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A Diestre - One of the best experts on this subject based on the ideXlab platform.

  • a survey of pre slaughter conditions halothane Gene Frequency and carcass and meat quality in five spanish pig commercial abattoirs
    Meat Science, 2000
    Co-Authors: M Gispert, L Faucitano, M A Oliver, M D Guardia, C Coll, K Siggens, K Harvey, A Diestre
    Abstract:

    A total of 116 deliveries, comprising 15,695 commercial pigs delivered to five abattoirs, were surveyed during winter and summer. Information about on-farm fasting, transport duration and stocking density, and lairage time was collected. Cortisol, creatine phospho-kinase (CPK), and lactate, and DNA for halothane genotype were analysed in a subsample of pigs at exsanguination in every journey. Electrical conductivity (PQM) in semimembranosus muscle (SM) and carcass characteristics (Fat-o-Meater and skin damage) were measured in each carcass. pHu of SM was analysed in the laboratory in a subsample in every journey. Carcasses were identified as PSE or DFD based on PQM and pHu, respectively. The n Gene Frequency ranged among abattoirs from 54 to 8%. Mean lean content was 58.9% for nn, 57.3% for Nn, and 55.8% for NN pigs, though a difference of 2.5% lean was observed between two abattoirs with the same n Gene Frequency. A straight relationship of the incidence of serious PSE carcasses and n Gene Frequency was found. The overall incidence of serious PSE and DFD carcasses was 6.5 and 12.5%, respectively. A higher incidence of PSE carcasses was found in summer; in deliveries with 0.40 m(2)/100 kg pig; and in transports of 2 h duration; and lairage times >9 h. Cortisol level in blood increased in winter and decreased after 12-18 h fasting time. A rise in the lactate concentration was observed in pigs transported in high stocking density ( 2 h). All blood stress indices increase as increasing lairage time. Carcasses with more skin damage had higher levels of cortisol, CPK and lactate, and higher incidence of DFD meat, compared with non and low skin damage carcasses.

  • a survey of pre slaughter conditions halothane Gene Frequency and carcass and meat quality in five spanish pig commercial abattoirs
    Meat Science, 2000
    Co-Authors: M Gispert, L Faucitano, M A Oliver, M D Guardia, C Coll, K Siggens, K Harvey, A Diestre
    Abstract:

    Abstract A total of 116 deliveries, comprising 15,695 commercial pigs delivered to five abattoirs, were surveyed during winter and summer. Information about on-farm fasting, transport duration and stocking density, and lairage time was collected. Cortisol, creatine phospho-kinase (CPK), and lactate, and DNA for halothane genotype were analysed in a subsample of pigs at exsanguination in every journey. Electrical conductivity (PQM) in semimembranosus muscle (SM) and carcass characteristics (Fat-o-Meater and skin damage) were measured in each carcass. pHu of SM was analysed in the laboratory in a subsample in every journey. Carcasses were identified as PSE or DFD based on PQM and pHu, respectively. The n Gene Frequency ranged among abattoirs from 54 to 8%. Mean lean content was 58.9% for nn, 57.3% for Nn, and 55.8% for NN pigs, though a difference of 2.5% lean was observed between two abattoirs with the same n Gene Frequency. A straight relationship of the incidence of serious PSE carcasses and n Gene Frequency was found. The overall incidence of serious PSE and DFD carcasses was 6.5 and 12.5%, respectively. A higher incidence of PSE carcasses was found in summer; in deliveries with 0.40 m 2 /100 kg pig; and in transports of 2 /100 kg pig; transports of >2 h duration; and lairage times >9 h. Cortisol level in blood increased in winter and decreased after 12–18 h fasting time. A rise in the lactate concentration was observed in pigs transported in high stocking density ( 2 /100 kg pig) and for a longer time (>2 h). All blood stress indices increase as increasing lairage time. Carcasses with more skin damage had higher levels of cortisol, CPK and lactate, and higher incidence of DFD meat, compared with non and low skin damage carcasses.

M Gispert - One of the best experts on this subject based on the ideXlab platform.

