The Experts below are selected from a list of 2517 Experts worldwide ranked by ideXlab platform
Silvana Zanlungo - One of the best experts on this subject based on the ideXlab platform.
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Gadolinium Chloride Rescues Niemann–Pick Type C Liver Damage
International journal of molecular sciences, 2018Co-Authors: Andrés D. Klein, Juan Esteban Oyarzún, Cristian Cortez, Silvana ZanlungoAbstract:Niemann–Pick type C (NPC) disease is a rare neurovisceral cholesterol storage disorder that arises from loss of function mutations in the NPC1 or NPC2 genes. Soon after birth, some patients present with an aggressive hepatosplenomegaly and cholestatic signs. Histopathologically, the liver presents with large numbers of foam cells; however, their role in disease pathogenesis has not been explored in depth. Here, we studied the consequences of Gadolinium Chloride (GdCl3) treatment, a well-known Kupffer/foam cell inhibitor, at late stages of NPC liver disease and compared it with NPC1 genetic rescue in hepatocytes in vivo. GdCl3 treatment successfully blocked the endocytic capacity of hepatic Kupffer/foam measured by India ink endocytosis, decreased the levels CD68—A marker of Kupffer cells in the liver—and normalized the transaminase levels in serum of NPC mice to a similar extent to those obtained by genetic Npc1 rescue of liver cells. Gadolinium salts are widely used as magnetic resonance imaging (MRI) contrasts. This study opens the possibility of targeting foam cells with Gadolinium or by other means for improving NPC liver disease. Synopsis: Gadolinium Chloride can effectively rescue some parameters of liver dysfunction in NPC mice and its potential use in patients should be carefully evaluated.
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Gadolinium Chloride rescues niemann pick type c liver damage
International Journal of Molecular Sciences, 2018Co-Authors: Andrés D. Klein, Juan Esteban Oyarzún, Cristian Cortez, Silvana ZanlungoAbstract:Niemann–Pick type C (NPC) disease is a rare neurovisceral cholesterol storage disorder that arises from loss of function mutations in the NPC1 or NPC2 genes. Soon after birth, some patients present with an aggressive hepatosplenomegaly and cholestatic signs. Histopathologically, the liver presents with large numbers of foam cells; however, their role in disease pathogenesis has not been explored in depth. Here, we studied the consequences of Gadolinium Chloride (GdCl3) treatment, a well-known Kupffer/foam cell inhibitor, at late stages of NPC liver disease and compared it with NPC1 genetic rescue in hepatocytes in vivo. GdCl3 treatment successfully blocked the endocytic capacity of hepatic Kupffer/foam measured by India ink endocytosis, decreased the levels CD68—A marker of Kupffer cells in the liver—and normalized the transaminase levels in serum of NPC mice to a similar extent to those obtained by genetic Npc1 rescue of liver cells. Gadolinium salts are widely used as magnetic resonance imaging (MRI) contrasts. This study opens the possibility of targeting foam cells with Gadolinium or by other means for improving NPC liver disease. Synopsis: Gadolinium Chloride can effectively rescue some parameters of liver dysfunction in NPC mice and its potential use in patients should be carefully evaluated.
Andrés D. Klein - One of the best experts on this subject based on the ideXlab platform.
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Gadolinium Chloride Rescues Niemann–Pick Type C Liver Damage
International journal of molecular sciences, 2018Co-Authors: Andrés D. Klein, Juan Esteban Oyarzún, Cristian Cortez, Silvana ZanlungoAbstract:Niemann–Pick type C (NPC) disease is a rare neurovisceral cholesterol storage disorder that arises from loss of function mutations in the NPC1 or NPC2 genes. Soon after birth, some patients present with an aggressive hepatosplenomegaly and cholestatic signs. Histopathologically, the liver presents with large numbers of foam cells; however, their role in disease pathogenesis has not been explored in depth. Here, we studied the consequences of Gadolinium Chloride (GdCl3) treatment, a well-known Kupffer/foam cell inhibitor, at late stages of NPC liver disease and compared it with NPC1 genetic rescue in hepatocytes in vivo. GdCl3 treatment successfully blocked the endocytic capacity of hepatic Kupffer/foam measured by India ink endocytosis, decreased the levels CD68—A marker of Kupffer cells in the liver—and normalized the transaminase levels in serum of NPC mice to a similar extent to those obtained by genetic Npc1 rescue of liver cells. Gadolinium salts are widely used as magnetic resonance imaging (MRI) contrasts. This study opens the possibility of targeting foam cells with Gadolinium or by other means for improving NPC liver disease. Synopsis: Gadolinium Chloride can effectively rescue some parameters of liver dysfunction in NPC mice and its potential use in patients should be carefully evaluated.
