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Kim Hammond-kosack - One of the best experts on this subject based on the ideXlab platform.
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Inter-genome comparison of the Quorn fungus Fusarium venenatum and the closely related plant infecting pathogen Fusarium graminearum
BMC Genomics, 2018Co-Authors: Robert King, Martin Urban, Neil Andrew Brown, Kim Hammond-kosackAbstract:Background The soil dwelling saprotrophic non-pathogenic fungus Fusarium venenatum , routinely used in the commercial fermentation industry, is phylogenetically closely related to the globally important cereal and non-cereal infecting pathogen F. graminearum . This study aimed to sequence, assemble and annotate the F. venenatum (strain A3/5) genome, and compare this genome with F. graminearum . Results Using shotgun sequencing, a 38,660,329 bp F. venenatum genome was assembled into four chromosomes, and a 78,618 bp mitochondrial genome. In comparison to F. graminearum , the predicted gene count of 13,946 was slightly lower. The F. venenatum centromeres were found to be 25% smaller compared to F. graminearum . Chromosome length was 2.8% greater in F. venenatum, primarily due to an increased abundance of repetitive elements and transposons, but not transposon diversity. On chromosome 3 a major sequence rearrangement was found, but its overall gene content was relatively unchanged. Unlike homothallic F. graminearum , heterothallic F. venenatum possessed the MAT1–1 type locus, but lacked the MAT1–2 locus. The F. venenatum genome has the type A trichothecene mycotoxin TRI5 cluster, whereas F. graminearum has type B. From the F. venenatum gene set, 786 predicted proteins were species-specific versus NCBI. The annotated F. venenatum genome was predicted to possess more genes coding for hydrolytic enzymes and species - specific genes involved in the breakdown of polysaccharides than F. graminearum . Comparison of the two genomes reduced the previously defined F. graminearum- specific gene set from 741 to 692 genes. A comparison of the F. graminearum versus F. venenatum proteomes identified 15 putative secondary metabolite gene clusters (SMC), 109 secreted proteins and 38 candidate effectors not found in F. venenatum . Five of the 15 F. graminearum-specific SMCs that were either absent or highly divergent in the F. venenatum genome showed increased in planta expression. In addition, two predicted F. graminearum transcription factors previously shown to be required for fungal virulence on wheat plants were absent or exhibited high sequence divergence. Conclusions This study identifies differences between the F. venenatum and F. graminearum genomes that may contribute to contrasting lifestyles, and highlights the repertoire of F. graminearum -specific candidate genes and SMCs potentially required for pathogenesis.
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Additional file 11: of Inter-genome comparison of the Quorn fungus Fusarium venenatum and the closely related plant infecting pathogen Fusarium graminearum
2018Co-Authors: Robert King, Neil Brown, Martin Urban, Kim Hammond-kosackAbstract:The mating locus within the F. venenatum and F. graminearum genomes. F. venenatum A3/5 contains only the MAT1–1 locus confirming heterothallism and the requirement for a complementary sexual partner. Whilst homothallic F. graminearum possesses both the MAT1–1 and MAT1–2 loci and hence does not require a complementary partner for sexual reproduction. Gene designations: A) FGRRES_08887, B) FGRRES_08888_M, C) FGRRES_08889_M, D) FGRRES_08894, E) FVRRES_05564, F) FGRRES_15525, G) FGRRES_13273_M. (PDF 90 kb
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Additional file 17: of Inter-genome comparison of the Quorn fungus Fusarium venenatum and the closely related plant infecting pathogen Fusarium graminearum
2018Co-Authors: Robert King, Neil Brown, Martin Urban, Kim Hammond-kosackAbstract:Fusarium venenatum presence of TRI6 Fusarium greaminearum binding sites predicted by Nasmith et al. [39]. Fusarium venenatum BLASTP alignment percentages were added to identify presence or absence. (XLS 61Â kb
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Additional file 9: of Inter-genome comparison of the Quorn fungus Fusarium venenatum and the closely related plant infecting pathogen Fusarium graminearum
2018Co-Authors: Robert King, Neil Brown, Martin Urban, Kim Hammond-kosackAbstract:Fusarium graminearum alignment and gene IDâs found in the inverted/translocated regions A/B/C in relation to Fusarium venenatum. Mauve alignments in Sheet 1 of Fusarium venenatum and F. graminearum chromosome 3. The small blue inversion/translocation in Fusarium graminearum has 56 genes, the small green translocation 144 genes, and the large blue/pink inversion/translocation has 586 genes (see Sheet 2â4). Below the top mauve alignment are F. venenatum and F. poae chromosome 3 aligned to F. graminearum using Lastz. (XLS 678Â kb
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Additional file 10: of Inter-genome comparison of the Quorn fungus Fusarium venenatum and the closely related plant infecting pathogen Fusarium graminearum
2018Co-Authors: Robert King, Neil Brown, Martin Urban, Kim Hammond-kosackAbstract:Classes of transposon and repeat found in Fusarium graminearum and Fusarium venenatum. (XLS 37Â kb
Robert King - One of the best experts on this subject based on the ideXlab platform.