  • a survey of pre slaughter conditions halothane Gene Frequency and carcass and meat quality in five spanish pig commercial abattoirs
    Meat Science, 2000
    Co-Authors: M Gispert, L Faucitano, M A Oliver, M D Guardia, C Coll, K Siggens, K Harvey, A Diestre
    Abstract:

    A total of 116 deliveries, comprising 15,695 commercial pigs delivered to five abattoirs, were surveyed during winter and summer. Information about on-farm fasting, transport duration and stocking density, and lairage time was collected. Cortisol, creatine phospho-kinase (CPK), and lactate, and DNA for halothane genotype were analysed in a subsample of pigs at exsanguination in every journey. Electrical conductivity (PQM) in semimembranosus muscle (SM) and carcass characteristics (Fat-o-Meater and skin damage) were measured in each carcass. pHu of SM was analysed in the laboratory in a subsample in every journey. Carcasses were identified as PSE or DFD based on PQM and pHu, respectively. The n Gene Frequency ranged among abattoirs from 54 to 8%. Mean lean content was 58.9% for nn, 57.3% for Nn, and 55.8% for NN pigs, though a difference of 2.5% lean was observed between two abattoirs with the same n Gene Frequency. A straight relationship of the incidence of serious PSE carcasses and n Gene Frequency was found. The overall incidence of serious PSE and DFD carcasses was 6.5 and 12.5%, respectively. A higher incidence of PSE carcasses was found in summer; in deliveries with 0.40 m(2)/100 kg pig; and in transports of 2 h duration; and lairage times >9 h. Cortisol level in blood increased in winter and decreased after 12-18 h fasting time. A rise in the lactate concentration was observed in pigs transported in high stocking density ( 2 h). All blood stress indices increase as increasing lairage time. Carcasses with more skin damage had higher levels of cortisol, CPK and lactate, and higher incidence of DFD meat, compared with non and low skin damage carcasses.

  • a survey of pre slaughter conditions halothane Gene Frequency and carcass and meat quality in five spanish pig commercial abattoirs
    Meat Science, 2000
    Co-Authors: M Gispert, L Faucitano, M A Oliver, M D Guardia, C Coll, K Siggens, K Harvey, A Diestre
    Abstract:

    Abstract A total of 116 deliveries, comprising 15,695 commercial pigs delivered to five abattoirs, were surveyed during winter and summer. Information about on-farm fasting, transport duration and stocking density, and lairage time was collected. Cortisol, creatine phospho-kinase (CPK), and lactate, and DNA for halothane genotype were analysed in a subsample of pigs at exsanguination in every journey. Electrical conductivity (PQM) in semimembranosus muscle (SM) and carcass characteristics (Fat-o-Meater and skin damage) were measured in each carcass. pHu of SM was analysed in the laboratory in a subsample in every journey. Carcasses were identified as PSE or DFD based on PQM and pHu, respectively. The n Gene Frequency ranged among abattoirs from 54 to 8%. Mean lean content was 58.9% for nn, 57.3% for Nn, and 55.8% for NN pigs, though a difference of 2.5% lean was observed between two abattoirs with the same n Gene Frequency. A straight relationship of the incidence of serious PSE carcasses and n Gene Frequency was found. The overall incidence of serious PSE and DFD carcasses was 6.5 and 12.5%, respectively. A higher incidence of PSE carcasses was found in summer; in deliveries with 0.40 m 2 /100 kg pig; and in transports of 2 /100 kg pig; transports of >2 h duration; and lairage times >9 h. Cortisol level in blood increased in winter and decreased after 12–18 h fasting time. A rise in the lactate concentration was observed in pigs transported in high stocking density ( 2 /100 kg pig) and for a longer time (>2 h). All blood stress indices increase as increasing lairage time. Carcasses with more skin damage had higher levels of cortisol, CPK and lactate, and higher incidence of DFD meat, compared with non and low skin damage carcasses.

Hélène Gilbert - One of the best experts on this subject based on the ideXlab platform.

