The Experts below are selected from a list of 444 Experts worldwide ranked by ideXlab platform
Charlotte M. Mistretta - One of the best experts on this subject based on the ideXlab platform.
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the Fungiform papilla is a complex multimodal oral sensory organ
Current Opinion in Physiology, 2021Co-Authors: Charlotte M. Mistretta, Robert M BradleyAbstract:When solid or liquid stimuli contact the tongue tip during eating, the sensations of taste, touch and temperature are immediately evoked, and tongue function relies on these simultaneous multimodal responses. We focus on the Fungiform papilla of the anterior tongue as a complex organ for taste, tactile and thermal modalities, all via chorda tympani nerve innervation from the geniculate ganglion. Rather than a review, our aim is to revise the classic archetype of the Fungiform as predominantly a taste bud residence only and instead emphasize an amended concept of the papilla as a multimodal organ. Neurophysiological maps of Fungiform papillae in functional receptive fields demonstrate responses to chemical, stroking and cold lingual stimuli. Roles are predicted for elaborate extragemmal nerve endings in tactile and temperature sensations, and potential functions for keratinocytes in noncanonical sensory signaling. The Fungiform papilla is presented as a polymodal lingual organ, not solely a gustatory papilla.
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Inhibition of epithelial HH/GLI signaling leads to alterations in Fungiform papillae and epithelium and loss of taste buds.
2016Co-Authors: Alexandre N. Ermilov, Archana Kumari, Ariell M. Joiner, Marina A. Grachtchouk, Benjamin L. Allen, Andrzej A. Dlugosz, Charlotte M. MistrettaAbstract:A. Fungiform papilla and taste bud diagrams to illustrate cells types and location of HH pathway signaling elements. Canonical HH signaling is restricted to cells expressing both Gli1 and Ptch1 (Control papilla and Taste bud, left). Center and right panel diagrams illustrate cell/tissue areas (blue) of dominant-negative transgene expression in doxycycline-inducible K5GliR and epiGliR mouse models. B. Three types of Fungiform papilla and Taste Bud (FP/TB) used for quantifying effects of HH repression: I, Typical FP/TB: Typical papilla morphology with intact apical taste bud; II, Atypical FP/TB: Atypical, mis-shapen papilla morphology and taste bud remnant or cluster of distinctly staining cells; III, Atypical FP/No TB: Atypical papilla morphology with keratinized apical point and no discernable taste bud cells. C. H&E sections illustrate FP/TB morphology for Control and K5GliR and epiGliR mouse tongues at indicated time points after transgene induction. Scale bar applies to all micrographs except low power and insets. Low power image for epiGliR Control illustrates overall tongue morphology. Inset at Day 11 indicates disruption of Fungiform and filiform papillae but at Day 35 K5GliR, inset illustrates intact filiform. Graphs present quantification of TYPE I, II and III taste organs at increasing durations of transgene activation. TYPE I (Typical) FP/TB are substantially reduced (K5GliR) or eliminated (epiGliR) whereas TYPE III (Atypical/No TB) organs accumulate. Numbers of tongues at each time point are included in parentheses in graph legends. Full statistics for ANOVA are presented in S1A and S1B Fig.
