The Experts below are selected from a list of 1182 Experts worldwide ranked by ideXlab platform
Kaeko Kamei - One of the best experts on this subject based on the ideXlab platform.
-
8 The Potential of Galangal (Alpinia galanga Linn.) Extract against the Pathogens that Cause White Feces Syndrome and Acute Hepatopancreatic Necrosis Disease (AHPND) in Pacific White Shrimp (Litopenaeus vannamei)
2016Co-Authors: Tidaporn Chaweepack, Surachart Chaweepack, Boonyee Muenthaisong, Kaeko KameiAbstract:White feces syndrome and acute hepatopancreatic necrosis disease (AHPND) are serious diseases that have recently been noted in Pacific white shrimp (Litopenaeus vannamei). Vibrio bacteria and 6 species of fungi (Aspergillus flavus, A. ochraceus, A. japonicus, Penicillium sp., Fusarium sp., and Cladosporium cladosporioides) were isolated from shrimp naturally infected with white feces syndrome. Antibiotics have been used to treatment the disease for many years, but these have been ineffective and have resulted in drug residue problems for the shrimp industry. In this study, an alternative method was tested for its efficacy in controlling these pathogens. The crude extract of Galangal (Alpinia galanga Linn.), an herbal medicine, inhibited the growth of 8 vibrio species of the pathogen, V. parahaemolyticus (EMS/AHPND) in particular. The results also showed that 0.5 mg/ml of the Galangal extract was a concentration that produced the strongest inhibition of the fungi A. ochraceus. Naturally infested shrimp L. vannamei were fed 2 and 4 % (v/w) portions of the herb extract for 12 days and their progress was compared with that of a control group (no herb extract). At the end of the feeding trial, the numbers of total Vibrio spp. and the incidence of fungi infestation in the hepatopancreas and intestines of treated shrimp were significantl
-
the potential of Galangal alpinia galanga linn extract against the pathogens that cause white feces syndrome and acute hepatopancreatic necrosis disease ahpnd in pacific white shrimp litopenaeus vannamei
International Journal of Biology, 2015Co-Authors: Tidaporn Chaweepack, Surachart Chaweepack, Boonyee Muenthaisong, Kaeko KameiAbstract:White feces syndrome and acute hepatopancreatic necrosis disease (AHPND) are serious diseases that have recently been noted in Pacific white shrimp (Litopenaeus vannamei). Vibrio bacteria and 6 species of fungi (Aspergillus flavus, A. ochraceus, A. japonicus, Penicillium sp., Fusarium sp., and Cladosporium cladosporioides) were isolated from shrimp naturally infected with white feces syndrome. Antibiotics have been used to treatment the disease for many years, but these have been ineffective and have resulted in drug residue problems for the shrimp industry. In this study, an alternative method was tested for its efficacy in controlling these pathogens. The crude extract of Galangal (Alpinia galanga Linn.), an herbal medicine, inhibited the growth of 8 vibrio species of the pathogen, V. parahaemolyticus (EMS/AHPND) in particular. The results also showed that 0.5 mg/ml of the Galangal extract was a concentration that produced the strongest inhibition of the fungi A. ochraceus. Naturally infested shrimp L. vannamei were fed 2 and 4% (v/w) portions of the herb extract for 12 days and their progress was compared with that of a control group (no herb extract). At the end of the feeding trial, the numbers of total Vibrio spp. and the incidence of fungi infestation in the hepatopancreas and intestines of treated shrimp were significantly lower than that in the control group (P<0.05). Furthermore, the survival rates for the treatment groups, after injections with V. parahaemolyticus (EMS/AHPND), were significantly higher than that of the control group (P<0.05). Based on these results, we can report that the Galangal extract has antimicrobial properties that are applicable as bio-medicinal agents against white feces syndrome and AHPND. Therefore, in the future this herb should be an alternative to chemotherapeutic agents that are being used in the shrimp industry.
