The Experts below are selected from a list of 135012 Experts worldwide ranked by ideXlab platform
Joel A Hirsch - One of the best experts on this subject based on the ideXlab platform.
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Expression, purification and crystallization of a Functional Core of the voltage-dependent calcium channel beta subunit.
Acta crystallographica. Section D Biological crystallography, 2004Co-Authors: Yarden Opatowsky, Orna Chomsky-hecht, Joel A HirschAbstract:Two versions of the Functional Core of the rabbit voltage-dependent calcium channel beta2a subunit were expressed in Escherichia coli. These proteins were purified to homogeneity and screened for crystallization. Crystallization conditions were refined using the hanging-drop vapour-diffusion method and two crystal forms were pursued. Crystal form I is represented by thick rods with tetragonal symmetry, unit-cell parameters a = b = 75, c = 165 A and a diffraction limit of 3.4 A which were obtained using ammonium sulfate as a precipitant. Crystal form II gives rise to plates with orthorhombic symmetry, unit-cell parameters a = 35, b = 75, c = 165 A and a diffraction limit of 2.3 A which were grown using polyethylene glycol 20K as a precipitant.
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Expression, purification and crystallization of a Functional Core of the voltage-dependent calcium channel β subunit
Acta Crystallographica Section D Biological Crystallography, 2004Co-Authors: Yarden Opatowsky, Orna Chomsky-hecht, Joel A HirschAbstract:Two versions of the Functional Core of the rabbit voltage-dependent calcium channel β2a subunit were expressed in Escherichia coli. These proteins were purified to homogeneity and screened for crystallization. Crystallization conditions were refined using the hanging-drop vapour-diffusion method and two crystal forms were pursued. Crystal form I is represented by thick rods with tetragonal symmetry, unit-cell parameters a = b = 75, c = 165 A and a diffraction limit of 3.4 A which were obtained using ammonium sulfate as a precipitant. Crystal form II gives rise to plates with orthorhombic symmetry, unit-cell parameters a = 35, b = 75, c = 165 A and a diffraction limit of 2.3 A which were grown using polyethylene glycol 20K as a precipitant.
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structural analysis of the voltage dependent calcium channel β subunit Functional Core and its complex with the α1 interaction domain
Neuron, 2004Co-Authors: Yarden Opatowsky, Chienchang Chen, Kevin P Campbell, Joel A HirschAbstract:Abstract Voltage-dependent calcium channels (VDCC) are multiprotein assemblies that regulate the entry of extracellular calcium into electrically excitable cells and serve as signal transduction centers. The α1 subunit forms the membrane pore while the intracellular β subunit is responsible for trafficking of the channel to the plasma membrane and modulation of its electrophysiological properties. Crystallographic analyses of a β subunit Functional Core alone and in complex with a α1 interaction domain (AID) peptide, the primary binding site of β to the α1 subunit, reveal that β represents a novel member of the MAGUK protein family. The findings illustrate how the guanylate kinase fold has been fashioned into a protein-protein interaction module by alteration of one of its substrate sites. Combined results indicate that the AID peptide undergoes a helical transition in binding to β. We outline the mechanistic implications for understanding the β subunit's broad regulatory role of the VDCC, particularly via the AID.
Yarden Opatowsky - One of the best experts on this subject based on the ideXlab platform.
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Expression, purification and crystallization of a Functional Core of the voltage-dependent calcium channel beta subunit.
Acta crystallographica. Section D Biological crystallography, 2004Co-Authors: Yarden Opatowsky, Orna Chomsky-hecht, Joel A HirschAbstract:Two versions of the Functional Core of the rabbit voltage-dependent calcium channel beta2a subunit were expressed in Escherichia coli. These proteins were purified to homogeneity and screened for crystallization. Crystallization conditions were refined using the hanging-drop vapour-diffusion method and two crystal forms were pursued. Crystal form I is represented by thick rods with tetragonal symmetry, unit-cell parameters a = b = 75, c = 165 A and a diffraction limit of 3.4 A which were obtained using ammonium sulfate as a precipitant. Crystal form II gives rise to plates with orthorhombic symmetry, unit-cell parameters a = 35, b = 75, c = 165 A and a diffraction limit of 2.3 A which were grown using polyethylene glycol 20K as a precipitant.