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Inter-genome comparison of the Quorn fungus Fusarium venenatum and the closely related plant infecting pathogen Fusarium graminearum
BMC Genomics, 2018Co-Authors: Robert King, Martin Urban, Neil Andrew Brown, Kim Hammond-kosackAbstract:Background The soil dwelling saprotrophic non-pathogenic fungus Fusarium venenatum , routinely used in the commercial fermentation industry, is phylogenetically closely related to the globally important cereal and non-cereal infecting pathogen F. graminearum . This study aimed to sequence, assemble and annotate the F. venenatum (strain A3/5) genome, and compare this genome with F. graminearum . Results Using shotgun sequencing, a 38,660,329 bp F. venenatum genome was assembled into four chromosomes, and a 78,618 bp mitochondrial genome. In comparison to F. graminearum , the predicted gene count of 13,946 was slightly lower. The F. venenatum centromeres were found to be 25% smaller compared to F. graminearum . Chromosome length was 2.8% greater in F. venenatum, primarily due to an increased abundance of repetitive elements and transposons, but not transposon diversity. On chromosome 3 a major sequence rearrangement was found, but its overall gene content was relatively unchanged. Unlike homothallic F. graminearum , heterothallic F. venenatum possessed the MAT1–1 type locus, but lacked the MAT1–2 locus. The F. venenatum genome has the type A trichothecene mycotoxin TRI5 cluster, whereas F. graminearum has type B. From the F. venenatum gene set, 786 predicted proteins were species-specific versus NCBI. The annotated F. venenatum genome was predicted to possess more genes coding for hydrolytic enzymes and species - specific genes involved in the breakdown of polysaccharides than F. graminearum . Comparison of the two genomes reduced the previously defined F. graminearum- specific gene set from 741 to 692 genes. A comparison of the F. graminearum versus F. venenatum proteomes identified 15 putative secondary metabolite gene clusters (SMC), 109 secreted proteins and 38 candidate effectors not found in F. venenatum . Five of the 15 F. graminearum-specific SMCs that were either absent or highly divergent in the F. venenatum genome showed increased in planta expression. In addition, two predicted F. graminearum transcription factors previously shown to be required for fungal virulence on wheat plants were absent or exhibited high sequence divergence. Conclusions This study identifies differences between the F. venenatum and F. graminearum genomes that may contribute to contrasting lifestyles, and highlights the repertoire of F. graminearum -specific candidate genes and SMCs potentially required for pathogenesis.