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Separate and distinctive roles for Wnt5a in tongue, lingual tissue and taste papilla development
Developmental biology, 2011Co-Authors: Hong Xiang Liu, Ann S. Grosse, Ken Iwatsuki, Yuji Mishina, Deborah L. Gumucio, Charlotte M. MistrettaAbstract:Although canonical Wnt signaling is known to regulate taste papilla induction and numbers, roles for noncanonical Wnt pathways in tongue and taste papilla development have not been explored. With mutant mice and whole tongue organ cultures we demonstrate that Wnt5a protein and message are within anterior tongue mesenchyme across embryo stages from the initiation of tongue formation, through papilla placode appearance and taste papilla development. The Wnt5a mutant tongue is severely shortened, with an ankyloglossia, and lingual mesenchyme is disorganized. However, Fungiform papilla morphology, number and innervation are preserved, as is expression of the papilla marker, Shh. These data demonstrate that the genetic regulation for tongue size and shape can be separated from that directing lingual papilla development. Preserved number of papillae in a shortened tongue results in an increased density of Fungiform papillae in the mutant tongues. In tongue organ cultures, exogenous Wnt5a profoundly suppresses papilla formation and simultaneously decreases canonical Wnt signaling as measured by the TOPGAL reporter. These findings suggest that Wnt5a antagonizes canonical Wnt signaling to dictate papilla number and spacing. In all, distinctive roles for Wnt5a in tongue size, Fungiform papilla patterning and development are shown and a necessary balance between non-canonical and canonical Wnt paths in regulating tongue growth and Fungiform papillae is proposed in a model, through the Ror2 receptor.
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wnt5a in tongue and Fungiform papilla development
Annals of the New York Academy of Sciences, 2009Co-Authors: Hong Xiang Liu, Ann S. Grosse, Deborah L. Gumucio, Katherine D Walton, Daniel Saims, Charlotte M. MistrettaAbstract:Fungiform papillae are complex taste organs that develop in a pattern on anterior tongue in rodent embryos. Several intrinsic secreted molecules are important for papilla development and patterning, including sonic hedgehog, bone morphogenetic proteins, Noggin, epidermal growth factor, and WNTs. Recent data about roles of WNTs in regulation of tongue and Fungiform papilla development lead to new insights about the importance of tissue and timing contexts when studying the effects of morphogenetic proteins. WNT/beta-catenin signaling is required for formation of Fungiform papillae, but not for determining tongue size and shape. In contrast, WNT5a apparently is important for tongue outgrowth, but not papilla development. Preliminary data from WNT5a mutant mice separate genetic programs for papilla number from those for tongue shape and size.
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Fungiform papilla pattern egf regulates inter papilla lingual epithelium and decreases papilla number by means of pi3k akt mek erk and p38 mapk signaling
Developmental Dynamics, 2008Co-Authors: Hong Xiang Liu, Bradley S Henson, Yanqiu Zhou, Nisha J Dsilva, Charlotte M. MistrettaAbstract:Fungiform papillae are epithelial taste organs that form on the tongue, requiring differentiation of papillae and inter-papilla epithelium. We tested roles of epidermal growth factor (EGF) and the receptor EGFR in papilla development. Developmentally, EGF was localized within and between papillae whereas EGFR was progressively restricted to inter-papilla epithelium. In tongue cultures, EGF decreased papillae and increased cell proliferation in inter-papilla epithelium in a concentration-dependent manner, whereas EGFR inhibitor increased and fused papillae. EGF preincubation could over-ride disruption of Shh signaling that ordinarily would effect a doubling of Fungiform papillae. With EGF-induced activation of EGFR, we demonstrated phosphorylation in PI3K/Akt, MEK/ERK, and p38 MAPK pathways; with pathway inhibitors (LY294002, U0126, SB203580) the EGF-mediated decrease in papillae was reversed, and synergistic actions were shown. Thus, EGF/EGFR signaling by means of PI3K/Akt, MEK/ERK, and p38 MAPK contributes to epithelial cell proliferation between papillae; this biases against papilla differentiation and reduces numbers of papillae. Developmental Dynamics 237:2378–2393, 2008. © 2008 Wiley-Liss, Inc.
Sumiyuki Mii - One of the best experts on this subject based on the ideXlab platform.
