The Experts below are selected from a list of 58890 Experts worldwide ranked by ideXlab platform
Xiaoxin Chen - One of the best experts on this subject based on the ideXlab platform.
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Transgenic overexpression of cdx1b induces metaplastic changes of gene expression in zebrafish esophageal squamous epithelium.
Zebrafish, 2013Co-Authors: Bo Hu, Gregory J. Cole, Hao Chen, Chengjin Zhang, Xiaoxin ChenAbstract:Abstract Cdx2 has been suggested to play an important role in Barrett's esophagus or intestinal metaplasia (IM) in the esophagus. To investigate whether transgenic overexpression of cdx1b, the Functional Equivalent of mammalian Cdx2 in zebrafish, may lead to IM of zebrafish esophageal squamous epithelium, a transgenic zebrafish system was developed by expressing cdx1b gene under the control of zebrafish keratin 5 promoter (krt5p). Gene expression in the esophageal squamous epithelium of wild-type and transgenic zebrafish was analyzed by Affymetrix microarray and confirmed by in situ hybridization. Morphology, mucin expression, cell proliferation, and apoptosis were analyzed by hematoxylin & eosin (HE) staining, Periodic acid Schiff (PAS) Alcian blue staining, proliferating cell nuclear antigen (PCNA) immunohistochemical staining, and TUNEL assay as well. cdx1b was found to be overexpressed in the nuclei of esophageal squamous epithelial cells of the transgenic zebrafish. Ectopic expression of cdx1b distur...
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Abstract 1960: Transgenic overexpression of Cdx1b in zebrafish induces metaplastic changes of gene expression in the squamous epithelium
Cancer Research, 2010Co-Authors: Bo Hu, Gregory J. Cole, Chengjin Zhang, Xiaoxin ChenAbstract:Background and aims: Cdx2 has been suggested to play an important role in Barrett9s esophagus (BE), or intestinal metaplasia (IM) in the esophagus. However, it is still unclear whether ectopic overexpression of Cdx2 may lead to IM in the esophagus in vivo. The aim of the present study was to investigate whether transgenic overexpression of Cdx1b, the Functional Equivalent of mammalian Cdx2 in zebrafish, may lead to IM of squamous epithelium in zebrafish. Methods: We first characterized the histology of the upper gastrointestinal tract of zebrafish. A highly conserved promoter of zebrafish keratin 5 was cloned to drive transgenic expression of GFP. A transgenic zebrafish system was then developed by expressing Cdx1b gene under the control of zebrafish keratin 5 promoter (zK5p). Cdx1b expression in the squamous epithelium of transgenic zebrafish was analyzed by in situ hybridization, immunohistochemical staining and RT-PCR. Gene expression in the squamous epithelium of wild-type and transgenic zebrafish was analyzed by Affymetrix microarray and confirmed by in situ hybridization. Morphology, mucin expression, cell proliferation and apoptosis were analyzed by HE 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 1960.
John V Kilmartin - One of the best experts on this subject based on the ideXlab platform.
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lessons from yeast the spindle pole body and the centrosome
Philosophical Transactions of the Royal Society B, 2014Co-Authors: John V KilmartinAbstract:The yeast spindle pole body (SPB) is the Functional Equivalent of the centrosome. Most SPB components have been identified and their functions partly established. This involved a large variety of techniques which are described here, and the potential use of some of these in the centrosome field is highlighted. In particular, very useful structural information on the SPB was obtained from a reconstituted complex, the γ-tubulin complex, and also from a sub-particle, SPB cores, prepared by extraction of an enriched SPB preparation. The labelling of SPB proteins with GFP at the N or C termini, using GFP tags inserted into the genome, gave informative electron microscopy localization and fluorescence resonance energy transfer data. Examples are given of more precise Functional data obtained by removing domains from one SPB protein, Spc110p, without affecting its essential function. Finally, a structural model for SPB duplication is described and the differences between SPB and centrosome duplication discussed.
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spindle pole body duplication a model for centrosome duplication
Trends in Cell Biology, 2000Co-Authors: Ian R. Adams, John V KilmartinAbstract:The yeast spindle pole body (SPB) is the Functional Equivalent of the centrosome and forms the two poles of the mitotic spindle. Before mitosis, both SPBs and centrosomes are present as single copies and must be duplicated to form the bipolar spindle. SPB components have been identified using a combination of biochemistry and genetics, and their role during SPB duplication has been analysed using temperature-sensitive mutants. In this article, we describe structural aspects of SPB duplication and their possible relationship to centrosome duplication.