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Additional file 11: of Inter-genome comparison of the Quorn fungus Fusarium venenatum and the closely related plant infecting pathogen Fusarium graminearum
2018Co-Authors: Robert King, Neil Brown, Martin Urban, Kim Hammond-kosackAbstract:The mating locus within the F. venenatum and F. graminearum genomes. F. venenatum A3/5 contains only the MAT1–1 locus confirming heterothallism and the requirement for a complementary sexual partner. Whilst homothallic F. graminearum possesses both the MAT1–1 and MAT1–2 loci and hence does not require a complementary partner for sexual reproduction. Gene designations: A) FGRRES_08887, B) FGRRES_08888_M, C) FGRRES_08889_M, D) FGRRES_08894, E) FVRRES_05564, F) FGRRES_15525, G) FGRRES_13273_M. (PDF 90 kb
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Additional file 17: of Inter-genome comparison of the Quorn fungus Fusarium venenatum and the closely related plant infecting pathogen Fusarium graminearum
2018Co-Authors: Robert King, Neil Brown, Martin Urban, Kim Hammond-kosackAbstract:Fusarium venenatum presence of TRI6 Fusarium greaminearum binding sites predicted by Nasmith et al. [39]. Fusarium venenatum BLASTP alignment percentages were added to identify presence or absence. (XLS 61Â kb
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Additional file 9: of Inter-genome comparison of the Quorn fungus Fusarium venenatum and the closely related plant infecting pathogen Fusarium graminearum
2018Co-Authors: Robert King, Neil Brown, Martin Urban, Kim Hammond-kosackAbstract:Fusarium graminearum alignment and gene IDâs found in the inverted/translocated regions A/B/C in relation to Fusarium venenatum. Mauve alignments in Sheet 1 of Fusarium venenatum and F. graminearum chromosome 3. The small blue inversion/translocation in Fusarium graminearum has 56 genes, the small green translocation 144 genes, and the large blue/pink inversion/translocation has 586 genes (see Sheet 2â4). Below the top mauve alignment are F. venenatum and F. poae chromosome 3 aligned to F. graminearum using Lastz. (XLS 678Â kb
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Additional file 10: of Inter-genome comparison of the Quorn fungus Fusarium venenatum and the closely related plant infecting pathogen Fusarium graminearum
2018Co-Authors: Robert King, Neil Brown, Martin Urban, Kim Hammond-kosackAbstract:Classes of transposon and repeat found in Fusarium graminearum and Fusarium venenatum. (XLS 37Â kb
Brunello Wuethrich - One of the best experts on this subject based on the ideXlab platform.
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immediate type hypersensitivity reaction to ingestion of mycoprotein quorn in a patient allergic to molds caused by acidic ribosomal protein p2
The Journal of Allergy and Clinical Immunology, 2003Co-Authors: Michael Hoff, Ralph M Trueb, Barbara Ballmerweber, Stefan Vieths, Brunello WuethrichAbstract:BACKGROUND: Quorn is the brand name for a line of foods made with so-called "mycoprotein," which springs from the mold Fusarium venenatum. Since the introduction on the food market, there have been complaints from consumers reporting adverse gastrointestinal reactions after ingestion of mycoprotein. To date, it is not clear whether the reported symptoms are IgE-mediated. OBJECTIVE: The aim of the study was to describe for the first time a case history of an asthmatic patient with severe hypersensitivity reactions to ingested mycoprotein and to identify and characterize the potential allergen that might be responsible for this. METHODS: The sensitization pattern of the asthmatic subject was characterized, and food allergy to mycoprotein was assessed by double-blinded placebo-controlled food challenge. Afterward, specific IgE antibodies of the serum of this patient were used to screen a Fusarium culmorum cDNA expression library. The coding sequence of one enriched cDNA-clone was expressed in Escherichia coli to produce a recombinant protein that was further purified and immunologically characterized. RESULTS: The patient showed high sensitization to many known aeroallergens but apart from Quorn not to any other tested food samples. The deduced amino acid sequence of the enriched cDNA-clone (Fus c 1) showed large identity to the 60S acidic ribosomal protein P2 which is highly conserved among several species and also described as minor allergen in other mold species. The frequency of IgE reactivity of sera from F culmorum -sensitized subjects to rFus c 1 was approximately 35%. By enzyme allergosorbent test inhibition, we found 65% inhibition of mycoprotein IgE reactivity by rFus c 1. On the opposite we found reduced IgE reactivity of rFus c 1 of 68% by using mycoprotein as inhibitor. CONCLUSIONS: Sensitization to mold allergens by the respiratory tract and subsequent oral ingestion of cross-reactive proteins may lead to severe food-allergic reactions. Thus, the 60S acidic ribosomal protein P2 of F venenatum probably is the reason for the described severe hypersensitivity reactions of the patient to Quorn-mycoprotein because of its potential cross-reactivity to the F culmorum allergen Fus c 1.
