The Experts below are selected from a list of 273 Experts worldwide ranked by ideXlab platform
Giovanni Vacca - One of the best experts on this subject based on the ideXlab platform.
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receptors and nitric oxide in anesthetized pigs cck and function through autonomic nervous system intracoronary Gastrin 17 increases cardiac perfusion
2015Co-Authors: Giovanni Vacca, Elena Grossini, Claudio Molinari, Philippe Primo Caimmi, Francesca Uberti, L Sigaudo, M Biella, David MaryAbstract:Calcium handling in porcine coronary endothelial cells by Gastrin-17 (PDF) (Full Text) (Abstract) -adrenergic-related pathway and nitric oxide β function through a In anesthetized pigs human chorionic gonadotropin increases myocardial perfusion and (PDF) (Full Text) (Abstract)
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calcium handling in porcine coronary endothelial cells by Gastrin 17
Journal of Molecular Endocrinology, 2013Co-Authors: Elena Grossini, Claudio Molinari, Lorenzo Sigaudo, Monica Biella, David Mary, Giovanni VaccaAbstract:In porcine coronary artery endothelial cells (PCAEC), Gastrin-17 has recently been found to increase nitric oxide (NO) production by the endothelial NO synthase (eNOS) isoform through cholecystokinin 1/2 (CCK1/2) receptors and the involvement of protein kinase A (PKA), PKC and the b2-adrenoreceptor-related pathway. As eNOS is the Ca 2C -dependent isoform of the enzyme, we aimed to examine the effects of Gastrin-17 on Ca 2C movements. Thus, experiments were performed in Fura-2-acetoxymethyl-ester-loaded PCAEC, where changes of cytosolic Ca 2C ([Ca 2C ]c) caused by Gastrin-17 were analysed and compared with those of CCK receptors and b2-adrenoreceptors agonists/antagonists. In addition, some experiments were performed by stimulating cells with Gastrin-17 in the presence or absence of cAMP/PKA activator/inhibitor and of phospholipase C (PLC) and Ca 2C –calmodulin dependent protein kinase II (CaMKII) blockers. The results have shown that Gastrin-17 can promote a transient increase in [Ca 2C ]c mainly originating from an intracellular pool sensitive to thapsigargin and from the extracellular space. In addition, the response of cells to Gastrin-17 was increased by the adenylyl cyclase activator and the b2-adrenoreceptor agonists and affected mainly by the CCK2 receptor agonists/antagonists. Moreover, the effects of Gastrin-17 were prevented by b2-adrenoreceptors and CaMKII blockers and the adenylyl cyclase/PKA and PLC inhibitors. Finally, in PCAEC cultured in Na C -free medium or loaded with the plasma membrane Ca 2C pump inhibitor, the Gastrin-17-evoked Ca 2C transient was long lasting. In conclusion, this study shows that Gastrin-17 affected intracellular Ca 2C homeostasis in PCAEC by both promoting a discharge of an intracellular pool and by interfering with the operation of store-dependent channels through mainly CCK2 receptors and PKA/PLC- and CaMKII-related signalling downstream of b2-adrenoreceptor stimulation.
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cck receptors related signaling involved in nitric oxide production caused by Gastrin 17 in porcine coronary endothelial cells
Molecular and Cellular Endocrinology, 2012Co-Authors: Elena Grossini, Claudio Molinari, David Mary, Philippe Primo Caimmi, Francesca Uberti, Giovanni VaccaAbstract:Abstract In anesthetized pigs Gastrin-17 increased coronary blood flow through CCK1/CCK2 receptors and β2-adrenoceptors-related nitric oxide (NO) release. Since the intracellular pathway has not been investigated the purpose of this study was to examine in coronary endothelial cells the CCK1/CCK2 receptors-related signaling involved in the effects of Gastrin-17 on NO release. Gastrin-17 caused a concentration-dependent increase of NO production (17.3–62.6%; p
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intracoronary Gastrin 17 increases cardiac perfusion and function through autonomic nervous system cck receptors and nitric oxide in anesthetized pigs
Journal of Applied Physiology, 2011Co-Authors: Elena Grossini, Claudio Molinari, David Mary, Philippe Primo Caimmi, Francesca Uberti, Giovanni VaccaAbstract:The release of gastrointestinal hormones has been reported to modulate reflex cardiovascular responses caused by gastric distension, although the role played by Gastrin 17 is as yet unknown. The pr...