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Expression, purification and crystallization of a Functional Core of the voltage-dependent calcium channel β subunit
Acta Crystallographica Section D Biological Crystallography, 2004Co-Authors: Yarden Opatowsky, Orna Chomsky-hecht, Joel A HirschAbstract:Two versions of the Functional Core of the rabbit voltage-dependent calcium channel β2a subunit were expressed in Escherichia coli. These proteins were purified to homogeneity and screened for crystallization. Crystallization conditions were refined using the hanging-drop vapour-diffusion method and two crystal forms were pursued. Crystal form I is represented by thick rods with tetragonal symmetry, unit-cell parameters a = b = 75, c = 165 A and a diffraction limit of 3.4 A which were obtained using ammonium sulfate as a precipitant. Crystal form II gives rise to plates with orthorhombic symmetry, unit-cell parameters a = 35, b = 75, c = 165 A and a diffraction limit of 2.3 A which were grown using polyethylene glycol 20K as a precipitant.
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structural analysis of the voltage dependent calcium channel β subunit Functional Core and its complex with the α1 interaction domain
Neuron, 2004Co-Authors: Yarden Opatowsky, Chienchang Chen, Kevin P Campbell, Joel A HirschAbstract:Abstract Voltage-dependent calcium channels (VDCC) are multiprotein assemblies that regulate the entry of extracellular calcium into electrically excitable cells and serve as signal transduction centers. The α1 subunit forms the membrane pore while the intracellular β subunit is responsible for trafficking of the channel to the plasma membrane and modulation of its electrophysiological properties. Crystallographic analyses of a β subunit Functional Core alone and in complex with a α1 interaction domain (AID) peptide, the primary binding site of β to the α1 subunit, reveal that β represents a novel member of the MAGUK protein family. The findings illustrate how the guanylate kinase fold has been fashioned into a protein-protein interaction module by alteration of one of its substrate sites. Combined results indicate that the AID peptide undergoes a helical transition in binding to β. We outline the mechanistic implications for understanding the β subunit's broad regulatory role of the VDCC, particularly via the AID.
Denis L.j. Lafontaine - One of the best experts on this subject based on the ideXlab platform.
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Noncoding RNAs in eukaryotic ribosome biogenesis and function
Nature structural & molecular biology, 2015Co-Authors: Denis L.j. LafontaineAbstract:The ribosome, central to protein synthesis in all cells, is a complex multicomponent assembly with rRNA at its Functional Core. During the process of ribosome biogenesis, diverse noncoding RNAs participate in controlling the quantity and quality of this rRNA. In this Review, I discuss the multiple roles assumed by noncoding RNAs during the different steps of ribosome biogenesis and how they contribute to the generation of ribosome heterogeneity, which affects normal and pathophysiological processes.
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Noncoding RNAs in eukaryotic ribosome biogenesis and function
Nature Structural & Molecular Biology, 2015Co-Authors: Denis L.j. LafontaineAbstract:The ribosome is a complex molecular machine that is central to protein synthesis. This Review highlights the various roles of noncoding RNAs during the different steps of ribosome biogenesis and discusses the consequences for ribosome function. The ribosome, central to protein synthesis in all cells, is a complex multicomponent assembly with rRNA at its Functional Core. During the process of ribosome biogenesis, diverse noncoding RNAs participate in controlling the quantity and quality of this rRNA. In this Review, I discuss the multiple roles assumed by noncoding RNAs during the different steps of ribosome biogenesis and how they contribute to the generation of ribosome heterogeneity, which affects normal and pathophysiological processes.
Leonie Barner - One of the best experts on this subject based on the ideXlab platform.
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Facile Access to Hydroxy‐Functional Core–Shell Microspheres via Grafting of Ethylene Oxide by Anionic Ring‐Opening Polymerization
Macromolecular rapid communications, 2009Co-Authors: Raymond Joso, Stefan Reinicke, Andreas Walther, Holger Schmalz, Axel H. E. Müller, Leonie BarnerAbstract:We present a facile access route to hydroxy-Functional narrow disperse microspheres of well-defined grafting density (GD). Ethylene oxide has been grafted from highly crosslinked poly(divinyl benzene) microspheres by anionic ring-opening polymerization using sec-butyllithium as activator together with the phosphazene base t-BuP(4) . Initially, Core microspheres have been prepared by precipitation polymerization utilizing divinyl benzene (DVB, 80 wt.-%). The grafting of poly(ethylene oxide) (PEO) from the surface resulted in the formation of Functional Core-shell microspheres with hydroxy-terminal end groups. The number average particle diameter of the grafted microspheres was 3.6 µm and the particle weight increased by 5.7%. The microspheres were characterized by SEM, FT-IR spectroscopy, elemental analysis, and fluorescence microscopy. The surface GD (determined via two methods) was 1.65 ± 0.06 and 2.09 ± 0.08 chains · nm(-2) , respectively.
