The Experts below are selected from a list of 96 Experts worldwide ranked by ideXlab platform
Alireza Abbasi - One of the best experts on this subject based on the ideXlab platform.
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Transcriptome and metabolome analysis of Ferula gummosa Boiss. to reveal major biosynthetic pathways of Galbanum compounds
Functional & Integrative Genomics, 2017Co-Authors: Ahmad Sobhani Najafabadi, Mohammad Reza Naghavi, Hamid Farahmand, Alireza AbbasiAbstract:Ferula gummosa Boiss. is an industrial and pharmaceutical plant that has been highly recognized for its valuable oleo-gum-resin, namely Galbanum. Despite the fabulous value of Galbanum, very little information on the genetic and biochemical mechanisms of its production existed. In the present study, the oleo-gum-resin and four organs (root, flower, stem, and leaf) of F. gummosa were assessed in terms of metabolic compositions and the expression of genes involved in their biosynthetic pathways. Results showed that the most accumulation of resin and essential oils were occurred in the roots (13.99 mg/g) and flowers (6.01 mg/g), respectively. While the most dominant compound of the resin was β-amyrin from triterpenes, the most abundant compounds of the essential oils were α-pinene and β-pinene from monoterpenes and α-eudesmol and germacrene-D from sesquiterpenes. Transcriptome analysis was performed by RNA sequencing (RNA-seq) for the plant roots and flowers. Differential gene expression analysis showed that 1172 unigenes were differential between two organs that 934 (79.6%) of them were up-regulated in the flowers and 238 (20.4%) unigenes were up-regulated in the roots (FDR ≤0.001). The most important up-regulated unigenes in the roots were involved in the biosynthesis of the major components of Galbanum, including myrcene, germacrene-D, α-terpineol, and β-amyrin. The results obtained by RNA-Seq were confirmed by qPCR. These analyses showed that different organs of F. gummosa are involved in the production of oleo-gum-resin, but the roots are more active than other organs in terms of the biosynthesis of triterpenes and some mono- and sesquiterpenes. This study provides rich molecular and biochemical resources for further studies on molecular genetics and functional genomics of oleo-gum-resin production in F. gummosa .
Milad Iranshahy - One of the best experts on this subject based on the ideXlab platform.
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metabolic differences of two ferula species as potential sources of Galbanum an nmr based metabolomics study
Phytochemical Analysis, 2021Co-Authors: Faegheh Farhadi, Mehrdad Iranshahi, Leila Mohtashami, Shokrollah Shakeri Asil, Milad IranshahyAbstract:INTRODUCTION Ferula gummosa Boiss. and Ferula galbaniflua Boiss. & Buhse (Apiaceae) are two important Iranian plants that are considered as potential sources of Galbanum (barijeh). Galbanum is traditionally used for treating different diseases including flatulence and memory impairment. OBJECTIVE According to a phylogenetic analysis of the nrDNA ITS sequence and the Flora Iranica, F. gummosa has been considered as a synonym of F. galbaniflua. However, F. galbaniflua and F. gummosa grow in two different geographical locations and have different metabolic patterns. Some researchers believe that F. gummosa and F. galbaniflua are two distinct species. To discriminate these species, we compared metabolic profiles of F. gummosa and F. galbaniflua samples. METHODS 1 H-NMR-based metabolomics analysis was used for classification of F. gummosa and F. galbaniflua samples collected from northeast Iran. The acquired data were analyzed using hierarchical cluster analysis (HCA), partial least squares discriminant analysis (PLS-DA) and orthogonal projection to latent structures discriminant analysis (OPLS-DA). RESULTS The result showed a clear separation between the two species that may be related to the quantity and diversity of their metabolites. Ferula gummosa had higher mogoltacin levels, while F. galbaniflua had higher feselol levels. Ligupersin A and conferdione were significantly detected in F. gummosa, whereas sterol compounds were significantly detected in F. galbaniflua. CONCLUSION Our findings indicate that clear metabolomics discrimination of F. gummosa and F. galbaniflua makes their chemotaxonomic classification possible.
Ahmad Sobhani Najafabadi - One of the best experts on this subject based on the ideXlab platform.