-
Effect of Galangal (Alpinia galanga Linn.) extract on the expression of immune-related genes and Vibrio harveyi resistance in Pacific white shrimp (Litopenaeus vannamei)
Aquaculture International, 2015Co-Authors: Tidaporn Chaweepack, Surachart Chaweepack, Boonyee Muenthaisong, Lila Ruangpan, Kei Nagata, Kaeko KameiAbstract:Some plant and herb extracts reportedly possess antimicrobial activities and also have the ability to enhance the nonspecific immune system of shrimp, thereby promoting disease resistance. In this study, a natural herb, Galangal ( Alpinia galanga Linn.), was used to prevent infectious disease in cultured shrimp, and the effects of Galangal–ethanol extract on the expression of the immune-related genes in the shrimp were analyzed via a reverse-transcription polymerase chain reaction of mRNA in circulating hemocytes. Following the intramuscular injection of either Galangal extract or trans - p -coumaryl diacetate that had been isolated from Galangal rhizome, Pacific white shrimp ( Litopenaeus vannamei ) showed significant increases in the relative expression ratio of the six immune-related genes compared with a control group. Furthermore, following the oral administration of Galangal extract, similar inducible effects of the expression of immune-related genes in the Pacific white shrimp were obtained, which led to an enhanced survival rate from Vibrio harveyi infection. Thus, this study revealed that both Galangal extract and trans - p -coumaryl diacetate stimulated the immune system response, thereby promoting resistance to V. harveyi infection in Pacific white shrimp.
Tidaporn Chaweepack - One of the best experts on this subject based on the ideXlab platform.
-
8 The Potential of Galangal (Alpinia galanga Linn.) Extract against the Pathogens that Cause White Feces Syndrome and Acute Hepatopancreatic Necrosis Disease (AHPND) in Pacific White Shrimp (Litopenaeus vannamei)
2016Co-Authors: Tidaporn Chaweepack, Surachart Chaweepack, Boonyee Muenthaisong, Kaeko KameiAbstract:White feces syndrome and acute hepatopancreatic necrosis disease (AHPND) are serious diseases that have recently been noted in Pacific white shrimp (Litopenaeus vannamei). Vibrio bacteria and 6 species of fungi (Aspergillus flavus, A. ochraceus, A. japonicus, Penicillium sp., Fusarium sp., and Cladosporium cladosporioides) were isolated from shrimp naturally infected with white feces syndrome. Antibiotics have been used to treatment the disease for many years, but these have been ineffective and have resulted in drug residue problems for the shrimp industry. In this study, an alternative method was tested for its efficacy in controlling these pathogens. The crude extract of Galangal (Alpinia galanga Linn.), an herbal medicine, inhibited the growth of 8 vibrio species of the pathogen, V. parahaemolyticus (EMS/AHPND) in particular. The results also showed that 0.5 mg/ml of the Galangal extract was a concentration that produced the strongest inhibition of the fungi A. ochraceus. Naturally infested shrimp L. vannamei were fed 2 and 4 % (v/w) portions of the herb extract for 12 days and their progress was compared with that of a control group (no herb extract). At the end of the feeding trial, the numbers of total Vibrio spp. and the incidence of fungi infestation in the hepatopancreas and intestines of treated shrimp were significantl
-
the potential of Galangal alpinia galanga linn extract against the pathogens that cause white feces syndrome and acute hepatopancreatic necrosis disease ahpnd in pacific white shrimp litopenaeus vannamei
International Journal of Biology, 2015Co-Authors: Tidaporn Chaweepack, Surachart Chaweepack, Boonyee Muenthaisong, Kaeko KameiAbstract:White feces syndrome and acute hepatopancreatic necrosis disease (AHPND) are serious diseases that have recently been noted in Pacific white shrimp (Litopenaeus vannamei). Vibrio bacteria and 6 species of fungi (Aspergillus flavus, A. ochraceus, A. japonicus, Penicillium sp., Fusarium sp., and Cladosporium cladosporioides) were isolated from shrimp naturally infected with white feces syndrome. Antibiotics have been used to treatment the disease for many years, but these have been ineffective and have resulted in drug residue problems for the shrimp industry. In this study, an alternative method was tested for its efficacy in controlling these pathogens. The crude extract of Galangal (Alpinia galanga Linn.), an herbal medicine, inhibited the growth of 8 vibrio species of the pathogen, V. parahaemolyticus (EMS/AHPND) in particular. The results also showed that 0.5 mg/ml of the Galangal extract was a concentration that produced the strongest inhibition of the fungi A. ochraceus. Naturally infested shrimp L. vannamei were fed 2 and 4% (v/w) portions of the herb extract for 12 days and their progress was compared with that of a control group (no herb extract). At the end of the feeding trial, the numbers of total Vibrio spp. and the incidence of fungi infestation in the hepatopancreas and intestines of treated shrimp were significantly lower than that in the control group (P<0.05). Furthermore, the survival rates for the treatment groups, after injections with V. parahaemolyticus (EMS/AHPND), were significantly higher than that of the control group (P<0.05). Based on these results, we can report that the Galangal extract has antimicrobial properties that are applicable as bio-medicinal agents against white feces syndrome and AHPND. Therefore, in the future this herb should be an alternative to chemotherapeutic agents that are being used in the shrimp industry.