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Intracoronary Gastrin 17 increases cardiac perfusion and function through autonomic nervous system, CCK receptors, and nitric oxide in anesthetized pigs
Journal of Applied Physiology, 2010Co-Authors: Elena Grossini, Claudio Molinari, David Mary, Philippe Primo Caimmi, Francesca Uberti, Giovanni VaccaAbstract:The release of gastrointestinal hormones has been reported to modulate reflex cardiovascular responses caused by gastric distension, although the role played by Gastrin 17 is as yet unknown. The present study was therefore planned to determine the primary in vivo effect of Gastrin 17 on coronary blood flow and cardiac function and the involvement of autonomic nervous system, CCK1/2 receptors, and nitric oxide (NO). In 40 anesthetized pigs, Gastrin 17 was infused into the left anterior descending coronary artery at constant heart rate and arterial blood pressure. In 35 of the 40 pigs, the mechanisms of the observed hemodynamic responses were analyzed by repeating Gastrin 17 infusion after autonomic nervous system and NO blockade, and after specific CCK receptors agonists/antagonists administration. Intracoronary Gastrin 17 administration caused dose-related increases of both coronary blood flow and cardiac function. The intracoronary co-administration of CCK33/pentaGastrin and Gastrin 17 potentiated the co...
Yuan Yuan - One of the best experts on this subject based on the ideXlab platform.
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a comprehensive evaluation of fasting serum Gastrin 17 as a predictor of diseased stomach in chinese population
Scandinavian Journal of Gastroenterology, 2014Co-Authors: Huakang Tu, Yue Hua Gong, Qian Xu, Jingjing Jing, Nannan Dong, Yuan YuanAbstract:AbstractBackground and aim. Fasting serum Gastrin-17 (FsG17) is considered as a noninvasive biomarker reflecting the structure and functional status of gastric mucosa, but its clinical utility remains unclear. This study aimed to evaluate FsG17 comprehensively: establish the ranges and cut-off points of FsG17 levels in different gastric diseases, identify their influencing factors, and investigate the accuracy of FsG17 for identifying diseased stomach. Methods. The study included 4064 participants from Northern China between 2008 and 2013. FsG17 and serum Helicobacter pylori IgG antibody levels were measured by enzyme-linked immunosorbent assay. Diagnostic accuracy was assessed by receiver operator characteristic curves. Multivariate logistic regression analysis was performed to determine the best predictors of gastric histopathological conditions. Results. Median FsG17 levels in healthy, non-atrophic, atrophic, and cancerous stomachs were 1.8, 4.0, 3.8, and 6.1 pmol/l, respectively. Age, smoking status, ...
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abstract c13 population based evaluation of serum pepsinogen anti h pylori igg antibody and Gastrin 17 tests for gastric cancer screening
Cancer Prevention Research, 2013Co-Authors: Huakang Tu, Yue Hua Gong, Qian Xu, Qi Long, Roberd M Bostick, Yuan YuanAbstract:Objective: This study evaluated the role of multiple serological tests (including serum pepsinogen I (PGI), PGII, PGI/II ratio, anti-H. pylori IgG antibody, and Gastrin-17 tests) individually and in combination in gastric cancer (GC) screening. Design: Data were from the Zhuanghe Gastric Cancer Study, a 15-year, population-based endoscopic, gastric diseases screening program in northern China. From 1997 to 2011, esophagogastroduodenoscopies with gastric mucosal biopsies were conducted on 10,693 participants. All serum biomarkers were measured using ELISA, and gastric biopsies were evaluated using standardized criteria. Logistic regression was used to calculate odds ratios (OR) with 95 percent confidence intervals (95% CI) and produce receiver operator characteristic (ROC) curves with corresponding c statistics. In addition, results were replicated in an independent hospital-based population. Results: PGI/II ratio monotonically decreased as gastric mucosa progresses from normal mucosa to precancerous conditions, and to GC, and a low PGI/II ratio (≥ 6.6) was associated with an increased risk of prevalent GC (OR = 2.34, 95% CI: 1.62, 3.39). PGI/II ratio individually or in combination with PGI, PGII, anti-H. pylori IgG antibody and Gastrin-17 provided the same c statistic of 0.62 for screening for GC. PGI, PGII, anti-H. pylori IgG antibody or Gastrin-17 tests individually provided c statistics less than or equal to 0.55. For identifying abnormal gastric conditions including GC, the five serological tests in combination provided adequate discriminatory performance (c = 0.77), and yielded a sensitivity of 84.0% and specificity of 50.0%. The results from the independent hospital-based population were similar. Conclusion: Our results suggest that serum PGI, PGII, PGI/II ratio, anti-H. pylori IgG and Gastrin-17 tests can be used as prescreening tests for patients at high risk for GC. Citation Format: Huakang Tu, Liping Sun, Yuehua Gong, Qian Xu, Qi Long, Roberd Bostick, Yuan Yuan. Population-based evaluation of serum pepsinogen, anti-H. pylori IgG antibody and Gastrin-17 tests for gastric cancer screening. [abstract]. In: Proceedings of the Twelfth Annual AACR International Conference on Frontiers in Cancer Prevention Research; 2013 Oct 27-30; National Harbor, MD. Philadelphia (PA): AACR; Can Prev Res 2013;6(11 Suppl): Abstract nr C13.
