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Sungkoo Kim - One of the best experts on this subject based on the ideXlab platform.
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evaluation of 2 3 butanediol production from red seaweed Gelidium amansii hydrolysates using engineered saccharomyces cerevisiae
Journal of Microbiology and Biotechnology, 2020Co-Authors: Jinho Seo, Gwitaek Jeong, Sungkoo KimAbstract:Hyper-thermal (HT) acid hydrolysis of red seaweed Gelidium amansii was performed using 12% (w/v) slurry and an acid mix concentration of 180 mM at 150°C for 10 min. Enzymatic saccharification when using a combination of Celluclast 1.5 L and CTec2 at a dose of 16 U/ml led to the production of 12.0 g/l of reducing sugar with an efficiency of enzymatic saccharification of 13.2%. After the enzymatic saccharification, 2,3-butanediol (2,3-BD) fermentation was carried out using an engineered S. cerevisiae strain. The use of HT acid-hydrolyzed medium with 1.9 g/l of 5-hydroxymethylfurfural showed a reduction in the lag time from 48 to 24 h. The 2,3-BD concentration and yield coefficient at 72 h were 14.8 g/l and 0.30, respectively. Therefore, HT acid hydrolysis and the use of the engineered S. cerevisiae strain can enhance the overall 2,3-BD yields from G. amansii seaweed.
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evaluation of 2 3 butanediol production from red seaweed Gelidium amansii hydrolysates using engineered saccharomyces cerevisiae
Journal of Microbiology and Biotechnology, 2020Co-Authors: Jinho Seo, Gwitaek Jeong, Sungkoo KimAbstract:Hyper thermal (HT) acid hydrolysis of red seaweed Gelidium amansii was performed using 12% (w/v) slurry and an acid mix concentration of 180 mM at 150 °C for 10 min. Enzymatic saccharification when using a combination of Celluclast 1.5 L and Ctec2 at a dose of 16 U/mL led to the production of 12.0 g/L of reducing sugar with an the efficiency of enzymatic saccharification (Es) of 13.2%. After the enzymatic saccharification, 2,3-butanediol (2,3-BD) fermentation was carried out using an engineered S. cerevisiae strain. The use of HT acid-hydrolyzed medium with 1.9 g/L of 5-HMF showed a reduction in the lag time from 48 to 24 h. The 2,3-BD concentration and yield at 72 h were 14.8 g/L and 0.30, respectively. Therefore, HT acid hydrolysis and the use of the engineered S. cerevisiae strain can enhance the overall 2,3-BD yields from G. amansii seaweed.
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detoxification of hydrolysates of the red seaweed Gelidium amansii for improved bioethanol production
Applied Biochemistry and Biotechnology, 2019Co-Authors: Trung Hau Nguyen, Gwitaek Jeong, In Yung Sunwoo, Sungkoo KimAbstract:In this study, bioethanol was produced from the seaweed Gelidium amansii as biomass through separate hydrolysis and fermentation (SHF) processes. The SHF processes examined in this study include thermal acid hydrolysis pretreatment, enzymatic saccharification, detoxification, and fermentation. Thermal acid hydrolysis pretreatment was conducted using H2SO4, with a slurry content of 8–16% and treatment time of 15–75 min. The optimal conditions for thermal acid hydrolysis pretreatment were 12% (w/v) seaweed slurry content and 180 mM H2SO4 at 121 °C for 45 min, at which 26.1 g/L galactose and 6.8 g/L glucose were produced. A monosaccharide (mainly glucose) was also obtained from the enzymatic saccharification of thermal acid hydrolysate using 16 U/mL Celluclast 1.5 L enzyme at 45 °C for 36 h. Detoxification was performed using the adsorption method with activated carbon, the overliming method with Ca (OH)2, and the ion exchange method with polyethyleneimine. Among those detoxification methods, activated carbon showed the best performance for hydroxymethylfurfural removal. Ethanol fermentation was performed using 12% (w/v) seaweed hydrolysate with Saccharomyces cerevisiae adapted to galactose as well as various detoxification treatments.
