The Experts below are selected from a list of 9381 Experts worldwide ranked by ideXlab platform
Ilene K Gipson - One of the best experts on this subject based on the ideXlab platform.
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spdef null mice lack conjunctival goblet cells and provide a model of dry eye
American Journal of Pathology, 2013Co-Authors: Christina K Marko, Hans Clevers, Balaraj B Menon, Ilene K GipsonAbstract:Goblet cell numbers decrease within the conjunctival Epithelium in drying and cicatrizing ocular surface diseases. Factors regulating goblet cell differentiation in conjunctival Epithelium are unknown. Recent data indicate that the transcription factor SAM-pointed domain epithelial-specific transcription factor (Spdef) is essential for goblet cell differentiation in tracheobronchial and Gastrointestinal Epithelium of mice. Using Spdef−/− mice, we determined that Spdef is required for conjunctival goblet cell differentiation and that Spdef−/− mice, which lack conjunctival goblet cells, have significantly increased corneal surface fluorescein staining and tear volume, a phenotype consistent with dry eye. Microarray analysis of conjunctival Epithelium in Spdef−/− mice revealed down-regulation of goblet cell–specific genes (Muc5ac, Tff1, Gcnt3). Up-regulated genes included epithelial cell differentiation/keratinization genes (Sprr2h, Tgm1) and proinflammatory genes (Il1-α, Il-1β, Tnf-α), all of which are up-regulated in dry eye. Interestingly, four Wnt pathway genes were down-regulated. SPDEF expression was significantly decreased in the conjunctival Epithelium of Sjogren syndrome patients with dry eye and decreased goblet cell mucin expression. These data demonstrate that Spdef is required for conjunctival goblet cell differentiation and down-regulation of SPDEF may play a role in human dry eye with goblet cell loss. Spdef−/− mice have an ocular surface phenotype similar to that in moderate dry eye, providing a new, more convenient model for the disease.
Christina K Marko - One of the best experts on this subject based on the ideXlab platform.
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spdef null mice lack conjunctival goblet cells and provide a model of dry eye
American Journal of Pathology, 2013Co-Authors: Christina K Marko, Hans Clevers, Balaraj B Menon, Ilene K GipsonAbstract:Goblet cell numbers decrease within the conjunctival Epithelium in drying and cicatrizing ocular surface diseases. Factors regulating goblet cell differentiation in conjunctival Epithelium are unknown. Recent data indicate that the transcription factor SAM-pointed domain epithelial-specific transcription factor (Spdef) is essential for goblet cell differentiation in tracheobronchial and Gastrointestinal Epithelium of mice. Using Spdef−/− mice, we determined that Spdef is required for conjunctival goblet cell differentiation and that Spdef−/− mice, which lack conjunctival goblet cells, have significantly increased corneal surface fluorescein staining and tear volume, a phenotype consistent with dry eye. Microarray analysis of conjunctival Epithelium in Spdef−/− mice revealed down-regulation of goblet cell–specific genes (Muc5ac, Tff1, Gcnt3). Up-regulated genes included epithelial cell differentiation/keratinization genes (Sprr2h, Tgm1) and proinflammatory genes (Il1-α, Il-1β, Tnf-α), all of which are up-regulated in dry eye. Interestingly, four Wnt pathway genes were down-regulated. SPDEF expression was significantly decreased in the conjunctival Epithelium of Sjogren syndrome patients with dry eye and decreased goblet cell mucin expression. These data demonstrate that Spdef is required for conjunctival goblet cell differentiation and down-regulation of SPDEF may play a role in human dry eye with goblet cell loss. Spdef−/− mice have an ocular surface phenotype similar to that in moderate dry eye, providing a new, more convenient model for the disease.
Hans Clevers - One of the best experts on this subject based on the ideXlab platform.
