The Experts below are selected from a list of 28182 Experts worldwide ranked by ideXlab platform
Quanbin Zhang - One of the best experts on this subject based on the ideXlab platform.
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structural analysis of a heteropolysaccharide from saccharina japonica by electrospray mass spectrometry in tandem with collision induced dissociation tandem mass spectrometry esi cid ms ms
Marine Drugs, 2012Co-Authors: Weihua Jin, Jing Wang, Sumei Ren, Ni Song, Quanbin ZhangAbstract:A fucoidan extracted from Saccharina japonica was fractionated by anion exchange Chromatography. The most complex fraction F0.5 was degraded by dilute sulphuric acid and then separated by use of an activated carbon column. Fraction Y1 was fractionated by anion exchange and Gel Filtration Chromatography while Fraction Y2 was fractionated by Gel Filtration Chromatography. The fractions were determined by ESI-MS and analyzed by ESI-CID-MS/MS. It was concluded that F0.5 had a backbone of alternating 4-linked GlcA and 2-linked Man with the first Man residue from the nonreducing end accidentally sulfated at C6. In addition, F0.5 had a 3-linked glucuronan, in accordance with a previous report by NMR. Some other structural characteristics included GlcA 1→3 Man 1→4 GlcA, Man 1→3 GlcA 1→4 GlcA, Fuc 1→4 GlcA and Fuc 1→3 Fuc. Finally, it was shown that fucose was sulfated at C2 or C4 while galactose was sulfated at C2, C4 or C6.
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Structural Analysis of a Heteropolysaccharide from <em>Saccharina japonica</em> by Electrospray Mass Spectrometry in Tandem with Collision-Induced Dissociation Tandem Mass Spectrometry (ESI-CID-MS/MS)
MDPI AG, 2012Co-Authors: Weihua Jin, Jing Wang, Sumei Ren, Ni Song, Quanbin ZhangAbstract:A fucoidan extracted from <em>Saccharina japonica</em> was fractionated by anion exchange Chromatography. The most complex fraction F0.5 was degraded by dilute sulphuric acid and then separated by use of an activated carbon column. Fraction Y1 was fractionated by anion exchange and Gel Filtration Chromatography while Fraction Y2 was fractionated by Gel Filtration Chromatography. The fractions were determined by ESI-MS and analyzed by ESI-CID-MS/MS. It was concluded that F0.5 had a backbone of alternating 4-linked GlcA and 2-linked Man with the first Man residue from the nonreducing end accidentally sulfated at C6. In addition, F0.5 had a 3-linked glucuronan, in accordance with a previous report by NMR. Some other structural characteristics included GlcA 1→3 Man 1→4 GlcA, Man 1→3 GlcA 1→4 GlcA, Fuc 1→4 GlcA and Fuc 1→3 Fuc. Finally, it was shown that fucose was sulfated at C2 or C4 while galactose was sulfated at C2, C4 or C6
Ashis K Mukherjee - One of the best experts on this subject based on the ideXlab platform.
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proteomic analysis and immuno profiling of eastern india russell s viper daboia russelii venom correlation between rvv composition and clinical manifestations post rv bite
Journal of Proteome Research, 2018Co-Authors: Bhargab Kalita, Aparup Patra, Anjali Das, Ashis K MukherjeeAbstract:The proteomes of Russell’s viper venom (RVV) from Burdwan (RVV B) and Nadia (RVV N), the two districts of West Bengal, eastern India (EI), were investigated by Gel-Filtration Chromatography (GFC) followed by tandem mass spectrometry of tryptic fragments of the fractions. A total of 73 and 69 proteins belonging to 15 snake venom protein families were identified in RVV B and RVV N, respectively, by MS/MS search against Viperidae (taxid 8689) protein entries of the nonredundant NCBI database. The minor differences in venom composition of both the EI RV were established unequivocally by their biochemical and pharmacological properties and by SDS-PAGE, Gel Filtration Chromatography, and LC-MS/MS analyses. The composition of EI RVVs was well correlated with published reports on the pathophysiology of RV-envenomed patients from this part of the country. Venom–antivenom cross-reactivity determined by ELISA, Western blotting, and antivenomics approaches demonstrated poor recognition of low molecular mass (<20 kDa)...