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improved fermentation performance to produce bioethanol from Gelidium amansii using pichia stipitis adapted to galactose
Bioprocess and Biosystems Engineering, 2018Co-Authors: Pailin Sukwong, Gwitaek Jeong, In Yung Sunwoo, Sumate Tantratian, Sungkoo KimAbstract:This study employed a statistical method to obtain optimal hyper thermal acid hydrolysis conditions using Gelidium amansii (red seaweed) as a source of biomass. The optimal hyper thermal acid hydrolysis using G. amansii as biomass was determined as 12% (w/v) slurry content, 358.3 mM H2SO4, and temperature of 142.6 °C for 11 min. After hyper thermal acid hydrolysis, enzymatic saccharification was carried out. The total monosaccharide concentration was 45.1 g/L, 72.2% of the theoretical value of the total fermentable monosaccharides of 62.4 g/L based on 120 g dry weight/L in the G. amansii slurry. To increase ethanol production, 3.8 g/L 5-hydroxymethylfurfural (HMF) in the hydrolysate was removed by treatment with 3.5% (w/v) activated carbon for 2 min and fermented with Pichia stipitis adapted to high galactose concentrations via separate hydrolysis and fermentation. With complete HMF removal and the use of P. stipitis adapted to high galactose concentrations, 22 g/L ethanol was produced (yield 0.50). Fermentation with total HMF removal and yeast adapted to high galactose concentrations increased the fermentation performance and decreased the fermentation time from 96 to 36 h compared to traditional fermentation.
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hyper thermal acid hydrolysis and adsorption treatment of red seaweed Gelidium amansii for butyric acid production with ph control
Bioprocess and Biosystems Engineering, 2017Co-Authors: Gwitaek Jeong, Sungkoo KimAbstract:Optimal hyper-thermal (HT) acid hydrolysis conditions for Gelidium amansii were determined to be 12% (w/v) seaweed slurry content and 144 mM H2SO4 at 150 °C for 10 min. HT acid hydrolysis-treated G. amansii hydrolysates produced low concentrations of inhibitory compounds and adsorption treatment using 3% activated carbon. An adsorption time of 5 min was subsequently used to remove the inhibitory 5-hydroxymethylfurfural from the medium. A final maximum monosaccharide concentration of 44.6 g/L and 79.1% conversion from 56.4 g/L total fermentable monosaccharides with 120 g dw/L G. amansii slurry was obtained from HT acid hydrolysis, enzymatic saccharification, and adsorption treatment. This study demonstrates the potential for butyric acid production from G. amansii hydrolysates under non-pH-controlled as well as pH-controlled fermentation using Clostridium acetobutylicum KCTC 1790. The activated carbon treatment and pH-controlled fermentation showed synergistic effects and produced butyric acid at a concentration of 11.2 g/L after 9 days of fermentation.
Gwitaek Jeong - One of the best experts on this subject based on the ideXlab platform.
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evaluation of 2 3 butanediol production from red seaweed Gelidium amansii hydrolysates using engineered saccharomyces cerevisiae
Journal of Microbiology and Biotechnology, 2020Co-Authors: Jinho Seo, Gwitaek Jeong, Sungkoo KimAbstract:Hyper-thermal (HT) acid hydrolysis of red seaweed Gelidium amansii was performed using 12% (w/v) slurry and an acid mix concentration of 180 mM at 150°C for 10 min. Enzymatic saccharification when using a combination of Celluclast 1.5 L and CTec2 at a dose of 16 U/ml led to the production of 12.0 g/l of reducing sugar with an efficiency of enzymatic saccharification of 13.2%. After the enzymatic saccharification, 2,3-butanediol (2,3-BD) fermentation was carried out using an engineered S. cerevisiae strain. The use of HT acid-hydrolyzed medium with 1.9 g/l of 5-hydroxymethylfurfural showed a reduction in the lag time from 48 to 24 h. The 2,3-BD concentration and yield coefficient at 72 h were 14.8 g/l and 0.30, respectively. Therefore, HT acid hydrolysis and the use of the engineered S. cerevisiae strain can enhance the overall 2,3-BD yields from G. amansii seaweed.