  • A Gene Frequency model for QTL mapping using Bayesian inference
    Genetics selection evolution : GSE, 2010
    Co-Authors: Rohan L. Fernando, Jack C. M. Dekkers, Hélène Gilbert
    Abstract:

    Background Information for mapping of quantitative trait loci (QTL) comes from two sources: linkage disequilibrium (non-random association of allele states) and cosegregation (non-random association of allele origin). Information from LD can be captured by modeling conditional means and variances at the QTL given marker information. Similarly, information from cosegregation can be captured by modeling conditional covariances. Here, we consider a Bayesian model based on Gene Frequency (BGF) where both conditional means and variances are modeled as a function of the conditional Gene frequencies at the QTL. The parameters in this model include these Gene frequencies, additive effect of the QTL, its location, and the residual variance. Bayesian methodology was used to estimate these parameters. The priors used were: logit-normal for Gene frequencies, normal for the additive effect, uniform for location, and inverse chi-square for the residual variance. Computer simulation was used to compare the power to detect and accuracy to map QTL by this method with those from least squares analysis using a regression model (LSR).

  • A Gene Frequency model for QTL mapping using
    2010
    Co-Authors: Rohan L. Fernando, Jack C. M. Dekkers, Hélène Gilbert
    Abstract:

    Background: Information for mapping of quantitative trait loci (QTL) comes from two sources: linkage disequilibrium (non-random association of allele states) and cosegregation (non-random association of allele origin). Information from LD can be captured by modeling conditional means and variances at the QTL given marker information. Similarly, information from cosegregation can be captured by modeling conditional covariances. Here, we consider a Bayesian model based on Gene Frequency (BGF) where both conditional means and variances are modeled as a function of the conditional Gene frequencies at the QTL. The parameters in this model include these Gene frequencies, additive effect of the QTL, its location, and the residual variance. Bayesian methodology was used to estimate these parameters. The priors used were: logit-normal for Gene frequencies, normal for the additive effect, uniform for location, and inverse chi-square for the residual variance. Computer simulation was used to compare the power to detect and accuracy to map QTL by this method with those from least squares analysis using a regression model (LSR). Results: To simplify the analysis, data from unrelated individuals in a purebred population were simulated, where only LD information contributes to map the QTL. LD was simulated in a chromosomal segment of 1 cM with one QTL by random mating in a population of size 500 for 1000 Generations and in a population of size 100 for 50 Generations. The comparison was studied under a range of conditions, which included SNP density of 0.1, 0.05 or 0.02 cM, sample size of 500 or 1000, and phenotypic variance explained by QTL of 2 or 5%. Both 1 and 2-SNP models were considered. Power to detect the QTL for the BGF, ranged from 0.4 to 0.99, and close or equal to the power of the regression using least squares (LSR). Precision to map QTL position of BGF, quantified by the mean absolute error, ranged from 0.11 to 0.21 cM for BGF, and was better than the precision of LSR, which ranged from 0.12 to 0.25 cM. Conclusions: In conclusion given a high SNP density, the Gene Frequency model can be used to map QTL with considerable accuracy even within a 1 cM region.

  • A Gene Frequency model for QTL mapping using Bayesian inference
    Genetics Selection Evolution, 2010
    Co-Authors: Rohan L. Fernando, Jack C. M. Dekkers, Hélène Gilbert
    Abstract:

    Background: Information for mapping of quantitative trait loci (QTL) comes from two sources: linkage disequilibrium (non-random association of allele states) and cosegregation (non-random association of allele origin). Information from LD can be captured by modeling conditional means and variances at the QTL given marker information. Similarly, information from cosegregation can be captured by modeling conditional covariances. Here, we consider a Bayesian model based on Gene Frequency (BGF) where both conditional means and variances are modeled as a function of the conditional Gene frequencies at the QTL. The parameters in this model include these Gene frequencies, additive effect of the QTL, its location, and the residual variance. Bayesian methodology was used to estimate these parameters. The priors used were: logit-normal for Gene frequencies, normal for the additive effect, uniform for location, and inverse chi-square for the residual variance. Computer simulation was used to compare the power to detect and accuracy to map QTL by this method with those from least squares analysis using a regression model (LSR).[br/] Results: To simplify the analysis, data from unrelated individuals in a purebred population were simulated, where only LD information contributes to map the QTL. LD was simulated in a chromosomal segment of 1 cM with one QTL by random mating in a population of size 500 for 1000 Generations and in a population of size 100 for 50 Generations. The comparison was studied under a range of conditions, which included SNP density of 0.1, 0.05 or 0.02 cM, sample size of 500 or 1000, and phenotypic variance explained by QTL of 2 or 5%. Both 1 and 2-SNP models were considered. Power to detect the QTL for the BGF, ranged from 0.4 to 0.99, and close or equal to the power of the regression using least squares (LSR). Precision to map QTL position of BGF, quantified by the mean absolute error, ranged from 0.11 to 0.21 cM for BGF, and was better than the precision of LSR, which ranged from 0.12 to 0.25 cM.[br/] Conclusions: In conclusion given a high SNP density, the Gene Frequency model can be used to map QTL with considerable accuracy even within a 1 cM region.