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Proteomic Analysis and Immuno-Profiling of Eastern India Russell’s Viper (Daboia russelii) Venom: Correlation between RVV Composition and Clinical Manifestations Post RV Bite
2018Co-Authors: Bhargab Kalita, Aparup Patra, Anjali Das, Ashis K MukherjeeAbstract:The proteomes of Russell’s viper venom (RVV) from Burdwan (RVV B) and Nadia (RVV N), the two districts of West Bengal, eastern India (EI), were investigated by Gel-Filtration Chromatography (GFC) followed by tandem mass spectrometry of tryptic fragments of the fractions. A total of 73 and 69 proteins belonging to 15 snake venom protein families were identified in RVV B and RVV N, respectively, by MS/MS search against Viperidae (taxid 8689) protein entries of the nonredundant NCBI database. The minor differences in venom composition of both the EI RV were established unequivocally by their biochemical and pharmacological properties and by SDS-PAGE, Gel Filtration Chromatography, and LC-MS/MS analyses. The composition of EI RVVs was well correlated with published reports on the pathophysiology of RV-envenomed patients from this part of the country. Venom–antivenom cross-reactivity determined by ELISA, Western blotting, and antivenomics approaches demonstrated poor recognition of low molecular mass (
Weihua Jin - One of the best experts on this subject based on the ideXlab platform.
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structural analysis of a heteropolysaccharide from saccharina japonica by electrospray mass spectrometry in tandem with collision induced dissociation tandem mass spectrometry esi cid ms ms
Marine Drugs, 2012Co-Authors: Weihua Jin, Jing Wang, Sumei Ren, Ni Song, Quanbin ZhangAbstract:A fucoidan extracted from Saccharina japonica was fractionated by anion exchange Chromatography. The most complex fraction F0.5 was degraded by dilute sulphuric acid and then separated by use of an activated carbon column. Fraction Y1 was fractionated by anion exchange and Gel Filtration Chromatography while Fraction Y2 was fractionated by Gel Filtration Chromatography. The fractions were determined by ESI-MS and analyzed by ESI-CID-MS/MS. It was concluded that F0.5 had a backbone of alternating 4-linked GlcA and 2-linked Man with the first Man residue from the nonreducing end accidentally sulfated at C6. In addition, F0.5 had a 3-linked glucuronan, in accordance with a previous report by NMR. Some other structural characteristics included GlcA 1→3 Man 1→4 GlcA, Man 1→3 GlcA 1→4 GlcA, Fuc 1→4 GlcA and Fuc 1→3 Fuc. Finally, it was shown that fucose was sulfated at C2 or C4 while galactose was sulfated at C2, C4 or C6.
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Structural Analysis of a Heteropolysaccharide from <em>Saccharina japonica</em> by Electrospray Mass Spectrometry in Tandem with Collision-Induced Dissociation Tandem Mass Spectrometry (ESI-CID-MS/MS)
MDPI AG, 2012Co-Authors: Weihua Jin, Jing Wang, Sumei Ren, Ni Song, Quanbin ZhangAbstract:A fucoidan extracted from <em>Saccharina japonica</em> was fractionated by anion exchange Chromatography. The most complex fraction F0.5 was degraded by dilute sulphuric acid and then separated by use of an activated carbon column. Fraction Y1 was fractionated by anion exchange and Gel Filtration Chromatography while Fraction Y2 was fractionated by Gel Filtration Chromatography. The fractions were determined by ESI-MS and analyzed by ESI-CID-MS/MS. It was concluded that F0.5 had a backbone of alternating 4-linked GlcA and 2-linked Man with the first Man residue from the nonreducing end accidentally sulfated at C6. In addition, F0.5 had a 3-linked glucuronan, in accordance with a previous report by NMR. Some other structural characteristics included GlcA 1→3 Man 1→4 GlcA, Man 1→3 GlcA 1→4 GlcA, Fuc 1→4 GlcA and Fuc 1→3 Fuc. Finally, it was shown that fucose was sulfated at C2 or C4 while galactose was sulfated at C2, C4 or C6
Ni Song - One of the best experts on this subject based on the ideXlab platform.
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structural analysis of a heteropolysaccharide from saccharina japonica by electrospray mass spectrometry in tandem with collision induced dissociation tandem mass spectrometry esi cid ms ms
Marine Drugs, 2012Co-Authors: Weihua Jin, Jing Wang, Sumei Ren, Ni Song, Quanbin ZhangAbstract:A fucoidan extracted from Saccharina japonica was fractionated by anion exchange Chromatography. The most complex fraction F0.5 was degraded by dilute sulphuric acid and then separated by use of an activated carbon column. Fraction Y1 was fractionated by anion exchange and Gel Filtration Chromatography while Fraction Y2 was fractionated by Gel Filtration Chromatography. The fractions were determined by ESI-MS and analyzed by ESI-CID-MS/MS. It was concluded that F0.5 had a backbone of alternating 4-linked GlcA and 2-linked Man with the first Man residue from the nonreducing end accidentally sulfated at C6. In addition, F0.5 had a 3-linked glucuronan, in accordance with a previous report by NMR. Some other structural characteristics included GlcA 1→3 Man 1→4 GlcA, Man 1→3 GlcA 1→4 GlcA, Fuc 1→4 GlcA and Fuc 1→3 Fuc. Finally, it was shown that fucose was sulfated at C2 or C4 while galactose was sulfated at C2, C4 or C6.
