiculosus, especially if extraction of pigments and polysaccharides is aimed, thought the appropriate temperature applied must be adapted for each macroalgae
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Screening of Ulva rigida, Gracilaria sp., Fucus vesiculosus and Saccharina latissima as Functional Ingredients.
International journal of molecular sciences, 2018Co-Authors: Rodrigo T Neto, Catarina Marçal, Ana S Queirós, Helena Abreu, Artur M. S. Silva, Susana M. CardosoAbstract:The intent of the present work was to evaluate the potential of four macroalgae prevalent in Europe, namely Ulva rigida, Gracilaria sp., Fucus vesiculosus and Saccharina latissima, for application in functional foods, either in the direct form or as extracts. Accordingly, nutritional composition, the content of phytochemical antioxidants, and the inhibitory ability of key enzymes with impacts on obesity and diabetes (α-glucosidase and pancreatic lipase) or on arterial pressure (angiotensin-I converting enzyme), were evaluated. Overall, protein, lipid, ash and fiber contents of the macroalgae ranged from 9–24% dw, 0.5–3.0% dw, 20–32% dw, and 37–45% dw, respectively, making them good candidates for nutritional supplementation of several foods, particularly due to their mineral and fiber contents. In addition, brown macroalgae, in particular F. vesiculosus, stood out for its superior phenolic content, which was reflected by its high antioxidant ability and inhibition towards α-glucosidase activity (0.032 mg/mL of hydroacetonic extract inhibited 50% of the enzyme activity).
J A Munoz - One of the best experts on this subject based on the ideXlab platform.
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gold iii biosorption and bioreduction with the brown alga Fucus vesiculosus
Journal of Hazardous Materials, 2009Co-Authors: Y N Mata, Elena Torres, M L Blazquez, A Ballester, Felisa Gonzalez, J A MunozAbstract:Abstract In this paper, the bioreduction of Au(III) to Au(0) using biomass of the brown alga Fucus vesiculosus was investigated. The recovery and reduction process took place in two stages with an optimum pH range of 4–9 with a maximum uptake obtained at pH 7. In the first stage, an induction period previous to gold reduction, the variation of pH, redox potential and gold concentration in solution was practically negligible and no color change was observed. In the second stage, the gold reduction was followed by a sharp decrease of gold concentration, pH and redox potential of solution and a color change from yellow to reddish purple. Hydroxyl groups present in the algal polysaccharides were involved in the gold bioreduction. Metallic gold was detected as microprecipitates on the biomass surface and in colloidal form as nanoparticles in the solution. Bioreduction with F. vesiculosus could be an alternative and environmentally friendly process that can be used for recovering gold from dilute hydrometallurgical solutions and leachates of electronic scraps, and for the synthesis of gold nanoparticles of different size and shape.
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biosorption of cadmium lead and copper with calcium alginate xerogels and immobilized Fucus vesiculosus
Journal of Hazardous Materials, 2009Co-Authors: Y N Mata, M L Blazquez, A Ballester, F Gonzalez, J A MunozAbstract:Abstract This paper determines the effect of immobilized brown alga Fucus vesiculosus in the biosorption of heavy metals with alginate xerogels. Immobilization increased the kinetic uptakes and intraparticle diffusion rates of the three metals. The Langmuir maximum biosorption capacity increased twofold for cadmium, 10 times for lead, and decreased by half for copper. According to this model, the affinity of the metals for the biomass was as follows: Cu > Pb > Cd without alga and Pb > Cu > Cd with alga. FITR confirmed that carboxyl groups were the main groups involved in the metal uptake. Calcium in the gels was displaced by heavy metals from solution according to the “egg-box” model. The restructured gel matrix became more uniform and organized as shown by scanning electron microscopy (SEM) characterization. F. vesiculosus immobilized in alginate xerogels constitutes an excellent biosorbent for cadmium, lead and copper, sometimes surpassing the biosorption performance of alginate alone and even the free alga.
