The Experts below are selected from a list of 249 Experts worldwide ranked by ideXlab platform
Robert D. Stipanovic - One of the best experts on this subject based on the ideXlab platform.
-
Detoxification of Fusaric Acid by the Soil Microbe Mucor rouxii.
Journal of agricultural and food chemistry, 2017Co-Authors: Frankie K. Crutcher, Lorraine S. Puckhaber, Alois A. Bell, Jinggao Liu, Sara E. Duke, Robert D. Stipanovic, Robert L. NicholsAbstract:Fusarium oxysporum f. sp. vasinfectum race 4 (VCG0114), which causes root rot and wilt of cotton (Gossypium hirsutum and G. barbadense), has been identified recently for the first time in the western hemisphere in certain fields in the San Joaquin Valley of California. This pathotype produces copious quantities of the plant toxin Fusaric Acid (5-butyl-2-pyridinecarboxylic Acid) compared to other isolates of F. oxysporum f. sp. vasinfectum (Fov) that are indigenous to the United States. Fusaric Acid is toxic to cotton plants and may help the pathogen compete with other microbes in the soil. We found that a laboratory strain of the fungus Mucor rouxii converts Fusaric Acid into a newly identified compound, 8-hydroxyFusaric Acid. The latter compound is significantly less phytotoxic to cotton than the parent compound. On the basis of bioassays of hydroxylated analogues of Fusaric Acid, hydroxylation of the butyl side chain of Fusaric Acid may affect a general detoxification of Fusaric Acid. Genes that control...
-
Detoxification of Fusaric Acid by the Soil Microbe Mucor rouxii
2017Co-Authors: Frankie K. Crutcher, Lorraine S. Puckhaber, Alois A. Bell, Jinggao Liu, Sara E. Duke, Robert D. Stipanovic, Robert L. NicholsAbstract:Fusarium oxysporum f. sp. vasinfectum race 4 (VCG0114), which causes root rot and wilt of cotton (Gossypium hirsutum and G. barbadense), has been identified recently for the first time in the western hemisphere in certain fields in the San Joaquin Valley of California. This pathotype produces copious quantities of the plant toxin Fusaric Acid (5-butyl-2-pyridinecarboxylic Acid) compared to other isolates of F. oxysporum f. sp. vasinfectum (Fov) that are indigenous to the United States. Fusaric Acid is toxic to cotton plants and may help the pathogen compete with other microbes in the soil. We found that a laboratory strain of the fungus Mucor rouxii converts Fusaric Acid into a newly identified compound, 8-hydroxyFusaric Acid. The latter compound is significantly less phytotoxic to cotton than the parent compound. On the basis of bioassays of hydroxylated analogues of Fusaric Acid, hydroxylation of the butyl side chain of Fusaric Acid may affect a general detoxification of Fusaric Acid. Genes that control this hydroxylation may be useful in developing biocontrol agents to manage Fov
-
Nuclear magnetic resonance (NMR) studies on the biosynthesis of Fusaric Acid from Fusarium oxysporum f. sp. vasinfectum.
Journal of agricultural and food chemistry, 2011Co-Authors: Robert D. Stipanovic, Lorraine S. Puckhaber, Alois A. Bell, Jinggao Liu, Michael H. Wheeler, Howard J. WilliamsAbstract:Fusarium oxysporum is a fungal pathogen that attacks many important plants. Uniquely pathogenic strains of F. oxysporum f. sp. vasinfectum were inadvertently imported into the United States on live cottonseed for dairy cattle feed. These strains produce exceptionally high concentrations of the phytotoxin Fusaric Acid. Thus, Fusaric Acid may be a critical component in the pathogenicity of these biotypes. This study investigated the biosynthesis of Fusaric Acid using 13C-labeled substrates including [1,2-13C2]acetate as well as 13C- and 15N-labeled aspartate and [15N]glutamine. The incorporation of labeled substrates is consistent with the biosynthesis of Fusaric Acid from three acetate units at C5–C6, C7–C8, and C9–C10, with the remaining carbons being derived from aspartate via oxaloacetate and the TCA cycle; the oxaloacetate originates in part by transamination of aspartate, but most of the oxaloacetate is derived by deamination of aspartate to fumarate by aspartase. The nitrogen from glutamine is more r...