Bo Hu - One of the best experts on this subject based on the ideXlab platform.
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Transgenic overexpression of cdx1b induces metaplastic changes of gene expression in zebrafish esophageal squamous epithelium.
Zebrafish, 2013Co-Authors: Bo Hu, Gregory J. Cole, Hao Chen, Chengjin Zhang, Xiaoxin ChenAbstract:Abstract Cdx2 has been suggested to play an important role in Barrett's esophagus or intestinal metaplasia (IM) in the esophagus. To investigate whether transgenic overexpression of cdx1b, the Functional Equivalent of mammalian Cdx2 in zebrafish, may lead to IM of zebrafish esophageal squamous epithelium, a transgenic zebrafish system was developed by expressing cdx1b gene under the control of zebrafish keratin 5 promoter (krt5p). Gene expression in the esophageal squamous epithelium of wild-type and transgenic zebrafish was analyzed by Affymetrix microarray and confirmed by in situ hybridization. Morphology, mucin expression, cell proliferation, and apoptosis were analyzed by hematoxylin & eosin (HE) staining, Periodic acid Schiff (PAS) Alcian blue staining, proliferating cell nuclear antigen (PCNA) immunohistochemical staining, and TUNEL assay as well. cdx1b was found to be overexpressed in the nuclei of esophageal squamous epithelial cells of the transgenic zebrafish. Ectopic expression of cdx1b distur...
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Abstract 1960: Transgenic overexpression of Cdx1b in zebrafish induces metaplastic changes of gene expression in the squamous epithelium
Cancer Research, 2010Co-Authors: Bo Hu, Gregory J. Cole, Chengjin Zhang, Xiaoxin ChenAbstract:Background and aims: Cdx2 has been suggested to play an important role in Barrett9s esophagus (BE), or intestinal metaplasia (IM) in the esophagus. However, it is still unclear whether ectopic overexpression of Cdx2 may lead to IM in the esophagus in vivo. The aim of the present study was to investigate whether transgenic overexpression of Cdx1b, the Functional Equivalent of mammalian Cdx2 in zebrafish, may lead to IM of squamous epithelium in zebrafish. Methods: We first characterized the histology of the upper gastrointestinal tract of zebrafish. A highly conserved promoter of zebrafish keratin 5 was cloned to drive transgenic expression of GFP. A transgenic zebrafish system was then developed by expressing Cdx1b gene under the control of zebrafish keratin 5 promoter (zK5p). Cdx1b expression in the squamous epithelium of transgenic zebrafish was analyzed by in situ hybridization, immunohistochemical staining and RT-PCR. Gene expression in the squamous epithelium of wild-type and transgenic zebrafish was analyzed by Affymetrix microarray and confirmed by in situ hybridization. Morphology, mucin expression, cell proliferation and apoptosis were analyzed by HE 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 1960.
Chengjin Zhang - One of the best experts on this subject based on the ideXlab platform.
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Transgenic overexpression of cdx1b induces metaplastic changes of gene expression in zebrafish esophageal squamous epithelium.
Zebrafish, 2013Co-Authors: Bo Hu, Gregory J. Cole, Hao Chen, Chengjin Zhang, Xiaoxin ChenAbstract:Abstract Cdx2 has been suggested to play an important role in Barrett's esophagus or intestinal metaplasia (IM) in the esophagus. To investigate whether transgenic overexpression of cdx1b, the Functional Equivalent of mammalian Cdx2 in zebrafish, may lead to IM of zebrafish esophageal squamous epithelium, a transgenic zebrafish system was developed by expressing cdx1b gene under the control of zebrafish keratin 5 promoter (krt5p). Gene expression in the esophageal squamous epithelium of wild-type and transgenic zebrafish was analyzed by Affymetrix microarray and confirmed by in situ hybridization. Morphology, mucin expression, cell proliferation, and apoptosis were analyzed by hematoxylin & eosin (HE) staining, Periodic acid Schiff (PAS) Alcian blue staining, proliferating cell nuclear antigen (PCNA) immunohistochemical staining, and TUNEL assay as well. cdx1b was found to be overexpressed in the nuclei of esophageal squamous epithelial cells of the transgenic zebrafish. Ectopic expression of cdx1b distur...