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evaluation of 2 3 butanediol production from red seaweed Gelidium amansii hydrolysates using engineered saccharomyces cerevisiae
Journal of Microbiology and Biotechnology, 2020Co-Authors: Jinho Seo, Gwitaek Jeong, Sungkoo KimAbstract:Hyper thermal (HT) acid hydrolysis of red seaweed Gelidium amansii was performed using 12% (w/v) slurry and an acid mix concentration of 180 mM at 150 °C for 10 min. Enzymatic saccharification when using a combination of Celluclast 1.5 L and Ctec2 at a dose of 16 U/mL led to the production of 12.0 g/L of reducing sugar with an the efficiency of enzymatic saccharification (Es) of 13.2%. After the enzymatic saccharification, 2,3-butanediol (2,3-BD) fermentation was carried out using an engineered S. cerevisiae strain. The use of HT acid-hydrolyzed medium with 1.9 g/L of 5-HMF showed a reduction in the lag time from 48 to 24 h. The 2,3-BD concentration and yield at 72 h were 14.8 g/L and 0.30, respectively. Therefore, HT acid hydrolysis and the use of the engineered S. cerevisiae strain can enhance the overall 2,3-BD yields from G. amansii seaweed.
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detoxification of hydrolysates of the red seaweed Gelidium amansii for improved bioethanol production
Applied Biochemistry and Biotechnology, 2019Co-Authors: Trung Hau Nguyen, Gwitaek Jeong, In Yung Sunwoo, Sungkoo KimAbstract:In this study, bioethanol was produced from the seaweed Gelidium amansii as biomass through separate hydrolysis and fermentation (SHF) processes. The SHF processes examined in this study include thermal acid hydrolysis pretreatment, enzymatic saccharification, detoxification, and fermentation. Thermal acid hydrolysis pretreatment was conducted using H2SO4, with a slurry content of 8–16% and treatment time of 15–75 min. The optimal conditions for thermal acid hydrolysis pretreatment were 12% (w/v) seaweed slurry content and 180 mM H2SO4 at 121 °C for 45 min, at which 26.1 g/L galactose and 6.8 g/L glucose were produced. A monosaccharide (mainly glucose) was also obtained from the enzymatic saccharification of thermal acid hydrolysate using 16 U/mL Celluclast 1.5 L enzyme at 45 °C for 36 h. Detoxification was performed using the adsorption method with activated carbon, the overliming method with Ca (OH)2, and the ion exchange method with polyethyleneimine. Among those detoxification methods, activated carbon showed the best performance for hydroxymethylfurfural removal. Ethanol fermentation was performed using 12% (w/v) seaweed hydrolysate with Saccharomyces cerevisiae adapted to galactose as well as various detoxification treatments.
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improved fermentation performance to produce bioethanol from Gelidium amansii using pichia stipitis adapted to galactose
Bioprocess and Biosystems Engineering, 2018Co-Authors: Pailin Sukwong, Gwitaek Jeong, In Yung Sunwoo, Sumate Tantratian, Sungkoo KimAbstract:This study employed a statistical method to obtain optimal hyper thermal acid hydrolysis conditions using Gelidium amansii (red seaweed) as a source of biomass. The optimal hyper thermal acid hydrolysis using G. amansii as biomass was determined as 12% (w/v) slurry content, 358.3 mM H2SO4, and temperature of 142.6 °C for 11 min. After hyper thermal acid hydrolysis, enzymatic saccharification was carried out. The total monosaccharide concentration was 45.1 g/L, 72.2% of the theoretical value of the total fermentable monosaccharides of 62.4 g/L based on 120 g dry weight/L in the G. amansii slurry. To increase ethanol production, 3.8 g/L 5-hydroxymethylfurfural (HMF) in the hydrolysate was removed by treatment with 3.5% (w/v) activated carbon for 2 min and fermented with Pichia stipitis adapted to high galactose concentrations via separate hydrolysis and fermentation. With complete HMF removal and the use of P. stipitis adapted to high galactose concentrations, 22 g/L ethanol was produced (yield 0.50). Fermentation with total HMF removal and yeast adapted to high galactose concentrations increased the fermentation performance and decreased the fermentation time from 96 to 36 h compared to traditional fermentation.
