The Experts below are selected from a list of 6831 Experts worldwide ranked by ideXlab platform
Tao Suo - One of the best experts on this subject based on the ideXlab platform.
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degradable magnesium implants inhibit Gallbladder Cancer
Acta Biomaterialia, 2021Co-Authors: Hongzhou Peng, Kun Fan, Rui Zan, Zijun Gong, Wentao Sun, Yu Sun, Wenhui Wang, Haomiao Jiang, Jie Lou, Tao SuoAbstract:Gallbladder Cancer can be difficult to detect in its early stages and is prone to metastasize, causing bile duct obstruction, which is usually treated by stent implantation in clinic. However, the commonly used biliary stents are non-degradable, which not only prone to secondary blockage, but also need to be removed by secondary surgery. Biodegradable magnesium (Mg) is expected to one of the promising candidates for degradable biliary stents due to its excellent physicochemical property and biocompatibility. In this work, we studied the influence of high-purity Mg wires on Gallbladder Cancer through in vitro and in vivo experiments and revealed that the degradation products of Mg could significantly inhibit the growth of Gallbladder Cancer cells and promote their apoptosis. Our findings indicate that Mg biliary stent possesses the function of draining bile and treating Gallbladder Cancer, suggesting that Mg has good application prospects in biliary surgery. STATEMENT OF SIGNIFICANCE: Current research and development of biomedical magnesium are mainly concentrated in the cardiovascular and orthopedics field. Degradable magnesium bile duct stents have great application prospects in the treatment of bile duct blockage caused by bile duct-related Cancers. At present, the effect of magnesium implants on Gallbladder Cancer is not clear. Our work verified the effectiveness of magnesium wire implants in inhibiting Gallbladder Cancer through in vivo and in vitro experiments, and studied the effect of magnesium degradation products on Gallbladder Cancer cells from the perspective of cell proliferation, apoptosis and cycle. This study provided new understanding for the application of magnesium in biliary surgery.
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degradable magnesium implants inhibit Gallbladder Cancer
Social Science Research Network, 2021Co-Authors: Hongzhou Peng, Kun Fan, Rui Zan, Zijun Gong, Wentao Sun, Yu Sun, Wenhui Wang, Haomiao Jiang, Jie Lou, Tao SuoAbstract:Gallbladder Cancer can be difficult to detect in its early stages and is prone to metastasize, causing bile duct obstruction, which is usually treated by stent implantation in clinic. However, the commonly used biliary stents are non-degradable, which not only prone to secondary blockage, but also need to be removed by secondary surgery. Biodegradable magnesium (Mg) are expected to one of the promising candidates for degradable biliary stents due to its excellent physicochemical property and biocompatibility. In this work, we study the influence of high-purity Mg wires on Gallbladder Cancer through in vitro and in vivo experiments, and reveal that the degradation products of Mg could significantly inhibit the growth of Gallbladder Cancer cells and promote their apoptosis. Our findings indicate that Mg biliary stent possesses the function of draining bile and treating Gallbladder Cancer, suggesting that Mg has good application prospects in biliary surgery.
Fei Zhang - One of the best experts on this subject based on the ideXlab platform.