K Harvey - One of the best experts on this subject based on the ideXlab platform.

  • a survey of pre slaughter conditions halothane Gene Frequency and carcass and meat quality in five spanish pig commercial abattoirs
    Meat Science, 2000
    Co-Authors: M Gispert, L Faucitano, M A Oliver, M D Guardia, C Coll, K Siggens, K Harvey, A Diestre
    Abstract:

    A total of 116 deliveries, comprising 15,695 commercial pigs delivered to five abattoirs, were surveyed during winter and summer. Information about on-farm fasting, transport duration and stocking density, and lairage time was collected. Cortisol, creatine phospho-kinase (CPK), and lactate, and DNA for halothane genotype were analysed in a subsample of pigs at exsanguination in every journey. Electrical conductivity (PQM) in semimembranosus muscle (SM) and carcass characteristics (Fat-o-Meater and skin damage) were measured in each carcass. pHu of SM was analysed in the laboratory in a subsample in every journey. Carcasses were identified as PSE or DFD based on PQM and pHu, respectively. The n Gene Frequency ranged among abattoirs from 54 to 8%. Mean lean content was 58.9% for nn, 57.3% for Nn, and 55.8% for NN pigs, though a difference of 2.5% lean was observed between two abattoirs with the same n Gene Frequency. A straight relationship of the incidence of serious PSE carcasses and n Gene Frequency was found. The overall incidence of serious PSE and DFD carcasses was 6.5 and 12.5%, respectively. A higher incidence of PSE carcasses was found in summer; in deliveries with 0.40 m(2)/100 kg pig; and in transports of 2 h duration; and lairage times >9 h. Cortisol level in blood increased in winter and decreased after 12-18 h fasting time. A rise in the lactate concentration was observed in pigs transported in high stocking density ( 2 h). All blood stress indices increase as increasing lairage time. Carcasses with more skin damage had higher levels of cortisol, CPK and lactate, and higher incidence of DFD meat, compared with non and low skin damage carcasses.

  • a survey of pre slaughter conditions halothane Gene Frequency and carcass and meat quality in five spanish pig commercial abattoirs
    Meat Science, 2000
    Co-Authors: M Gispert, L Faucitano, M A Oliver, M D Guardia, C Coll, K Siggens, K Harvey, A Diestre
    Abstract:

    Abstract A total of 116 deliveries, comprising 15,695 commercial pigs delivered to five abattoirs, were surveyed during winter and summer. Information about on-farm fasting, transport duration and stocking density, and lairage time was collected. Cortisol, creatine phospho-kinase (CPK), and lactate, and DNA for halothane genotype were analysed in a subsample of pigs at exsanguination in every journey. Electrical conductivity (PQM) in semimembranosus muscle (SM) and carcass characteristics (Fat-o-Meater and skin damage) were measured in each carcass. pHu of SM was analysed in the laboratory in a subsample in every journey. Carcasses were identified as PSE or DFD based on PQM and pHu, respectively. The n Gene Frequency ranged among abattoirs from 54 to 8%. Mean lean content was 58.9% for nn, 57.3% for Nn, and 55.8% for NN pigs, though a difference of 2.5% lean was observed between two abattoirs with the same n Gene Frequency. A straight relationship of the incidence of serious PSE carcasses and n Gene Frequency was found. The overall incidence of serious PSE and DFD carcasses was 6.5 and 12.5%, respectively. A higher incidence of PSE carcasses was found in summer; in deliveries with 0.40 m 2 /100 kg pig; and in transports of 2 /100 kg pig; transports of >2 h duration; and lairage times >9 h. Cortisol level in blood increased in winter and decreased after 12–18 h fasting time. A rise in the lactate concentration was observed in pigs transported in high stocking density ( 2 /100 kg pig) and for a longer time (>2 h). All blood stress indices increase as increasing lairage time. Carcasses with more skin damage had higher levels of cortisol, CPK and lactate, and higher incidence of DFD meat, compared with non and low skin damage carcasses.