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Gadolinium Chloride rescues niemann pick type c liver damage
International Journal of Molecular Sciences, 2018Co-Authors: Andrés D. Klein, Juan Esteban Oyarzún, Cristian Cortez, Silvana ZanlungoAbstract:Niemann–Pick type C (NPC) disease is a rare neurovisceral cholesterol storage disorder that arises from loss of function mutations in the NPC1 or NPC2 genes. Soon after birth, some patients present with an aggressive hepatosplenomegaly and cholestatic signs. Histopathologically, the liver presents with large numbers of foam cells; however, their role in disease pathogenesis has not been explored in depth. Here, we studied the consequences of Gadolinium Chloride (GdCl3) treatment, a well-known Kupffer/foam cell inhibitor, at late stages of NPC liver disease and compared it with NPC1 genetic rescue in hepatocytes in vivo. GdCl3 treatment successfully blocked the endocytic capacity of hepatic Kupffer/foam measured by India ink endocytosis, decreased the levels CD68—A marker of Kupffer cells in the liver—and normalized the transaminase levels in serum of NPC mice to a similar extent to those obtained by genetic Npc1 rescue of liver cells. Gadolinium salts are widely used as magnetic resonance imaging (MRI) contrasts. This study opens the possibility of targeting foam cells with Gadolinium or by other means for improving NPC liver disease. Synopsis: Gadolinium Chloride can effectively rescue some parameters of liver dysfunction in NPC mice and its potential use in patients should be carefully evaluated.
Cristian Cortez - One of the best experts on this subject based on the ideXlab platform.
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Gadolinium Chloride Rescues Niemann–Pick Type C Liver Damage
International journal of molecular sciences, 2018Co-Authors: Andrés D. Klein, Juan Esteban Oyarzún, Cristian Cortez, Silvana ZanlungoAbstract:Niemann–Pick type C (NPC) disease is a rare neurovisceral cholesterol storage disorder that arises from loss of function mutations in the NPC1 or NPC2 genes. Soon after birth, some patients present with an aggressive hepatosplenomegaly and cholestatic signs. Histopathologically, the liver presents with large numbers of foam cells; however, their role in disease pathogenesis has not been explored in depth. Here, we studied the consequences of Gadolinium Chloride (GdCl3) treatment, a well-known Kupffer/foam cell inhibitor, at late stages of NPC liver disease and compared it with NPC1 genetic rescue in hepatocytes in vivo. GdCl3 treatment successfully blocked the endocytic capacity of hepatic Kupffer/foam measured by India ink endocytosis, decreased the levels CD68—A marker of Kupffer cells in the liver—and normalized the transaminase levels in serum of NPC mice to a similar extent to those obtained by genetic Npc1 rescue of liver cells. Gadolinium salts are widely used as magnetic resonance imaging (MRI) contrasts. This study opens the possibility of targeting foam cells with Gadolinium or by other means for improving NPC liver disease. Synopsis: Gadolinium Chloride can effectively rescue some parameters of liver dysfunction in NPC mice and its potential use in patients should be carefully evaluated.
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Gadolinium Chloride rescues niemann pick type c liver damage
International Journal of Molecular Sciences, 2018Co-Authors: Andrés D. Klein, Juan Esteban Oyarzún, Cristian Cortez, Silvana ZanlungoAbstract:Niemann–Pick type C (NPC) disease is a rare neurovisceral cholesterol storage disorder that arises from loss of function mutations in the NPC1 or NPC2 genes. Soon after birth, some patients present with an aggressive hepatosplenomegaly and cholestatic signs. Histopathologically, the liver presents with large numbers of foam cells; however, their role in disease pathogenesis has not been explored in depth. Here, we studied the consequences of Gadolinium Chloride (GdCl3) treatment, a well-known Kupffer/foam cell inhibitor, at late stages of NPC liver disease and compared it with NPC1 genetic rescue in hepatocytes in vivo. GdCl3 treatment successfully blocked the endocytic capacity of hepatic Kupffer/foam measured by India ink endocytosis, decreased the levels CD68—A marker of Kupffer cells in the liver—and normalized the transaminase levels in serum of NPC mice to a similar extent to those obtained by genetic Npc1 rescue of liver cells. Gadolinium salts are widely used as magnetic resonance imaging (MRI) contrasts. This study opens the possibility of targeting foam cells with Gadolinium or by other means for improving NPC liver disease. Synopsis: Gadolinium Chloride can effectively rescue some parameters of liver dysfunction in NPC mice and its potential use in patients should be carefully evaluated.
Juan Esteban Oyarzún - One of the best experts on this subject based on the ideXlab platform.