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factors affecting the serum Gastrin 17 level an evidence based analysis of 3906 serum samples among chinese
Journal of Digestive Diseases, 2007Co-Authors: Zhong Zhang, Yue Hua Gong, Xu Guang Wang, Meng Zhang, Yuan YuanAbstract:OBJECTIVE: To investigate the influence of gender, age, site of lesion, disease type and Helicobacter pylori (H. pylori) infection on the human serum Gastrin-17 level and to study the diagnostic value of serum Gastrin-17 in gastric precancerous lesions and gastric cancer. METHODS: Serum Gastrin-17 and serum H. pylori IgG antibody were detected by the ELISA method. The different gastric disease groups were confirmed by endoscopy and histopathology. RESULTS: Among the 3906 serum samples according to the gender, age, site of lesion and the data of different gastric disease groups, the serum Gastrin-17 level was markedly higher in people ≥60 years old than that in younger age groups. The serum Gastrin-17 level increased progressively in the following order: healthy control group, nonatrophic gastritis group, gastric ulcer group, and the serum Gastrin-17 level was higher in the atrophic gastritis with dysplasia group than that without it, the lowest level being in the gastric cancer group. Among the 2946 serum samples matched with the site of the lesion, the serum Gastrin-17 level was higher in those with antral diseases than in those with gastric corpus diseases. Among the 3805 serum samples matched with the H. pylori infection data, the serum Gastrin-17 level was higher in the H. pylori-positive group than in the H. pylori-negative group. CONCLUSIONS: In people over 60 years of age, the serum Gastrin-17 level tends to increase. In subjects with precancerous gastric lesions, it may increase significantly with the progression of gastric disease, and ultimately decrease in gastric cancer. Serum Gastrin-17 is a good biomarker to differentiate benign from malignant gastric diseases. The site of the gastric lesions is an important factor affecting the serum Gastrin-17 level, whereas H. pylori infection is usually associated with its increment.
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Factors affecting the serum Gastrin 17 level: an evidence‐based analysis of 3906 serum samples among Chinese
Journal of Digestive Diseases, 2007Co-Authors: Zhong Zhang, Yue Hua Gong, Xu Guang Wang, Meng Zhang, Yuan YuanAbstract:OBJECTIVE: To investigate the influence of gender, age, site of lesion, disease type and Helicobacter pylori (H. pylori) infection on the human serum Gastrin-17 level and to study the diagnostic value of serum Gastrin-17 in gastric precancerous lesions and gastric cancer. METHODS: Serum Gastrin-17 and serum H. pylori IgG antibody were detected by the ELISA method. The different gastric disease groups were confirmed by endoscopy and histopathology. RESULTS: Among the 3906 serum samples according to the gender, age, site of lesion and the data of different gastric disease groups, the serum Gastrin-17 level was markedly higher in people ≥60 years old than that in younger age groups. The serum Gastrin-17 level increased progressively in the following order: healthy control group, nonatrophic gastritis group, gastric ulcer group, and the serum Gastrin-17 level was higher in the atrophic gastritis with dysplasia group than that without it, the lowest level being in the gastric cancer group. Among the 2946 serum samples matched with the site of the lesion, the serum Gastrin-17 level was higher in those with antral diseases than in those with gastric corpus diseases. Among the 3805 serum samples matched with the H. pylori infection data, the serum Gastrin-17 level was higher in the H. pylori-positive group than in the H. pylori-negative group. CONCLUSIONS: In people over 60 years of age, the serum Gastrin-17 level tends to increase. In subjects with precancerous gastric lesions, it may increase significantly with the progression of gastric disease, and ultimately decrease in gastric cancer. Serum Gastrin-17 is a good biomarker to differentiate benign from malignant gastric diseases. The site of the gastric lesions is an important factor affecting the serum Gastrin-17 level, whereas H. pylori infection is usually associated with its increment.