K Siggens - One of the best experts on this subject based on the ideXlab platform.

  • a survey of pre slaughter conditions halothane Gene Frequency and carcass and meat quality in five spanish pig commercial abattoirs
    Meat Science, 2000
    Co-Authors: M Gispert, L Faucitano, M A Oliver, M D Guardia, C Coll, K Siggens, K Harvey, A Diestre
    Abstract:

    A total of 116 deliveries, comprising 15,695 commercial pigs delivered to five abattoirs, were surveyed during winter and summer. Information about on-farm fasting, transport duration and stocking density, and lairage time was collected. Cortisol, creatine phospho-kinase (CPK), and lactate, and DNA for halothane genotype were analysed in a subsample of pigs at exsanguination in every journey. Electrical conductivity (PQM) in semimembranosus muscle (SM) and carcass characteristics (Fat-o-Meater and skin damage) were measured in each carcass. pHu of SM was analysed in the laboratory in a subsample in every journey. Carcasses were identified as PSE or DFD based on PQM and pHu, respectively. The n Gene Frequency ranged among abattoirs from 54 to 8%. Mean lean content was 58.9% for nn, 57.3% for Nn, and 55.8% for NN pigs, though a difference of 2.5% lean was observed between two abattoirs with the same n Gene Frequency. A straight relationship of the incidence of serious PSE carcasses and n Gene Frequency was found. The overall incidence of serious PSE and DFD carcasses was 6.5 and 12.5%, respectively. A higher incidence of PSE carcasses was found in summer; in deliveries with 0.40 m(2)/100 kg pig; and in transports of 2 h duration; and lairage times >9 h. Cortisol level in blood increased in winter and decreased after 12-18 h fasting time. A rise in the lactate concentration was observed in pigs transported in high stocking density ( 2 h). All blood stress indices increase as increasing lairage time. Carcasses with more skin damage had higher levels of cortisol, CPK and lactate, and higher incidence of DFD meat, compared with non and low skin damage carcasses.

  • a survey of pre slaughter conditions halothane Gene Frequency and carcass and meat quality in five spanish pig commercial abattoirs
    Meat Science, 2000
    Co-Authors: M Gispert, L Faucitano, M A Oliver, M D Guardia, C Coll, K Siggens, K Harvey, A Diestre
    Abstract:

    Abstract A total of 116 deliveries, comprising 15,695 commercial pigs delivered to five abattoirs, were surveyed during winter and summer. Information about on-farm fasting, transport duration and stocking density, and lairage time was collected. Cortisol, creatine phospho-kinase (CPK), and lactate, and DNA for halothane genotype were analysed in a subsample of pigs at exsanguination in every journey. Electrical conductivity (PQM) in semimembranosus muscle (SM) and carcass characteristics (Fat-o-Meater and skin damage) were measured in each carcass. pHu of SM was analysed in the laboratory in a subsample in every journey. Carcasses were identified as PSE or DFD based on PQM and pHu, respectively. The n Gene Frequency ranged among abattoirs from 54 to 8%. Mean lean content was 58.9% for nn, 57.3% for Nn, and 55.8% for NN pigs, though a difference of 2.5% lean was observed between two abattoirs with the same n Gene Frequency. A straight relationship of the incidence of serious PSE carcasses and n Gene Frequency was found. The overall incidence of serious PSE and DFD carcasses was 6.5 and 12.5%, respectively. A higher incidence of PSE carcasses was found in summer; in deliveries with 0.40 m 2 /100 kg pig; and in transports of 2 /100 kg pig; transports of >2 h duration; and lairage times >9 h. Cortisol level in blood increased in winter and decreased after 12–18 h fasting time. A rise in the lactate concentration was observed in pigs transported in high stocking density ( 2 /100 kg pig) and for a longer time (>2 h). All blood stress indices increase as increasing lairage time. Carcasses with more skin damage had higher levels of cortisol, CPK and lactate, and higher incidence of DFD meat, compared with non and low skin damage carcasses.