The Experts below are selected from a list of 10452 Experts worldwide ranked by ideXlab platform
Qirong Shen - One of the best experts on this subject based on the ideXlab platform.
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fumigation coupled with bio organic fertilizer for the suppression of watermelon Fusarium Wilt disease re shapes the soil microbiome
Applied Soil Ecology, 2019Co-Authors: Chao Xue, Zongzhuan Shen, Yuewen Hao, Weijie Huang, Yao Chong, Wei Ran, Qirong ShenAbstract:Abstract Fusarium Wilt of watermelon caused by Fusarium oxysporums f. sp. niveum (FON) is the most destructive disease impacting production. Soil fumigation using ammonium bicarbonate coupled with bioorganic fertilizer (BOF) application was conducted in the field in order to suppress Fusarium Wilt disease. The composition of the soil microbiome after fumigation and at harvest was assessed using MiSeq high throughput sequencing. Soil fumigation succeeded in suppressing disease incidence, with a drop from 96% to
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banana Fusarium Wilt disease incidence is influenced by shifts of soil microbial communities under different monoculture spans
Microbial Ecology, 2018Co-Authors: Zongzhuan Shen, Yunze Ruan, Chao Xue, Ryan C Penton, Xianfu Yuan, Qirong ShenAbstract:The continuous cropping of banana in the same field may result in a serious soil-borne Fusarium Wilt disease and a severe yield decline, a phenomenon known as soil sickness. Although soil microorganisms play key roles in maintaining soil health, the alternations of soil microbial community and relationship between these changes and soil sickness under banana monoculture are still unclear. Bacterial and fungal communities in the soil samples collected from banana fields with different monoculture spans were profiled by sequencing of the 16S rRNA genes and internal transcribed spacer using the MiSeq platform to explore the relationship between banana monoculture and Fusarium Wilt disease in the present study. The results showed that successive cropping of banana was significantly correlated with the Fusarium Wilt disease incidence. Fungal communities responded more obviously and quickly to banana consecutive monoculture than bacterial community. Moreover, a higher fungal richness significantly correlated to a higher banana Fusarium Wilt disease incidence but a lower yield. Banana fungal pathogenic genus of Fusarium and Phyllosticta were closely associated with banana yield depletion and disease aggravation. Potential biocontrol agents, such as Funneliformis, Mortierella, Flavobacterium, and Acidobacteria subgroups, exhibited a significant correlation to lower disease occurrence. Further networks analysis revealed that the number of functionally interrelated modules decreased, the composition shifted from bacteria- to fungi-dominated among these modules, and more resources-competitive interactions within networks were observed after banana long-term monoculture. Our results also showed that bacterial and fungal communities were mainly driven by soil organic matter. Overall, the findings indicated that the bacterial and fungal community structures altered significantly after banana long-term monoculture, and the fungal richness, abundance of Fusarium, interactions between and within bacteria and fungi in ecological networks, and soil organic matter were associated with banana soil-borne Fusarium Wilt disease.
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Microflora that harbor the NRPS gene are responsible for Fusarium Wilt disease-suppressive soil
Applied Soil Ecology, 2018Co-Authors: Mengli Zhao, Ruifu Zhang, Xuhui Deng, Jun Yuan, Menghui Dong, Chengzhi Zhu, Qirong ShenAbstract:Abstract Non-ribosomal peptides (NRPs) are one of the largest groups of natural microbial secondary metabolites, which include peptides such as the antibiotics vancomycin and gramicidin, as well as lipopeptides (surfactin, iturin A and bacillomycin). In this study, banana Fusarium Wilt disease suppressive and conducive soils were chosen to investigate the role of microbes that harbor the NRPS gene in disease suppression based on the 454-pyrosequencing platform and real-time PCR technique. The results showed that higher abundances and diversity of microbes that harbor the NRPS gene were observed in the suppressive soil samples than in the conducive soil. According to the results of the DNA sequences blastx of NRPS, the main microbial taxa harboring the NRPS gene were identified, and Pseudomonas in Proteobacteria and Streptomyces in Actinobacteria might be remarkably related to Fusarium Wilt disease suppression. Furthermore, the Mantel test showed that compared with bacteria community and chemical properties, the microbial community harboring the NRPS gene had a more significant impact on the disease incidences of Fusarium Wilt. This study provided non-specific relationships between groups of microbes harboring NRPS genes and Fusarium Wilt disease suppression suggesting potential interaction based on correlation evidence, and pointed out a potential mechanism of suppressive soil.