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hyper thermal acid hydrolysis and adsorption treatment of red seaweed Gelidium amansii for butyric acid production with ph control
Bioprocess and Biosystems Engineering, 2017Co-Authors: Gwitaek Jeong, Sungkoo KimAbstract:Optimal hyper-thermal (HT) acid hydrolysis conditions for Gelidium amansii were determined to be 12% (w/v) seaweed slurry content and 144 mM H2SO4 at 150 °C for 10 min. HT acid hydrolysis-treated G. amansii hydrolysates produced low concentrations of inhibitory compounds and adsorption treatment using 3% activated carbon. An adsorption time of 5 min was subsequently used to remove the inhibitory 5-hydroxymethylfurfural from the medium. A final maximum monosaccharide concentration of 44.6 g/L and 79.1% conversion from 56.4 g/L total fermentable monosaccharides with 120 g dw/L G. amansii slurry was obtained from HT acid hydrolysis, enzymatic saccharification, and adsorption treatment. This study demonstrates the potential for butyric acid production from G. amansii hydrolysates under non-pH-controlled as well as pH-controlled fermentation using Clostridium acetobutylicum KCTC 1790. The activated carbon treatment and pH-controlled fermentation showed synergistic effects and produced butyric acid at a concentration of 11.2 g/L after 9 days of fermentation.
Xueyan Wang - One of the best experts on this subject based on the ideXlab platform.
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enzymatic desulfation of the red seaweeds agar by marinomonas arylsulfatase
International Journal of Biological Macromolecules, 2016Co-Authors: Xueyan Wang, Delin Duan, Xiaoting FuAbstract:Agar and sulfated galactans were isolated from the red seaweeds Gracilariopsis lemaneiformis and Gelidium amansii. A previously purified arylsulfatase from Marinomonas sp. FW-1 was used to remove sulfate groups in agar and sulfated galactans. After enzymatic desulfation, the sulfate content decreased to about 0.16% and gel strength increased about two folds. Moreover, there was no difference between the DNA electrophoresis spectrum on the gel of the arylsulfatase-treated agar and that of the commercial agarose. In order to reveal the desulfation ratio and site, chemical and structural identification of sulfated galactan were carried out. G. amansii sulfated galactan with 7.4% sulfated content was composed of galactose and 3,6-anhydro-L-galactose. Meanwhile, G. lemaneiformis sulfated galactan with 8.5% sulfated content was composed of galactose, 3,6-anhydro-L-galactose, 2-O-methyl-3,6-anhydro-L-galactose and xylose. Data from C-13 NMR, FT-IR, GC-MS provided evidence of sulfate groups at C-4 and C-6 of d-galactose and C-6 of I-galactose both in GRAP and GEAP. Data from GC-MS revealed that desulfation was carried out by the arylsulfatase at the sulfate bonds at C-4 and C-6 of d-galactose and C-6 of l-galactose, with a desulfation ratio of 83.4% and 86.0% against GEAP and GRAP, respectively. (C) 2016 Elsevier B.V. All rights reserved.