-
Phytotoxicity of Fusaric Acid and analogs to cotton.
Toxicon : official journal of the International Society on Toxinology, 2010Co-Authors: Robert D. Stipanovic, Lorraine S. Puckhaber, Jinggao Liu, Alois A. BellAbstract:We developed a cotton cotyledonary leaf bioassay to test the phytotoxicity of Fusaric Acid (5-butylpicolinic Acid), picolinic Acid and related analogs. The compounds were dissolved in aqueous Tween 80, and 20 μL of the test solution was placed at three positions on the leaf, and a needle was used to puncture the leaf through each drop; the results were evaluated after 48 h. In contrast to previous studies, we found the carboxylic Acid group is essential for phytotoxicity. Nicotinic Acid was considerably less phytotoxic than picolinic Acid and conversion of picolinic Acid to the amide or N-oxide decreased phytotoxicity. Increasing the alkyl chain length at the 5-position on picolinic Acid from two up to five carbons atoms increased phytotoxicity. Fusaric Acid methyl ester, the most phytotoxic compound tested, is a naturally occurring compound; as such it has potential as a herbicide in organic farming.
Robert L. Nichols - One of the best experts on this subject based on the ideXlab platform.
-
Detoxification of Fusaric Acid by the Soil Microbe Mucor rouxii.
Journal of agricultural and food chemistry, 2017Co-Authors: Frankie K. Crutcher, Lorraine S. Puckhaber, Alois A. Bell, Jinggao Liu, Sara E. Duke, Robert D. Stipanovic, Robert L. NicholsAbstract:Fusarium oxysporum f. sp. vasinfectum race 4 (VCG0114), which causes root rot and wilt of cotton (Gossypium hirsutum and G. barbadense), has been identified recently for the first time in the western hemisphere in certain fields in the San Joaquin Valley of California. This pathotype produces copious quantities of the plant toxin Fusaric Acid (5-butyl-2-pyridinecarboxylic Acid) compared to other isolates of F. oxysporum f. sp. vasinfectum (Fov) that are indigenous to the United States. Fusaric Acid is toxic to cotton plants and may help the pathogen compete with other microbes in the soil. We found that a laboratory strain of the fungus Mucor rouxii converts Fusaric Acid into a newly identified compound, 8-hydroxyFusaric Acid. The latter compound is significantly less phytotoxic to cotton than the parent compound. On the basis of bioassays of hydroxylated analogues of Fusaric Acid, hydroxylation of the butyl side chain of Fusaric Acid may affect a general detoxification of Fusaric Acid. Genes that control...
-
Detoxification of Fusaric Acid by the Soil Microbe Mucor rouxii
2017Co-Authors: Frankie K. Crutcher, Lorraine S. Puckhaber, Alois A. Bell, Jinggao Liu, Sara E. Duke, Robert D. Stipanovic, Robert L. NicholsAbstract:Fusarium oxysporum f. sp. vasinfectum race 4 (VCG0114), which causes root rot and wilt of cotton (Gossypium hirsutum and G. barbadense), has been identified recently for the first time in the western hemisphere in certain fields in the San Joaquin Valley of California. This pathotype produces copious quantities of the plant toxin Fusaric Acid (5-butyl-2-pyridinecarboxylic Acid) compared to other isolates of F. oxysporum f. sp. vasinfectum (Fov) that are indigenous to the United States. Fusaric Acid is toxic to cotton plants and may help the pathogen compete with other microbes in the soil. We found that a laboratory strain of the fungus Mucor rouxii converts Fusaric Acid into a newly identified compound, 8-hydroxyFusaric Acid. The latter compound is significantly less phytotoxic to cotton than the parent compound. On the basis of bioassays of hydroxylated analogues of Fusaric Acid, hydroxylation of the butyl side chain of Fusaric Acid may affect a general detoxification of Fusaric Acid. Genes that control this hydroxylation may be useful in developing biocontrol agents to manage Fov
Lorraine S. Puckhaber - One of the best experts on this subject based on the ideXlab platform.