Elena Grossini - One of the best experts on this subject based on the ideXlab platform.
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receptors and nitric oxide in anesthetized pigs cck and function through autonomic nervous system intracoronary Gastrin 17 increases cardiac perfusion
2015Co-Authors: Giovanni Vacca, Elena Grossini, Claudio Molinari, Philippe Primo Caimmi, Francesca Uberti, L Sigaudo, M Biella, David MaryAbstract:Calcium handling in porcine coronary endothelial cells by Gastrin-17 (PDF) (Full Text) (Abstract) -adrenergic-related pathway and nitric oxide β function through a In anesthetized pigs human chorionic gonadotropin increases myocardial perfusion and (PDF) (Full Text) (Abstract)
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calcium handling in porcine coronary endothelial cells by Gastrin 17
Journal of Molecular Endocrinology, 2013Co-Authors: Elena Grossini, Claudio Molinari, Lorenzo Sigaudo, Monica Biella, David Mary, Giovanni VaccaAbstract:In porcine coronary artery endothelial cells (PCAEC), Gastrin-17 has recently been found to increase nitric oxide (NO) production by the endothelial NO synthase (eNOS) isoform through cholecystokinin 1/2 (CCK1/2) receptors and the involvement of protein kinase A (PKA), PKC and the b2-adrenoreceptor-related pathway. As eNOS is the Ca 2C -dependent isoform of the enzyme, we aimed to examine the effects of Gastrin-17 on Ca 2C movements. Thus, experiments were performed in Fura-2-acetoxymethyl-ester-loaded PCAEC, where changes of cytosolic Ca 2C ([Ca 2C ]c) caused by Gastrin-17 were analysed and compared with those of CCK receptors and b2-adrenoreceptors agonists/antagonists. In addition, some experiments were performed by stimulating cells with Gastrin-17 in the presence or absence of cAMP/PKA activator/inhibitor and of phospholipase C (PLC) and Ca 2C –calmodulin dependent protein kinase II (CaMKII) blockers. The results have shown that Gastrin-17 can promote a transient increase in [Ca 2C ]c mainly originating from an intracellular pool sensitive to thapsigargin and from the extracellular space. In addition, the response of cells to Gastrin-17 was increased by the adenylyl cyclase activator and the b2-adrenoreceptor agonists and affected mainly by the CCK2 receptor agonists/antagonists. Moreover, the effects of Gastrin-17 were prevented by b2-adrenoreceptors and CaMKII blockers and the adenylyl cyclase/PKA and PLC inhibitors. Finally, in PCAEC cultured in Na C -free medium or loaded with the plasma membrane Ca 2C pump inhibitor, the Gastrin-17-evoked Ca 2C transient was long lasting. In conclusion, this study shows that Gastrin-17 affected intracellular Ca 2C homeostasis in PCAEC by both promoting a discharge of an intracellular pool and by interfering with the operation of store-dependent channels through mainly CCK2 receptors and PKA/PLC- and CaMKII-related signalling downstream of b2-adrenoreceptor stimulation.