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Distinct roles for soil fungal and bacterial communities associated with the suppression of vanilla Fusarium Wilt disease
Soil Biology and Biochemistry, 2017Co-Authors: Wu Xiong, Yi Ren, Chen Liu, Qingyun Zhao, Alexandre Jousset, Qirong ShenAbstract:Characterizing microbial communities associated with disease-suppressive soil is an important first step toward understanding the potential of microbiota to protect crops against plant pathogens. In the present study, we compared microbial communities in suppressive- and conducive-soils associated with Fusarium Wilt disease in a vanilla long-term continuous cropping system. Suppressive soil was associated with higher fungal diversity and lower bacterial diversity. The fungal phyla Zygomycota and Basidiomycota, and the bacterial phyla Acidobacteria, Verrucomicrobia, Actinobacteria and Firmicutes were strongly enriched in the suppressive soil. Notably, suppressive soil was dominated by the fungal genus Mortierella, accounting for 37% of the total fungal sequences. The hyper-dominance of Mortierella spp. in suppressive soil suggests that this taxon may serve as an indicator and enhancer of Fusarium Wilt disease suppression in vanilla. In addition, Molecular Ecological Network analysis revealed that fungal communities were more connected and showed more co-occurrence relationships in the suppressive versus conducive soils. Our results indicate that fungal communities may be important in the development of soil suppressiveness against vanilla Fusarium Wilt disease.
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Nitrate Increased Cucumber Tolerance to Fusarium Wilt by Regulating Fungal Toxin Production and Distribution
MDPI AG, 2017Co-Authors: Jinyan Zhou, Qirong Shen, Min Wang, Yuming Sun, Ruirui Wang, Asanjan Saydin, Shiwei GuoAbstract:Cucumber Fusarium Wilt, induced by Fusarium oxysporum f. sp. cucumerinum (FOC), causes severe losses in cucumber yield and quality. Nitrogen (N), as the most important mineral nutrient for plants, plays a critical role in plant–pathogen interactions. Hydroponic assays were conducted to investigate the effects of different N forms (NH4+ vs. NO3‒) and supply levels (low, 1 mM; high, 5 mM) on cucumber Fusarium Wilt. The NO3‒-fed cucumber plants were more tolerant to Fusarium Wilt compared with NH4+-fed plants, and accompanied by lower leaf temperature after FOC infection. The disease index decreased as the NO3‒ supply increased but increased with the NH4+ level supplied. Although the FOC grew better under high NO3− in vitro, FOC colonization and fusaric acid (FA) production decreased in cucumber plants under high NO3− supply, associated with lower leaf membrane injury. There was a positive correlation between the FA content and the FOC number or relative membrane injury. After the exogenous application of FA, less FA accumulated in the leaves under NO3− feeding, accompanied with a lower leaf membrane injury. In conclusion, higher NO3− supply protected cucumber plants against Fusarium Wilt by suppressing FOC colonization and FA production in plants, and increasing the plant tolerance to FA
Zongzhuan Shen - One of the best experts on this subject based on the ideXlab platform.
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fumigation coupled with bio organic fertilizer for the suppression of watermelon Fusarium Wilt disease re shapes the soil microbiome
Applied Soil Ecology, 2019Co-Authors: Chao Xue, Zongzhuan Shen, Yuewen Hao, Weijie Huang, Yao Chong, Wei Ran, Qirong ShenAbstract:Abstract Fusarium Wilt of watermelon caused by Fusarium oxysporums f. sp. niveum (FON) is the most destructive disease impacting production. Soil fumigation using ammonium bicarbonate coupled with bioorganic fertilizer (BOF) application was conducted in the field in order to suppress Fusarium Wilt disease. The composition of the soil microbiome after fumigation and at harvest was assessed using MiSeq high throughput sequencing. Soil fumigation succeeded in suppressing disease incidence, with a drop from 96% to
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banana Fusarium Wilt disease incidence is influenced by shifts of soil microbial communities under different monoculture spans
Microbial Ecology, 2018Co-Authors: Zongzhuan Shen, Yunze Ruan, Chao Xue, Ryan C Penton, Xianfu Yuan, Qirong ShenAbstract:The continuous cropping of banana in the same field may result in a serious soil-borne Fusarium Wilt disease and a severe yield decline, a phenomenon known as soil sickness. Although soil microorganisms play key roles in maintaining soil health, the alternations of soil microbial community and relationship between these changes and soil sickness under banana monoculture are still unclear. Bacterial and fungal communities in the soil samples collected from banana fields with different monoculture spans were profiled by sequencing of the 16S rRNA genes and internal transcribed spacer using the MiSeq platform to explore the relationship between banana monoculture and Fusarium Wilt disease in the present study. The results showed that successive cropping of banana was significantly correlated with the Fusarium Wilt disease incidence. Fungal communities responded more obviously and quickly to banana consecutive monoculture than bacterial community. Moreover, a higher fungal richness significantly correlated to a higher banana Fusarium Wilt disease incidence but a lower yield. Banana fungal pathogenic genus of Fusarium and Phyllosticta were closely associated with banana yield depletion and disease aggravation. Potential biocontrol agents, such as Funneliformis, Mortierella, Flavobacterium, and Acidobacteria subgroups, exhibited a significant correlation to lower disease occurrence. Further networks analysis revealed that the number of functionally interrelated modules decreased, the composition shifted from bacteria- to fungi-dominated among these modules, and more resources-competitive interactions within networks were observed after banana long-term monoculture. Our results also showed that bacterial and fungal communities were mainly driven by soil organic matter. Overall, the findings indicated that the bacterial and fungal community structures altered significantly after banana long-term monoculture, and the fungal richness, abundance of Fusarium, interactions between and within bacteria and fungi in ecological networks, and soil organic matter were associated with banana soil-borne Fusarium Wilt disease.