-
Detoxification of Fusaric Acid by the Soil Microbe Mucor rouxii.
Journal of agricultural and food chemistry, 2017Co-Authors: Frankie K. Crutcher, Lorraine S. Puckhaber, Alois A. Bell, Jinggao Liu, Sara E. Duke, Robert D. Stipanovic, Robert L. NicholsAbstract:Fusarium oxysporum f. sp. vasinfectum race 4 (VCG0114), which causes root rot and wilt of cotton (Gossypium hirsutum and G. barbadense), has been identified recently for the first time in the western hemisphere in certain fields in the San Joaquin Valley of California. This pathotype produces copious quantities of the plant toxin Fusaric Acid (5-butyl-2-pyridinecarboxylic Acid) compared to other isolates of F. oxysporum f. sp. vasinfectum (Fov) that are indigenous to the United States. Fusaric Acid is toxic to cotton plants and may help the pathogen compete with other microbes in the soil. We found that a laboratory strain of the fungus Mucor rouxii converts Fusaric Acid into a newly identified compound, 8-hydroxyFusaric Acid. The latter compound is significantly less phytotoxic to cotton than the parent compound. On the basis of bioassays of hydroxylated analogues of Fusaric Acid, hydroxylation of the butyl side chain of Fusaric Acid may affect a general detoxification of Fusaric Acid. Genes that control...
-
Detoxification of Fusaric Acid by the Soil Microbe Mucor rouxii
2017Co-Authors: Frankie K. Crutcher, Lorraine S. Puckhaber, Alois A. Bell, Jinggao Liu, Sara E. Duke, Robert D. Stipanovic, Robert L. NicholsAbstract:Fusarium oxysporum f. sp. vasinfectum race 4 (VCG0114), which causes root rot and wilt of cotton (Gossypium hirsutum and G. barbadense), has been identified recently for the first time in the western hemisphere in certain fields in the San Joaquin Valley of California. This pathotype produces copious quantities of the plant toxin Fusaric Acid (5-butyl-2-pyridinecarboxylic Acid) compared to other isolates of F. oxysporum f. sp. vasinfectum (Fov) that are indigenous to the United States. Fusaric Acid is toxic to cotton plants and may help the pathogen compete with other microbes in the soil. We found that a laboratory strain of the fungus Mucor rouxii converts Fusaric Acid into a newly identified compound, 8-hydroxyFusaric Acid. The latter compound is significantly less phytotoxic to cotton than the parent compound. On the basis of bioassays of hydroxylated analogues of Fusaric Acid, hydroxylation of the butyl side chain of Fusaric Acid may affect a general detoxification of Fusaric Acid. Genes that control this hydroxylation may be useful in developing biocontrol agents to manage Fov
-
Nuclear magnetic resonance (NMR) studies on the biosynthesis of Fusaric Acid from Fusarium oxysporum f. sp. vasinfectum.
Journal of agricultural and food chemistry, 2011Co-Authors: Robert D. Stipanovic, Lorraine S. Puckhaber, Alois A. Bell, Jinggao Liu, Michael H. Wheeler, Howard J. WilliamsAbstract:Fusarium oxysporum is a fungal pathogen that attacks many important plants. Uniquely pathogenic strains of F. oxysporum f. sp. vasinfectum were inadvertently imported into the United States on live cottonseed for dairy cattle feed. These strains produce exceptionally high concentrations of the phytotoxin Fusaric Acid. Thus, Fusaric Acid may be a critical component in the pathogenicity of these biotypes. This study investigated the biosynthesis of Fusaric Acid using 13C-labeled substrates including [1,2-13C2]acetate as well as 13C- and 15N-labeled aspartate and [15N]glutamine. The incorporation of labeled substrates is consistent with the biosynthesis of Fusaric Acid from three acetate units at C5–C6, C7–C8, and C9–C10, with the remaining carbons being derived from aspartate via oxaloacetate and the TCA cycle; the oxaloacetate originates in part by transamination of aspartate, but most of the oxaloacetate is derived by deamination of aspartate to fumarate by aspartase. The nitrogen from glutamine is more r...