-
Effect of Galangal (Alpinia galanga Linn.) extract on the expression of immune-related genes and Vibrio harveyi resistance in Pacific white shrimp (Litopenaeus vannamei)
Aquaculture International, 2015Co-Authors: Tidaporn Chaweepack, Surachart Chaweepack, Boonyee Muenthaisong, Lila Ruangpan, Kei Nagata, Kaeko KameiAbstract:Some plant and herb extracts reportedly possess antimicrobial activities and also have the ability to enhance the nonspecific immune system of shrimp, thereby promoting disease resistance. In this study, a natural herb, Galangal ( Alpinia galanga Linn.), was used to prevent infectious disease in cultured shrimp, and the effects of Galangal–ethanol extract on the expression of the immune-related genes in the shrimp were analyzed via a reverse-transcription polymerase chain reaction of mRNA in circulating hemocytes. Following the intramuscular injection of either Galangal extract or trans - p -coumaryl diacetate that had been isolated from Galangal rhizome, Pacific white shrimp ( Litopenaeus vannamei ) showed significant increases in the relative expression ratio of the six immune-related genes compared with a control group. Furthermore, following the oral administration of Galangal extract, similar inducible effects of the expression of immune-related genes in the Pacific white shrimp were obtained, which led to an enhanced survival rate from Vibrio harveyi infection. Thus, this study revealed that both Galangal extract and trans - p -coumaryl diacetate stimulated the immune system response, thereby promoting resistance to V. harveyi infection in Pacific white shrimp.
Boonyee Muenthaisong - One of the best experts on this subject based on the ideXlab platform.
-
8 The Potential of Galangal (Alpinia galanga Linn.) Extract against the Pathogens that Cause White Feces Syndrome and Acute Hepatopancreatic Necrosis Disease (AHPND) in Pacific White Shrimp (Litopenaeus vannamei)
2016Co-Authors: Tidaporn Chaweepack, Surachart Chaweepack, Boonyee Muenthaisong, Kaeko KameiAbstract:White feces syndrome and acute hepatopancreatic necrosis disease (AHPND) are serious diseases that have recently been noted in Pacific white shrimp (Litopenaeus vannamei). Vibrio bacteria and 6 species of fungi (Aspergillus flavus, A. ochraceus, A. japonicus, Penicillium sp., Fusarium sp., and Cladosporium cladosporioides) were isolated from shrimp naturally infected with white feces syndrome. Antibiotics have been used to treatment the disease for many years, but these have been ineffective and have resulted in drug residue problems for the shrimp industry. In this study, an alternative method was tested for its efficacy in controlling these pathogens. The crude extract of Galangal (Alpinia galanga Linn.), an herbal medicine, inhibited the growth of 8 vibrio species of the pathogen, V. parahaemolyticus (EMS/AHPND) in particular. The results also showed that 0.5 mg/ml of the Galangal extract was a concentration that produced the strongest inhibition of the fungi A. ochraceus. Naturally infested shrimp L. vannamei were fed 2 and 4 % (v/w) portions of the herb extract for 12 days and their progress was compared with that of a control group (no herb extract). At the end of the feeding trial, the numbers of total Vibrio spp. and the incidence of fungi infestation in the hepatopancreas and intestines of treated shrimp were significantl
-
the potential of Galangal alpinia galanga linn extract against the pathogens that cause white feces syndrome and acute hepatopancreatic necrosis disease ahpnd in pacific white shrimp litopenaeus vannamei
