The Experts below are selected from a list of 324 Experts worldwide ranked by ideXlab platform
Monika Bayrhuber - One of the best experts on this subject based on the ideXlab platform.
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nuclear magnetic resonance solution structure and Functional Behavior of the human proton channel
Biochemistry, 2019Co-Authors: Monika Bayrhuber, Innokentiy Maslennikov, Witek Kwiatkowski, A G Sobol, Christoph Wierschem, Cedric Eichmann, Lukas FreyAbstract:The human voltage-gated proton channel [Hv1(1) or VSDO(2)] plays an important role in the human innate immune system. Its structure differs considerably from those of other cation channels. It is built solely of a voltage-sensing domain and thus lacks the central pore domain, which is essential for other cation channels. Here, we determined the solution structure of an N- and C-terminally truncated human Hv1 (Δ-Hv1) in the resting state by nuclear magnetic resonance (NMR) spectroscopy. Δ-Hv1 comprises the typical voltage-sensing antiparallel four-helix bundle (S1–S4) preceded by an amphipathic helix (S0). The solution structure corresponds to an intermediate state between resting and activated forms of voltage-sensing domains. Furthermore, Zn2+-induced closing of proton channel Δ-Hv1 was studied with two-dimensional NMR spectroscopy, which showed that characteristic large scale dynamics of open Δ-Hv1 are absent in the closed state of the channel. Additionally, pH titration studies demonstrated that a high...
Lukas Frey - One of the best experts on this subject based on the ideXlab platform.
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nuclear magnetic resonance solution structure and Functional Behavior of the human proton channel
Biochemistry, 2019Co-Authors: Monika Bayrhuber, Innokentiy Maslennikov, Witek Kwiatkowski, A G Sobol, Christoph Wierschem, Cedric Eichmann, Lukas FreyAbstract:The human voltage-gated proton channel [Hv1(1) or VSDO(2)] plays an important role in the human innate immune system. Its structure differs considerably from those of other cation channels. It is built solely of a voltage-sensing domain and thus lacks the central pore domain, which is essential for other cation channels. Here, we determined the solution structure of an N- and C-terminally truncated human Hv1 (Δ-Hv1) in the resting state by nuclear magnetic resonance (NMR) spectroscopy. Δ-Hv1 comprises the typical voltage-sensing antiparallel four-helix bundle (S1–S4) preceded by an amphipathic helix (S0). The solution structure corresponds to an intermediate state between resting and activated forms of voltage-sensing domains. Furthermore, Zn2+-induced closing of proton channel Δ-Hv1 was studied with two-dimensional NMR spectroscopy, which showed that characteristic large scale dynamics of open Δ-Hv1 are absent in the closed state of the channel. Additionally, pH titration studies demonstrated that a high...
Innokentiy Maslennikov - One of the best experts on this subject based on the ideXlab platform.
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nuclear magnetic resonance solution structure and Functional Behavior of the human proton channel
Biochemistry, 2019Co-Authors: Monika Bayrhuber, Innokentiy Maslennikov, Witek Kwiatkowski, A G Sobol, Christoph Wierschem, Cedric Eichmann, Lukas FreyAbstract:The human voltage-gated proton channel [Hv1(1) or VSDO(2)] plays an important role in the human innate immune system. Its structure differs considerably from those of other cation channels. It is built solely of a voltage-sensing domain and thus lacks the central pore domain, which is essential for other cation channels. Here, we determined the solution structure of an N- and C-terminally truncated human Hv1 (Δ-Hv1) in the resting state by nuclear magnetic resonance (NMR) spectroscopy. Δ-Hv1 comprises the typical voltage-sensing antiparallel four-helix bundle (S1–S4) preceded by an amphipathic helix (S0). The solution structure corresponds to an intermediate state between resting and activated forms of voltage-sensing domains. Furthermore, Zn2+-induced closing of proton channel Δ-Hv1 was studied with two-dimensional NMR spectroscopy, which showed that characteristic large scale dynamics of open Δ-Hv1 are absent in the closed state of the channel. Additionally, pH titration studies demonstrated that a high...
Witek Kwiatkowski - One of the best experts on this subject based on the ideXlab platform.