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cck receptors related signaling involved in nitric oxide production caused by Gastrin 17 in porcine coronary endothelial cells
Molecular and Cellular Endocrinology, 2012Co-Authors: Elena Grossini, Claudio Molinari, David Mary, Philippe Primo Caimmi, Francesca Uberti, Giovanni VaccaAbstract:Abstract In anesthetized pigs Gastrin-17 increased coronary blood flow through CCK1/CCK2 receptors and β2-adrenoceptors-related nitric oxide (NO) release. Since the intracellular pathway has not been investigated the purpose of this study was to examine in coronary endothelial cells the CCK1/CCK2 receptors-related signaling involved in the effects of Gastrin-17 on NO release. Gastrin-17 caused a concentration-dependent increase of NO production (17.3–62.6%; p
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intracoronary Gastrin 17 increases cardiac perfusion and function through autonomic nervous system cck receptors and nitric oxide in anesthetized pigs
Journal of Applied Physiology, 2011Co-Authors: Elena Grossini, Claudio Molinari, David Mary, Philippe Primo Caimmi, Francesca Uberti, Giovanni VaccaAbstract:The release of gastrointestinal hormones has been reported to modulate reflex cardiovascular responses caused by gastric distension, although the role played by Gastrin 17 is as yet unknown. The pr...
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Intracoronary Gastrin 17 increases cardiac perfusion and function through autonomic nervous system, CCK receptors, and nitric oxide in anesthetized pigs
Journal of Applied Physiology, 2010Co-Authors: Elena Grossini, Claudio Molinari, David Mary, Philippe Primo Caimmi, Francesca Uberti, Giovanni VaccaAbstract:The release of gastrointestinal hormones has been reported to modulate reflex cardiovascular responses caused by gastric distension, although the role played by Gastrin 17 is as yet unknown. The present study was therefore planned to determine the primary in vivo effect of Gastrin 17 on coronary blood flow and cardiac function and the involvement of autonomic nervous system, CCK1/2 receptors, and nitric oxide (NO). In 40 anesthetized pigs, Gastrin 17 was infused into the left anterior descending coronary artery at constant heart rate and arterial blood pressure. In 35 of the 40 pigs, the mechanisms of the observed hemodynamic responses were analyzed by repeating Gastrin 17 infusion after autonomic nervous system and NO blockade, and after specific CCK receptors agonists/antagonists administration. Intracoronary Gastrin 17 administration caused dose-related increases of both coronary blood flow and cardiac function. The intracoronary co-administration of CCK33/pentaGastrin and Gastrin 17 potentiated the co...
Claudio Molinari - One of the best experts on this subject based on the ideXlab platform.
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receptors and nitric oxide in anesthetized pigs cck and function through autonomic nervous system intracoronary Gastrin 17 increases cardiac perfusion
2015Co-Authors: Giovanni Vacca, Elena Grossini, Claudio Molinari, Philippe Primo Caimmi, Francesca Uberti, L Sigaudo, M Biella, David MaryAbstract:Calcium handling in porcine coronary endothelial cells by Gastrin-17 (PDF) (Full Text) (Abstract) -adrenergic-related pathway and nitric oxide β function through a In anesthetized pigs human chorionic gonadotropin increases myocardial perfusion and (PDF) (Full Text) (Abstract)
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calcium handling in porcine coronary endothelial cells by Gastrin 17
Journal of Molecular Endocrinology, 2013Co-Authors: Elena Grossini, Claudio Molinari, Lorenzo Sigaudo, Monica Biella, David Mary, Giovanni VaccaAbstract:In porcine coronary artery endothelial cells (PCAEC), Gastrin-17 has recently been found to increase nitric oxide (NO) production by the endothelial NO synthase (eNOS) isoform through cholecystokinin 1/2 (CCK1/2) receptors and the involvement of protein kinase A (PKA), PKC and the b2-adrenoreceptor-related pathway. As eNOS is the Ca 2C -dependent isoform of the enzyme, we aimed to examine the effects of Gastrin-17 on Ca 2C movements. Thus, experiments were performed in Fura-2-acetoxymethyl-ester-loaded PCAEC, where changes of cytosolic Ca 2C ([Ca 2C ]c) caused by Gastrin-17 were analysed and compared with those of CCK receptors and b2-adrenoreceptors agonists/antagonists. In addition, some experiments were performed by stimulating cells with Gastrin-17 in the presence or absence of cAMP/PKA activator/inhibitor and of phospholipase C (PLC) and Ca 2C –calmodulin dependent protein kinase II (CaMKII) blockers. The results have shown that Gastrin-17 can promote a transient increase in [Ca 2C ]c mainly originating from an intracellular pool sensitive to thapsigargin and from the extracellular space. In addition, the response of cells to Gastrin-17 was increased by the adenylyl cyclase activator and the b2-adrenoreceptor agonists and affected mainly by the CCK2 receptor agonists/antagonists. Moreover, the effects of Gastrin-17 were prevented by b2-adrenoreceptors and CaMKII blockers and the adenylyl cyclase/PKA and PLC inhibitors. Finally, in PCAEC cultured in Na C -free medium or loaded with the plasma membrane Ca 2C pump inhibitor, the Gastrin-17-evoked Ca 2C transient was long lasting. In conclusion, this study shows that Gastrin-17 affected intracellular Ca 2C homeostasis in PCAEC by both promoting a discharge of an intracellular pool and by interfering with the operation of store-dependent channels through mainly CCK2 receptors and PKA/PLC- and CaMKII-related signalling downstream of b2-adrenoreceptor stimulation.