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Tea polyphenols induce S phase arrest and apoptosis in Gallbladder Cancer cells
Associação Brasileira de Divulgação Científica, 2018Co-Authors: Jiaqi Wang, Yijian Zhang, Fei Zhang, Yixuan Pan, Yingbin LiuAbstract:Gallbladder Cancer (GBC) is the most common malignancy in the biliary tract. Without effective treatment, its prognosis is notoriously poor. Tea polyphenols (TPs) have many pharmacological and health benefits, including antioxidant, anti-inflammatory, anti-tumor, anti-thrombotic, antibacterial, and vasodilatory properties. However, the anti-Cancer effect of TPs in human Gallbladder Cancer has not yet been determined. Cell viability and colony formation assay were used to investigate the cell growth. Cell cycle and apoptosis were evaluated by flow cytometry analysis. Western blot assay was used to detect the expression of proteins related to cell cycle and apoptosis. Human tumor xenografts were used to examine the effect of TPs on Gallbladder Cancer cells in vivo. TPs significantly inhibited cell growth of Gallbladder Cancer cell lines in a dose- and time-dependent manner. Cell cycle progression in GBC cells was blocked at the S phase by TPs. TPs also induced mitochondrial-related apoptosis in GBC cells by upregulating Bax, cleaved caspase-3, and cleaved PARP expressions and downregulating Bcl-2, cyclin A, and Cdk2 expressions. The effects of TPs on GBC were further proven in vivo in a mouse xenograft model. Our study is the first to report that TPs inhibit GBC cell growth and these compounds may have potential as novel therapeutic agents for treating Gallbladder Cancer
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dihydroartemisinin inhibits tctp dependent metastasis in Gallbladder Cancer
Journal of Experimental & Clinical Cancer Research, 2017Co-Authors: Fei Zhang, Zihang Xu, Haibin Liang, Huaifeng Li, Yuanyuan Ye, Shanshan Xiang, Yunping Hu, Yijian Zhang, Lin Jiang, Zheng WangAbstract:Abstract Background Patients with metastatic or relapsed Gallbladder Cancer generally have a poor prognosis. Therefore, targeting metastasis is one arm of therapeutic strategies to treat Gallbladder Cancer. Methods Levels of translationally controlled tumor protein (TCTP) were measured in samples of Gallbladder Cancer by immunohistochemical staining. Wound healing, migration and invasion assays were used to investigate the motility of cells. Western blot assay was used to investigate the levels of TCTP and other proteins. Liver metastasis models and lung metastasis models were established to investigate the inhibitory effect of Dihydroartemisinin on Gallbladder Cancer metastasis. Results TCTP is aberrantly expressed in Gallbladder Cancer patients and associated with metastasis and a poor prognosis. Depleting TCTP significantly inhibited Gallbladder Cancer cell migration and invasion. We found that Dihydroartemisinin as a potent inhibitor of TCTP inhibited TCTP-dependent cell migration and invasion by reducing cell division control protein 42 homolog (Cdc42) activation. In addition, in mice with xenografted tumors, treatment with Dihydroartemisinin decreased Gallbladder Cancer cell metastases and improved survival. Conclusions These findings provide new insights into the therapeutic activity of Dihydroartemisinin as a treatment for Gallbladder Cancer metastasis.
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dihydroartemisinin inhibits tctp dependent metastasis in Gallbladder Cancer
Journal of Experimental & Clinical Cancer Research, 2017Co-Authors: Fei Zhang, Haibin Liang, Shanshan Xiang, Yijian Zhang, Zheng Wang, Lin Jiang, Wei Gong, Xuefeng Wang, Yong Zhang, Yingbin LiuAbstract:Patients with metastatic or relapsed Gallbladder Cancer generally have a poor prognosis. Therefore, targeting metastasis is one arm of therapeutic strategies to treat Gallbladder Cancer. Levels of translationally controlled tumor protein (TCTP) were measured in samples of Gallbladder Cancer by immunohistochemical staining. Wound healing, migration and invasion assays were used to investigate the motility of cells. Western blot assay was used to investigate the levels of TCTP and other proteins. Liver metastasis models and lung metastasis models were established to investigate the inhibitory effect of Dihydroartemisinin on Gallbladder Cancer metastasis. TCTP is aberrantly expressed in Gallbladder Cancer patients and associated with metastasis and a poor prognosis. Depleting TCTP significantly inhibited Gallbladder Cancer cell migration and invasion. We found that Dihydroartemisinin as a potent inhibitor of TCTP inhibited TCTP-dependent cell migration and invasion by reducing cell division control protein 42 homolog (Cdc42) activation. In addition, in mice with xenografted tumors, treatment with Dihydroartemisinin decreased Gallbladder Cancer cell metastases and improved survival. These findings provide new insights into the therapeutic activity of Dihydroartemisinin as a treatment for Gallbladder Cancer metastasis.
