The Experts below are selected from a list of 270 Experts worldwide ranked by ideXlab platform
Janice Brittondavidian - One of the best experts on this subject based on the ideXlab platform.
-
starch Gel Electrophoresis in vertebrates
Methods in Enzymology, 1993Co-Authors: Janice BrittondavidianAbstract:Publisher Summary This chapter discusses starch Gel Electrophoresis in vertebrates. Starch Gel Electrophoresis is used successfully in population genetics, systematics, phylogeny, and population structure studies. The chapter presents guidelines for sample and Gel preparation and lists the protein stains and Electrophoresis buffers commonly used for a variety of vertebrate species. The standard equipment needed for protein Electrophoresis consists of a refrigerated centrifuge, a deep freezer (-70°C) or a liquid nitrogen container, a crushed ice machine, a pH meter, an incubator, an electric barrel homogenizer, a power pack, ultraviolet (UV) light source, and storage boxes for 1.5-ml Eppendorf microtubes. Most of the specific electrophoretic equipment (Gel molds, electrode trays, cutting plates, Gel slicer) can be made in the laboratory from plexiglass sheets and commercially available material. Stain boxes with lids are required. As most reagents used in Electrophoresis are highly toxic, all necessary precautions must be taken, and rubber gloves should be worn when handling acrylamide and stains.
Vladimir Besada - One of the best experts on this subject based on the ideXlab platform.
-
sodium dodecyl sulfate free Gel Electrophoresis electroelution sorting for peptide fractionation
IEEE Journal of Solid-state Circuits, 2019Co-Authors: Yassel Ramos, Annia Gonzalez, Patricia Sosaacosta, Yasset Perezriverol, Yairet Garcia, Lila Castellanosserra, Aniel Sanchez, L. Gonzalez, Vladimir BesadaAbstract:Shotgun proteomics based on peptide fractionation by using liquid chromatography has become the common procedure for proteomic studies, although in the very beginning of the field, protein separation by using Electrophoresis was the main tool. Nonetheless, during the last two decades, the electrophoretic techniques for peptide mixtures fractionation have evolved as a result of relevant technological improvements. We also proposed the combination of sodium dodecyl sulfate polyacrylamide Gel Electrophoresis for protein fractionation and sodium dodecyl sulfate free polyacrylamide Gel Electrophoresis for peptide separation as a novel procedure for proteomic studies. Here, we present an optimized device for sodium dodecyl sulfate free polyacrylamide Gel Electrophoresis improving peptide recoveries respect to the established electrophoretic technique off Gel Electrophoresis meanwhile conserving the excellent resolution described for the former technique in slab Gel based systems. The device simultaneously allows the separation and the collection of fractionated peptides in solution.
Yassel Ramos - One of the best experts on this subject based on the ideXlab platform.
-
sodium dodecyl sulfate free Gel Electrophoresis electroelution sorting for peptide fractionation
IEEE Journal of Solid-state Circuits, 2019Co-Authors: Yassel Ramos, Annia Gonzalez, Patricia Sosaacosta, Yasset Perezriverol, Yairet Garcia, Lila Castellanosserra, Aniel Sanchez, L. Gonzalez, Vladimir BesadaAbstract:Shotgun proteomics based on peptide fractionation by using liquid chromatography has become the common procedure for proteomic studies, although in the very beginning of the field, protein separation by using Electrophoresis was the main tool. Nonetheless, during the last two decades, the electrophoretic techniques for peptide mixtures fractionation have evolved as a result of relevant technological improvements. We also proposed the combination of sodium dodecyl sulfate polyacrylamide Gel Electrophoresis for protein fractionation and sodium dodecyl sulfate free polyacrylamide Gel Electrophoresis for peptide separation as a novel procedure for proteomic studies. Here, we present an optimized device for sodium dodecyl sulfate free polyacrylamide Gel Electrophoresis improving peptide recoveries respect to the established electrophoretic technique off Gel Electrophoresis meanwhile conserving the excellent resolution described for the former technique in slab Gel based systems. The device simultaneously allows the separation and the collection of fractionated peptides in solution.
L. Gonzalez - One of the best experts on this subject based on the ideXlab platform.
-
sodium dodecyl sulfate free Gel Electrophoresis electroelution sorting for peptide fractionation
IEEE Journal of Solid-state Circuits, 2019Co-Authors: Yassel Ramos, Annia Gonzalez, Patricia Sosaacosta, Yasset Perezriverol, Yairet Garcia, Lila Castellanosserra, Aniel Sanchez, L. Gonzalez, Vladimir BesadaAbstract:Shotgun proteomics based on peptide fractionation by using liquid chromatography has become the common procedure for proteomic studies, although in the very beginning of the field, protein separation by using Electrophoresis was the main tool. Nonetheless, during the last two decades, the electrophoretic techniques for peptide mixtures fractionation have evolved as a result of relevant technological improvements. We also proposed the combination of sodium dodecyl sulfate polyacrylamide Gel Electrophoresis for protein fractionation and sodium dodecyl sulfate free polyacrylamide Gel Electrophoresis for peptide separation as a novel procedure for proteomic studies. Here, we present an optimized device for sodium dodecyl sulfate free polyacrylamide Gel Electrophoresis improving peptide recoveries respect to the established electrophoretic technique off Gel Electrophoresis meanwhile conserving the excellent resolution described for the former technique in slab Gel based systems. The device simultaneously allows the separation and the collection of fractionated peptides in solution.
Annia Gonzalez - One of the best experts on this subject based on the ideXlab platform.
-
sodium dodecyl sulfate free Gel Electrophoresis electroelution sorting for peptide fractionation
IEEE Journal of Solid-state Circuits, 2019Co-Authors: Yassel Ramos, Annia Gonzalez, Patricia Sosaacosta, Yasset Perezriverol, Yairet Garcia, Lila Castellanosserra, Aniel Sanchez, L. Gonzalez, Vladimir BesadaAbstract:Shotgun proteomics based on peptide fractionation by using liquid chromatography has become the common procedure for proteomic studies, although in the very beginning of the field, protein separation by using Electrophoresis was the main tool. Nonetheless, during the last two decades, the electrophoretic techniques for peptide mixtures fractionation have evolved as a result of relevant technological improvements. We also proposed the combination of sodium dodecyl sulfate polyacrylamide Gel Electrophoresis for protein fractionation and sodium dodecyl sulfate free polyacrylamide Gel Electrophoresis for peptide separation as a novel procedure for proteomic studies. Here, we present an optimized device for sodium dodecyl sulfate free polyacrylamide Gel Electrophoresis improving peptide recoveries respect to the established electrophoretic technique off Gel Electrophoresis meanwhile conserving the excellent resolution described for the former technique in slab Gel based systems. The device simultaneously allows the separation and the collection of fractionated peptides in solution.