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characterization of the biosorption of cadmium lead and copper with the brown alga Fucus vesiculosus
Journal of Hazardous Materials, 2008Co-Authors: Y N Mata, M L Blazquez, A Ballester, F Gonzalez, J A MunozAbstract:The recovery of cadmium, lead and copper with the brown alga Fucus vesiculosus was characterized and quantified. The biosorption data fitted the pseudo-second order and Langmuir isotherm models, but did not adjust to the intraparticle diffusion model. The metal uptakes deduced from the pseudo-second order kinetic model and the Langmuir isotherm model followed a similar sequence: Cu > Cd ≈ Pb. The Langmuir maximum metal uptakes were: 0.9626 mmol/g, Pb 1.02 mmol/g, and Cu 1.66 mmol/g. According to the equilibrium constants of this isotherm model, the affinity of metals for the biomass followed this order: Pb > Cu > Cd. Biosorption was accomplished by ion exchange between metals in solution and algal protons, calcium and other light metals, and by complexation of the adsorbed metals with algal carboxyl groups. FTIR spectra showed a shift in the bands of carboxyl, hydroxyl and sulfonate groups. © 2008 Elsevier B.V. All rights reserved.
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biosorption of heavy metals by chemically activated alga Fucus vesiculosus
Journal of Chemical Technology & Biotechnology, 2005Co-Authors: J Rincon, M L Blazquez, A Ballester, F Gonzalez, J A MunozAbstract:The sorption capacity of the brown alga Fucus vesiculosus for copper, cadmium, lead and nickel was investigated. Metal sorption yields were modified using different kinds of pretreatment reagents: HCl, CaCl2, formaldehyde, Na2CO3 and NaOH. The Langmuir isotherm was applied to both the non-treated and all treated biomass tests. Calcium chloride was the only chemical that improved the maximum sorption capacity of the biomass. Copyright © 2005 Society of Chemical Industry
Antonio J Leal - One of the best experts on this subject based on the ideXlab platform.
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potential antioxidant capacity of sulfated polysaccharides from the edible marine brown seaweed Fucus vesiculosus
Journal of Agricultural and Food Chemistry, 2002Co-Authors: Pila Ruperez, Oussama Ahrazem, Antonio J LealAbstract:Fucus vesiculosus was sequentially extracted with water at 22 °C (fraction 1 (F1)) and 60 °C (F2), and with 0.1 M HCl (F3) and 2 M KOH (F4) at 37 °C. Soluble fractions (42.3% yield) were composed of neutral sugars (18.9−48 g/100 g), uronic acids (8.8−52.8 g/100 g), sulfate (2.4−11.5 g/100 g), small amounts of protein (<1−6.1 g/100 g), and nondialyzable polyphenols (0.1−2.7 g/100 g). The main neutral sugars were fucose, glucose, galactose, and xylose. Infrared (IR) spectra of the fractions showed absorption bands at 820−850 and 1225−1250 cm-1 for sulfate. F1, F2, and F4 also exhibited an absorption band at 1425 cm-1, due to uronic acids, and their IR spectra resembled that of alginate. F3 had an IR spectrum similar to that of fucoidan with an average molecular weight of 1.6 × 106 Da, calculated by molecular exclusion high-performance liquid chromatography. The presence of fucose in this polysaccharide was confirmed by 1H NMR spectroscopy. This fraction showed the highest potential to be antioxidant by the ...
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potential antioxidant capacity of sulfated polysaccharides from the edible marine brown seaweed Fucus vesiculosus
Journal of Agricultural and Food Chemistry, 2002Co-Authors: Pila Ruperez, Oussama Ahrazem, Antonio J LealAbstract:Fucus vesiculosus was sequentially extracted with water at 22 degrees C (fraction 1 (F1)) and 60 degrees C (F2), and with 0.1 M HCl (F3) and 2 M KOH (F4) at 37 degrees C. Soluble fractions (42.3% yield) were composed of neutral sugars (18.9-48 g/100 g), uronic acids (8.8-52.8 g/100 g), sulfate (2.4-11.5 g/100 g), small amounts of protein (< 1-6.1 g/100 g), and nondialyzable polyphenols (0.1-2.7 g/100 g). The main neutral sugars were fucose, glucose, galactose, and xylose. Infrared (IR) spectra of the fractions showed absorption bands at 820-850 and 1225-1250 cm(-1) for sulfate. F1, F2, and F4 also exhibited an absorption band at 1425 cm(-1), due to uronic acids, and their IR spectra resembled that of alginate. F3 had an IR spectrum similar to that of fucoidan with an average molecular weight of 1.6 x 10(6) Da, calculated by molecular exclusion high-performance liquid chromatography. The presence of fucose in this polysaccharide was confirmed by (1)H NMR spectroscopy. This fraction showed the highest potential to be antioxidant by the ferric reducing antioxidant power (FRAP) assay, followed by the alkali- and water-soluble fractions. Sulfated polysaccharides from edible seaweeds potentially could be used as natural antioxidants by the food industry.