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inducing the rhizosphere microbiome by biofertilizer application to suppress banana Fusarium Wilt disease
Soil Biology & Biochemistry, 2017Co-Authors: Ryan C Penton, Yunze Ruan, Zongzhuan Shen, Chao Xue, Qirong ShenAbstract:Worldwide, banana production is severely hindered by Fusarium Wilt, a devastating disease caused by the soil-borne fungus Fusarium oxysporum f. sp. cubense (Foc). With no widely adopted efficient method of control or prevention, the emergence of a new Foc variant, tropical race 4 (Foc TR4), has led to the widespread destruction of plantations in Cavendish-producing areas. Recently, banana Fusarium Wilt has been controlled by the consecutive application of biofertilizer (BIO) in newly reclaimed fields. In this study we examine the temporal effects of BIO versus compost application in newly converted banana fields on the composition and abundance of the rhizosphere bacterial and fungal communities and the survival of the biocontrol inoculant, Bacillus amyloliquefaciens NJN-6. Our findings show that BIO-amended rhizosphere soils increased the abundance of bacteria while decreasing fungal abundance. This corresponded to higher bacterial richness and diversity in the BIO amendment, while no trends were observed with the fungal community. Rhizosphere soil bacterial and fungal community composition were significantly different between BIO and compost amendment and treatment, not time, exhibited the largest impact. Other potential taxa involved in disease suppression were also identified, such as increased abundances of Sphingobium, Dyadobacter, and Cryptococcus and lower abundances of Fusarium, Ralstonia, and Burkholderia. Overall, decreased abundances of F. oxysporum and a lack of variability in the abundance of the biocontrol agent NJN-6 over three years contributed to disease suppression, in combination with alterations in fungal and bacterial composition and abundance, pointing to the sustainability of BIO as an amendment for disease suppression.
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Novel soil fumigation method for suppressing cucumber Fusarium Wilt disease associated with soil microflora alterations
Applied Soil Ecology, 2016Co-Authors: Zongzhuan Shen, Ruifu Zhang, Li Sun, Xuhui Deng, Qirong ShenAbstract:Fusarium Wilt in cucumber results from continuous cropping and is a serious soil-borne fungal disease that threatens cucumber production around the world. The application of a novel fumigation agent based on ammonium bicarbonate to the soil as a strategy for controlling Fusarium Wilt and its effects on soil microflora was investigated in a field with serious disease incidence in this study. Overall, the results showed that fumigation effectively controlled cucumber Fusarium Wilt disease and significantly increased the total and mean cucumber fruit weight. Real-Time PCR results showed that the total bacterial and fungal numbers in the treatment (LAB) significantly decreased after fumigation and that significantly fewer fungi were observed after harvest (LABOF). The next-generation sequencing of the 16S rRNA and internal transcribed spacer (ITS) genes using MiSeq platform showed that the soil bacterial and fungal community structures in the fumigation treatment were significantly different from the control without fumigation regardless after fumigation or harvest. Compared to the control, higher abundances of Gemmatimonadetes, Verrucomicrobia and Zygomycota, and lower abundance of Ascomycota were observed in the fumigated soils after fumigation and harvest. Furthermore, the abundances of Mortierella and Gp1 were significantly higher. Most importantly, the abundance of Fusarium, which includes the pathogen potentially responsible for cucumber Fusarium Wilt disease, was significantly lower in the fumigated soils after harvest. Redundancy analysis showed that the fumigated soils were dominated by Ohtaekwangia, Gp6, and Gp4, which were related to the soil total nitrogen (TN), ammonium nitrogen (NH4–N) and nitrate nitrogen (NO3–N) contents. In addition, Penicillium and Pseudaleuria fungi were dominant in the treatment and control, and the control was dominated by Fusarium. In conclusion, the observed disease suppression due to the novel ecological soil fumigation strategy may be attributed to general suppression resulting from altered soil properties, such as higher soil NH4–N, NO3–N and TON contents, and the alteration of the disturbed soil microflora in a cucumber monoculture system.