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Structural Analysis of a Heteropolysaccharide from <em>Saccharina japonica</em> by Electrospray Mass Spectrometry in Tandem with Collision-Induced Dissociation Tandem Mass Spectrometry (ESI-CID-MS/MS)
MDPI AG, 2012Co-Authors: Weihua Jin, Jing Wang, Sumei Ren, Ni Song, Quanbin ZhangAbstract:A fucoidan extracted from <em>Saccharina japonica</em> was fractionated by anion exchange Chromatography. The most complex fraction F0.5 was degraded by dilute sulphuric acid and then separated by use of an activated carbon column. Fraction Y1 was fractionated by anion exchange and Gel Filtration Chromatography while Fraction Y2 was fractionated by Gel Filtration Chromatography. The fractions were determined by ESI-MS and analyzed by ESI-CID-MS/MS. It was concluded that F0.5 had a backbone of alternating 4-linked GlcA and 2-linked Man with the first Man residue from the nonreducing end accidentally sulfated at C6. In addition, F0.5 had a 3-linked glucuronan, in accordance with a previous report by NMR. Some other structural characteristics included GlcA 1→3 Man 1→4 GlcA, Man 1→3 GlcA 1→4 GlcA, Fuc 1→4 GlcA and Fuc 1→3 Fuc. Finally, it was shown that fucose was sulfated at C2 or C4 while galactose was sulfated at C2, C4 or C6
Jing Wang - One of the best experts on this subject based on the ideXlab platform.
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structural analysis of a heteropolysaccharide from saccharina japonica by electrospray mass spectrometry in tandem with collision induced dissociation tandem mass spectrometry esi cid ms ms
Marine Drugs, 2012Co-Authors: Weihua Jin, Jing Wang, Sumei Ren, Ni Song, Quanbin ZhangAbstract:A fucoidan extracted from Saccharina japonica was fractionated by anion exchange Chromatography. The most complex fraction F0.5 was degraded by dilute sulphuric acid and then separated by use of an activated carbon column. Fraction Y1 was fractionated by anion exchange and Gel Filtration Chromatography while Fraction Y2 was fractionated by Gel Filtration Chromatography. The fractions were determined by ESI-MS and analyzed by ESI-CID-MS/MS. It was concluded that F0.5 had a backbone of alternating 4-linked GlcA and 2-linked Man with the first Man residue from the nonreducing end accidentally sulfated at C6. In addition, F0.5 had a 3-linked glucuronan, in accordance with a previous report by NMR. Some other structural characteristics included GlcA 1→3 Man 1→4 GlcA, Man 1→3 GlcA 1→4 GlcA, Fuc 1→4 GlcA and Fuc 1→3 Fuc. Finally, it was shown that fucose was sulfated at C2 or C4 while galactose was sulfated at C2, C4 or C6.
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Structural Analysis of a Heteropolysaccharide from <em>Saccharina japonica</em> by Electrospray Mass Spectrometry in Tandem with Collision-Induced Dissociation Tandem Mass Spectrometry (ESI-CID-MS/MS)
MDPI AG, 2012Co-Authors: Weihua Jin, Jing Wang, Sumei Ren, Ni Song, Quanbin ZhangAbstract:A fucoidan extracted from <em>Saccharina japonica</em> was fractionated by anion exchange Chromatography. The most complex fraction F0.5 was degraded by dilute sulphuric acid and then separated by use of an activated carbon column. Fraction Y1 was fractionated by anion exchange and Gel Filtration Chromatography while Fraction Y2 was fractionated by Gel Filtration Chromatography. The fractions were determined by ESI-MS and analyzed by ESI-CID-MS/MS. It was concluded that F0.5 had a backbone of alternating 4-linked GlcA and 2-linked Man with the first Man residue from the nonreducing end accidentally sulfated at C6. In addition, F0.5 had a 3-linked glucuronan, in accordance with a previous report by NMR. Some other structural characteristics included GlcA 1→3 Man 1→4 GlcA, Man 1→3 GlcA 1→4 GlcA, Fuc 1→4 GlcA and Fuc 1→3 Fuc. Finally, it was shown that fucose was sulfated at C2 or C4 while galactose was sulfated at C2, C4 or C6