International Journal of Biology, 2015Co-Authors: Tidaporn Chaweepack, Surachart Chaweepack, Boonyee Muenthaisong, Kaeko KameiAbstract:White feces syndrome and acute hepatopancreatic necrosis disease (AHPND) are serious diseases that have recently been noted in Pacific white shrimp (Litopenaeus vannamei). Vibrio bacteria and 6 species of fungi (Aspergillus flavus, A. ochraceus, A. japonicus, Penicillium sp., Fusarium sp., and Cladosporium cladosporioides) were isolated from shrimp naturally infected with white feces syndrome. Antibiotics have been used to treatment the disease for many years, but these have been ineffective and have resulted in drug residue problems for the shrimp industry. In this study, an alternative method was tested for its efficacy in controlling these pathogens. The crude extract of Galangal (Alpinia galanga Linn.), an herbal medicine, inhibited the growth of 8 vibrio species of the pathogen, V. parahaemolyticus (EMS/AHPND) in particular. The results also showed that 0.5 mg/ml of the Galangal extract was a concentration that produced the strongest inhibition of the fungi A. ochraceus. Naturally infested shrimp L. vannamei were fed 2 and 4% (v/w) portions of the herb extract for 12 days and their progress was compared with that of a control group (no herb extract). At the end of the feeding trial, the numbers of total Vibrio spp. and the incidence of fungi infestation in the hepatopancreas and intestines of treated shrimp were significantly lower than that in the control group (P<0.05). Furthermore, the survival rates for the treatment groups, after injections with V. parahaemolyticus (EMS/AHPND), were significantly higher than that of the control group (P<0.05). Based on these results, we can report that the Galangal extract has antimicrobial properties that are applicable as bio-medicinal agents against white feces syndrome and AHPND. Therefore, in the future this herb should be an alternative to chemotherapeutic agents that are being used in the shrimp industry.
-
Effect of Galangal (Alpinia galanga Linn.) extract on the expression of immune-related genes and Vibrio harveyi resistance in Pacific white shrimp (Litopenaeus vannamei)
Aquaculture International, 2015Co-Authors: Tidaporn Chaweepack, Surachart Chaweepack, Boonyee Muenthaisong, Lila Ruangpan, Kei Nagata, Kaeko KameiAbstract:Some plant and herb extracts reportedly possess antimicrobial activities and also have the ability to enhance the nonspecific immune system of shrimp, thereby promoting disease resistance. In this study, a natural herb, Galangal ( Alpinia galanga Linn.), was used to prevent infectious disease in cultured shrimp, and the effects of Galangal–ethanol extract on the expression of the immune-related genes in the shrimp were analyzed via a reverse-transcription polymerase chain reaction of mRNA in circulating hemocytes. Following the intramuscular injection of either Galangal extract or trans - p -coumaryl diacetate that had been isolated from Galangal rhizome, Pacific white shrimp ( Litopenaeus vannamei ) showed significant increases in the relative expression ratio of the six immune-related genes compared with a control group. Furthermore, following the oral administration of Galangal extract, similar inducible effects of the expression of immune-related genes in the Pacific white shrimp were obtained, which led to an enhanced survival rate from Vibrio harveyi infection. Thus, this study revealed that both Galangal extract and trans - p -coumaryl diacetate stimulated the immune system response, thereby promoting resistance to V. harveyi infection in Pacific white shrimp.
Surachart Chaweepack - One of the best experts on this subject based on the ideXlab platform.