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spdef null mice lack conjunctival goblet cells and provide a model of dry eye
American Journal of Pathology, 2013Co-Authors: Christina K Marko, Hans Clevers, Balaraj B Menon, Ilene K GipsonAbstract:Goblet cell numbers decrease within the conjunctival Epithelium in drying and cicatrizing ocular surface diseases. Factors regulating goblet cell differentiation in conjunctival Epithelium are unknown. Recent data indicate that the transcription factor SAM-pointed domain epithelial-specific transcription factor (Spdef) is essential for goblet cell differentiation in tracheobronchial and Gastrointestinal Epithelium of mice. Using Spdef−/− mice, we determined that Spdef is required for conjunctival goblet cell differentiation and that Spdef−/− mice, which lack conjunctival goblet cells, have significantly increased corneal surface fluorescein staining and tear volume, a phenotype consistent with dry eye. Microarray analysis of conjunctival Epithelium in Spdef−/− mice revealed down-regulation of goblet cell–specific genes (Muc5ac, Tff1, Gcnt3). Up-regulated genes included epithelial cell differentiation/keratinization genes (Sprr2h, Tgm1) and proinflammatory genes (Il1-α, Il-1β, Tnf-α), all of which are up-regulated in dry eye. Interestingly, four Wnt pathway genes were down-regulated. SPDEF expression was significantly decreased in the conjunctival Epithelium of Sjogren syndrome patients with dry eye and decreased goblet cell mucin expression. These data demonstrate that Spdef is required for conjunctival goblet cell differentiation and down-regulation of SPDEF may play a role in human dry eye with goblet cell loss. Spdef−/− mice have an ocular surface phenotype similar to that in moderate dry eye, providing a new, more convenient model for the disease.
Balaraj B Menon - One of the best experts on this subject based on the ideXlab platform.
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spdef null mice lack conjunctival goblet cells and provide a model of dry eye
American Journal of Pathology, 2013Co-Authors: Christina K Marko, Hans Clevers, Balaraj B Menon, Ilene K GipsonAbstract:Goblet cell numbers decrease within the conjunctival Epithelium in drying and cicatrizing ocular surface diseases. Factors regulating goblet cell differentiation in conjunctival Epithelium are unknown. Recent data indicate that the transcription factor SAM-pointed domain epithelial-specific transcription factor (Spdef) is essential for goblet cell differentiation in tracheobronchial and Gastrointestinal Epithelium of mice. Using Spdef−/− mice, we determined that Spdef is required for conjunctival goblet cell differentiation and that Spdef−/− mice, which lack conjunctival goblet cells, have significantly increased corneal surface fluorescein staining and tear volume, a phenotype consistent with dry eye. Microarray analysis of conjunctival Epithelium in Spdef−/− mice revealed down-regulation of goblet cell–specific genes (Muc5ac, Tff1, Gcnt3). Up-regulated genes included epithelial cell differentiation/keratinization genes (Sprr2h, Tgm1) and proinflammatory genes (Il1-α, Il-1β, Tnf-α), all of which are up-regulated in dry eye. Interestingly, four Wnt pathway genes were down-regulated. SPDEF expression was significantly decreased in the conjunctival Epithelium of Sjogren syndrome patients with dry eye and decreased goblet cell mucin expression. These data demonstrate that Spdef is required for conjunctival goblet cell differentiation and down-regulation of SPDEF may play a role in human dry eye with goblet cell loss. Spdef−/− mice have an ocular surface phenotype similar to that in moderate dry eye, providing a new, more convenient model for the disease.
Robert A Soslow - One of the best experts on this subject based on the ideXlab platform.
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Gastrointestinal adenocarcinoma arising in a mature cystic teratoma of the ovary
Gynecologic Oncology, 2004Co-Authors: Douglas A Levine, Jeannine A Villella, Elizabeth A Poynor, Robert A SoslowAbstract:Abstract Background . Mature cystic teratoma of the ovary (MCTO) is the most common ovarian germ cell neoplasm and is usually diagnosed in early adulthood. Malignant transformation is rare, occurring in approximately 2% of all cases. Though malignant transformation can occur from any of the embryonic germ layers, the most common malignancy arising in these otherwise benign tumors is squamous cell carcinoma. Case. We present a patient with a MCTO where malignant transformation of Gastrointestinal Epithelium resulted in moderately differentiated adenocarcinoma. After 3 years of follow-up, she remains free of disease. Conclusion . Although Gastrointestinal Epithelium is often found in MCTOs, adenocarcinoma arising from this cell type is uncommon. This is the third reported case of adenocarcinoma arising in Gastrointestinal Epithelium of a MCTO.