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enzymatic desulfation of the red seaweeds agar by marinomonas arylsulfatase
International Journal of Biological Macromolecules, 2016Co-Authors: Xueyan Wang, Delin DuanAbstract:Agar and sulfated galactans were isolated from the red seaweeds Gracilariopsis lemaneiformis and Gelidium amansii. A previously purified arylsulfatase from Marinomonas sp. FW-1 was used to remove sulfate groups in agar and sulfated galactans. After enzymatic desulfation, the sulfate content decreased to about 0.16% and gel strength increased about two folds. Moreover, there was no difference between the DNA electrophoresis spectrum on the gel of the arylsulfatase-treated agar and that of the commercial agarose. In order to reveal the desulfation ratio and site, chemical and structural identification of sulfated galactan were carried out. G. amansii sulfated galactan with 7.4% sulfated content was composed of galactose and 3,6-anhydro-l-galactose. Meanwhile, G. lemaneiformis sulfated galactan with 8.5% sulfated content was composed of galactose, 3,6-anhydro-l-galactose, 2-O-methyl-3,6-anhydro-l-galactose and xylose. Data from 13C NMR, FT-IR, GC-MS provided evidence of sulfate groups at C-4 and C-6 of d-galactose and C-6 of l-galactose both in GRAP and GEAP. Data from GC-MS revealed that desulfation was carried out by the arylsulfatase at the sulfate bonds at C-4 and C-6 of d-galactose and C-6 of l-galactose, with a desulfation ratio of 83.4% and 86.0% against GEAP and GRAP, respectively.
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characterization of a novel alkaline arylsulfatase from marinomonas sp fw 1 and its application in the desulfation of red seaweed agar
Journal of Industrial Microbiology & Biotechnology, 2015Co-Authors: Xueyan Wang, Jiachao Xu, Delin Duan, Xin Gao, Xiaoting FuAbstract:A bacterial strain capable of hydrolyzing sulfate ester bonds of p-nitrophenyl sulfate (pNPS) and agar was isolated from the coast area of Qingdao, China. It was identified as Marinomonas based on its 16S rRNA gene sequence and named as Marinomonas sp. FW-1. An arylsulfatase with a recovery of 13 % and a fold of 12 was purified to a homogeneity using ion exchange and gel filtration chromatographies. The enzyme was composed of a single polypeptide chain with the molecular mass of 33 kDa estimated using SDS-PAGE. The optimal pH and temperature of arylsulfatase were pH 9.0 and 45, respectively. Arylsulfatase was stable over pH 8–11 and at temperature below 55 °C. The K m and V max of this enzyme for the hydrolysis of pNPS were determined to be 13.73 and 270.27 μM/min, respectively. The desulfation ratio against agar from red seaweed Gelidium amansii and Gracilaria lemaneiformis were 86.11 and 89.61 %, respectively. There was no difference between the DNA electrophoresis spectrum on the gel of the arylsulfatase-treated G. amansii agar and that of the commercial agarose. Therefore, this novel alkaline arylsulfatase might have a great potential for application in enzymatic conversion of agar to agarose.
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characterization of a novel alkaline arylsulfatase from marinomonas sp fw 1 and its application in the desulfation of red seaweed agar
Journal of Industrial Microbiology & Biotechnology, 2015Co-Authors: Xueyan Wang, Delin Duan, Xin GaoAbstract:A bacterial strain capable of hydrolyzing sulfate ester bonds of p-nitrophenyl sulfate (pNPS) and agar was isolated from the coast area of Qingdao, China. It was identified as Marinomonas based on its 16S rRNA gene sequence and named as Marinomonas sp. FW-1. An arylsulfatase with a recovery of 13 % and a fold of 12 was purified to a homogeneity using ion exchange and gel filtration chromatographies. The enzyme was composed of a single polypeptide chain with the molecular mass of 33 kDa estimated using SDS-PAGE. The optimal pH and temperature of arylsulfatase were pH 9.0 and 45, respectively. Arylsulfatase was stable over pH 8-11 and at temperature below 55 A degrees C. The K (m) and V (max) of this enzyme for the hydrolysis of pNPS were determined to be 13.73 and 270.27 mu M/min, respectively. The desulfation ratio against agar from red seaweed Gelidium amansii and Gracilaria lemaneiformis were 86.11 and 89.61 %, respectively. There was no difference between the DNA electrophoresis spectrum on the gel of the arylsulfatase-treated G. amansii agar and that of the commercial agarose. Therefore, this novel alkaline arylsulfatase might have a great potential for application in enzymatic conversion of agar to agarose.