-
Phytotoxicity of Fusaric Acid and analogs to cotton.
Toxicon : official journal of the International Society on Toxinology, 2010Co-Authors: Robert D. Stipanovic, Lorraine S. Puckhaber, Jinggao Liu, Alois A. BellAbstract:We developed a cotton cotyledonary leaf bioassay to test the phytotoxicity of Fusaric Acid (5-butylpicolinic Acid), picolinic Acid and related analogs. The compounds were dissolved in aqueous Tween 80, and 20 μL of the test solution was placed at three positions on the leaf, and a needle was used to puncture the leaf through each drop; the results were evaluated after 48 h. In contrast to previous studies, we found the carboxylic Acid group is essential for phytotoxicity. Nicotinic Acid was considerably less phytotoxic than picolinic Acid and conversion of picolinic Acid to the amide or N-oxide decreased phytotoxicity. Increasing the alkyl chain length at the 5-position on picolinic Acid from two up to five carbons atoms increased phytotoxicity. Fusaric Acid methyl ester, the most phytotoxic compound tested, is a naturally occurring compound; as such it has potential as a herbicide in organic farming.
Jinggao Liu - One of the best experts on this subject based on the ideXlab platform.
-
Detoxification of Fusaric Acid by the Soil Microbe Mucor rouxii.
Journal of agricultural and food chemistry, 2017Co-Authors: Frankie K. Crutcher, Lorraine S. Puckhaber, Alois A. Bell, Jinggao Liu, Sara E. Duke, Robert D. Stipanovic, Robert L. NicholsAbstract:Fusarium oxysporum f. sp. vasinfectum race 4 (VCG0114), which causes root rot and wilt of cotton (Gossypium hirsutum and G. barbadense), has been identified recently for the first time in the western hemisphere in certain fields in the San Joaquin Valley of California. This pathotype produces copious quantities of the plant toxin Fusaric Acid (5-butyl-2-pyridinecarboxylic Acid) compared to other isolates of F. oxysporum f. sp. vasinfectum (Fov) that are indigenous to the United States. Fusaric Acid is toxic to cotton plants and may help the pathogen compete with other microbes in the soil. We found that a laboratory strain of the fungus Mucor rouxii converts Fusaric Acid into a newly identified compound, 8-hydroxyFusaric Acid. The latter compound is significantly less phytotoxic to cotton than the parent compound. On the basis of bioassays of hydroxylated analogues of Fusaric Acid, hydroxylation of the butyl side chain of Fusaric Acid may affect a general detoxification of Fusaric Acid. Genes that control...
-
Detoxification of Fusaric Acid by the Soil Microbe Mucor rouxii
2017Co-Authors: Frankie K. Crutcher, Lorraine S. Puckhaber, Alois A. Bell, Jinggao Liu, Sara E. Duke, Robert D. Stipanovic, Robert L. NicholsAbstract:Fusarium oxysporum f. sp. vasinfectum race 4 (VCG0114), which causes root rot and wilt of cotton (Gossypium hirsutum and G. barbadense), has been identified recently for the first time in the western hemisphere in certain fields in the San Joaquin Valley of California. This pathotype produces copious quantities of the plant toxin Fusaric Acid (5-butyl-2-pyridinecarboxylic Acid) compared to other isolates of F. oxysporum f. sp. vasinfectum (Fov) that are indigenous to the United States. Fusaric Acid is toxic to cotton plants and may help the pathogen compete with other microbes in the soil. We found that a laboratory strain of the fungus Mucor rouxii converts Fusaric Acid into a newly identified compound, 8-hydroxyFusaric Acid. The latter compound is significantly less phytotoxic to cotton than the parent compound. On the basis of bioassays of hydroxylated analogues of Fusaric Acid, hydroxylation of the butyl side chain of Fusaric Acid may affect a general detoxification of Fusaric Acid. Genes that control this hydroxylation may be useful in developing biocontrol agents to manage Fov
-
Nuclear magnetic resonance (NMR) studies on the biosynthesis of Fusaric Acid from Fusarium oxysporum f. sp. vasinfectum.