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nuclear magnetic resonance solution structure and Functional Behavior of the human proton channel
Biochemistry, 2019Co-Authors: Monika Bayrhuber, Innokentiy Maslennikov, Witek Kwiatkowski, A G Sobol, Christoph Wierschem, Cedric Eichmann, Lukas FreyAbstract:The human voltage-gated proton channel [Hv1(1) or VSDO(2)] plays an important role in the human innate immune system. Its structure differs considerably from those of other cation channels. It is built solely of a voltage-sensing domain and thus lacks the central pore domain, which is essential for other cation channels. Here, we determined the solution structure of an N- and C-terminally truncated human Hv1 (Δ-Hv1) in the resting state by nuclear magnetic resonance (NMR) spectroscopy. Δ-Hv1 comprises the typical voltage-sensing antiparallel four-helix bundle (S1–S4) preceded by an amphipathic helix (S0). The solution structure corresponds to an intermediate state between resting and activated forms of voltage-sensing domains. Furthermore, Zn2+-induced closing of proton channel Δ-Hv1 was studied with two-dimensional NMR spectroscopy, which showed that characteristic large scale dynamics of open Δ-Hv1 are absent in the closed state of the channel. Additionally, pH titration studies demonstrated that a high...
Briley Proctor - One of the best experts on this subject based on the ideXlab platform.
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Evaluating the validity of Functional Behavior assessment
School Psychology Review, 2001Co-Authors: Mark D. Shriver, Cynthia M. Anderson, Briley ProctorAbstract:Abstract. This article provides a brief overview of Functional Behavior assessment and contrasts and compares Functional Behavior assessment and traditional psychological assessment. Messick's (1995) theory of construct validity is presented and then applied to the evaluation of the validity of Functional Behavior assessment measures. Finally, implications and guidelines of current construct validity theory are presented for school psychologists developing and/or using FBA measures and procedures. Following the most recent passage of the Individuals with Disabilities Education Act (IDEA, 1997), Functional Behavior assessment has received increasing attention in the educational and school psychology literature (Donovan, 1999; Dwyer, 1998; Nelson, Roberts, Bullis, Albers, & Ohland, 1999; Steege, 1999; Steege & Northup, 1998; Stewart, 1999). Although relatively new in education, Functional Behavior assessment has always been an integral component of applied Behavior analysis (Baer, Wolf, & Risley, 1968; Neef, 1994; Skinner, 1953). As educators and school psychologists continue to define and delineate of what Functional Behavior assessment will consist for their school districts, it will be necessary for them to look to the applied Behavior analysis literature for empirically supported methods and technology in Functional Behavior assessment. Functional Behavior assessment (FBA) incorporates many of the same assessment methods as more traditional psychological assessment. Interviews, Behavior rating forms, and observations are often key components of an FBA (e.g., Hintze & Eckert, 2001; O'Neill et al., 1997). However, the conceptual underpinnings of FBA differ substantially from those of more traditional psychological assessment (Hayes, Nelson, & Jarrett, 1986), and for this reason, applied Behavior analysts have not typically attended to many of the psychometric issues common to traditional psychological measurement (e.g., internal reliability, content validity, construct validity). These psychometric principles of measurement are well known to many school psychologists and are commonly used to evaluate traditional methods of assessment. School psychologists typically receive minimal training in applied Behavior analysis (Shriver & Watson, in press). Therefore, it may be difficult for school psychologists to evaluate the different FBA procedures and measures that are currently available given that these procedures were not developed within the same conceptual framework to which many psychometric principles of measurement are applicable. To provide guidance on evaluating FBA, this article provides a brief overview of FBA, contrasts and compares FBA and traditional psychological assessment, presents a model of validity developed for the evaluation of traditional psychological assessment data, and applies the validity model to the evaluation of FBA data. Finally, the implications for school psychologists developing and evaluating FBA measures and methods for their school district are presented. Functional Behavior Assessment FBA refers to the process of gathering information to determine relations between variables, particularly Functional relations. The term Functional refers to a demonstration of the effect of one variable on another variable, in other words cause-effect relations (Skinner, 1938, 1953). A description of a Functional relation may be correlational, although the term Functional in the Behavior analytic literature is usually reserved for cause-effect relations that have been experimentally demonstrated (Carr, 1994; Cone, 1997). Methods used to develop hypotheses regarding Functional relations include interviews with significant others (e.g., parents, teachers), interviews with the client (i.e., student), Behavior rating forms completed by others or the client, direct observation, and systematic manipulation of environmental events or contingencies (Iwata, Dorsey, Slifer, Bauman, & Richman, 1982/1994; O'Neill et al. …