Jeongjun Yoon - One of the best experts on this subject based on the ideXlab platform.
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use of red algae ceylon moss Gelidium amansii hydrolyzate for clostridial fermentation
Biomass & Bioenergy, 2013Co-Authors: Younga Kim, Myung Kyo Shin, Jeongjun Yoon, Yong Jin Kim, Daehee Kim, Taeyoung Kim, In Seop ChangAbstract:Marine macroalgae, Ceylon moss containing low lignin and high levels of fermentable sugar (42%), was tested as a potential fermentation feedstock for solventogenic clostridia (Clostridium tetanomorphum ATCC49273, Clostridium acetobutylicum ATCC824, Clostridium aurantibutyricum NCIMB10659, and Clostridium beijerinckii NCIMB8052). As a major carbohydrate of pretreated Ceylon moss (hydrolyzate of Ceylon moss, hCM; hydrolyzate of Agar, hA) is a galactose, four strains were tested to verify galactose availability: C. tetanomorphum was screened out, because it could not utilize galactose. The other strains were then cultivated with hCM or hA. As a result, C. acetobutylicum and C. aurantibutyricum showed bacterial growth with consumption of galactose in both hCM and hA, whereas C. beijerinckii did not grow on hA, possibly due to susceptibility to inhibitor(s) existed. Fatty acids and solvents were produced; substantiating the fact that Ceylon moss can be used as a biomass resource for biochemicals production.
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Optimization of batch dilute-acid hydrolysis for biohydrogen production from red algal biomass
International Journal of Hydrogen Energy, 2013Co-Authors: Jeong Hoon Park, Jeongjun Yoon, Hyo Chang Cheon, Hee-deung Park, Sang-hyoun KimAbstract:Abstract Marine algae are promising alternative sources for bioenergy including hydrogen. Their polymeric structure, however, requires a pretreatment such as dilute-acid hydrolysis prior to fermentation. This study aimed to optimize the control variables of batch dilute-acid hydrolysis for dark hydrogen fermentation of algal biomass. The powder of Gelidium amansii was hydrolyzed at temperatures of 120–180 °C, solid/liquid (S/L) ratios of 5–15% (w/v), and H 2 SO 4 concentrations of 0.5–1.5% (w/w), and then fed to batch hydrogen fermentation. Among the three control variables, hydrolysis temperature was the most significant for hydrogen production as well as for hydrolysis efficiency. The maximum hydrogen production performance of 0.51 L H 2 /L/hr and 37.0 mL H 2 /g dry biomass was found at 161–164 °C hydrolysis temperature, 12.7–14.1% S/L ratio, and 0.50% H 2 SO 4 . The optimized dilute-acid hydrolysis would enhance the feasibility of the red algal biomass as a suitable substrate for hydrogen fermentation.
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use of Gelidium amansii as a promising resource for bioethanol a practical approach for continuous dilute acid hydrolysis and fermentation
Bioresource Technology, 2012Co-Authors: Jeong Hoon Park, Jiyeon Hong, Hyun Chul Jang, Seung Geun Oh, Jeongjun YoonAbstract:A facile continuous method for dilute-acid hydrolysis of the representative red seaweed species, Gelidium amansii was developed and its hydrolysate was subsequently evaluated for fermentability. In the hydrolysis step, the hydrolysates obtained from a batch reactor and a continuous reactor were systematically compared based on fermentable sugar yield and inhibitor formation. There are many advantages to the continuous hydrolysis process. For example, the low melting point of the agar component in G. amansii facilitates improved raw material fluidity in the continuous reactor. In addition, the hydrolysate obtained from the continuous process delivered a high sugar and low inhibitor concentration, thereby leading to both high yield and high final ethanol titer in the fermentation process.