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oleanolic acid induces mitochondrial dependent apoptosis and g0 g1 phase arrest in Gallbladder Cancer cells
Drug Design Development and Therapy, 2015Co-Authors: Xuan Wang, Shanshan Xiang, Fei Zhang, Lin Jiang, Hao Weng, Runfa Bao, Yijun Shu, Yang Cao, Qian Dong, Yingbin LiuAbstract:Oleanolic acid (OA), a naturally occurring triterpenoid, exhibits potential antitumor activity in many tumor cell lines. Gallbladder carcinoma is the most common malignancy of the biliary tract, and is a highly aggressive tumor with an extremely poor prognosis. Unfortunately, the effects of OA on Gallbladder carcinoma are unknown. In this study, we investigated the effects of OA on Gallbladder Cancer cells and the underlying mechanism. The results showed that OA inhibits proliferation of Gallbladder Cancer cells in a dose-dependent and time-dependent manner on MTT and colony formation assay. A flow cytometry assay revealed apoptosis and G0/G1 phase arrest in GBC-SD and NOZ cells. Western blot analysis and a mitochondrial membrane potential assay demonstrated that OA functions through the mitochondrial apoptosis pathway. Moreover, this drug inhibited tumor growth in nude mice carrying subcutaneous NOZ tumor xenografts. These data suggest that OA inhibits proliferation of Gallbladder Cancer cells by regulating apoptosis and the cell cycle process. Thus, OA may be a promising drug for adjuvant chemotherapy in Gallbladder carcinoma.
Yingbin Liu - One of the best experts on this subject based on the ideXlab platform.
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Tea polyphenols induce S phase arrest and apoptosis in Gallbladder Cancer cells
Associação Brasileira de Divulgação Científica, 2018Co-Authors: Jiaqi Wang, Yijian Zhang, Fei Zhang, Yixuan Pan, Yingbin LiuAbstract:Gallbladder Cancer (GBC) is the most common malignancy in the biliary tract. Without effective treatment, its prognosis is notoriously poor. Tea polyphenols (TPs) have many pharmacological and health benefits, including antioxidant, anti-inflammatory, anti-tumor, anti-thrombotic, antibacterial, and vasodilatory properties. However, the anti-Cancer effect of TPs in human Gallbladder Cancer has not yet been determined. Cell viability and colony formation assay were used to investigate the cell growth. Cell cycle and apoptosis were evaluated by flow cytometry analysis. Western blot assay was used to detect the expression of proteins related to cell cycle and apoptosis. Human tumor xenografts were used to examine the effect of TPs on Gallbladder Cancer cells in vivo. TPs significantly inhibited cell growth of Gallbladder Cancer cell lines in a dose- and time-dependent manner. Cell cycle progression in GBC cells was blocked at the S phase by TPs. TPs also induced mitochondrial-related apoptosis in GBC cells by upregulating Bax, cleaved caspase-3, and cleaved PARP expressions and downregulating Bcl-2, cyclin A, and Cdk2 expressions. The effects of TPs on GBC were further proven in vivo in a mouse xenograft model. Our study is the first to report that TPs inhibit GBC cell growth and these compounds may have potential as novel therapeutic agents for treating Gallbladder Cancer
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dihydroartemisinin inhibits tctp dependent metastasis in Gallbladder Cancer
Journal of Experimental & Clinical Cancer Research, 2017Co-Authors: Fei Zhang, Haibin Liang, Shanshan Xiang, Yijian Zhang, Zheng Wang, Lin Jiang, Wei Gong, Xuefeng Wang, Yong Zhang, Yingbin LiuAbstract:Patients with metastatic or relapsed Gallbladder Cancer generally have a poor prognosis. Therefore, targeting metastasis is one arm of therapeutic strategies to treat Gallbladder Cancer. Levels of translationally controlled tumor protein (TCTP) were measured in samples of Gallbladder Cancer by immunohistochemical staining. Wound healing, migration and invasion assays were used to investigate the motility of cells. Western blot assay was used to investigate the levels of TCTP and other proteins. Liver metastasis models and lung metastasis models were established to investigate the inhibitory effect of Dihydroartemisinin on Gallbladder Cancer metastasis. TCTP is aberrantly expressed in Gallbladder Cancer patients and associated with metastasis and a poor prognosis. Depleting TCTP significantly inhibited Gallbladder Cancer cell migration and invasion. We found that Dihydroartemisinin as a potent inhibitor of TCTP inhibited TCTP-dependent cell migration and invasion by reducing cell division control protein 42 homolog (Cdc42) activation. In addition, in mice with xenografted tumors, treatment with Dihydroartemisinin decreased Gallbladder Cancer cell metastases and improved survival. These findings provide new insights into the therapeutic activity of Dihydroartemisinin as a treatment for Gallbladder Cancer metastasis.
