The Experts below are selected from a list of 17103 Experts worldwide ranked by ideXlab platform
J A Ordonez - One of the best experts on this subject based on the ideXlab platform.
-
combined use of pronase e and a Fungal Extract penicillium aurantiogriseum to potentiate the sensory characteristics of dry fermented sausages
Meat Science, 2000Co-Authors: Jose M Bruna, Eva Hierro, Manuela Fernandez, J A OrdonezAbstract:Abstract A combination of an Extract of Penicillium aurantiogriseum and Pronase E was used in an attempt to enhance the sensory characteristics of dry fermented sausages, specifically, a salami-like one named “salchichon”. The addition of Pronase E alone (600 units/kg) produced a rise in free amino acids and biogenic amines and also an increase in the ammonia content. Addition of the protease and Fungal Extract (100.87 mg protein/kg of mixture) brought about a decrease in the level of free amino acids and a larger increase in the ammonia content than the batch added with only Pronase E. There was also an increase in the amount of specific volatile compounds such as 2- and 3-methylbutanal, 2- and 3-methylpropanal and 2- and 3-methyl-1-butanol produced by the breakdown of amino acids in these fermented sausages. Values of pH, a w and dry matter were not affected by addition of the protease or Fungal Extract. In contrast, addition of Pronase E produced a notable change in the textural characteristics, reducing the hardness, cohesiveness, gumminess, chewiness and cutting force. Sensory analysis showed that fermented sausages manufactured with Pronase E and Penicillium aurantiogriseum Extract had better odour, flavour, texture and, as a consequence, better general acceptability.
-
efecto del uso combinado de la pronasa e y de un Extracto fungico mucor racemosus forma sphaerosporus en la maduracion de embutidos crudos curados effect of the combined use of pronase e and a Fungal Extract mucor racemosus forma sphaerosporus on the
Food Science and Technology International, 1999Co-Authors: Jose M Bruna, Eva Hierro, Manuela Fernandez, J A OrdonezAbstract:The effect of the addition of 600 units of Pronase E/kg of sausage mixture and/or a Fungal Extract ( Mucor racemosus forma sphaerosporus) at two different concentrations (1.75 and 17.5 mg protein/kg sausage mixture) on dry fermented sausages was evaluated. The only addition of protease to sau sages led to an increase in the content of free amino acids and biogenic amines. When the protease and the Fungal Extract were added together at high concentration (17.5 mg protein/kg sausage mix ture), a decrease in the free amino acid content and a slight increase in the ammonia content were observed, together with a clear increase in the concentration of several volatile compounds, such as 2- and 3-methylbutanal, 2-methylpropanal and 2- and 3-methyl-1-butanol. The addition of the Fungal Extract only at high concentration (17.5 mg protein/kg sausage mixture) or the combined protease and Fungal Extract at low concentration (1.75 mg protein/kg sausage mixture), produced slight physi cochemical modifications compared ...
Tasdemir Deniz - One of the best experts on this subject based on the ideXlab platform.