-
8 The Potential of Galangal (Alpinia galanga Linn.) Extract against the Pathogens that Cause White Feces Syndrome and Acute Hepatopancreatic Necrosis Disease (AHPND) in Pacific White Shrimp (Litopenaeus vannamei)
2016Co-Authors: Tidaporn Chaweepack, Surachart Chaweepack, Boonyee Muenthaisong, Kaeko KameiAbstract:White feces syndrome and acute hepatopancreatic necrosis disease (AHPND) are serious diseases that have recently been noted in Pacific white shrimp (Litopenaeus vannamei). Vibrio bacteria and 6 species of fungi (Aspergillus flavus, A. ochraceus, A. japonicus, Penicillium sp., Fusarium sp., and Cladosporium cladosporioides) were isolated from shrimp naturally infected with white feces syndrome. Antibiotics have been used to treatment the disease for many years, but these have been ineffective and have resulted in drug residue problems for the shrimp industry. In this study, an alternative method was tested for its efficacy in controlling these pathogens. The crude extract of Galangal (Alpinia galanga Linn.), an herbal medicine, inhibited the growth of 8 vibrio species of the pathogen, V. parahaemolyticus (EMS/AHPND) in particular. The results also showed that 0.5 mg/ml of the Galangal extract was a concentration that produced the strongest inhibition of the fungi A. ochraceus. Naturally infested shrimp L. vannamei were fed 2 and 4 % (v/w) portions of the herb extract for 12 days and their progress was compared with that of a control group (no herb extract). At the end of the feeding trial, the numbers of total Vibrio spp. and the incidence of fungi infestation in the hepatopancreas and intestines of treated shrimp were significantl
-
the potential of Galangal alpinia galanga linn extract against the pathogens that cause white feces syndrome and acute hepatopancreatic necrosis disease ahpnd in pacific white shrimp litopenaeus vannamei
International Journal of Biology, 2015Co-Authors: Tidaporn Chaweepack, Surachart Chaweepack, Boonyee Muenthaisong, Kaeko KameiAbstract:White feces syndrome and acute hepatopancreatic necrosis disease (AHPND) are serious diseases that have recently been noted in Pacific white shrimp (Litopenaeus vannamei). Vibrio bacteria and 6 species of fungi (Aspergillus flavus, A. ochraceus, A. japonicus, Penicillium sp., Fusarium sp., and Cladosporium cladosporioides) were isolated from shrimp naturally infected with white feces syndrome. Antibiotics have been used to treatment the disease for many years, but these have been ineffective and have resulted in drug residue problems for the shrimp industry. In this study, an alternative method was tested for its efficacy in controlling these pathogens. The crude extract of Galangal (Alpinia galanga Linn.), an herbal medicine, inhibited the growth of 8 vibrio species of the pathogen, V. parahaemolyticus (EMS/AHPND) in particular. The results also showed that 0.5 mg/ml of the Galangal extract was a concentration that produced the strongest inhibition of the fungi A. ochraceus. Naturally infested shrimp L. vannamei were fed 2 and 4% (v/w) portions of the herb extract for 12 days and their progress was compared with that of a control group (no herb extract). At the end of the feeding trial, the numbers of total Vibrio spp. and the incidence of fungi infestation in the hepatopancreas and intestines of treated shrimp were significantly lower than that in the control group (P<0.05). Furthermore, the survival rates for the treatment groups, after injections with V. parahaemolyticus (EMS/AHPND), were significantly higher than that of the control group (P<0.05). Based on these results, we can report that the Galangal extract has antimicrobial properties that are applicable as bio-medicinal agents against white feces syndrome and AHPND. Therefore, in the future this herb should be an alternative to chemotherapeutic agents that are being used in the shrimp industry.