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soils naturally suppressive to banana Fusarium Wilt disease harbor unique bacterial communities
Plant and Soil, 2015Co-Authors: Zongzhuan Shen, Yunze Ruan, Chao Xue, Shutang Zhong, Qirong ShenAbstract:Banana Fusarium Wilt disease is caused by the Fusarium oxysporum f. sp. cubense race 4 fungus and is a vast problem for global banana production. Suppressive and conducive soils were analyzed to characterize important microbial populations and soil chemical properties that contribute to disease suppressiveness. Soil bacteria communities from the two banana orchards with excellent Fusarium disease suppression (suppressive soil) after long-term monoculture and two adjacent banana orchards with serious Fusarium Wilt disease (conducive soils) were compared using deep 16S RNA barcode pyrosequencing. Compared to the conducive soils within the same field site, higher (P < 0.05) richness and diversity indices were observed in both suppressive soils. Moreover, more operational taxonomic units (OTUs) were observed in the two suppressive soils. Hierarchical cluster analyses showed that bacterial community membership and structure in disease-suppressive soils differed from disease-conducive soils. The Acidobacteria phylum was significantly (P < 0.05) elevated, but Bacteroidetes was significantly (P < 0.05) reduced in suppressive soils. The Gp4, Gp5, Chthonomonas, Pseudomonas, and Tumebacillus genera were significantly (P < 0.05) enriched in suppressive soils, but Gp2 was significantly (P < 0.05) reduced in suppressive soils. Furthermore, the enrichment of Gp5 and Pseudomonas as well as the soil physicochemical properties of available phosphorus were significantly (P < 0.05) correlated with disease suppression. Naturally disease suppressive soils to banana Fusarium Wilt disease harbor unique bacterial communities.
Chao Xue - One of the best experts on this subject based on the ideXlab platform.
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Manipulating the soil microbiomes during a community recovery process with plant beneficial species for the suppression of Fusarium Wilt of watermelon
'Springer Science and Business Media LLC', 2021Co-Authors: Xu Zhang, Chao Xue, Dan Fang, Mengyu Wei, Chenjin Zhuo, Junyao Jin, Biao Shen, Ning LingAbstract:Abstract Fusarium Wilt is a devastating disease which impacts watermelon production. Soil fumigation using dazomet followed by biological organic fertilizer was applied to suppress the Fusarium Wilt disease. We propose that fumigation suppresses the soil indigenous community, especially the soil-borne pathogens, while the utilization of bio-organic fertilizer facilitates the recovery of the soil microbiome to a beneficial, suppressive state through the introduction of plant growth-promoting microorganisms. Greenhouse experiment showed that applied biological organic fertilizer after dazomet fumigation effective restrain the disease incidence with a 93.6% disease control. Fumigation strongly decreased soil microbial diversity and altered relative taxa abundances, suggesting the possibility of niche release by the resident soil microbial community. Fumigation followed by bio-fertilizer transformed the soil microbial community composition and resulted in higher relative abundances of beneficial microbial groups such as Bacillus (8.5%) and Trichoderma (13.5%), coupled with lower Fusarium abundance compared to other treatments. Network analysis illustrated that soil fumigation decreased interactions within the soil microbial community with less nodes and links while bio-fertilizer addition promoted node interactions. In addition, bio-fertilizer addition after fumigation resulted in the beneficial species becoming the key network connectors. Collectively, fumigation appears to release the resident soil niche resulting in lower diversity while the beneficial microbes introduced by bio-fertilizer addition colonize these niches, leading to a more complex community with fewer pathogens that suppresses Fusarium Wilt disease incidence
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fumigation coupled with bio organic fertilizer for the suppression of watermelon Fusarium Wilt disease re shapes the soil microbiome
Applied Soil Ecology, 2019Co-Authors: Chao Xue, Zongzhuan Shen, Yuewen Hao, Weijie Huang, Yao Chong, Wei Ran, Qirong ShenAbstract:Abstract Fusarium Wilt of watermelon caused by Fusarium oxysporums f. sp. niveum (FON) is the most destructive disease impacting production. Soil fumigation using ammonium bicarbonate coupled with bioorganic fertilizer (BOF) application was conducted in the field in order to suppress Fusarium Wilt disease. The composition of the soil microbiome after fumigation and at harvest was assessed using MiSeq high throughput sequencing. Soil fumigation succeeded in suppressing disease incidence, with a drop from 96% to