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comparison of nestin expressing multipotent stem cells in the tongue Fungiform papilla and vibrissa hair follicle
Journal of Cellular Biochemistry, 2014Co-Authors: Yasuyuki Amoh, Kensei Katsuoka, Sumiyuki Mii, Robert M HoffmanAbstract:We have previously reported that hair follicles contain multipotent stem cells, which express nestin and participate in follicle growth at anagen as well as in the extension of the follicle sensory nerve. The nestin-driven green fluorescent protein (ND-GFP) transgenic mouse labels all nestin-expressing cells with GFP. The hair follicle nestin-GFP cells can differentiate into neurons, Schwann cells, and other cell types. In this study, we describe nestin-expressing multipotent stem cells in the Fungiform papilla in the tongue. The nestin-expressing multipotent stem cells in the Fungiform papilla are located around a peripheral sensory nerve immediately below the taste bud and co-express the neural crest cell marker p75NTR. The Fungiform papilla cells formed spheres in suspension culture in DMEM-F12 medium supplemented with basic fibroblast growth factor (bFGF). The spheres consisted of nestin-expressing cells that co-expressed the neural crest marker p75NTR and which developed expression of the stem cell marker CD34. P75NTR, CD34 and nestin co-expression suggested that nestin-expressing cells comprising the Fungiform papilla spheres were in a relatively undifferentiated state. The nestin-expressing cells of these spheres acquired the following markers: β III tubulin typical of nerve cells; GFAP typical of glial cells; K15 typical of keratinocytes; and smooth-muscle antigen (SMA), after transfer to RPMI 1640 medium with 10% fetal bovine serum (FBS), suggesting they differentiated into multiple cell types. The results of the current study indicate nestin-expressing Fungiform papilla cells and the nestin-expressing hair follicle stem cells have common features of cell morphology and ability to differentiate into multiple cell types, suggesting their remarkable similarity. J. Cell. Biochem. 115: 1070–1076, 2014. © 2013 Wiley Periodicals, Inc.
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comparison of nestin expressing multipotent stem cells in the tongue Fungiform papilla and vibrissa hair follicle
Journal of Cellular Biochemistry, 2014Co-Authors: Yasuyuki Amoh, Kensei Katsuoka, Sumiyuki Mii, Robert M HoffmanAbstract:We have previously reported that hair follicles contain multipotent stem cells, which express nestin and participate in follicle growth at anagen as well as in the extension of the follicle sensory nerve. The nestin-driven green fluorescent protein (ND-GFP) transgenic mouse labels all nestin-expressing cells with GFP. The hair follicle nestin-GFP cells can differentiate into neurons, Schwann cells, and other cell types. In this study, we describe nestin-expressing multipotent stem cells in the Fungiform papilla in the tongue. The nestin-expressing multipotent stem cells in the Fungiform papilla are located around a peripheral sensory nerve immediately below the taste bud and co-express the neural crest cell marker p75(NTR) . The Fungiform papilla cells formed spheres in suspension culture in DMEM-F12 medium supplemented with basic fibroblast growth factor (bFGF). The spheres consisted of nestin-expressing cells that co-expressed the neural crest marker p75(NTR) and which developed expression of the stem cell marker CD34. P75(NTR), CD34 and nestin co-expression suggested that nestin-expressing cells comprising the Fungiform papilla spheres were in a relatively undifferentiated state. The nestin-expressing cells of these spheres acquired the following markers: β III tubulin typical of nerve cells; GFAP typical of glial cells; K15 typical of keratinocytes; and smooth-muscle antigen (SMA), after transfer to RPMI 1640 medium with 10% fetal bovine serum (FBS), suggesting they differentiated into multiple cell types. The results of the current study indicate nestin-expressing Fungiform papilla cells and the nestin-expressing hair follicle stem cells have common features of cell morphology and ability to differentiate into multiple cell types, suggesting their remarkable similarity.
Robert M Hoffman - One of the best experts on this subject based on the ideXlab platform.