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feasibility of biohydrogen production from Gelidium amansii
International Journal of Hydrogen Energy, 2011Co-Authors: Jeong Hoon Park, Jeongjun Yoon, Hee-deung Park, Yong Jin Kim, Dong Jung Lim, Sang-hyoun KimAbstract:Abstract The feasibility of hydrogen production from red algae was investigated. Galactose, the main sugar monomer of red algae, was readily converted to hydrogen by dark fermentation. The maximum hydrogen production rate and yield of galactose were 2.46 L H2/g VSS/d and 2.03 mol H2/mol galactoseadded, respectively, which were higher than those for glucose (0.914 L H2/g VSS/d and 1.48 mol H2/mol galactoseadded). The distribution of soluble byproducts showed that H2 production was the main pathway of galactose uptake. 5-HMF, the main byproduct of acid hydrolysis of red algae causes noncompetitive inhibition of H2 fermentation. 1.37 g/L of 5-HMF decreased hydrogen production rate by 50% compared to the control. When red algae was hydrolyzed at 150 °C for 15 min and detoxified by activated carbon, 53.5 mL of H2 was produced from 1 g of dry algae with a hydrogen production rate of 0.518 L H2/g VSS/d. Red algae, cultivable on vast tracts of sea by sunlight without any nitrogen-based fertilizer, could be a suitable substrate for biohydrogen production.
Delin Duan - One of the best experts on this subject based on the ideXlab platform.
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enzymatic desulfation of the red seaweeds agar by marinomonas arylsulfatase
International Journal of Biological Macromolecules, 2016Co-Authors: Xueyan Wang, Delin Duan, Xiaoting FuAbstract:Agar and sulfated galactans were isolated from the red seaweeds Gracilariopsis lemaneiformis and Gelidium amansii. A previously purified arylsulfatase from Marinomonas sp. FW-1 was used to remove sulfate groups in agar and sulfated galactans. After enzymatic desulfation, the sulfate content decreased to about 0.16% and gel strength increased about two folds. Moreover, there was no difference between the DNA electrophoresis spectrum on the gel of the arylsulfatase-treated agar and that of the commercial agarose. In order to reveal the desulfation ratio and site, chemical and structural identification of sulfated galactan were carried out. G. amansii sulfated galactan with 7.4% sulfated content was composed of galactose and 3,6-anhydro-L-galactose. Meanwhile, G. lemaneiformis sulfated galactan with 8.5% sulfated content was composed of galactose, 3,6-anhydro-L-galactose, 2-O-methyl-3,6-anhydro-L-galactose and xylose. Data from C-13 NMR, FT-IR, GC-MS provided evidence of sulfate groups at C-4 and C-6 of d-galactose and C-6 of I-galactose both in GRAP and GEAP. Data from GC-MS revealed that desulfation was carried out by the arylsulfatase at the sulfate bonds at C-4 and C-6 of d-galactose and C-6 of l-galactose, with a desulfation ratio of 83.4% and 86.0% against GEAP and GRAP, respectively. (C) 2016 Elsevier B.V. All rights reserved.