Journal of agricultural and food chemistry, 2011Co-Authors: Robert D. Stipanovic, Lorraine S. Puckhaber, Alois A. Bell, Jinggao Liu, Michael H. Wheeler, Howard J. WilliamsAbstract:Fusarium oxysporum is a fungal pathogen that attacks many important plants. Uniquely pathogenic strains of F. oxysporum f. sp. vasinfectum were inadvertently imported into the United States on live cottonseed for dairy cattle feed. These strains produce exceptionally high concentrations of the phytotoxin Fusaric Acid. Thus, Fusaric Acid may be a critical component in the pathogenicity of these biotypes. This study investigated the biosynthesis of Fusaric Acid using 13C-labeled substrates including [1,2-13C2]acetate as well as 13C- and 15N-labeled aspartate and [15N]glutamine. The incorporation of labeled substrates is consistent with the biosynthesis of Fusaric Acid from three acetate units at C5–C6, C7–C8, and C9–C10, with the remaining carbons being derived from aspartate via oxaloacetate and the TCA cycle; the oxaloacetate originates in part by transamination of aspartate, but most of the oxaloacetate is derived by deamination of aspartate to fumarate by aspartase. The nitrogen from glutamine is more r...
-
Phytotoxicity of Fusaric Acid and analogs to cotton.
Toxicon : official journal of the International Society on Toxinology, 2010Co-Authors: Robert D. Stipanovic, Lorraine S. Puckhaber, Jinggao Liu, Alois A. BellAbstract:We developed a cotton cotyledonary leaf bioassay to test the phytotoxicity of Fusaric Acid (5-butylpicolinic Acid), picolinic Acid and related analogs. The compounds were dissolved in aqueous Tween 80, and 20 μL of the test solution was placed at three positions on the leaf, and a needle was used to puncture the leaf through each drop; the results were evaluated after 48 h. In contrast to previous studies, we found the carboxylic Acid group is essential for phytotoxicity. Nicotinic Acid was considerably less phytotoxic than picolinic Acid and conversion of picolinic Acid to the amide or N-oxide decreased phytotoxicity. Increasing the alkyl chain length at the 5-position on picolinic Acid from two up to five carbons atoms increased phytotoxicity. Fusaric Acid methyl ester, the most phytotoxic compound tested, is a naturally occurring compound; as such it has potential as a herbicide in organic farming.
Alois A. Bell - One of the best experts on this subject based on the ideXlab platform.
-
Detoxification of Fusaric Acid by the Soil Microbe Mucor rouxii.
Journal of agricultural and food chemistry, 2017Co-Authors: Frankie K. Crutcher, Lorraine S. Puckhaber, Alois A. Bell, Jinggao Liu, Sara E. Duke, Robert D. Stipanovic, Robert L. NicholsAbstract:Fusarium oxysporum f. sp. vasinfectum race 4 (VCG0114), which causes root rot and wilt of cotton (Gossypium hirsutum and G. barbadense), has been identified recently for the first time in the western hemisphere in certain fields in the San Joaquin Valley of California. This pathotype produces copious quantities of the plant toxin Fusaric Acid (5-butyl-2-pyridinecarboxylic Acid) compared to other isolates of F. oxysporum f. sp. vasinfectum (Fov) that are indigenous to the United States. Fusaric Acid is toxic to cotton plants and may help the pathogen compete with other microbes in the soil. We found that a laboratory strain of the fungus Mucor rouxii converts Fusaric Acid into a newly identified compound, 8-hydroxyFusaric Acid. The latter compound is significantly less phytotoxic to cotton than the parent compound. On the basis of bioassays of hydroxylated analogues of Fusaric Acid, hydroxylation of the butyl side chain of Fusaric Acid may affect a general detoxification of Fusaric Acid. Genes that control...