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Immediate-type hypersensitivity reaction to ingestion of mycoprotein (Quorn) in a patient allergic to molds caused by acidic ribosomal protein P2.
Journal of Allergy and Clinical Immunology, 2003Co-Authors: Michael Hoff, Ralph M Trueb, Stefan Vieths, Barbara Ballmer-weber, Brunello WuethrichAbstract:Abstract Background: Quorn is the brand name for a line of foods made with so-called "mycoprotein," which springs from the mold Fusarium venenatum . Since the introduction on the food market, there have been complaints from consumers reporting adverse gastrointestinal reactions after ingestion of mycoprotein. To date, it is not clear whether the reported symptoms are IgE-mediated. Objective: The aim of the study was to describe for the first time a case history of an asthmatic patient with severe hypersensitivity reactions to ingested mycoprotein and to identify and characterize the potential allergen that might be responsible for this. Methods: The sensitization pattern of the asthmatic subject was characterized, and food allergy to mycoprotein was assessed by double-blinded placebo-controlled food challenge. Afterward, specific IgE antibodies of the serum of this patient were used to screen a Fusarium culmorum cDNA expression library. The coding sequence of one enriched cDNA-clone was expressed in Escherichia coli to produce a recombinant protein that was further purified and immunologically characterized. Results: The patient showed high sensitization to many known aeroallergens but apart from Quorn not to any other tested food samples. The deduced amino acid sequence of the enriched cDNA-clone (Fus c 1) showed large identity to the 60S acidic ribosomal protein P2 which is highly conserved among several species and also described as minor allergen in other mold species. The frequency of IgE reactivity of sera from F culmorum -sensitized subjects to rFus c 1 was approximately 35%. By enzyme allergosorbent test inhibition, we found 65% inhibition of mycoprotein IgE reactivity by rFus c 1 . On the opposite we found reduced IgE reactivity of rFus c 1 of 68% by using mycoprotein as inhibitor. Conclusions: Sensitization to mold allergens by the respiratory tract and subsequent oral ingestion of cross-reactive proteins may lead to severe food-allergic reactions. Thus, the 60S acidic ribosomal protein P2 of F venenatum probably is the reason for the described severe hypersensitivity reactions of the patient to Quorn-mycoprotein because of its potential cross-reactivity to the F culmorum allergen Fus c 1. (J Allergy Clin Immunol 2003;111:1106-10.)
Martin Urban - One of the best experts on this subject based on the ideXlab platform.
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Inter-genome comparison of the Quorn fungus Fusarium venenatum and the closely related plant infecting pathogen Fusarium graminearum
BMC Genomics, 2018Co-Authors: Robert King, Martin Urban, Neil Andrew Brown, Kim Hammond-kosackAbstract:Background The soil dwelling saprotrophic non-pathogenic fungus Fusarium venenatum , routinely used in the commercial fermentation industry, is phylogenetically closely related to the globally important cereal and non-cereal infecting pathogen F. graminearum . This study aimed to sequence, assemble and annotate the F. venenatum (strain A3/5) genome, and compare this genome with F. graminearum . Results Using shotgun sequencing, a 38,660,329 bp F. venenatum genome was assembled into four chromosomes, and a 78,618 bp mitochondrial genome. In comparison to F. graminearum , the predicted gene count of 13,946 was slightly lower. The F. venenatum centromeres were found to be 25% smaller compared to F. graminearum . Chromosome length was 2.8% greater in F. venenatum, primarily due to an increased abundance of repetitive elements and transposons, but not transposon diversity. On chromosome 3 a major sequence rearrangement was found, but its overall gene content was relatively unchanged. Unlike homothallic F. graminearum , heterothallic F. venenatum possessed the MAT1–1 type locus, but lacked the MAT1–2 locus. The F. venenatum genome has the type A trichothecene mycotoxin TRI5 cluster, whereas F. graminearum has type B. From the F. venenatum gene set, 786 predicted proteins were species-specific versus NCBI. The annotated F. venenatum genome was predicted to possess more genes coding for hydrolytic enzymes and species - specific genes involved in the breakdown of polysaccharides than F. graminearum . Comparison of the two genomes reduced the previously defined F. graminearum- specific gene set from 741 to 692 genes. A comparison of the F. graminearum versus F. venenatum proteomes identified 15 putative secondary metabolite gene clusters (SMC), 109 secreted proteins and 38 candidate effectors not found in F. venenatum . Five of the 15 F. graminearum-specific SMCs that were either absent or highly divergent in the F. venenatum genome showed increased in planta expression. In addition, two predicted F. graminearum transcription factors previously shown to be required for fungal virulence on wheat plants were absent or exhibited high sequence divergence. Conclusions This study identifies differences between the F. venenatum and F. graminearum genomes that may contribute to contrasting lifestyles, and highlights the repertoire of F. graminearum -specific candidate genes and SMCs potentially required for pathogenesis.