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enzymatic desulfation of the red seaweeds agar by marinomonas arylsulfatase
International Journal of Biological Macromolecules, 2016Co-Authors: Xueyan Wang, Delin DuanAbstract:Agar and sulfated galactans were isolated from the red seaweeds Gracilariopsis lemaneiformis and Gelidium amansii. A previously purified arylsulfatase from Marinomonas sp. FW-1 was used to remove sulfate groups in agar and sulfated galactans. After enzymatic desulfation, the sulfate content decreased to about 0.16% and gel strength increased about two folds. Moreover, there was no difference between the DNA electrophoresis spectrum on the gel of the arylsulfatase-treated agar and that of the commercial agarose. In order to reveal the desulfation ratio and site, chemical and structural identification of sulfated galactan were carried out. G. amansii sulfated galactan with 7.4% sulfated content was composed of galactose and 3,6-anhydro-l-galactose. Meanwhile, G. lemaneiformis sulfated galactan with 8.5% sulfated content was composed of galactose, 3,6-anhydro-l-galactose, 2-O-methyl-3,6-anhydro-l-galactose and xylose. Data from 13C NMR, FT-IR, GC-MS provided evidence of sulfate groups at C-4 and C-6 of d-galactose and C-6 of l-galactose both in GRAP and GEAP. Data from GC-MS revealed that desulfation was carried out by the arylsulfatase at the sulfate bonds at C-4 and C-6 of d-galactose and C-6 of l-galactose, with a desulfation ratio of 83.4% and 86.0% against GEAP and GRAP, respectively.
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characterization of a novel alkaline arylsulfatase from marinomonas sp fw 1 and its application in the desulfation of red seaweed agar
Journal of Industrial Microbiology & Biotechnology, 2015Co-Authors: Xueyan Wang, Jiachao Xu, Delin Duan, Xin Gao, Xiaoting FuAbstract:A bacterial strain capable of hydrolyzing sulfate ester bonds of p-nitrophenyl sulfate (pNPS) and agar was isolated from the coast area of Qingdao, China. It was identified as Marinomonas based on its 16S rRNA gene sequence and named as Marinomonas sp. FW-1. An arylsulfatase with a recovery of 13 % and a fold of 12 was purified to a homogeneity using ion exchange and gel filtration chromatographies. The enzyme was composed of a single polypeptide chain with the molecular mass of 33 kDa estimated using SDS-PAGE. The optimal pH and temperature of arylsulfatase were pH 9.0 and 45, respectively. Arylsulfatase was stable over pH 8–11 and at temperature below 55 °C. The K m and V max of this enzyme for the hydrolysis of pNPS were determined to be 13.73 and 270.27 μM/min, respectively. The desulfation ratio against agar from red seaweed Gelidium amansii and Gracilaria lemaneiformis were 86.11 and 89.61 %, respectively. There was no difference between the DNA electrophoresis spectrum on the gel of the arylsulfatase-treated G. amansii agar and that of the commercial agarose. Therefore, this novel alkaline arylsulfatase might have a great potential for application in enzymatic conversion of agar to agarose.
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characterization of a novel alkaline arylsulfatase from marinomonas sp fw 1 and its application in the desulfation of red seaweed agar
Journal of Industrial Microbiology & Biotechnology, 2015Co-Authors: Xueyan Wang, Delin Duan, Xin GaoAbstract:A bacterial strain capable of hydrolyzing sulfate ester bonds of p-nitrophenyl sulfate (pNPS) and agar was isolated from the coast area of Qingdao, China. It was identified as Marinomonas based on its 16S rRNA gene sequence and named as Marinomonas sp. FW-1. An arylsulfatase with a recovery of 13 % and a fold of 12 was purified to a homogeneity using ion exchange and gel filtration chromatographies. The enzyme was composed of a single polypeptide chain with the molecular mass of 33 kDa estimated using SDS-PAGE. The optimal pH and temperature of arylsulfatase were pH 9.0 and 45, respectively. Arylsulfatase was stable over pH 8-11 and at temperature below 55 A degrees C. The K (m) and V (max) of this enzyme for the hydrolysis of pNPS were determined to be 13.73 and 270.27 mu M/min, respectively. The desulfation ratio against agar from red seaweed Gelidium amansii and Gracilaria lemaneiformis were 86.11 and 89.61 %, respectively. There was no difference between the DNA electrophoresis spectrum on the gel of the arylsulfatase-treated G. amansii agar and that of the commercial agarose. Therefore, this novel alkaline arylsulfatase might have a great potential for application in enzymatic conversion of agar to agarose.