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banana Fusarium Wilt disease incidence is influenced by shifts of soil microbial communities under different monoculture spans
Microbial Ecology, 2018Co-Authors: Zongzhuan Shen, Yunze Ruan, Chao Xue, Ryan C Penton, Xianfu Yuan, Qirong ShenAbstract:The continuous cropping of banana in the same field may result in a serious soil-borne Fusarium Wilt disease and a severe yield decline, a phenomenon known as soil sickness. Although soil microorganisms play key roles in maintaining soil health, the alternations of soil microbial community and relationship between these changes and soil sickness under banana monoculture are still unclear. Bacterial and fungal communities in the soil samples collected from banana fields with different monoculture spans were profiled by sequencing of the 16S rRNA genes and internal transcribed spacer using the MiSeq platform to explore the relationship between banana monoculture and Fusarium Wilt disease in the present study. The results showed that successive cropping of banana was significantly correlated with the Fusarium Wilt disease incidence. Fungal communities responded more obviously and quickly to banana consecutive monoculture than bacterial community. Moreover, a higher fungal richness significantly correlated to a higher banana Fusarium Wilt disease incidence but a lower yield. Banana fungal pathogenic genus of Fusarium and Phyllosticta were closely associated with banana yield depletion and disease aggravation. Potential biocontrol agents, such as Funneliformis, Mortierella, Flavobacterium, and Acidobacteria subgroups, exhibited a significant correlation to lower disease occurrence. Further networks analysis revealed that the number of functionally interrelated modules decreased, the composition shifted from bacteria- to fungi-dominated among these modules, and more resources-competitive interactions within networks were observed after banana long-term monoculture. Our results also showed that bacterial and fungal communities were mainly driven by soil organic matter. Overall, the findings indicated that the bacterial and fungal community structures altered significantly after banana long-term monoculture, and the fungal richness, abundance of Fusarium, interactions between and within bacteria and fungi in ecological networks, and soil organic matter were associated with banana soil-borne Fusarium Wilt disease.
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inducing the rhizosphere microbiome by biofertilizer application to suppress banana Fusarium Wilt disease
Soil Biology & Biochemistry, 2017Co-Authors: Ryan C Penton, Yunze Ruan, Zongzhuan Shen, Chao Xue, Qirong ShenAbstract:Worldwide, banana production is severely hindered by Fusarium Wilt, a devastating disease caused by the soil-borne fungus Fusarium oxysporum f. sp. cubense (Foc). With no widely adopted efficient method of control or prevention, the emergence of a new Foc variant, tropical race 4 (Foc TR4), has led to the widespread destruction of plantations in Cavendish-producing areas. Recently, banana Fusarium Wilt has been controlled by the consecutive application of biofertilizer (BIO) in newly reclaimed fields. In this study we examine the temporal effects of BIO versus compost application in newly converted banana fields on the composition and abundance of the rhizosphere bacterial and fungal communities and the survival of the biocontrol inoculant, Bacillus amyloliquefaciens NJN-6. Our findings show that BIO-amended rhizosphere soils increased the abundance of bacteria while decreasing fungal abundance. This corresponded to higher bacterial richness and diversity in the BIO amendment, while no trends were observed with the fungal community. Rhizosphere soil bacterial and fungal community composition were significantly different between BIO and compost amendment and treatment, not time, exhibited the largest impact. Other potential taxa involved in disease suppression were also identified, such as increased abundances of Sphingobium, Dyadobacter, and Cryptococcus and lower abundances of Fusarium, Ralstonia, and Burkholderia. Overall, decreased abundances of F. oxysporum and a lack of variability in the abundance of the biocontrol agent NJN-6 over three years contributed to disease suppression, in combination with alterations in fungal and bacterial composition and abundance, pointing to the sustainability of BIO as an amendment for disease suppression.