-
Pyrenosetins A–C, new decalinoylspirotetramic acid derivatives isolated by bioactivity-based molecular networking from the seaweed-derived fungus Pyrenochaetopsis sp. FVE-001
'MDPI AG', 2020Co-Authors: Fan Bicheng, Dewapriya Pradeep, Li Fengjie, Blümel Martina, Tasdemir DenizAbstract:Marine algae represent a prolific source of filamentous fungi for bioprospecting. In continuation of our search for new anticancer leads from fungi derived from the brown alga , an endophytic sp. FVE-001 was selected for an in-depth chemical analysis. The crude Fungal Extract inhibited several cancer cell lines in vitro, and the highest anticancer activity was tracked to its CHCl-soluble portion. A bioactivity-based molecular networking approach was applied to C-SPE fractions of the CHCl subExtract to predict the bioactivity scores of metabolites in the fractions and to aid targeted purification of anticancer metabolites. This approach led to a rapid isolation of three new decalinoylspirotetramic acid derivatives, pyrenosetins A-C (-) and the known decalin tetramic acid phomasetin (). The structures of the compounds were elucidated by extensive NMR, HR-ESIMS, FT-IR spectroscopy, [α] and Mosher's ester method. Compounds and showed high anticancer activity against malignant melanoma cell line A-375 (IC values 2.8 and 6.3 μM, respectively), in line with the bioactivity predictions. This is the first study focusing on secondary metabolites of a marine-derived sp. and the second investigation performed on the member of the genus
-
Pyrenosetins A-C, New Decalinoylspirotetramic Acid Derivatives Isolated by Bioactivity-Based Molecular Networking from the Seaweed-Derived Fungus Pyrenochaetopsis sp. FVE-001
'MDPI AG', 2020Co-Authors: Fan Bicheng, Dewapriya Pradeep, Li Fengjie, Blümel Martina, Tasdemir DenizAbstract:Marine algae represent a prolific source of filamentous fungi for bioprospecting. In continuation of our search for new anticancer leads from fungi derived from the brown alga Fucus vesiculosus, an endophytic Pyrenochaetopsis sp. FVE-001 was selected for an in-depth chemical analysis. The crude Fungal Extract inhibited several cancer cell lines in vitro, and the highest anticancer activity was tracked to its CHCl3-soluble portion. A bioactivity-based molecular networking approach was applied to C18-SPE fractions of the CHCl3 subExtract to predict the bioactivity scores of metabolites in the fractions and to aid targeted purification of anticancer metabolites. This approach led to a rapid isolation of three new decalinoylspirotetramic acid derivatives, pyrenosetins A-C (1-3) and the known decalin tetramic acid phomasetin (4). The structures of the compounds were elucidated by extensive NMR, HR-ESIMS, FT-IR spectroscopy, [α]D and Mosher's ester method. Compounds 1 and 2 showed high anticancer activity against malignant melanoma cell line A-375 (IC50 values 2.8 and 6.3 μM, respectively), in line with the bioactivity predictions. This is the first study focusing on secondary metabolites of a marine-derived Pyrenochaetopsis sp. and the second investigation performed on the member of the genus Pyrenochaetopsis
-
Pyrenosetins A–C, New Decalinoylspirotetramic Acid Derivatives Isolated by Bioactivity-Based Molecular Networking from the Seaweed-Derived Fungus Pyrenochaetopsis sp. FVE-001
'MDPI AG', 2020Co-Authors: Fan Bicheng, Dewapriya Pradeep, Li Fengjie, Blümel Martina, Tasdemir DenizAbstract:Marine algae represent a prolific source of filamentous fungi for bioprospecting. In continuation of our search for new anticancer leads from fungi derived from the brown alga Fucus vesiculosus, an endophytic Pyrenochaetopsis sp. FVE-001 was selected for an in-depth chemical analysis. The crude Fungal Extract inhibited several cancer cell lines in vitro, and the highest anticancer activity was tracked to its CHCl3–soluble portion. A bioactivity-based molecular networking approach was applied to C18-SPE fractions of the CHCl3 subExtract to predict the bioactivity scores of metabolites in the fractions and to aid targeted purification of anticancer metabolites. This approach led to a rapid isolation of three new decalinoylspirotetramic acid derivatives, pyrenosetins A–C (1–3) and the known decalin tetramic acid phomasetin (4). The structures of the compounds were elucidated by extensive NMR, HR-ESIMS, FT-IR spectroscopy, [α]D and Mosher’s ester method. Compounds 1 and 2 showed high anticancer activity against malignant melanoma cell line A-375 (IC50 values 2.8 and 6.3 μM, respectively), in line with the bioactivity predictions. This is the first study focusing on secondary metabolites of a marine-derived Pyrenochaetopsis sp. and the second investigation performed on the member of the genus Pyrenochaetopsis
Eric A. Johnson - One of the best experts on this subject based on the ideXlab platform.