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Gadolinium Chloride Rescues Niemann–Pick Type C Liver Damage
International journal of molecular sciences, 2018Co-Authors: Andrés D. Klein, Juan Esteban Oyarzún, Cristian Cortez, Silvana ZanlungoAbstract:Niemann–Pick type C (NPC) disease is a rare neurovisceral cholesterol storage disorder that arises from loss of function mutations in the NPC1 or NPC2 genes. Soon after birth, some patients present with an aggressive hepatosplenomegaly and cholestatic signs. Histopathologically, the liver presents with large numbers of foam cells; however, their role in disease pathogenesis has not been explored in depth. Here, we studied the consequences of Gadolinium Chloride (GdCl3) treatment, a well-known Kupffer/foam cell inhibitor, at late stages of NPC liver disease and compared it with NPC1 genetic rescue in hepatocytes in vivo. GdCl3 treatment successfully blocked the endocytic capacity of hepatic Kupffer/foam measured by India ink endocytosis, decreased the levels CD68—A marker of Kupffer cells in the liver—and normalized the transaminase levels in serum of NPC mice to a similar extent to those obtained by genetic Npc1 rescue of liver cells. Gadolinium salts are widely used as magnetic resonance imaging (MRI) contrasts. This study opens the possibility of targeting foam cells with Gadolinium or by other means for improving NPC liver disease. Synopsis: Gadolinium Chloride can effectively rescue some parameters of liver dysfunction in NPC mice and its potential use in patients should be carefully evaluated.
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Gadolinium Chloride rescues niemann pick type c liver damage
International Journal of Molecular Sciences, 2018Co-Authors: Andrés D. Klein, Juan Esteban Oyarzún, Cristian Cortez, Silvana ZanlungoAbstract:Niemann–Pick type C (NPC) disease is a rare neurovisceral cholesterol storage disorder that arises from loss of function mutations in the NPC1 or NPC2 genes. Soon after birth, some patients present with an aggressive hepatosplenomegaly and cholestatic signs. Histopathologically, the liver presents with large numbers of foam cells; however, their role in disease pathogenesis has not been explored in depth. Here, we studied the consequences of Gadolinium Chloride (GdCl3) treatment, a well-known Kupffer/foam cell inhibitor, at late stages of NPC liver disease and compared it with NPC1 genetic rescue in hepatocytes in vivo. GdCl3 treatment successfully blocked the endocytic capacity of hepatic Kupffer/foam measured by India ink endocytosis, decreased the levels CD68—A marker of Kupffer cells in the liver—and normalized the transaminase levels in serum of NPC mice to a similar extent to those obtained by genetic Npc1 rescue of liver cells. Gadolinium salts are widely used as magnetic resonance imaging (MRI) contrasts. This study opens the possibility of targeting foam cells with Gadolinium or by other means for improving NPC liver disease. Synopsis: Gadolinium Chloride can effectively rescue some parameters of liver dysfunction in NPC mice and its potential use in patients should be carefully evaluated.
Hirofumi Fujii - One of the best experts on this subject based on the ideXlab platform.
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Delayed hepatic signal recovery on ferucarbotran-enhanced magnetic resonance images: an experimental study in rat livers with Gadolinium Chloride-induced Kupffer cell damage
Magnetic Resonance Materials in Physics Biology and Medicine, 2013Co-Authors: Toshihiro Furuta, Masaaki Akahane, Kuni Ohtomo, Masayuki Yamaguchi, Manabu Minami, Ryutaro Nakagami, Noriyuki Moriyama, Hirofumi FujiiAbstract:Objective Hepatic signal recovery, rather than reduction, in ferucarbotran-enhanced magnetic resonance imaging (MRI) is a potential diagnostic marker of liver damage. We investigated hepatic signal recovery in rats with Gadolinium Chloride (GdCl_3)-induced Kupffer cell (KC) damage. Materials and methods Twelve rats received 8 μmol iron/kg of ferucarbotran 1 day after 0–7.5 mg/kg GdCl_3 injection (experiment A). Another 12 rats received ferucarbotran followed by GdCl_3 injection 6 h later (experiment B). In each experiment, three rats without GdCl_3 (“no injury group”) served as control. Another six rats received GdCl_3 alone without ferucarbotran. Hepatic signals were assessed on T _2 ^* -weighted images for up to 29 days. Iron deposits were histologically examined on day 29. Results Hepatic signal recovery was delayed in a GdCl_3 dose-dependent manner in experiment A. Gadolinium Chloride alone reduced hepatic signal 15 % during this experiment. Hepatic signal recovery was delayed only in rats that received 7.5 mg/kg GdCl_3 in experiment B. Hepatic signals negatively correlated with iron deposits in KCs and hepatocytes. Conclusion Hepatic signal recovery on ferucarbotran-enhanced MRI was delayed in the context of GdCl_3-induced KC damage due to increased hepatic iron deposits. Hepatic signal recovery may be used as a clinical marker of KC damage in liver disorders, including radiation-induced hepatitis.
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Delayed hepatic signal recovery on ferucarbotran-enhanced magnetic resonance images: an experimental study in rat livers with Gadolinium Chloride-induced Kupffer cell damage
Magma (New York N.Y.), 2012Co-Authors: Toshihiro Furuta, Masaaki Akahane, Kuni Ohtomo, Masayuki Yamaguchi, Manabu Minami, Ryutaro Nakagami, Noriyuki Moriyama, Hirofumi FujiiAbstract:Objective Hepatic signal recovery, rather than reduction, in ferucarbotran-enhanced magnetic resonance imaging (MRI) is a potential diagnostic marker of liver damage. We investigated hepatic signal recovery in rats with Gadolinium Chloride (GdCl3)-induced Kupffer cell (KC) damage.