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Additional file 11: of Inter-genome comparison of the Quorn fungus Fusarium venenatum and the closely related plant infecting pathogen Fusarium graminearum
2018Co-Authors: Robert King, Neil Brown, Martin Urban, Kim Hammond-kosackAbstract:The mating locus within the F. venenatum and F. graminearum genomes. F. venenatum A3/5 contains only the MAT1–1 locus confirming heterothallism and the requirement for a complementary sexual partner. Whilst homothallic F. graminearum possesses both the MAT1–1 and MAT1–2 loci and hence does not require a complementary partner for sexual reproduction. Gene designations: A) FGRRES_08887, B) FGRRES_08888_M, C) FGRRES_08889_M, D) FGRRES_08894, E) FVRRES_05564, F) FGRRES_15525, G) FGRRES_13273_M. (PDF 90 kb
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Additional file 17: of Inter-genome comparison of the Quorn fungus Fusarium venenatum and the closely related plant infecting pathogen Fusarium graminearum
2018Co-Authors: Robert King, Neil Brown, Martin Urban, Kim Hammond-kosackAbstract:Fusarium venenatum presence of TRI6 Fusarium greaminearum binding sites predicted by Nasmith et al. [39]. Fusarium venenatum BLASTP alignment percentages were added to identify presence or absence. (XLS 61Â kb
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Additional file 9: of Inter-genome comparison of the Quorn fungus Fusarium venenatum and the closely related plant infecting pathogen Fusarium graminearum
2018Co-Authors: Robert King, Neil Brown, Martin Urban, Kim Hammond-kosackAbstract:Fusarium graminearum alignment and gene IDâs found in the inverted/translocated regions A/B/C in relation to Fusarium venenatum. Mauve alignments in Sheet 1 of Fusarium venenatum and F. graminearum chromosome 3. The small blue inversion/translocation in Fusarium graminearum has 56 genes, the small green translocation 144 genes, and the large blue/pink inversion/translocation has 586 genes (see Sheet 2â4). Below the top mauve alignment are F. venenatum and F. poae chromosome 3 aligned to F. graminearum using Lastz. (XLS 678Â kb
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Additional file 10: of Inter-genome comparison of the Quorn fungus Fusarium venenatum and the closely related plant infecting pathogen Fusarium graminearum
2018Co-Authors: Robert King, Neil Brown, Martin Urban, Kim Hammond-kosackAbstract:Classes of transposon and repeat found in Fusarium graminearum and Fusarium venenatum. (XLS 37Â kb
Michael Hoff - One of the best experts on this subject based on the ideXlab platform.