-
2004. Stimulation of astaxanthin formation in the yeast Xanthophyllomyces dendrorhous by the fungus Epicoccum nigrum. FEMS Yeast Res
2016Co-Authors: Carlos Echavarri-erasun, Eric A. JohnsonAbstract:A Fungal contaminant on an agar plate containing colonies of Xanthophyllomyces dendrorhous markedly increased carotenoid production by yeast colonies near to the Fungal growth. Spent-culture filtrate from growth of the fungus in yeast^malt medium also stimulated carotenoid production by X. dendrorhous. Four X. dendrorhous strains including the wild-type UCD 67-385 (ATCC 24230), AF-1 (albino mutant, ATCC 96816), Yan-1 (L-carotene mutant, ATCC 96815) and CAX (astaxanthin overproducer mutant) exposed to Fungal concentrate Extract enhanced astaxanthin up to approximately 40 % per unit dry cell weight in the wild-type strain and in CAX. Interestingly, the Fungal Extract restored astaxanthin biosynthesis in non-astaxanthin-producing mutants previously isolated in our laboratory, including the albino and the L-carotene mutant. The fungus was identified as Epicoccum nigrum by morphology of sporulating cultures, and the identity confirmed by genetic characterization including rDNA sequencing analysis of the large-subunit (LSU), the internal transcribed spacer, and the D1/D2 region of the LSU. These E. nigrum rDNA sequences were deposited in GenBank under accesssion numbers AF338443, AY093413 and AY093414. Systematic rDNA homology alignments were performed to identify fungi related to E. nigrum. Stimulation of carotenogenesis by E. nigrum and potentially other fungi could provide a novel method to enhance astaxanthin formation in industrial fermentations of X. dendrorhous and Phaffia rhodozyma.> 2003 Published by Elsevier B.V. on behalf of the Federation of European Microbiological Societies
-
stimulation of astaxanthin formation in the yeast xanthophyllomyces dendrorhous by the fungus epicoccum nigrum
Fems Yeast Research, 2004Co-Authors: Carlos Echavarrierasun, Eric A. JohnsonAbstract:A Fungal contaminant on an agar plate containing colonies of Xanthophyllomyces dendrorhous markedly increased carotenoid production by yeast colonies near to the Fungal growth. Spent-culture filtrate from growth of the fungus in yeast–malt medium also stimulated carotenoid production by X. dendrorhous . Four X. dendrorhous strains including the wild-type UCD 67-385 (ATCC 24230), AF-1 (albino mutant, ATCC 96816), Yan-1 (β-carotene mutant, ATCC 96815) and CAX (astaxanthin overproducer mutant) exposed to Fungal concentrate Extract enhanced astaxanthin up to approximately 40% per unit dry cell weight in the wild-type strain and in CAX. Interestingly, the Fungal Extract restored astaxanthin biosynthesis in non-astaxanthin-producing mutants previously isolated in our laboratory, including the albino and the β-carotene mutant. The fungus was identified as Epicoccum nigrum by morphology of sporulating cultures, and the identity confirmed by genetic characterization including rDNA sequencing analysis of the large-subunit (LSU), the internal transcribed spacer, and the D1/D2 region of the LSU. These E. nigrum rDNA sequences were deposited in GenBank under accesssion numbers [AF338443][1], [AY093413][2] and [AY093414][3]. Systematic rDNA homology alignments were performed to identify fungi related to E. nigrum . Stimulation of carotenogenesis by E. nigrum and potentially other fungi could provide a novel method to enhance astaxanthin formation in industrial fermentations of X. dendrorhous and Phaffia rhodozyma . * Abbreviations : rDNA : ribosomal DNA ITS : internal transcribed spacer LSU : large-subunit 1O2 : singlet oxygen YM : yeast–malt broth gCDW : gram cell dry weight [1]: /lookup/external-ref?link_type=GEN&access_num=AF338443&atom=%2Ffemsyr%2F4%2F4-5%2F511.atom [2]: /lookup/external-ref?link_type=GEN&access_num=AY093413&atom=%2Ffemsyr%2F4%2F4-5%2F511.atom [3]: /lookup/external-ref?link_type=GEN&access_num=AY093414&atom=%2Ffemsyr%2F4%2F4-5%2F511.atom
Jose M Bruna - One of the best experts on this subject based on the ideXlab platform.