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comparison of nestin expressing multipotent stem cells in the tongue Fungiform papilla and vibrissa hair follicle
Journal of Cellular Biochemistry, 2014Co-Authors: Yasuyuki Amoh, Kensei Katsuoka, Sumiyuki Mii, Robert M HoffmanAbstract:We have previously reported that hair follicles contain multipotent stem cells, which express nestin and participate in follicle growth at anagen as well as in the extension of the follicle sensory nerve. The nestin-driven green fluorescent protein (ND-GFP) transgenic mouse labels all nestin-expressing cells with GFP. The hair follicle nestin-GFP cells can differentiate into neurons, Schwann cells, and other cell types. In this study, we describe nestin-expressing multipotent stem cells in the Fungiform papilla in the tongue. The nestin-expressing multipotent stem cells in the Fungiform papilla are located around a peripheral sensory nerve immediately below the taste bud and co-express the neural crest cell marker p75NTR. The Fungiform papilla cells formed spheres in suspension culture in DMEM-F12 medium supplemented with basic fibroblast growth factor (bFGF). The spheres consisted of nestin-expressing cells that co-expressed the neural crest marker p75NTR and which developed expression of the stem cell marker CD34. P75NTR, CD34 and nestin co-expression suggested that nestin-expressing cells comprising the Fungiform papilla spheres were in a relatively undifferentiated state. The nestin-expressing cells of these spheres acquired the following markers: β III tubulin typical of nerve cells; GFAP typical of glial cells; K15 typical of keratinocytes; and smooth-muscle antigen (SMA), after transfer to RPMI 1640 medium with 10% fetal bovine serum (FBS), suggesting they differentiated into multiple cell types. The results of the current study indicate nestin-expressing Fungiform papilla cells and the nestin-expressing hair follicle stem cells have common features of cell morphology and ability to differentiate into multiple cell types, suggesting their remarkable similarity. J. Cell. Biochem. 115: 1070–1076, 2014. © 2013 Wiley Periodicals, Inc.
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comparison of nestin expressing multipotent stem cells in the tongue Fungiform papilla and vibrissa hair follicle
Journal of Cellular Biochemistry, 2014Co-Authors: Yasuyuki Amoh, Kensei Katsuoka, Sumiyuki Mii, Robert M HoffmanAbstract:We have previously reported that hair follicles contain multipotent stem cells, which express nestin and participate in follicle growth at anagen as well as in the extension of the follicle sensory nerve. The nestin-driven green fluorescent protein (ND-GFP) transgenic mouse labels all nestin-expressing cells with GFP. The hair follicle nestin-GFP cells can differentiate into neurons, Schwann cells, and other cell types. In this study, we describe nestin-expressing multipotent stem cells in the Fungiform papilla in the tongue. The nestin-expressing multipotent stem cells in the Fungiform papilla are located around a peripheral sensory nerve immediately below the taste bud and co-express the neural crest cell marker p75(NTR) . The Fungiform papilla cells formed spheres in suspension culture in DMEM-F12 medium supplemented with basic fibroblast growth factor (bFGF). The spheres consisted of nestin-expressing cells that co-expressed the neural crest marker p75(NTR) and which developed expression of the stem cell marker CD34. P75(NTR), CD34 and nestin co-expression suggested that nestin-expressing cells comprising the Fungiform papilla spheres were in a relatively undifferentiated state. The nestin-expressing cells of these spheres acquired the following markers: β III tubulin typical of nerve cells; GFAP typical of glial cells; K15 typical of keratinocytes; and smooth-muscle antigen (SMA), after transfer to RPMI 1640 medium with 10% fetal bovine serum (FBS), suggesting they differentiated into multiple cell types. The results of the current study indicate nestin-expressing Fungiform papilla cells and the nestin-expressing hair follicle stem cells have common features of cell morphology and ability to differentiate into multiple cell types, suggesting their remarkable similarity.
Yasuyuki Amoh - One of the best experts on this subject based on the ideXlab platform.