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oleanolic acid induces mitochondrial dependent apoptosis and g0 g1 phase arrest in Gallbladder Cancer cells
Drug Design Development and Therapy, 2015Co-Authors: Xuan Wang, Shanshan Xiang, Fei Zhang, Lin Jiang, Hao Weng, Runfa Bao, Yijun Shu, Yang Cao, Qian Dong, Yingbin LiuAbstract:Oleanolic acid (OA), a naturally occurring triterpenoid, exhibits potential antitumor activity in many tumor cell lines. Gallbladder carcinoma is the most common malignancy of the biliary tract, and is a highly aggressive tumor with an extremely poor prognosis. Unfortunately, the effects of OA on Gallbladder carcinoma are unknown. In this study, we investigated the effects of OA on Gallbladder Cancer cells and the underlying mechanism. The results showed that OA inhibits proliferation of Gallbladder Cancer cells in a dose-dependent and time-dependent manner on MTT and colony formation assay. A flow cytometry assay revealed apoptosis and G0/G1 phase arrest in GBC-SD and NOZ cells. Western blot analysis and a mitochondrial membrane potential assay demonstrated that OA functions through the mitochondrial apoptosis pathway. Moreover, this drug inhibited tumor growth in nude mice carrying subcutaneous NOZ tumor xenografts. These data suggest that OA inhibits proliferation of Gallbladder Cancer cells by regulating apoptosis and the cell cycle process. Thus, OA may be a promising drug for adjuvant chemotherapy in Gallbladder carcinoma.
Zheng Wang - One of the best experts on this subject based on the ideXlab platform.
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dihydroartemisinin inhibits tctp dependent metastasis in Gallbladder Cancer
Journal of Experimental & Clinical Cancer Research, 2017Co-Authors: Fei Zhang, Zihang Xu, Haibin Liang, Huaifeng Li, Yuanyuan Ye, Shanshan Xiang, Yunping Hu, Yijian Zhang, Lin Jiang, Zheng WangAbstract:Abstract Background Patients with metastatic or relapsed Gallbladder Cancer generally have a poor prognosis. Therefore, targeting metastasis is one arm of therapeutic strategies to treat Gallbladder Cancer. Methods Levels of translationally controlled tumor protein (TCTP) were measured in samples of Gallbladder Cancer by immunohistochemical staining. Wound healing, migration and invasion assays were used to investigate the motility of cells. Western blot assay was used to investigate the levels of TCTP and other proteins. Liver metastasis models and lung metastasis models were established to investigate the inhibitory effect of Dihydroartemisinin on Gallbladder Cancer metastasis. Results TCTP is aberrantly expressed in Gallbladder Cancer patients and associated with metastasis and a poor prognosis. Depleting TCTP significantly inhibited Gallbladder Cancer cell migration and invasion. We found that Dihydroartemisinin as a potent inhibitor of TCTP inhibited TCTP-dependent cell migration and invasion by reducing cell division control protein 42 homolog (Cdc42) activation. In addition, in mice with xenografted tumors, treatment with Dihydroartemisinin decreased Gallbladder Cancer cell metastases and improved survival. Conclusions These findings provide new insights into the therapeutic activity of Dihydroartemisinin as a treatment for Gallbladder Cancer metastasis.