-
combined use of pronase e and a Fungal Extract penicillium aurantiogriseum to potentiate the sensory characteristics of dry fermented sausages
Meat Science, 2000Co-Authors: Jose M Bruna, Eva Hierro, Manuela Fernandez, J A OrdonezAbstract:Abstract A combination of an Extract of Penicillium aurantiogriseum and Pronase E was used in an attempt to enhance the sensory characteristics of dry fermented sausages, specifically, a salami-like one named “salchichon”. The addition of Pronase E alone (600 units/kg) produced a rise in free amino acids and biogenic amines and also an increase in the ammonia content. Addition of the protease and Fungal Extract (100.87 mg protein/kg of mixture) brought about a decrease in the level of free amino acids and a larger increase in the ammonia content than the batch added with only Pronase E. There was also an increase in the amount of specific volatile compounds such as 2- and 3-methylbutanal, 2- and 3-methylpropanal and 2- and 3-methyl-1-butanol produced by the breakdown of amino acids in these fermented sausages. Values of pH, a w and dry matter were not affected by addition of the protease or Fungal Extract. In contrast, addition of Pronase E produced a notable change in the textural characteristics, reducing the hardness, cohesiveness, gumminess, chewiness and cutting force. Sensory analysis showed that fermented sausages manufactured with Pronase E and Penicillium aurantiogriseum Extract had better odour, flavour, texture and, as a consequence, better general acceptability.
-
efecto del uso combinado de la pronasa e y de un Extracto fungico mucor racemosus forma sphaerosporus en la maduracion de embutidos crudos curados effect of the combined use of pronase e and a Fungal Extract mucor racemosus forma sphaerosporus on the
Food Science and Technology International, 1999Co-Authors: Jose M Bruna, Eva Hierro, Manuela Fernandez, J A OrdonezAbstract:The effect of the addition of 600 units of Pronase E/kg of sausage mixture and/or a Fungal Extract ( Mucor racemosus forma sphaerosporus) at two different concentrations (1.75 and 17.5 mg protein/kg sausage mixture) on dry fermented sausages was evaluated. The only addition of protease to sau sages led to an increase in the content of free amino acids and biogenic amines. When the protease and the Fungal Extract were added together at high concentration (17.5 mg protein/kg sausage mix ture), a decrease in the free amino acid content and a slight increase in the ammonia content were observed, together with a clear increase in the concentration of several volatile compounds, such as 2- and 3-methylbutanal, 2-methylpropanal and 2- and 3-methyl-1-butanol. The addition of the Fungal Extract only at high concentration (17.5 mg protein/kg sausage mixture) or the combined protease and Fungal Extract at low concentration (1.75 mg protein/kg sausage mixture), produced slight physi cochemical modifications compared ...
Fan Bicheng - One of the best experts on this subject based on the ideXlab platform.