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cck receptors related signaling involved in nitric oxide production caused by Gastrin 17 in porcine coronary endothelial cells
Molecular and Cellular Endocrinology, 2012Co-Authors: Elena Grossini, Claudio Molinari, David Mary, Philippe Primo Caimmi, Francesca Uberti, Giovanni VaccaAbstract:Abstract In anesthetized pigs Gastrin-17 increased coronary blood flow through CCK1/CCK2 receptors and β2-adrenoceptors-related nitric oxide (NO) release. Since the intracellular pathway has not been investigated the purpose of this study was to examine in coronary endothelial cells the CCK1/CCK2 receptors-related signaling involved in the effects of Gastrin-17 on NO release. Gastrin-17 caused a concentration-dependent increase of NO production (17.3–62.6%; p
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intracoronary Gastrin 17 increases cardiac perfusion and function through autonomic nervous system cck receptors and nitric oxide in anesthetized pigs
Journal of Applied Physiology, 2011Co-Authors: Elena Grossini, Claudio Molinari, David Mary, Philippe Primo Caimmi, Francesca Uberti, Giovanni VaccaAbstract:The release of gastrointestinal hormones has been reported to modulate reflex cardiovascular responses caused by gastric distension, although the role played by Gastrin 17 is as yet unknown. The pr...
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Intracoronary Gastrin 17 increases cardiac perfusion and function through autonomic nervous system, CCK receptors, and nitric oxide in anesthetized pigs
Journal of Applied Physiology, 2010Co-Authors: Elena Grossini, Claudio Molinari, David Mary, Philippe Primo Caimmi, Francesca Uberti, Giovanni VaccaAbstract:The release of gastrointestinal hormones has been reported to modulate reflex cardiovascular responses caused by gastric distension, although the role played by Gastrin 17 is as yet unknown. The present study was therefore planned to determine the primary in vivo effect of Gastrin 17 on coronary blood flow and cardiac function and the involvement of autonomic nervous system, CCK1/2 receptors, and nitric oxide (NO). In 40 anesthetized pigs, Gastrin 17 was infused into the left anterior descending coronary artery at constant heart rate and arterial blood pressure. In 35 of the 40 pigs, the mechanisms of the observed hemodynamic responses were analyzed by repeating Gastrin 17 infusion after autonomic nervous system and NO blockade, and after specific CCK receptors agonists/antagonists administration. Intracoronary Gastrin 17 administration caused dose-related increases of both coronary blood flow and cardiac function. The intracoronary co-administration of CCK33/pentaGastrin and Gastrin 17 potentiated the co...
David Mary - One of the best experts on this subject based on the ideXlab platform.