Catarina Marçal - One of the best experts on this subject based on the ideXlab platform.
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Impact of Phlorotannin Extracts from Fucus vesiculosus on Human Gut Microbiota
MDPI AG, 2021Co-Authors: Marcelo D. Catarino, Catarina Marçal, Artur M. S. Silva, Teresa Bonifácio-lopes, Débora Campos, Nuno Mateus, Maria Manuela Pintado, Susana M. CardosoAbstract:Recent studies indicate that plant polyphenols could be pointed as potential prebiotic candidates since they may interact with the gut microbiota, stimulating its growth and the production of metabolites. However, little is known about the fate of brown seaweeds’ phlorotannins during their passage throughout the gastrointestinal tract. This work aimed to evaluate the stability and bioaccessibility of Fucus vesiculosus phlorotannins after being submitted to a simulated digestive process, as well as their possible modulatory effects on gut microbiota and short-chain fatty acids production following a fermentation procedure using fecal inoculates to mimic the conditions of the large intestine. The stability of phlorotannins throughout the gastrointestinal tract was reduced, with a bioaccessibility index between 2 and 14%. Moreover, slight alterations in the growth of certain commensal bacteria were noticed, with Enterococcus spp. being the most enhanced group. Likewise, F. vesiculosus phlorotannins displayed striking capacity to enhance the levels of propionate and butyrate, which are two important short-chain fatty acids known for their role in intestinal homeostasis. In summary, this work provides valuable information regarding the behavior of F. vesiculosus phlorotannins along the gastrointestinal tract, presenting clear evidence that these compounds can positively contribute to the maintenance of a healthy gastrointestinal condition.
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Microwave-Assisted Extraction of Phlorotannins from Fucus vesiculosus.
Marine drugs, 2020Co-Authors: Sónia J Amarante, Catarina Marçal, Artur M. S. Silva, Marcelo D. Catarino, Rita Ferreira, Susana M. CardosoAbstract:Microwave-assisted extraction (MAE) was carried out to maximize the extraction of phlorotannins from Fucus vesiculosus using a hydroethanolic mixture as a solvent, as an alternative to the conventional method with a hydroacetonic mixture. Optimal MAE conditions were set as ethanol concentration of 57% (v/v), temperature of 75 °C, and time of 5 min, which allowed a similar recovery of phlorotannins from the macroalgae compared to the conventional extraction. While the phlorotannins richness of the conventional extract was slightly superior to that of MAE (11.1 ± 1.3 vs. 9.8 ± 1.8 mg PGE/g DWextract), both extracts presented identical phlorotannins constituents, which included, among others, tetrafucol, pentafucol, hexafucol, and heptafucol structures. In addition, MAE showed a moderate capacity to scavenge ABTS•+ (IC50 of 96.0 ± 3.4 µg/mL) and to inhibit the activity of xanthine oxidase (IC50 of 23.1 ± 3.4 µg/mL) and a superior ability to control the activity of the key metabolic enzyme α-glucosidase compared to the pharmaceutical drug acarbose.
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Screening of Ulva rigida, Gracilaria sp., Fucus vesiculosus and Saccharina latissima as Functional Ingredients.
International journal of molecular sciences, 2018Co-Authors: Rodrigo T Neto, Catarina Marçal, Ana S Queirós, Helena Abreu, Artur M. S. Silva, Susana M. CardosoAbstract:The intent of the present work was to evaluate the potential of four macroalgae prevalent in Europe, namely Ulva rigida, Gracilaria sp., Fucus vesiculosus and Saccharina latissima, for application in functional foods, either in the direct form or as extracts. Accordingly, nutritional composition, the content of phytochemical antioxidants, and the inhibitory ability of key enzymes with impacts on obesity and diabetes (α-glucosidase and pancreatic lipase) or on arterial pressure (angiotensin-I converting enzyme), were evaluated. Overall, protein, lipid, ash and fiber contents of the macroalgae ranged from 9–24% dw, 0.5–3.0% dw, 20–32% dw, and 37–45% dw, respectively, making them good candidates for nutritional supplementation of several foods, particularly due to their mineral and fiber contents. In addition, brown macroalgae, in particular F. vesiculosus, stood out for its superior phenolic content, which was reflected by its high antioxidant ability and inhibition towards α-glucosidase activity (0.032 mg/mL of hydroacetonic extract inhibited 50% of the enzyme activity).