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Facile access to hydroxy-Functional Core-shell microspheres via grafting of ethylene oxide by anionic ring-opening polymerization
2009Co-Authors: Raymond Joso, Stefan Reinicke, Andreas Walther, Holger Schmalz, Axel H.e. Mueller, Leonie BarnerAbstract:We present a facile access route to hydroxy-Functional narrow disperse microspheres of well-defined grafting density (GD). Ethylene oxide has been grafted from highly crosslinked poly(divinyl benzene) microspheres by anionic ring-opening polymerization using sec-butyllithium as activator together with the phosphazene base t -BuP 4 . Initially, Core microspheres have been prepared by precipitation polymerization utilizing divinyl benzene (DVB, 80 wt.-%). The grafting of poly(ethylene oxide) (PEO) from the surface resulted in the formation of Functional Core-shell microspheres with hydroxy-terminal end groups. The number average particle diameter of the grafted microspheres was 3.6 mu m and the particle weight increased by 5.7%. The microspheres were characterized by SEM, FT-IR spectroscopy, elemental analysis, and fluorescence microscopy. The surface GD (determined via two methods) was 1.65 +/- 0.06 and 2.09 +/- 0.08 chains.nm -2 , respectively.
Albert Gollhofer - One of the best experts on this subject based on the ideXlab platform.
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Influence of a Functional Core Stability Program on Trunk and Knee Joint Biomechanics in Female Athletes During Lateral Movements.
Journal of strength and conditioning research, 2019Co-Authors: Guillaume Mornieux, Elmar Weltin, Craig Friedman, Monika Pauls, Shad Forsythe, Albert GollhoferAbstract:Mornieux, G, Weltin, E, Friedman, C, Pauls, M, Forsythe, S, and Gollhofer, A. Influence of a Functional Core stability program on trunk and knee joint biomechanics in female athletes during lateral movements. J Strength Cond Res XX(X): 000-000, 2019-Trunk positioning has been shown to be associated with knee joint loading during athletic tasks, especially changes of direction. Quasistatic and conventional Core stability (CS) training did not improve the biomechanics of cutting maneuvers according to reports in the literature. The purpose of this study was to test whether a 5-week dynamic and Functional CS training program would improve trunk positioning and knee joint control during lateral movements. Nineteen female athletes participated in a longitudinal study, where lateral reactive jumps (LRJs) and unanticipated cuttings were performed before and after CS training (15 training sessions within 5 weeks) while 3D kinematics and kinetics were measured. In addition, Core endurance and power were evaluated. Core stability training increased trunk rotation in the new movement direction (p = 0.02) during LRJ. However, lateral trunk lean was significantly increased during LRJ (from 5.6 to 7°) and cuttings (from 3.8 to 5.8°) after CS training. Knee joint abduction moment was not influenced. Finally, Core endurance (p < 0.01) and Core power (p < 0.001) were significantly improved after CS training. A 5-week dynamic and Functional CS training program improved Core muscle endurance and power. This might explain the better trunk rotation toward the new movement direction during LRJ; however, it was not effective during unanticipated cuttings. The practical application of this research will allow strength and conditioning professionals to setup training programs with simple and relevant exercises to increase the Core endurance and power of trained individuals.
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Influence of a Functional Core Stability Program on Trunk and Knee Joint Biomechanics in Female Athletes During Lateral Movements
Journal of Strength and Conditioning Research, 2019Co-Authors: Guillaume Mornieux, Elmar Weltin, Craig Friedman, Monika Pauls, Shad Forsythe, Albert GollhoferAbstract:Influence of a Functional Core stability program on trunk and knee joint biomechanics in female athletes during lateral movements. J Strength Cond Res XX(X): 000-000, 2019-Trunk positioning has been shown to be associated with knee joint loading during athletic tasks, especially changes of direction. Quasistatic and conventional Core stability (CS) training did not improve the biomechanics of cutting maneuvers according to reports in the literature. The purpose of this study was to test whether a 5-week dynamic and Functional CS training program would improve trunk positioning and knee joint control during lateral movements. Nineteen female athletes participated in a longitudinal study, where lateral reactive jumps (LRJs) and unanticipated cuttings were performed before and after CS training (15 training sessions within 5 weeks) while 3D kinematics and kinetics were measured. In addition, Core endurance and power were evaluated. Core stability training increased trunk rotation in the new movement direction (p = 0.02) during LRJ. However, lateral trunk lean was significantly increased during LRJ (from 5.6 to 7°) and cuttings (from 3.8 to 5.8°) after CS training. Knee joint abduction moment was not influenced. Finally, Core endurance (p < 0.01) and Core power (p < 0.001) were significantly improved after CS training. A 5-week dynamic and Functional CS training program improved Core muscle endurance and power. This might explain the better trunk rotation toward the new movement direction during LRJ; however, it was not effective during unanticipated cuttings. The practical application of this research will allow strength and conditioning professionals to setup training programs with simple and relevant exercises to increase the Core endurance and power of trained individuals.