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calcium handling in porcine coronary endothelial cells by Gastrin 17
Journal of Molecular Endocrinology, 2013Co-Authors: Elena Grossini, Claudio Molinari, Lorenzo Sigaudo, Monica Biella, David Mary, Giovanni VaccaAbstract:In porcine coronary artery endothelial cells (PCAEC), Gastrin-17 has recently been found to increase nitric oxide (NO) production by the endothelial NO synthase (eNOS) isoform through cholecystokinin 1/2 (CCK1/2) receptors and the involvement of protein kinase A (PKA), PKC and the b2-adrenoreceptor-related pathway. As eNOS is the Ca 2C -dependent isoform of the enzyme, we aimed to examine the effects of Gastrin-17 on Ca 2C movements. Thus, experiments were performed in Fura-2-acetoxymethyl-ester-loaded PCAEC, where changes of cytosolic Ca 2C ([Ca 2C ]c) caused by Gastrin-17 were analysed and compared with those of CCK receptors and b2-adrenoreceptors agonists/antagonists. In addition, some experiments were performed by stimulating cells with Gastrin-17 in the presence or absence of cAMP/PKA activator/inhibitor and of phospholipase C (PLC) and Ca 2C –calmodulin dependent protein kinase II (CaMKII) blockers. The results have shown that Gastrin-17 can promote a transient increase in [Ca 2C ]c mainly originating from an intracellular pool sensitive to thapsigargin and from the extracellular space. In addition, the response of cells to Gastrin-17 was increased by the adenylyl cyclase activator and the b2-adrenoreceptor agonists and affected mainly by the CCK2 receptor agonists/antagonists. Moreover, the effects of Gastrin-17 were prevented by b2-adrenoreceptors and CaMKII blockers and the adenylyl cyclase/PKA and PLC inhibitors. Finally, in PCAEC cultured in Na C -free medium or loaded with the plasma membrane Ca 2C pump inhibitor, the Gastrin-17-evoked Ca 2C transient was long lasting. In conclusion, this study shows that Gastrin-17 affected intracellular Ca 2C homeostasis in PCAEC by both promoting a discharge of an intracellular pool and by interfering with the operation of store-dependent channels through mainly CCK2 receptors and PKA/PLC- and CaMKII-related signalling downstream of b2-adrenoreceptor stimulation.
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cck receptors related signaling involved in nitric oxide production caused by Gastrin 17 in porcine coronary endothelial cells
Molecular and Cellular Endocrinology, 2012Co-Authors: Elena Grossini, Claudio Molinari, David Mary, Philippe Primo Caimmi, Francesca Uberti, Giovanni VaccaAbstract:Abstract In anesthetized pigs Gastrin-17 increased coronary blood flow through CCK1/CCK2 receptors and β2-adrenoceptors-related nitric oxide (NO) release. Since the intracellular pathway has not been investigated the purpose of this study was to examine in coronary endothelial cells the CCK1/CCK2 receptors-related signaling involved in the effects of Gastrin-17 on NO release. Gastrin-17 caused a concentration-dependent increase of NO production (17.3–62.6%; p
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intracoronary Gastrin 17 increases cardiac perfusion and function through autonomic nervous system cck receptors and nitric oxide in anesthetized pigs
Journal of Applied Physiology, 2011Co-Authors: Elena Grossini, Claudio Molinari, David Mary, Philippe Primo Caimmi, Francesca Uberti, Giovanni VaccaAbstract:The release of gastrointestinal hormones has been reported to modulate reflex cardiovascular responses caused by gastric distension, although the role played by Gastrin 17 is as yet unknown. The pr...
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Intracoronary Gastrin 17 increases cardiac perfusion and function through autonomic nervous system, CCK receptors, and nitric oxide in anesthetized pigs
Journal of Applied Physiology, 2010Co-Authors: Elena Grossini, Claudio Molinari, David Mary, Philippe Primo Caimmi, Francesca Uberti, Giovanni VaccaAbstract:The release of gastrointestinal hormones has been reported to modulate reflex cardiovascular responses caused by gastric distension, although the role played by Gastrin 17 is as yet unknown. The present study was therefore planned to determine the primary in vivo effect of Gastrin 17 on coronary blood flow and cardiac function and the involvement of autonomic nervous system, CCK1/2 receptors, and nitric oxide (NO). In 40 anesthetized pigs, Gastrin 17 was infused into the left anterior descending coronary artery at constant heart rate and arterial blood pressure. In 35 of the 40 pigs, the mechanisms of the observed hemodynamic responses were analyzed by repeating Gastrin 17 infusion after autonomic nervous system and NO blockade, and after specific CCK receptors agonists/antagonists administration. Intracoronary Gastrin 17 administration caused dose-related increases of both coronary blood flow and cardiac function. The intracoronary co-administration of CCK33/pentaGastrin and Gastrin 17 potentiated the co...