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soils naturally suppressive to banana Fusarium Wilt disease harbor unique bacterial communities
Plant and Soil, 2015Co-Authors: Zongzhuan Shen, Yunze Ruan, Chao Xue, Shutang Zhong, Qirong ShenAbstract:Banana Fusarium Wilt disease is caused by the Fusarium oxysporum f. sp. cubense race 4 fungus and is a vast problem for global banana production. Suppressive and conducive soils were analyzed to characterize important microbial populations and soil chemical properties that contribute to disease suppressiveness. Soil bacteria communities from the two banana orchards with excellent Fusarium disease suppression (suppressive soil) after long-term monoculture and two adjacent banana orchards with serious Fusarium Wilt disease (conducive soils) were compared using deep 16S RNA barcode pyrosequencing. Compared to the conducive soils within the same field site, higher (P < 0.05) richness and diversity indices were observed in both suppressive soils. Moreover, more operational taxonomic units (OTUs) were observed in the two suppressive soils. Hierarchical cluster analyses showed that bacterial community membership and structure in disease-suppressive soils differed from disease-conducive soils. The Acidobacteria phylum was significantly (P < 0.05) elevated, but Bacteroidetes was significantly (P < 0.05) reduced in suppressive soils. The Gp4, Gp5, Chthonomonas, Pseudomonas, and Tumebacillus genera were significantly (P < 0.05) enriched in suppressive soils, but Gp2 was significantly (P < 0.05) reduced in suppressive soils. Furthermore, the enrichment of Gp5 and Pseudomonas as well as the soil physicochemical properties of available phosphorus were significantly (P < 0.05) correlated with disease suppression. Naturally disease suppressive soils to banana Fusarium Wilt disease harbor unique bacterial communities.
Yunze Ruan - One of the best experts on this subject based on the ideXlab platform.
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banana Fusarium Wilt disease incidence is influenced by shifts of soil microbial communities under different monoculture spans
Microbial Ecology, 2018Co-Authors: Zongzhuan Shen, Yunze Ruan, Chao Xue, Ryan C Penton, Xianfu Yuan, Qirong ShenAbstract:The continuous cropping of banana in the same field may result in a serious soil-borne Fusarium Wilt disease and a severe yield decline, a phenomenon known as soil sickness. Although soil microorganisms play key roles in maintaining soil health, the alternations of soil microbial community and relationship between these changes and soil sickness under banana monoculture are still unclear. Bacterial and fungal communities in the soil samples collected from banana fields with different monoculture spans were profiled by sequencing of the 16S rRNA genes and internal transcribed spacer using the MiSeq platform to explore the relationship between banana monoculture and Fusarium Wilt disease in the present study. The results showed that successive cropping of banana was significantly correlated with the Fusarium Wilt disease incidence. Fungal communities responded more obviously and quickly to banana consecutive monoculture than bacterial community. Moreover, a higher fungal richness significantly correlated to a higher banana Fusarium Wilt disease incidence but a lower yield. Banana fungal pathogenic genus of Fusarium and Phyllosticta were closely associated with banana yield depletion and disease aggravation. Potential biocontrol agents, such as Funneliformis, Mortierella, Flavobacterium, and Acidobacteria subgroups, exhibited a significant correlation to lower disease occurrence. Further networks analysis revealed that the number of functionally interrelated modules decreased, the composition shifted from bacteria- to fungi-dominated among these modules, and more resources-competitive interactions within networks were observed after banana long-term monoculture. Our results also showed that bacterial and fungal communities were mainly driven by soil organic matter. Overall, the findings indicated that the bacterial and fungal community structures altered significantly after banana long-term monoculture, and the fungal richness, abundance of Fusarium, interactions between and within bacteria and fungi in ecological networks, and soil organic matter were associated with banana soil-borne Fusarium Wilt disease.
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inducing the rhizosphere microbiome by biofertilizer application to suppress banana Fusarium Wilt disease
Soil Biology & Biochemistry, 2017Co-Authors: Ryan C Penton, Yunze Ruan, Zongzhuan Shen, Chao Xue, Qirong ShenAbstract:Worldwide, banana production is severely hindered by Fusarium Wilt, a devastating disease caused by the soil-borne fungus Fusarium oxysporum f. sp. cubense (Foc). With no widely adopted efficient method of control or prevention, the emergence of a new Foc variant, tropical race 4 (Foc TR4), has led to the widespread destruction of plantations in Cavendish-producing areas. Recently, banana Fusarium Wilt has been controlled by the consecutive application of biofertilizer (BIO) in newly reclaimed fields. In this study we examine the temporal effects of BIO versus compost application in newly converted banana fields on the composition and abundance of the rhizosphere bacterial and fungal communities and the survival of the biocontrol inoculant, Bacillus amyloliquefaciens NJN-6. Our findings show that BIO-amended rhizosphere soils increased the abundance of bacteria while decreasing fungal abundance. This corresponded to higher bacterial richness and diversity in the BIO amendment, while no trends were observed with the fungal community. Rhizosphere soil bacterial and fungal community composition were significantly different between BIO and compost amendment and treatment, not time, exhibited the largest impact. Other potential taxa involved in disease suppression were also identified, such as increased abundances of Sphingobium, Dyadobacter, and Cryptococcus and lower abundances of Fusarium, Ralstonia, and Burkholderia. Overall, decreased abundances of F. oxysporum and a lack of variability in the abundance of the biocontrol agent NJN-6 over three years contributed to disease suppression, in combination with alterations in fungal and bacterial composition and abundance, pointing to the sustainability of BIO as an amendment for disease suppression.