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immediate type hypersensitivity reaction to ingestion of mycoprotein quorn in a patient allergic to molds caused by acidic ribosomal protein p2
The Journal of Allergy and Clinical Immunology, 2003Co-Authors: Michael Hoff, Ralph M Trueb, Barbara Ballmerweber, Stefan Vieths, Brunello WuethrichAbstract:BACKGROUND: Quorn is the brand name for a line of foods made with so-called "mycoprotein," which springs from the mold Fusarium venenatum. Since the introduction on the food market, there have been complaints from consumers reporting adverse gastrointestinal reactions after ingestion of mycoprotein. To date, it is not clear whether the reported symptoms are IgE-mediated. OBJECTIVE: The aim of the study was to describe for the first time a case history of an asthmatic patient with severe hypersensitivity reactions to ingested mycoprotein and to identify and characterize the potential allergen that might be responsible for this. METHODS: The sensitization pattern of the asthmatic subject was characterized, and food allergy to mycoprotein was assessed by double-blinded placebo-controlled food challenge. Afterward, specific IgE antibodies of the serum of this patient were used to screen a Fusarium culmorum cDNA expression library. The coding sequence of one enriched cDNA-clone was expressed in Escherichia coli to produce a recombinant protein that was further purified and immunologically characterized. RESULTS: The patient showed high sensitization to many known aeroallergens but apart from Quorn not to any other tested food samples. The deduced amino acid sequence of the enriched cDNA-clone (Fus c 1) showed large identity to the 60S acidic ribosomal protein P2 which is highly conserved among several species and also described as minor allergen in other mold species. The frequency of IgE reactivity of sera from F culmorum -sensitized subjects to rFus c 1 was approximately 35%. By enzyme allergosorbent test inhibition, we found 65% inhibition of mycoprotein IgE reactivity by rFus c 1. On the opposite we found reduced IgE reactivity of rFus c 1 of 68% by using mycoprotein as inhibitor. CONCLUSIONS: Sensitization to mold allergens by the respiratory tract and subsequent oral ingestion of cross-reactive proteins may lead to severe food-allergic reactions. Thus, the 60S acidic ribosomal protein P2 of F venenatum probably is the reason for the described severe hypersensitivity reactions of the patient to Quorn-mycoprotein because of its potential cross-reactivity to the F culmorum allergen Fus c 1.
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Immediate-type hypersensitivity reaction to ingestion of mycoprotein (Quorn) in a patient allergic to molds caused by acidic ribosomal protein P2.
Journal of Allergy and Clinical Immunology, 2003Co-Authors: Michael Hoff, Ralph M Trueb, Stefan Vieths, Barbara Ballmer-weber, Brunello WuethrichAbstract:Abstract Background: Quorn is the brand name for a line of foods made with so-called "mycoprotein," which springs from the mold Fusarium venenatum . Since the introduction on the food market, there have been complaints from consumers reporting adverse gastrointestinal reactions after ingestion of mycoprotein. To date, it is not clear whether the reported symptoms are IgE-mediated. Objective: The aim of the study was to describe for the first time a case history of an asthmatic patient with severe hypersensitivity reactions to ingested mycoprotein and to identify and characterize the potential allergen that might be responsible for this. Methods: The sensitization pattern of the asthmatic subject was characterized, and food allergy to mycoprotein was assessed by double-blinded placebo-controlled food challenge. Afterward, specific IgE antibodies of the serum of this patient were used to screen a Fusarium culmorum cDNA expression library. The coding sequence of one enriched cDNA-clone was expressed in Escherichia coli to produce a recombinant protein that was further purified and immunologically characterized. Results: The patient showed high sensitization to many known aeroallergens but apart from Quorn not to any other tested food samples. The deduced amino acid sequence of the enriched cDNA-clone (Fus c 1) showed large identity to the 60S acidic ribosomal protein P2 which is highly conserved among several species and also described as minor allergen in other mold species. The frequency of IgE reactivity of sera from F culmorum -sensitized subjects to rFus c 1 was approximately 35%. By enzyme allergosorbent test inhibition, we found 65% inhibition of mycoprotein IgE reactivity by rFus c 1 . On the opposite we found reduced IgE reactivity of rFus c 1 of 68% by using mycoprotein as inhibitor. Conclusions: Sensitization to mold allergens by the respiratory tract and subsequent oral ingestion of cross-reactive proteins may lead to severe food-allergic reactions. Thus, the 60S acidic ribosomal protein P2 of F venenatum probably is the reason for the described severe hypersensitivity reactions of the patient to Quorn-mycoprotein because of its potential cross-reactivity to the F culmorum allergen Fus c 1. (J Allergy Clin Immunol 2003;111:1106-10.)