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Abstract 1960: Transgenic overexpression of Cdx1b in zebrafish induces metaplastic changes of gene expression in the squamous epithelium
Cancer Research, 2010Co-Authors: Bo Hu, Gregory J. Cole, Chengjin Zhang, Xiaoxin ChenAbstract:Background and aims: Cdx2 has been suggested to play an important role in Barrett9s esophagus (BE), or intestinal metaplasia (IM) in the esophagus. However, it is still unclear whether ectopic overexpression of Cdx2 may lead to IM in the esophagus in vivo. The aim of the present study was to investigate whether transgenic overexpression of Cdx1b, the Functional Equivalent of mammalian Cdx2 in zebrafish, may lead to IM of squamous epithelium in zebrafish. Methods: We first characterized the histology of the upper gastrointestinal tract of zebrafish. A highly conserved promoter of zebrafish keratin 5 was cloned to drive transgenic expression of GFP. A transgenic zebrafish system was then developed by expressing Cdx1b gene under the control of zebrafish keratin 5 promoter (zK5p). Cdx1b expression in the squamous epithelium of transgenic zebrafish was analyzed by in situ hybridization, immunohistochemical staining and RT-PCR. Gene expression in the squamous epithelium of wild-type and transgenic zebrafish was analyzed by Affymetrix microarray and confirmed by in situ hybridization. Morphology, mucin expression, cell proliferation and apoptosis were analyzed by HE 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 1960.
Gregory J. Cole - One of the best experts on this subject based on the ideXlab platform.
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Transgenic overexpression of cdx1b induces metaplastic changes of gene expression in zebrafish esophageal squamous epithelium.
Zebrafish, 2013Co-Authors: Bo Hu, Gregory J. Cole, Hao Chen, Chengjin Zhang, Xiaoxin ChenAbstract:Abstract Cdx2 has been suggested to play an important role in Barrett's esophagus or intestinal metaplasia (IM) in the esophagus. To investigate whether transgenic overexpression of cdx1b, the Functional Equivalent of mammalian Cdx2 in zebrafish, may lead to IM of zebrafish esophageal squamous epithelium, a transgenic zebrafish system was developed by expressing cdx1b gene under the control of zebrafish keratin 5 promoter (krt5p). Gene expression in the esophageal squamous epithelium of wild-type and transgenic zebrafish was analyzed by Affymetrix microarray and confirmed by in situ hybridization. Morphology, mucin expression, cell proliferation, and apoptosis were analyzed by hematoxylin & eosin (HE) staining, Periodic acid Schiff (PAS) Alcian blue staining, proliferating cell nuclear antigen (PCNA) immunohistochemical staining, and TUNEL assay as well. cdx1b was found to be overexpressed in the nuclei of esophageal squamous epithelial cells of the transgenic zebrafish. Ectopic expression of cdx1b distur...
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Abstract 1960: Transgenic overexpression of Cdx1b in zebrafish induces metaplastic changes of gene expression in the squamous epithelium
Cancer Research, 2010Co-Authors: Bo Hu, Gregory J. Cole, Chengjin Zhang, Xiaoxin ChenAbstract:Background and aims: Cdx2 has been suggested to play an important role in Barrett9s esophagus (BE), or intestinal metaplasia (IM) in the esophagus. However, it is still unclear whether ectopic overexpression of Cdx2 may lead to IM in the esophagus in vivo. The aim of the present study was to investigate whether transgenic overexpression of Cdx1b, the Functional Equivalent of mammalian Cdx2 in zebrafish, may lead to IM of squamous epithelium in zebrafish. Methods: We first characterized the histology of the upper gastrointestinal tract of zebrafish. A highly conserved promoter of zebrafish keratin 5 was cloned to drive transgenic expression of GFP. A transgenic zebrafish system was then developed by expressing Cdx1b gene under the control of zebrafish keratin 5 promoter (zK5p). Cdx1b expression in the squamous epithelium of transgenic zebrafish was analyzed by in situ hybridization, immunohistochemical staining and RT-PCR. Gene expression in the squamous epithelium of wild-type and transgenic zebrafish was analyzed by Affymetrix microarray and confirmed by in situ hybridization. Morphology, mucin expression, cell proliferation and apoptosis were analyzed by HE 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 1960.