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continous application of bioorganic fertilizer induced resilient culturable bacteria community associated with banana Fusarium Wilt suppression
Scientific Reports, 2016Co-Authors: Lin Fu, Yunze Ruan, Rong Li, Qirong ShenAbstract:Fusarium Wilt of banana always drives farmers to find new land for banana cultivation due to the comeback of the disease after a few cropping years. A novel idea for solving this problem is the continuous application of bioorganic fertilizer (BIO), which should be practiced from the beginning of banana planting. In this study, BIO was applied in newly reclaimed fields to pre-control banana Fusarium Wilt and the culturable rhizobacteria community were evaluated using Biolog Ecoplates and culture-dependent denaturing gradient gel electrophoresis (CD-DGGE). The results showed that BIO application significantly reduced disease incidences and increased crop yields, respectivly. And the stabilized general bacterial metabolic potential, especially for the utilization of carbohydrates, carboxylic acids and phenolic compounds, was induced by BIO application. DGGE profiles demonstrated that resilient community structure of culturable rhizobacteria with higher richness and diversity were observed in BIO treated soils. Morever, enriched culturable bacteria affiliated with Firmicutes, Gammaproteobacteria and Actinobacteria were also detected. In total, continuous application of BIO effectively suppressed Fusarium Wilt disease by stabilizing culturable bacterial metabolic potential and community structure. This study revealed a new method to control Fusarium Wilt of banana for long term banana cultivation.
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soils naturally suppressive to banana Fusarium Wilt disease harbor unique bacterial communities
Plant and Soil, 2015Co-Authors: Zongzhuan Shen, Yunze Ruan, Chao Xue, Shutang Zhong, Qirong ShenAbstract:Banana Fusarium Wilt disease is caused by the Fusarium oxysporum f. sp. cubense race 4 fungus and is a vast problem for global banana production. Suppressive and conducive soils were analyzed to characterize important microbial populations and soil chemical properties that contribute to disease suppressiveness. Soil bacteria communities from the two banana orchards with excellent Fusarium disease suppression (suppressive soil) after long-term monoculture and two adjacent banana orchards with serious Fusarium Wilt disease (conducive soils) were compared using deep 16S RNA barcode pyrosequencing. Compared to the conducive soils within the same field site, higher (P < 0.05) richness and diversity indices were observed in both suppressive soils. Moreover, more operational taxonomic units (OTUs) were observed in the two suppressive soils. Hierarchical cluster analyses showed that bacterial community membership and structure in disease-suppressive soils differed from disease-conducive soils. The Acidobacteria phylum was significantly (P < 0.05) elevated, but Bacteroidetes was significantly (P < 0.05) reduced in suppressive soils. The Gp4, Gp5, Chthonomonas, Pseudomonas, and Tumebacillus genera were significantly (P < 0.05) enriched in suppressive soils, but Gp2 was significantly (P < 0.05) reduced in suppressive soils. Furthermore, the enrichment of Gp5 and Pseudomonas as well as the soil physicochemical properties of available phosphorus were significantly (P < 0.05) correlated with disease suppression. Naturally disease suppressive soils to banana Fusarium Wilt disease harbor unique bacterial communities.
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soils naturally suppressive to banana Fusarium Wilt disease harbor unique bacterial communities
Plant and Soil, 2015Co-Authors: Zongzhuan Shen, Yunze Ruan, Chao Xue, Shutang Zhong, Qirong ShenAbstract:Aims Banana Fusarium Wilt disease is caused by the Fusarium oxysporum f. sp. cubense race 4 fungus and is a vast problem for global banana production. Suppressive and conducive soils were analyzed to characterize important microbial populations and soil chemical properties that contribute to disease suppressiveness.
Ruifu Zhang - One of the best experts on this subject based on the ideXlab platform.