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comparison of nestin expressing multipotent stem cells in the tongue Fungiform papilla and vibrissa hair follicle
Journal of Cellular Biochemistry, 2014Co-Authors: Yasuyuki Amoh, Kensei Katsuoka, Sumiyuki Mii, Robert M HoffmanAbstract:We have previously reported that hair follicles contain multipotent stem cells, which express nestin and participate in follicle growth at anagen as well as in the extension of the follicle sensory nerve. The nestin-driven green fluorescent protein (ND-GFP) transgenic mouse labels all nestin-expressing cells with GFP. The hair follicle nestin-GFP cells can differentiate into neurons, Schwann cells, and other cell types. In this study, we describe nestin-expressing multipotent stem cells in the Fungiform papilla in the tongue. The nestin-expressing multipotent stem cells in the Fungiform papilla are located around a peripheral sensory nerve immediately below the taste bud and co-express the neural crest cell marker p75NTR. The Fungiform papilla cells formed spheres in suspension culture in DMEM-F12 medium supplemented with basic fibroblast growth factor (bFGF). The spheres consisted of nestin-expressing cells that co-expressed the neural crest marker p75NTR and which developed expression of the stem cell marker CD34. P75NTR, CD34 and nestin co-expression suggested that nestin-expressing cells comprising the Fungiform papilla spheres were in a relatively undifferentiated state. The nestin-expressing cells of these spheres acquired the following markers: β III tubulin typical of nerve cells; GFAP typical of glial cells; K15 typical of keratinocytes; and smooth-muscle antigen (SMA), after transfer to RPMI 1640 medium with 10% fetal bovine serum (FBS), suggesting they differentiated into multiple cell types. The results of the current study indicate nestin-expressing Fungiform papilla cells and the nestin-expressing hair follicle stem cells have common features of cell morphology and ability to differentiate into multiple cell types, suggesting their remarkable similarity. J. Cell. Biochem. 115: 1070–1076, 2014. © 2013 Wiley Periodicals, Inc.
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comparison of nestin expressing multipotent stem cells in the tongue Fungiform papilla and vibrissa hair follicle
Journal of Cellular Biochemistry, 2014Co-Authors: Yasuyuki Amoh, Kensei Katsuoka, Sumiyuki Mii, Robert M HoffmanAbstract:We have previously reported that hair follicles contain multipotent stem cells, which express nestin and participate in follicle growth at anagen as well as in the extension of the follicle sensory nerve. The nestin-driven green fluorescent protein (ND-GFP) transgenic mouse labels all nestin-expressing cells with GFP. The hair follicle nestin-GFP cells can differentiate into neurons, Schwann cells, and other cell types. In this study, we describe nestin-expressing multipotent stem cells in the Fungiform papilla in the tongue. The nestin-expressing multipotent stem cells in the Fungiform papilla are located around a peripheral sensory nerve immediately below the taste bud and co-express the neural crest cell marker p75(NTR) . The Fungiform papilla cells formed spheres in suspension culture in DMEM-F12 medium supplemented with basic fibroblast growth factor (bFGF). The spheres consisted of nestin-expressing cells that co-expressed the neural crest marker p75(NTR) and which developed expression of the stem cell marker CD34. P75(NTR), CD34 and nestin co-expression suggested that nestin-expressing cells comprising the Fungiform papilla spheres were in a relatively undifferentiated state. The nestin-expressing cells of these spheres acquired the following markers: β III tubulin typical of nerve cells; GFAP typical of glial cells; K15 typical of keratinocytes; and smooth-muscle antigen (SMA), after transfer to RPMI 1640 medium with 10% fetal bovine serum (FBS), suggesting they differentiated into multiple cell types. The results of the current study indicate nestin-expressing Fungiform papilla cells and the nestin-expressing hair follicle stem cells have common features of cell morphology and ability to differentiate into multiple cell types, suggesting their remarkable similarity.
Kensei Katsuoka - One of the best experts on this subject based on the ideXlab platform.