-
Pyrenosetins A–C, new decalinoylspirotetramic acid derivatives isolated by bioactivity-based molecular networking from the seaweed-derived fungus Pyrenochaetopsis sp. FVE-001
'MDPI AG', 2020Co-Authors: Fan Bicheng, Dewapriya Pradeep, Li Fengjie, Blümel Martina, Tasdemir DenizAbstract:Marine algae represent a prolific source of filamentous fungi for bioprospecting. In continuation of our search for new anticancer leads from fungi derived from the brown alga , an endophytic sp. FVE-001 was selected for an in-depth chemical analysis. The crude Fungal Extract inhibited several cancer cell lines in vitro, and the highest anticancer activity was tracked to its CHCl-soluble portion. A bioactivity-based molecular networking approach was applied to C-SPE fractions of the CHCl subExtract to predict the bioactivity scores of metabolites in the fractions and to aid targeted purification of anticancer metabolites. This approach led to a rapid isolation of three new decalinoylspirotetramic acid derivatives, pyrenosetins A-C (-) and the known decalin tetramic acid phomasetin (). The structures of the compounds were elucidated by extensive NMR, HR-ESIMS, FT-IR spectroscopy, [α] and Mosher's ester method. Compounds and showed high anticancer activity against malignant melanoma cell line A-375 (IC values 2.8 and 6.3 μM, respectively), in line with the bioactivity predictions. This is the first study focusing on secondary metabolites of a marine-derived sp. and the second investigation performed on the member of the genus
-
Pyrenosetins A-C, New Decalinoylspirotetramic Acid Derivatives Isolated by Bioactivity-Based Molecular Networking from the Seaweed-Derived Fungus Pyrenochaetopsis sp. FVE-001
'MDPI AG', 2020Co-Authors: Fan Bicheng, Dewapriya Pradeep, Li Fengjie, Blümel Martina, Tasdemir DenizAbstract:Marine algae represent a prolific source of filamentous fungi for bioprospecting. In continuation of our search for new anticancer leads from fungi derived from the brown alga Fucus vesiculosus, an endophytic Pyrenochaetopsis sp. FVE-001 was selected for an in-depth chemical analysis. The crude Fungal Extract inhibited several cancer cell lines in vitro, and the highest anticancer activity was tracked to its CHCl3-soluble portion. A bioactivity-based molecular networking approach was applied to C18-SPE fractions of the CHCl3 subExtract to predict the bioactivity scores of metabolites in the fractions and to aid targeted purification of anticancer metabolites. This approach led to a rapid isolation of three new decalinoylspirotetramic acid derivatives, pyrenosetins A-C (1-3) and the known decalin tetramic acid phomasetin (4). The structures of the compounds were elucidated by extensive NMR, HR-ESIMS, FT-IR spectroscopy, [α]D and Mosher's ester method. Compounds 1 and 2 showed high anticancer activity against malignant melanoma cell line A-375 (IC50 values 2.8 and 6.3 μM, respectively), in line with the bioactivity predictions. This is the first study focusing on secondary metabolites of a marine-derived Pyrenochaetopsis sp. and the second investigation performed on the member of the genus Pyrenochaetopsis
-
Pyrenosetins A–C, New Decalinoylspirotetramic Acid Derivatives Isolated by Bioactivity-Based Molecular Networking from the Seaweed-Derived Fungus Pyrenochaetopsis sp. FVE-001
'MDPI AG', 2020Co-Authors: Fan Bicheng, Dewapriya Pradeep, Li Fengjie, Blümel Martina, Tasdemir DenizAbstract:Marine algae represent a prolific source of filamentous fungi for bioprospecting. In continuation of our search for new anticancer leads from fungi derived from the brown alga Fucus vesiculosus, an endophytic Pyrenochaetopsis sp. FVE-001 was selected for an in-depth chemical analysis. The crude Fungal Extract inhibited several cancer cell lines in vitro, and the highest anticancer activity was tracked to its CHCl3–soluble portion. A bioactivity-based molecular networking approach was applied to C18-SPE fractions of the CHCl3 subExtract to predict the bioactivity scores of metabolites in the fractions and to aid targeted purification of anticancer metabolites. This approach led to a rapid isolation of three new decalinoylspirotetramic acid derivatives, pyrenosetins A–C (1–3) and the known decalin tetramic acid phomasetin (4). The structures of the compounds were elucidated by extensive NMR, HR-ESIMS, FT-IR spectroscopy, [α]D and Mosher’s ester method. Compounds 1 and 2 showed high anticancer activity against malignant melanoma cell line A-375 (IC50 values 2.8 and 6.3 μM, respectively), in line with the bioactivity predictions. This is the first study focusing on secondary metabolites of a marine-derived Pyrenochaetopsis sp. and the second investigation performed on the member of the genus Pyrenochaetopsis