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Microflora that harbor the NRPS gene are responsible for Fusarium Wilt disease-suppressive soil
Applied Soil Ecology, 2018Co-Authors: Mengli Zhao, Ruifu Zhang, Xuhui Deng, Jun Yuan, Menghui Dong, Chengzhi Zhu, Qirong ShenAbstract:Abstract Non-ribosomal peptides (NRPs) are one of the largest groups of natural microbial secondary metabolites, which include peptides such as the antibiotics vancomycin and gramicidin, as well as lipopeptides (surfactin, iturin A and bacillomycin). In this study, banana Fusarium Wilt disease suppressive and conducive soils were chosen to investigate the role of microbes that harbor the NRPS gene in disease suppression based on the 454-pyrosequencing platform and real-time PCR technique. The results showed that higher abundances and diversity of microbes that harbor the NRPS gene were observed in the suppressive soil samples than in the conducive soil. According to the results of the DNA sequences blastx of NRPS, the main microbial taxa harboring the NRPS gene were identified, and Pseudomonas in Proteobacteria and Streptomyces in Actinobacteria might be remarkably related to Fusarium Wilt disease suppression. Furthermore, the Mantel test showed that compared with bacteria community and chemical properties, the microbial community harboring the NRPS gene had a more significant impact on the disease incidences of Fusarium Wilt. This study provided non-specific relationships between groups of microbes harboring NRPS genes and Fusarium Wilt disease suppression suggesting potential interaction based on correlation evidence, and pointed out a potential mechanism of suppressive soil.
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Novel soil fumigation method for suppressing cucumber Fusarium Wilt disease associated with soil microflora alterations
Applied Soil Ecology, 2016Co-Authors: Zongzhuan Shen, Ruifu Zhang, Li Sun, Xuhui Deng, Qirong ShenAbstract:Fusarium Wilt in cucumber results from continuous cropping and is a serious soil-borne fungal disease that threatens cucumber production around the world. The application of a novel fumigation agent based on ammonium bicarbonate to the soil as a strategy for controlling Fusarium Wilt and its effects on soil microflora was investigated in a field with serious disease incidence in this study. Overall, the results showed that fumigation effectively controlled cucumber Fusarium Wilt disease and significantly increased the total and mean cucumber fruit weight. Real-Time PCR results showed that the total bacterial and fungal numbers in the treatment (LAB) significantly decreased after fumigation and that significantly fewer fungi were observed after harvest (LABOF). The next-generation sequencing of the 16S rRNA and internal transcribed spacer (ITS) genes using MiSeq platform showed that the soil bacterial and fungal community structures in the fumigation treatment were significantly different from the control without fumigation regardless after fumigation or harvest. Compared to the control, higher abundances of Gemmatimonadetes, Verrucomicrobia and Zygomycota, and lower abundance of Ascomycota were observed in the fumigated soils after fumigation and harvest. Furthermore, the abundances of Mortierella and Gp1 were significantly higher. Most importantly, the abundance of Fusarium, which includes the pathogen potentially responsible for cucumber Fusarium Wilt disease, was significantly lower in the fumigated soils after harvest. Redundancy analysis showed that the fumigated soils were dominated by Ohtaekwangia, Gp6, and Gp4, which were related to the soil total nitrogen (TN), ammonium nitrogen (NH4–N) and nitrate nitrogen (NO3–N) contents. In addition, Penicillium and Pseudaleuria fungi were dominant in the treatment and control, and the control was dominated by Fusarium. In conclusion, the observed disease suppression due to the novel ecological soil fumigation strategy may be attributed to general suppression resulting from altered soil properties, such as higher soil NH4–N, NO3–N and TON contents, and the alteration of the disturbed soil microflora in a cucumber monoculture system.
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application of bio organic fertilizer can control Fusarium Wilt of cucumber plants by regulating microbial community of rhizosphere soil
Biology and Fertility of Soils, 2012Co-Authors: Meihua Qiu, Ruifu Zhang, Chao Xue, Shusheng Zhang, Nan Zhang, Qirong ShenAbstract:Fusarium Wilt, caused by Fusarium oxysporum f. sp. cucumerinum J. H. Owen, results in considerable yield losses for cucumber plants. A bio-organic fertilizer (BIO), which was a combination of manure composts with antagonistic microorganisms, and an organic fertilizer (OF) were evaluated for their efficiencies in controlling Fusarium Wilt. Application of the BIO suppressed the disease incidence by 83% and reduced yield losses threefold compared with the application of OF. Analysis of microbial communities in rhizosphere soils by high-throughput pyrosequencing showed that more complex community structures were present in BIO than in OF treated soils. The dominant taxonomic phyla found in both samples were Proteobacteria, Firmicutes, Actinobacteria and Acidobacteria among bacteria and Ascomycota among fungi. Abundance of beneficial bacteria or fungi, such as Trichoderma, Hypoxylon, Tritirachium, Paenibacillus, Bacillus, Haliangium and Streptomyces, increased compared to the OF treatment, whereas the soil-borne pathogen, Fusarium, was markedly decreased. Overall, the results of this study demonstrate that the application of the BIO was a useful and effective approach to suppress Fusarium Wilt and that the high-throughput 454 pyrosequencing was a suitable method for the characterization of microbial communities of rhizosphere soil of cucumber.