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Transcriptome and metabolome analysis of Ferula gummosa Boiss. to reveal major biosynthetic pathways of Galbanum compounds
Functional & Integrative Genomics, 2017Co-Authors: Ahmad Sobhani Najafabadi, Mohammad Reza Naghavi, Hamid Farahmand, Alireza AbbasiAbstract:Ferula gummosa Boiss. is an industrial and pharmaceutical plant that has been highly recognized for its valuable oleo-gum-resin, namely Galbanum. Despite the fabulous value of Galbanum, very little information on the genetic and biochemical mechanisms of its production existed. In the present study, the oleo-gum-resin and four organs (root, flower, stem, and leaf) of F. gummosa were assessed in terms of metabolic compositions and the expression of genes involved in their biosynthetic pathways. Results showed that the most accumulation of resin and essential oils were occurred in the roots (13.99 mg/g) and flowers (6.01 mg/g), respectively. While the most dominant compound of the resin was β-amyrin from triterpenes, the most abundant compounds of the essential oils were α-pinene and β-pinene from monoterpenes and α-eudesmol and germacrene-D from sesquiterpenes. Transcriptome analysis was performed by RNA sequencing (RNA-seq) for the plant roots and flowers. Differential gene expression analysis showed that 1172 unigenes were differential between two organs that 934 (79.6%) of them were up-regulated in the flowers and 238 (20.4%) unigenes were up-regulated in the roots (FDR ≤0.001). The most important up-regulated unigenes in the roots were involved in the biosynthesis of the major components of Galbanum, including myrcene, germacrene-D, α-terpineol, and β-amyrin. The results obtained by RNA-Seq were confirmed by qPCR. These analyses showed that different organs of F. gummosa are involved in the production of oleo-gum-resin, but the roots are more active than other organs in terms of the biosynthesis of triterpenes and some mono- and sesquiterpenes. This study provides rich molecular and biochemical resources for further studies on molecular genetics and functional genomics of oleo-gum-resin production in F. gummosa .
Maryam Fahimdanesh - One of the best experts on this subject based on the ideXlab platform.
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a novel bioactive edible coating based on sodium alginate and Galbanum gum incorporated with essential oil of ziziphora persica the antioxidant and antimicrobial activity and application in food model
Food Hydrocolloids, 2017Co-Authors: Hassan Hamedi, Mina Kargozari, Peyman Mahasti Shotorbani, Nima Babolani Mogadam, Maryam FahimdaneshAbstract:Abstract Galbanum, an aromatic oleo-resin gum, is produced from umbelliferous Persian plant species in the genus Ferula with the common Persian name “Barije”, chiefly endemic flora of the mountain ranges of northern Iran. Ziziphora persica is an edible medicinal plant, which is widely distributed in Iran, Turkey and Eurasian countries. The aims of the present research were to produce novel bioactive edible coatings based on sodium alginate (Alg), Galbanum oleo-resin gum (GG) and the biocomposite of alginate and Galbanum (CAG) containing different concentration of Ziziphora essential oil (ZEO), and evaluate the antioxidant and the antimicrobial activities of these substances in vitro and their effect on the quality and shelf life of chicken fillet during cold storage. Overall, the MIC and MBC values of GG and ZEO extracts ranging from 1.25 to 50 (μg mL −1 ) proved that gram-positive bacteria were more susceptible to GG and ZEO than gram-negative bacteria. The high phenolic and flavonoid contents and antioxidant activities of GG and ZEO were proved by in vitro analysis. Results showed that coatings of Alg had no significant effect on decreasing the microbial load of aerobic mesophilic and psychrotrophic bacteria, lactic acid bacteria, Pseudomonas spp., and Enterobacteriaceae , as well as L. monocytogenes (p > 0.05), while the coating of fillet with GG, CAG alone and in combination with ZEO showed a significant differences with the other treatments during 12 days of storage (p
Faegheh Farhadi - One of the best experts on this subject based on the ideXlab platform.
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metabolic differences of two ferula species as potential sources of Galbanum an nmr based metabolomics study
Phytochemical Analysis, 2021Co-Authors: Faegheh Farhadi, Mehrdad Iranshahi, Leila Mohtashami, Shokrollah Shakeri Asil, Milad IranshahyAbstract:INTRODUCTION Ferula gummosa Boiss. and Ferula galbaniflua Boiss. & Buhse (Apiaceae) are two important Iranian plants that are considered as potential sources of Galbanum (barijeh). Galbanum is traditionally used for treating different diseases including flatulence and memory impairment. OBJECTIVE According to a phylogenetic analysis of the nrDNA ITS sequence and the Flora Iranica, F. gummosa has been considered as a synonym of F. galbaniflua. However, F. galbaniflua and F. gummosa grow in two different geographical locations and have different metabolic patterns. Some researchers believe that F. gummosa and F. galbaniflua are two distinct species. To discriminate these species, we compared metabolic profiles of F. gummosa and F. galbaniflua samples. METHODS 1 H-NMR-based metabolomics analysis was used for classification of F. gummosa and F. galbaniflua samples collected from northeast Iran. The acquired data were analyzed using hierarchical cluster analysis (HCA), partial least squares discriminant analysis (PLS-DA) and orthogonal projection to latent structures discriminant analysis (OPLS-DA). RESULTS The result showed a clear separation between the two species that may be related to the quantity and diversity of their metabolites. Ferula gummosa had higher mogoltacin levels, while F. galbaniflua had higher feselol levels. Ligupersin A and conferdione were significantly detected in F. gummosa, whereas sterol compounds were significantly detected in F. galbaniflua. CONCLUSION Our findings indicate that clear metabolomics discrimination of F. gummosa and F. galbaniflua makes their chemotaxonomic classification possible.