-
Detoxification of Fusaric Acid by the Soil Microbe Mucor rouxii
2017Co-Authors: Frankie K. Crutcher, Lorraine S. Puckhaber, Alois A. Bell, Jinggao Liu, Sara E. Duke, Robert D. Stipanovic, Robert L. NicholsAbstract:Fusarium oxysporum f. sp. vasinfectum race 4 (VCG0114), which causes root rot and wilt of cotton (Gossypium hirsutum and G. barbadense), has been identified recently for the first time in the western hemisphere in certain fields in the San Joaquin Valley of California. This pathotype produces copious quantities of the plant toxin Fusaric Acid (5-butyl-2-pyridinecarboxylic Acid) compared to other isolates of F. oxysporum f. sp. vasinfectum (Fov) that are indigenous to the United States. Fusaric Acid is toxic to cotton plants and may help the pathogen compete with other microbes in the soil. We found that a laboratory strain of the fungus Mucor rouxii converts Fusaric Acid into a newly identified compound, 8-hydroxyFusaric Acid. The latter compound is significantly less phytotoxic to cotton than the parent compound. On the basis of bioassays of hydroxylated analogues of Fusaric Acid, hydroxylation of the butyl side chain of Fusaric Acid may affect a general detoxification of Fusaric Acid. Genes that control this hydroxylation may be useful in developing biocontrol agents to manage Fov
-
Nuclear magnetic resonance (NMR) studies on the biosynthesis of Fusaric Acid from Fusarium oxysporum f. sp. vasinfectum.
Journal of agricultural and food chemistry, 2011Co-Authors: Robert D. Stipanovic, Lorraine S. Puckhaber, Alois A. Bell, Jinggao Liu, Michael H. Wheeler, Howard J. WilliamsAbstract:Fusarium oxysporum is a fungal pathogen that attacks many important plants. Uniquely pathogenic strains of F. oxysporum f. sp. vasinfectum were inadvertently imported into the United States on live cottonseed for dairy cattle feed. These strains produce exceptionally high concentrations of the phytotoxin Fusaric Acid. Thus, Fusaric Acid may be a critical component in the pathogenicity of these biotypes. This study investigated the biosynthesis of Fusaric Acid using 13C-labeled substrates including [1,2-13C2]acetate as well as 13C- and 15N-labeled aspartate and [15N]glutamine. The incorporation of labeled substrates is consistent with the biosynthesis of Fusaric Acid from three acetate units at C5–C6, C7–C8, and C9–C10, with the remaining carbons being derived from aspartate via oxaloacetate and the TCA cycle; the oxaloacetate originates in part by transamination of aspartate, but most of the oxaloacetate is derived by deamination of aspartate to fumarate by aspartase. The nitrogen from glutamine is more r...
-
Phytotoxicity of Fusaric Acid and analogs to cotton.
Toxicon : official journal of the International Society on Toxinology, 2010Co-Authors: Robert D. Stipanovic, Lorraine S. Puckhaber, Jinggao Liu, Alois A. BellAbstract:We developed a cotton cotyledonary leaf bioassay to test the phytotoxicity of Fusaric Acid (5-butylpicolinic Acid), picolinic Acid and related analogs. The compounds were dissolved in aqueous Tween 80, and 20 μL of the test solution was placed at three positions on the leaf, and a needle was used to puncture the leaf through each drop; the results were evaluated after 48 h. In contrast to previous studies, we found the carboxylic Acid group is essential for phytotoxicity. Nicotinic Acid was considerably less phytotoxic than picolinic Acid and conversion of picolinic Acid to the amide or N-oxide decreased phytotoxicity. Increasing the alkyl chain length at the 5-position on picolinic Acid from two up to five carbons atoms increased phytotoxicity. Fusaric Acid methyl ester, the most phytotoxic compound tested, is a naturally occurring compound; as such it has potential as a herbicide in organic farming.