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comparison of nestin expressing multipotent stem cells in the tongue Fungiform papilla and vibrissa hair follicle
Journal of Cellular Biochemistry, 2014Co-Authors: Yasuyuki Amoh, Kensei Katsuoka, Sumiyuki Mii, Robert M HoffmanAbstract:We have previously reported that hair follicles contain multipotent stem cells, which express nestin and participate in follicle growth at anagen as well as in the extension of the follicle sensory nerve. The nestin-driven green fluorescent protein (ND-GFP) transgenic mouse labels all nestin-expressing cells with GFP. The hair follicle nestin-GFP cells can differentiate into neurons, Schwann cells, and other cell types. In this study, we describe nestin-expressing multipotent stem cells in the Fungiform papilla in the tongue. The nestin-expressing multipotent stem cells in the Fungiform papilla are located around a peripheral sensory nerve immediately below the taste bud and co-express the neural crest cell marker p75NTR. The Fungiform papilla cells formed spheres in suspension culture in DMEM-F12 medium supplemented with basic fibroblast growth factor (bFGF). The spheres consisted of nestin-expressing cells that co-expressed the neural crest marker p75NTR and which developed expression of the stem cell marker CD34. P75NTR, CD34 and nestin co-expression suggested that nestin-expressing cells comprising the Fungiform papilla spheres were in a relatively undifferentiated state. The nestin-expressing cells of these spheres acquired the following markers: β III tubulin typical of nerve cells; GFAP typical of glial cells; K15 typical of keratinocytes; and smooth-muscle antigen (SMA), after transfer to RPMI 1640 medium with 10% fetal bovine serum (FBS), suggesting they differentiated into multiple cell types. The results of the current study indicate nestin-expressing Fungiform papilla cells and the nestin-expressing hair follicle stem cells have common features of cell morphology and ability to differentiate into multiple cell types, suggesting their remarkable similarity. J. Cell. Biochem. 115: 1070–1076, 2014. © 2013 Wiley Periodicals, Inc.
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comparison of nestin expressing multipotent stem cells in the tongue Fungiform papilla and vibrissa hair follicle
Journal of Cellular Biochemistry, 2014Co-Authors: Yasuyuki Amoh, Kensei Katsuoka, Sumiyuki Mii, Robert M HoffmanAbstract:We have previously reported that hair follicles contain multipotent stem cells, which express nestin and participate in follicle growth at anagen as well as in the extension of the follicle sensory nerve. The nestin-driven green fluorescent protein (ND-GFP) transgenic mouse labels all nestin-expressing cells with GFP. The hair follicle nestin-GFP cells can differentiate into neurons, Schwann cells, and other cell types. In this study, we describe nestin-expressing multipotent stem cells in the Fungiform papilla in the tongue. The nestin-expressing multipotent stem cells in the Fungiform papilla are located around a peripheral sensory nerve immediately below the taste bud and co-express the neural crest cell marker p75(NTR) . The Fungiform papilla cells formed spheres in suspension culture in DMEM-F12 medium supplemented with basic fibroblast growth factor (bFGF). The spheres consisted of nestin-expressing cells that co-expressed the neural crest marker p75(NTR) and which developed expression of the stem cell marker CD34. P75(NTR), CD34 and nestin co-expression suggested that nestin-expressing cells comprising the Fungiform papilla spheres were in a relatively undifferentiated state. The nestin-expressing cells of these spheres acquired the following markers: β III tubulin typical of nerve cells; GFAP typical of glial cells; K15 typical of keratinocytes; and smooth-muscle antigen (SMA), after transfer to RPMI 1640 medium with 10% fetal bovine serum (FBS), suggesting they differentiated into multiple cell types. The results of the current study indicate nestin-expressing Fungiform papilla cells and the nestin-expressing hair follicle stem cells have common features of cell morphology and ability to differentiate into multiple cell types, suggesting their remarkable similarity.