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dihydroartemisinin inhibits tctp dependent metastasis in Gallbladder Cancer
Journal of Experimental & Clinical Cancer Research, 2017Co-Authors: Fei Zhang, Haibin Liang, Shanshan Xiang, Yijian Zhang, Zheng Wang, Lin Jiang, Wei Gong, Xuefeng Wang, Yong Zhang, Yingbin LiuAbstract:Patients with metastatic or relapsed Gallbladder Cancer generally have a poor prognosis. Therefore, targeting metastasis is one arm of therapeutic strategies to treat Gallbladder Cancer. Levels of translationally controlled tumor protein (TCTP) were measured in samples of Gallbladder Cancer by immunohistochemical staining. Wound healing, migration and invasion assays were used to investigate the motility of cells. Western blot assay was used to investigate the levels of TCTP and other proteins. Liver metastasis models and lung metastasis models were established to investigate the inhibitory effect of Dihydroartemisinin on Gallbladder Cancer metastasis. TCTP is aberrantly expressed in Gallbladder Cancer patients and associated with metastasis and a poor prognosis. Depleting TCTP significantly inhibited Gallbladder Cancer cell migration and invasion. We found that Dihydroartemisinin as a potent inhibitor of TCTP inhibited TCTP-dependent cell migration and invasion by reducing cell division control protein 42 homolog (Cdc42) activation. In addition, in mice with xenografted tumors, treatment with Dihydroartemisinin decreased Gallbladder Cancer cell metastases and improved survival. These findings provide new insights into the therapeutic activity of Dihydroartemisinin as a treatment for Gallbladder Cancer metastasis.
Hongzhou Peng - One of the best experts on this subject based on the ideXlab platform.
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degradable magnesium implants inhibit Gallbladder Cancer
Acta Biomaterialia, 2021Co-Authors: Hongzhou Peng, Kun Fan, Rui Zan, Zijun Gong, Wentao Sun, Yu Sun, Wenhui Wang, Haomiao Jiang, Jie Lou, Tao SuoAbstract:Gallbladder Cancer can be difficult to detect in its early stages and is prone to metastasize, causing bile duct obstruction, which is usually treated by stent implantation in clinic. However, the commonly used biliary stents are non-degradable, which not only prone to secondary blockage, but also need to be removed by secondary surgery. Biodegradable magnesium (Mg) is expected to one of the promising candidates for degradable biliary stents due to its excellent physicochemical property and biocompatibility. In this work, we studied the influence of high-purity Mg wires on Gallbladder Cancer through in vitro and in vivo experiments and revealed that the degradation products of Mg could significantly inhibit the growth of Gallbladder Cancer cells and promote their apoptosis. Our findings indicate that Mg biliary stent possesses the function of draining bile and treating Gallbladder Cancer, suggesting that Mg has good application prospects in biliary surgery. STATEMENT OF SIGNIFICANCE: Current research and development of biomedical magnesium are mainly concentrated in the cardiovascular and orthopedics field. Degradable magnesium bile duct stents have great application prospects in the treatment of bile duct blockage caused by bile duct-related Cancers. At present, the effect of magnesium implants on Gallbladder Cancer is not clear. Our work verified the effectiveness of magnesium wire implants in inhibiting Gallbladder Cancer through in vivo and in vitro experiments, and studied the effect of magnesium degradation products on Gallbladder Cancer cells from the perspective of cell proliferation, apoptosis and cycle. This study provided new understanding for the application of magnesium in biliary surgery.
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degradable magnesium implants inhibit Gallbladder Cancer
Social Science Research Network, 2021Co-Authors: Hongzhou Peng, Kun Fan, Rui Zan, Zijun Gong, Wentao Sun, Yu Sun, Wenhui Wang, Haomiao Jiang, Jie Lou, Tao SuoAbstract:Gallbladder Cancer can be difficult to detect in its early stages and is prone to metastasize, causing bile duct obstruction, which is usually treated by stent implantation in clinic. However, the commonly used biliary stents are non-degradable, which not only prone to secondary blockage, but also need to be removed by secondary surgery. Biodegradable magnesium (Mg) are expected to one of the promising candidates for degradable biliary stents due to its excellent physicochemical property and biocompatibility. In this work, we study the influence of high-purity Mg wires on Gallbladder Cancer through in vitro and in vivo experiments, and reveal that the degradation products of Mg could significantly inhibit the growth of Gallbladder Cancer cells and promote their apoptosis. Our findings indicate that Mg biliary stent possesses the function of draining bile and treating Gallbladder Cancer, suggesting that Mg has good application prospects in biliary surgery.