-
Effect of Galangal (Alpinia galanga Linn.) extract on the expression of immune-related genes and Vibrio harveyi resistance in Pacific white shrimp (Litopenaeus vannamei)
Aquaculture International, 2015Co-Authors: Tidaporn Chaweepack, Surachart Chaweepack, Boonyee Muenthaisong, Lila Ruangpan, Kei Nagata, Kaeko KameiAbstract:Some plant and herb extracts reportedly possess antimicrobial activities and also have the ability to enhance the nonspecific immune system of shrimp, thereby promoting disease resistance. In this study, a natural herb, Galangal ( Alpinia galanga Linn.), was used to prevent infectious disease in cultured shrimp, and the effects of Galangal–ethanol extract on the expression of the immune-related genes in the shrimp were analyzed via a reverse-transcription polymerase chain reaction of mRNA in circulating hemocytes. Following the intramuscular injection of either Galangal extract or trans - p -coumaryl diacetate that had been isolated from Galangal rhizome, Pacific white shrimp ( Litopenaeus vannamei ) showed significant increases in the relative expression ratio of the six immune-related genes compared with a control group. Furthermore, following the oral administration of Galangal extract, similar inducible effects of the expression of immune-related genes in the Pacific white shrimp were obtained, which led to an enhanced survival rate from Vibrio harveyi infection. Thus, this study revealed that both Galangal extract and trans - p -coumaryl diacetate stimulated the immune system response, thereby promoting resistance to V. harveyi infection in Pacific white shrimp.
-, Azis Saifudin, Ph.d. Apt - One of the best experts on this subject based on the ideXlab platform.
-
Efek Sinergisme Fraksi Etil Asetat Rimpang Lengkuas Dengan Fraksi Etil Asetat Buah Kemukus, Kulit Kayumanis, Daun Jeruk Purut Dan Biji Pinang Terhadap Sel Kanker T47d
2019Co-Authors: Suharjo, Delta Nugraheni, -, Azis Saifudin, Ph.d. AptAbstract:Galangal (Alpinia galanga (L.) Wild.) is a plant that has been studied as an anticancer agent. Other plants that also have cytotoxic activity are kemukus (Piper cubeba L.f.), cinnamon (Cinnamomum zeylanicum), kaffir lime (Citrus hystrix DC.) and areca nut (Areca catechu L.). The study was conducted to determine the effect of synergism between Galangal and the four plants on T47D cells. The study was conducted by extraction of simplicia for 3 days. Then the fractionation was carried out with ethyl acetate : aquades. Cytotoxic activity was tested by using MTT assay. The synergistic effect is indicated by comparing of IC50 values between single and combination sample. The results showed that the combination of Galangal with kemukus, cinnamon, kaffir lime and areca nut does not showed sinergism effect. The cytotoxic activity of the four plants against T47D cancer cells increased with combination with Galangal. The IC50 value of single Galangal is 60.96 µg/mL while the IC50 value of Galangal is a combination of Galangal; Galangal-cinnamon; Galangal kaffir lime and areca nut respectively 98.88; 83.24; 69.30 and 85.56 μg/mL
-
Sinergisme Fraksi Etil Asetat Rimpang Lengkuas Dengan Umbi Bawang Dayak, Kulit Manggis, Biji Mahoni, dan Rimpang Jahe Pada Sel T47D
2019Co-Authors: Brigita, Ririn Ulpha, -, Azis Saifudin, Ph.d. AptAbstract:Galangal (Alpinia galanga (L.) Willd.) is one of the natural ingredients that has the potential to be developed as an anticancer drug. In addition to Galangal, other plants that are also reported to have anticancer activities are dayak onions (Eleutherine bulbosa (Mill.) Urb.), mangosteen (Garcinia mangostana (L.)), mahogany (Swietenia mahagoni (L.) Jacq.), and ginger (Zingiber officinale Roscoe.). This study aimed to determine the synergism of the four plants ethyl acetate fraction with Galangal rhizome ethyl acetate fraction in T47D cells. The extract was obtained by maceration method using methanol solvent then fractionation was carried out using ethyl acetate and distilled water solvents to obtain ethyl acetate fraction. Cytotoxic activity was tested by using MTT method and read using an ELISA reader at a wavelength of 550 nm. The results showed that a single Galangal rhizome test and a combination test with four plants gave cytotoxic activity which was equally moderate to T47D cells, so it could be concluded that the combination of four plants with Galangal rhizomes showed an additive effect. The result showed the combination of Galangal-mangosteen rhizome rhizome had stronger cytotoxic activity (IC50 41,860 µg/mL) compared to single Galangal rhizome (IC50 65,383 µg/mL)
